• Development and Reproduction
• /
• v.2 no.2
• /
• pp.165-171
• /
• 1998

p62 is a novel cytoplsmic protein that binds to SH2 domain of p56$^{lck}$, lymphocyte-specific protein tyrosine kinase, and the expression of p62 was observed in most tissues. In addition p62 interacts with various proteins including ubiquitin and atypical PKC isoform, indicating its diverse biological role in different tissues. However, little is known about functional connection between p62 and its binding proteins. In the present study, a novel cellular protein, p62 has been shown to bind to 14-3-3 $\tau$ isoform that is specific for T cells. Moreover, overexpression of p62 in T cells caused to delay onset of UV-induced apoptosis characterized by DNA fragmentation and breakdown of poly (ADP-ribose) polymerase (PARP). Lately, 14-3-3 proteins have been shown to mediate survival signal via interacting proapoptotic Bad protein in the Iymphocyte. These results suggested the presence of p62-mediated regulatory mechanism during apoptosis in T cells, in which activation-induced apoptotic signal could be interfered by p62 and 14-3-3 protein.n.

• Biomedical Science Letters
• /
• v.13 no.2
• /
• pp.75-81
• /
• 2007

Sodium salicylate (NaSal), a chemopreventive drug, has been shown to induce apoptosis and cell circle arrest depending on its concentrations in a variety of cancer cells. In A549 cells, low concentration of NaSal (5$\sim$10 mM) induces cell cycle arrest, whereas it induces apoptosis at higher concentration of 20 mM. In the present study, we examined the molecular mechanism for NaSal-induced cell cycle arrest. NaSal induced expression of p53, p21 (Wafl/Cipl), and p27 (Kipl) that play important roles in cell cycle arrest. p53 induction was mediated by its phosphorylation at Ser-15 that could be prevented by the PI3K-related kinase (ATM, ATR and DNA-PK) inhibitors including wortmannin, caffeine and LY294002. In addition, NaSal-induction of p2l (Wafl/Cipl) was detected in P53 (+/+) wild type A549 cells but not in p53 (-/-) mutant H1299 cells, indicating p53-dependent p21 (Wafl/Cipl) induction. In contrast, p27 (Kipl) that is a negative regulate. of cell cycle with p21 (Wafl/Cipl) was observed both in A549 cells and H1299 cells. Thus, 5 mM NaSal appeared to cause cell cycle arrest through inducing the cyclin-dependent kinase inhibitor p21 (Wafl/Cipl) via PI3K-related protein kinase-dependent p53 activation as well as by up-regulating p27 (Kipl) independently of p53 in A549 cells.

• Biomolecules & Therapeutics
• /
• v.27 no.1
• /
• pp.34-40
• /
• 2019

Transglutaminase 2 (TGase 2) plays a key role in p53 regulation, depleting p53 tumor suppressor through autophagy in renal cell carcinoma. We found that microtubule-associated protein 1A/1B-light chain 3 (LC3), a hallmark of autophagy, were tightly associated with the level of TGase 2 in cancer cells. TGase 2 overexpression increased LC3 levels, and TGase 2 knockdown decreased LC3 levels in cancer cells. Transcript abundance of LC3 was inversely correlated with level of wild type p53. TGase 2 knockdown using siRNA, or TGase 2 inhibition using GK921 significantly reduced autophagy through reduction of LC3 transcription, which was followed by restoration of p53 levels in cancer cells. TGase 2 overexpression promoted the autophagy process by LC3 induction, which was correlated with p53 depletion in cancer cells. Rapamycin-resistant cancer cells also showed higher expression of LC3 compared to the rapamycin-sensitive cancer cells, which was tightly correlated with TGase 2 levels. TGase 2 knockdown or TGase 2 inhibition sensitized rapamycin-resistant cancer cells to drug treatment. In summary, TGase 2 induces drug resistance by potentiating autophagy through LC3 induction via p53 regulation in cancer.

