• Korean Journal of Soil Science and Fertilizer
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• v.19 no.4
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• pp.275-282
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• 1986

A series of studies on the properties of clayey terrace soils distributed at the inland (Yeongcheon) and coastal (Yeongjil) regions in Yeongnam district was carried out. On the base of the facts found and already reported about the macro morphological features as well as on pedological characters in micro scale, physicochemical properties, mineralogical characteristics etc., the present study dealt with soil genesis and tried to classify the soils for reasonable use and managements. 1. Although the both regions belonged to "Mesic" soil temperature regime and "red and yellow earths" areas of "Thornthwaite" pedo-climatic diagram, climatic indices as a soil forming factor indicate that the coastal Yeongil had milder than the inland Yeongcheon. 2. All the terrace soils had developed soil profiles with an "Argrllic B". Upyeong soils in Yeongil region had "Argillans" even in the "II B horizons" that possibly be "Paleo-argillic". 3. The bisequum profiles of Bancheon in Yeongcheon and Upyeong in Yeongil revealed that they were developed on Late Mesozoic shale and on semiconsolidated Tertiary deposits respectively, therefore the overlying clayey terrace deposits were assumed to be originated from the Early Quaternary deposits, Diluvium. 4. To supplement the Soil Taxonomy of USDA, the terrace soils with different degrees of gleyzation were classified as follows; Deogpyeong and Hwadong soils which have less than 50cm of paddified gley horizons (redness less than 0.5) in the upper part of the profiles by artificial surface irrigation, tentatively classified into "Anthrepiaquic Hapludalfs" and the Geugrag soils that have more than 50cm of paddified gley horizons within 1.2m of the profiles, into "Anthr-aquic Ochraqualfs" while the Upyeong soils that had greyish mottles in subsoils by natural ground water remain as an "Aquic Hapludalfs" the same as present. The Bancheon soils with free mottles are into "Typic Hapludalfs" as used at present.

• Journal of dental hygiene science
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• v.9 no.3
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• pp.353-360
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• 2009

The purpose of this study was to investigate the recognition for improvement of the Korean Dental Hygienists' Licensing Examination(KDHLE). The subjects were 1,048 dental hygienists were surveyed. either in person or through mail. Data was collected using a self administrated questionnaire from July 1 to July 20, 2008. The data were analysed with frequency and $x^2$-test using the SPSS 15.0 windows. For the inquiry where adequacy of subjects of the KDHLE regarding to evaluation of standard duty achievement ability in the real field was asked, 404(39.5%) answered 'Yes', and 680(66.9%) was answered 'Yes' for necessity of changing current subjects of the KDHLE. For the inquiry about the number of questions in the KDHLE, 353(34.6%) answered that current number of questions is 'Inappropriate' and advisable way of improving KDHLE subjects, 463 (45.4%) answered 'enhancing problem solving ability through unifying subjects', the highest of all. About practical examination methods, 647(67.3%) was answered 'Should be modified'. For methods of dental hygienist practical examination, 'clinical demonstration on patients' received the highest number of votes 628(63.6%). According to the above results, most of active dental hygienists at presence expect the alteration of current KDHLE and new items which are reliable for globalization by testing problem solving ability through unified subjects must be introduced.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.16 no.3
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• pp.321-347
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• 1994

Muscle spindle afferents from masseter muscle were labelled by the intra-axonal HRP injection and were processed for light microscopic reconstruction. Regions containing terminal arbors scattered in the central portion of the masseteric motor neuron pool (type I a) and those restricted to 2-3 small portion of it (type II) were selected and processed for electronmicroscopic analysis with serial sections. The shape of the labelled boutons was dome or elongated shape. Scalloped or glomerulus shape with peripherial indentation containing pre or postsynaptic neuronal propiles, which is occasionally found in the trigeminal main sensory nucleus and spinal dorsal horn, was not observed. Both type Ia and type II boutons had pale axoplasm and contained clear, spherical vesicles of uniform size(dia : 49-52nm) and occasionally large dense cored vesicles(dia : 87-118nm). The synaptic vesicles were evenly distributed throughout the boutons although there was a slight tendency of vesicles to accumulate at the presynaptic site. The average of short and long diameter(short D. + long D./2) of type I a bouton was smaller than that of type II bouton. All the labelled boutons, which showed prominent postsynaptic density, large synaptic area and multiple synaptic contact, made asymmetrical synaptic contact with postsynaptic neuronal propiles. Most of the type Ia and type II boutons made synaptic contact with only one neuronal propile and boutons which shows synaptic contact or more neuronal propiles was not observed. Most of the type Ia boutons(87.2%) were presynaptic to the soma or proximal dendrite and a few remainder(12.8%) made synaptic contact with dendritic shaft or distal dendrite. In contrast, majority of type II boutons showed synaptic contact with dendritic shaft and remainder with soma or proximal dendrite. In conclusion, terminal boutons which participate in the excitatory monosynaptic jaw jerk reflex made synaptic contact with more proximal region of the neuron, and showed very simple synaptic connection, compared with those from the primary afferenst in the other region of the central nervous system such as spinal dorsal horn and trigeminal main sensory nucleus which assumed to be responsible for the mediating pain, tactile sensation, sensory processing or sensory discrimination.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.27-28
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• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.175-184
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• 2016

In this study, we transplanted Chinese (Liaoning Dandong) and Chungnam (Taean) manila clam seeds to Gochang tidal flats in Jeonbuk province, and compared growth, mortality, and condition indices from May 2015 to August 2016. Within 2 months after transplantation, clam mortality of Chungnam and Chinese origin were $6.9{\pm}4.3%$, $16.9{\pm}7.9{\sim}21.0{\pm}6.3%$, respectively. We supposed that higher mortality of Chinese manila clams might be due to higher stress and weakening of physiological functions by air-exposure and unavailability of food intake for 5 days harvesting, transportation, quarantine procedures. Unlike the local clam farmer's opinion based on their field experience, growth of Chungnam clams in shell length and total weight were a little better than Chinese without statistical significant difference (P > 0.05). Condition indices of Chinese clams showed declining tendency after reaching a peak in May 2014, and in June 2015, respectively. Meanwhile, Chungnam clams has reached its peak of condition indices in May 2014 and in April 2015, respectively. From this result, we supposed that there might be at least one month difference in reproduction between the Chinese and Chungnam manila clams during the first-transplanted year. Because the mortality of adult clam have sharply increased from June 2015 (due to high temperature, few rainfall and spawning) in Gochang tidal flat, it would be recommendable that farmers harvest the clams before June when its shell length are larger than 40 mm. In conclusion, we could directly compare the productivity of Chinese and Chungnam manila clam at Gochang tidal flats by field experiment, and these results are also meaningful to manila clam farmers.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.47-56
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• 1999

$^{15}N$-Isotope concentrations of groundwater from l4 wells with different land-use types in Kyonggi Province were measured to investigate the nitrate contamination sources. Water samples were collected monthly from January to December 1997 and analyzed for pH. PC, anions (fluoride, chloride, nitrate, sulfate, inorganic phosphate, and bicarbonate), and canons (calcium, magnesium, potassium, and sodium). For the analysis of the $^{15}N/^{14}N$ ratio as ${\delta}^{15}N$, $N_2$ samples were prepared through Kjeldahl-Rittenberg method and were analyzed using an isotope ratio mass spectrometer (VG Optima IRMS). Reproducibility of the method and precision of the IRMS were below 1.0‰ and 0.1‰, respectively. The ionic composition of each groundwater sample was only slightly different according to the land-use type. The nitrate concentrations of groundwater in cropland or livestock farming areas were higher than those in the residential area. The percentages of nitrate to total anions of groundwater samples from the livestock farming area were higher than those of other areas. The ${\delta}^{15}N$ values of ammonium sulfate, urea, groundwater sample in the non-contaminated area, and water from the animal manure septic tank were -2.7, 1.4, 5.5, and 27.2‰, respectively. Based on the ${\delta}^{15}N$ values, the sources of nitrate could be classified as originated from chemical fertilizers with ${\delta}^{15}N$ values below 5% and as from animal manure or municipal waste with ${\delta}^{15}N$ values over 10‰. In most cases, contamination sources investigated from ${\delta}^{15}N$ values of groundwater samples were correlated with the specific sources according to the land-use types. However, some ${\delta}^{15}N$ values did not matched the apparent land-use types, and there were seasonal variations of ${\delta}^{15}N$ values within the same well. These results suggest that the groundwater quality was affected by two or more contamination sources and the contribution of each source to the groundwater quality varied depending on the sampling season.

• Tuberculosis and Respiratory Diseases
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• v.64 no.1
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• pp.15-21
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• 2008

Background: The melanoma antigen-encoding (MAGE) genes are known to be expressed in various cancer cells, including non-small cell lung cancer (NSCLC), and are silent in all normal tissues except for the testis. In patients with peripheral NSCLC, bronchial washing fluid can be used to detect the MAGE genes, suggesting a diagnosis of lung cancer. In order to evaluate the diagnostic utility of the MAGE test in patients with peripheral NSCLC, bronchial washing fluid was investigated in patients with peripheral pulmonary nodules, which were invisible as detected by bronchoscopy. Methods: Bronchial washing fluid from 37 patients was used for cytological examinations and MAGE gene detection, using RT-nested-PCR of common A1-A6 mRNA. Results were compared to a final diagnosis of patients as confirmed by pathology. Results: Among the 37 subjects, NSCLC was diagnosed in 21 patients, and benign pulmonary diseases were diagnosed in 16 patients. MAGE mRNA was detected in 10 of 21 (47.6%) NSCLC patients, while conventional cytology examinations were positive for MAGE expression in 2 of 21 (9.5%) cases. MAGE expression was observed in 4 of 16 (25%) benign pulmonary disease patients. Conclusion: The MAGE test of bronchial washing fluid can be used as a sensitive predictor of peripheral NSCLC patients.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.1
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• pp.29-39
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• 2004