• Korean Journal of Adult Nursing
• /
• v.17 no.4
• /
• pp.656-667
• /
• 2005

Purpose: This study examined the mood state, activities of daily living, and attitude toward aging in the elderly according to the existence of a spouse and investigated the relation between these factors. Method: The subjects consisted of 190 elderly people, who were classified into two groups: those with and without a spouse. The data was collected from March to December 2004 using a structured questionnaire that included general characteristics, mood states, instrumental activities of daily living (IADL), and an attitude toward aging scale. The collected data was analyzed using the program SPSS, including descriptive statistics, $x^2-test$, ANOVA, Scheff's test, and the Pearson correlation coefficient. Result: 1. There were significant differences between the two groups in mood state (t=-3.349, p=.001), anxiety-depression (t=-3.350, p=.001), and anger (t=p=.003) in the mood state subscales, and in attitude toward aging (t=2.514, p=.013). 2. There was a significant positive correlation between vigor and IADL (r=.253, p=.012) in the elderly with a spouse. 3. When there was a spouse, there was a significant difference according to gender (t=2.587, p=.012) in IADL. Without a spouse, there were significant differences in mood states according to education level (F=3.315, p=.023), in anxiety-depression according to the presence of illness (t=2.156, p=.033), in vigor according to age (F=3.439, p=.020) and education level (F=5.285, p=.002), and in IADL according to monthly income (F=3.322, p=.023) and the presence of illness (t=2.172, p=.032). Conclusion: An individualized approach is needed for elderly people that considers living arrangements and the existence of a spouse.

• Journal of Life Science
• /
• v.13 no.3
• /
• pp.280-290
• /
• 2003

The incorporation of RAPD markers into the previous classical and RFLP genetic linkage maps will facilitate the generation of a detailed genetic map by compensating for the lack of one type of marker in the region of interest. The objective of this paper was to present features we observed when we associated RAPD map from an intraspecific cross of a Glycine max$\times$G. max, 'Essex'$\times$PI 437654 with the public RFLP map developed from an interspecific cross of G. max$\times$G. soja. Among 27 linkage groups of RAPD map, eight linkage groups contained probe/enzyme combination RFLP markers, which allowed us the incorporation of RAPD markers into the public RFLP map. Map position rearrangement was observed. In incorporating L.G.C-3 into the public RFLP linkage group a1 and a2, both pSAC3 and pA136 region, and pA170/EcoRV and pB170/HindIII region were in opposite order, respectively. And, pk400 was localized 1.8 cM from pA96-1 and 8.4 cM from pB172 in the public RFLP map, but was localized 9.9 cM from i locus and 18.9 cM from pA85 in our study. A noticeable expansion of the map distances in the intraspecific cross of Essex and PI 437654 was also observed. Map distance between probes pA890 and pK493 in L.G.C-1 was 48.6 cM, but it was only 13.3 cM in the public RFLP map. The distances from the probe pB32-2 to pA670 and from pA670 to pA668 in L.G. C-2 were 50.9 cM and 31.7 cM, but they were 35.9 cM and 13.5 cM in the public RFLP map. The detection of duplicate loci from the same probe that were mapped on the same or/and different linkage group was another feature we observed.

• Microbiology and Biotechnology Letters
• /
• v.24 no.6
• /
• pp.657-663
• /
• 1996

Pseudomonas sp. P20 isolated from the polluted environment is capable of degrading biphenyl and 4-chlorobiphenyl. The pcbABCD genes responsible for degradation of biphenyl and 4-chlorobiphenyl were cloned using pBluescript SK(+) from the chromosomal DNA of Pseudomonas sp. P20 to construct pCK1 and pCK102, harbouring pcbABCD and pcbCD, respectively. The 2, 3-DHBP dioxygenase gene, pcbC, was cloned again from pCK102 by using pKT230 which is known as a shuttle vector and pKK1 hybrid plasmid was constructed. The E. coli KK1 transformant obtained by transforming the pKK1 into E. coli XL1-Blue showed 2, 3-DHBP dioxygenase activity. The specific 2, 3-DHBP dioxygenase activity of E. coli KK1 was similar to that of the E. coli CK102, but much higher than those of the natural isolates, Pseudomonas sp. DJ-12 and Pseudomonas sp. P20.

• Journal of Life Science
• /
• v.31 no.11
• /
• pp.995-1003
• /
• 2021

Chromatin remodeling regulates gene expression through epigenetic mechanisms. Aberrations in histone modification have been associated with depression-like behaviors in animal models. Additionally, growing evidence also indicates that epigenetic modification is associated with depression. p11 (S100A10) has been implicated in the pathophysiology of depression both in human and rodent models. In the present study, we investigated alterations in histone acetylation and methylation at the promoter of the p11 gene in the hippocampus of mice subjected to chronic unpredictable stress (CUS). C57BL/6 mice were exposed to CUS daily for 3 weeks. Depression-like behaviors were measured with the forced swimming test (FST). The levels of hippocampal p11 expression were analyzed by quantitative real-time polymerase chain reaction (PCR) and Western blotting. The levels of acetylated and methylated histone H3 at the promoter of p11 were measured by chromatin immunoprecipitation followed by real-time PCR. CUS-exposed mice displayed depression-like behaviors with prolonged immobility in FST. CUS led to significant decreases in the expression of p11 at both protein and mRNA levels. Meanwhile, there was a decrease in histone H3 acetylation (Ac-H3) and H3-K4 trimethylation (H3K4met3) and an increase in H3-K27 trimethylation (H3K27met3) at the p11 promoter. These results indicate that chronic stress causes the epigenetic suppression of p11 expression in the hippocampus.