Objectives: This study was performed to evaluate the laboratory system for successful PGD using fluorescence in situ hybridization (FISH) and the clinical outcome of PGD cycles in five years experiences. Methods: A total of 181 PGD-FISH cycles of 106 couples were performed, and diagnosed chromosome normality in the preimplantation embryos. The laboratory and clinical data were classified by the following optimization steps, and statistically analyzed. Phase I: Blastomere biopsy with two kinds of pipettes, removal of cytoplasmic proteins without treatment of pepsin and culture of biopsied embryos with single medium; Phase II: Blatomere biopsy with single pipette, removal of cytoplasmic proteins with pepsin and culture of biopsied embryos with single medium; Phase III: Blastomere biopsy with single pipette, removal of cytoplasmic proteins with pepsin and culture of biopsied embryos with sequential media. Results: A total of 3, 209 oocytes were collected, and 83.8% (2, 212/2, 640) of fertilization rate was obtained by ICSI procedure. The successful blastomere biopsies were accomplished in 98.6% (2, 043/2, 071) of embryos, and the successful diagnosis rate of FISH was 94.7% (1, 935/ 2, 043) of blastomeres from overall data. Embryo transfers with normal embryos were conducted in 93.9% (170/181) of started cycles. There was no difference in the successful rate of biopsy and diagnosis among Phase I, II and III. However, the pregnancy rate per embryo transfer of Phase III (38.8%, 26/67) was significantly (p<0.05) higher than those of Phase I (13.9%, 5/36) and Phase II (14.9%, 10/67). Conclusions: The laboratory optimization and experience for the PGD with FISH procedure can increase the pregnancy rate to 38.8% in the human IVF-ET program. Our facility of PGD with FISH provides the great possibility to get a normal pregnancy for the concerned couples by chromosomal aberrations.

• Radiation Oncology Journal
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• v.10 no.1
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• pp.35-41
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• 1992

Forty eight patients with adenoid cystic carcinoma (ACC) in the head and neck treated between 1979 and 1990 were reviewed according to treatment modalities and clinical courses. The common site of origin was minor salivary gland ($69\%$), mostly hard palate and maxillary sinus. 11 patients received radiation therapy (RT) alone and 37 patients received combined surgery and radiation therapy. The follow-up period of the survivors ranged from 4 to 118 months (median 49.5 months). The 5 year local control rate was $69.3\%$, $67.3\%$ and 83.9$\%$ in RT alone, conservative operation (OP)+RT and radical OP+RT group, respectively (p>0.05). The control of local disease was best achieved with radical OP+RT. In postoperative RT, high dose ($\ge$60 Gy) and generous field size($\ge$64 cm$^2$) yielded a better local control rate. Actuarial overall survival rate was $79.0\%$, at 5 years and $19.2\%$ at 10 years. Distant metastases (DM) developed in $40\%$ of patients, mostly in the lung. Disease-free (NED) survival rate was $45.8\%$ at 5 years and significant differences did not exist according to primary sites and treatment options. Survival rate after the onset of DM was $19.5\%$ at 5 years. Occurrence of DM tends to lower survival significantly. In an effort to find the role of RT in the treatment of ACC, our data suggest that a well-planned postoperative RT employing a high dose and generous field size can produce high local control rate and remaining urgent problem of distant metastasis demands more effective chemotherapy for the purpose of improving survival of ACC patients.

• Applied Biological Chemistry
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• v.47 no.1
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• pp.78-84
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• 2004

In order Nuruk to improve the quality of millet wine, a traditional Jeju cereal wine, yeasts and molds were isolated from 35 kinds of Nuruk collected nationwide. Isolated strains were screened for saccharification of starch and brewing of millet wine. Fermentation characteristics of millet wine with different types of Nuruk were also investigated. The average number of microbial populations in the Nuruk were $6.4{\times}10^5{\sim}4.5{\times}10^7\;cfu/g$ for molds and $1.4{\times}10^4{\sim}7.7{\times}10^7\;cfu/g$ for yeasts. Among the 169 strains of molds and 103 strains of yeasts, 16 strains were screened for saccharifying activity on starch as a substrate, and one yeast strain was screened for the brewing of millet wine. A8-3, supposed as Aspergillus sp., showed the highest enzyme activities of glucdamylase, ${\alpha}-amylase$ and xylanase while B23-3 strain, supposed as Rhizopus sp., showed the highest saccharifying activity. A10-4, supposed as Saccharomyces sp., showed the highest level of weight loss from $CO_2$ evolution, sugar and alcohol tolerance during fermentation. When the Nuruk was made after inoculation with the selected strains, saccharifying activity was higher for the co-cultivation of A8-3 and B23-3 than individual cultivation of each strain. Similar saccharifying activities were shown in both disc-type and pellet-type Nuruk. It was suggested that pellet-type Nuruk could improve fermentation yield. The collected Nuruk consisted of $10{\sim}13%$ moisture, $55{\sim}70%$ total sugar, $10{\sim}18%$ crude protein, $0.2{\sim}1.0%$ crude fat and $1.8{\sim}2.1%$ ash. The Nuruk made in this study was composed of $12{\sim}15%$ moisture, $61{\sim}71%$ total sugar, $15{\sim}20%$ crude protein, $0.4{\sim}1.5%$ crude fat and $1.1{\sim}1.5%$ ash.