• Bulletin of the Korean Chemical Society
• /
• v.14 no.2
• /
• pp.283-287
• /
• 1993

p-(2,2,3,3-Tetracyanocyclopropyl)phenyl acrylate (3a) and p-(2,2,3,3-tetracyanocyclopropyl)phenyl methacrylate (3b) were prepared by the reactions of bromomalononitrile with p-acryloyloxybenzylidenemalononitrile (2a) or p-methacryloyloxy-benzylidenemalononitrile (2b), respectively. Compounds 3a and 3b were polymerized with free radical initiators to obtain the polymers with multicyano functionalities in the cyclopropane ring. The resulting polymer 4a was soluble in acetone but the polymer 4b was not soluble in common solvents. The inherent viscosities of polymers 4a were in the range of 0.10-0.15 dL/g in acetone and those of 4b were in the range of 0.20-0.30 dL/g in 98% sulfuric acid. Solution-cast films were cloudy and brittle, showing $T_g$ values in the range of 106-125$^{\circ}$C.

• Korean Journal of Poultry Science
• /
• v.37 no.2
• /
• pp.145-150
• /
• 2010

This study was conducted to evaluate effects of dietary Agariemycetes on egg quality and fecal noxious gas concentration in laying hens. A total of two hundred forty laying hens were randomly allocated to four treatments with five replicates of twelve chicks per pen. The experiment lasted 42 days with a 7-day adjustment period. Dietary treatments were as follows: 1) CON (basal diet), 2) A1 (basal diet + Agariemycetes 0.1%), 3) A3 (basal diet + Agariemycetes 0.3%) and 4) A5 (basal diet + Agariemycetes 0.5%). There was no significant difference in egg production and egg weight between CON and Agariemycetes treatments. During the second week of the experiment, eggshell breaking strength was higher in A3 and A5 treatments than that in CON treatment (P<0.05). Besides, eggshell breaking strength in P5 treatment was higher when compared with that in CON treatment (P<0.05) in the sixth week. During the second week of the experiment, eggshell thickness was higher in Agariemycetes treatments than CON treatment (P<0.05). Also, A5 treatment was higher than other treatments in the fourth week of the experiment (P<0.05). At the end of the second week, yolk color unit in A5 treatment was lower compared with CON treatment (P<0.05). During the fourth week of the experiment, CON treatment was higher than A3 treatment (P<0.05). During the second week of the experiment, Haugh unit in A3 treatment was greater than that in CON treatment (P<0.05). At the end of six weeks, Haugh unit in CON treatment was lower than other treatments (P<0.05). Fecal ammonia and hydrogen sulfide were increased in Agariemycetes treatments when compared with CON treatment (P<0.05). Compared with CON and A5 treatments, fecal mercaptans was higher than in other treatments in first of the experiment (P<0.05). In conclusion, this study indicated that the addition of Agariemycetes in the diet improved the egg quality and decreased the feacal noxious gas concentration in laying hens.

• Biomedical Science Letters
• /
• v.16 no.4
• /
• pp.307-310
• /
• 2010

Apoptosis is an important significance in the pathogenesis of cancer. Caspase 3 and p53 have been identified as important members of the apoptosis related proteins. This study was performed to define roles of caspase 3 expression and its relationship with p53 expression in endometrial cancers by immunohistochemistry. Immunoreactivity for caspase 3 was found in 13 (65.0%) out of 20 endometrial hyperplasia cases and 8 (36.4%) out of 22 endometrial cancers. Seven (87.5%) of the 8 cases with a positive caspase 3 immunoreactivity showed a positive p53 expression in 22 endometrial cancers. There were no significant associations between caspase 3 and p53 expressions. These findings suggest that caspase 3 expression might be associated with carcinogenesis of endometrial cancers. Further studies are needed to define the relationship between caspase 3 and p53 and apoptosis for examining the mechanisms of tissue-specific apoptosis related protein.