• Journal of Life Science
• /
• v.12 no.3
• /
• pp.242-249
• /
• 2002

The optimal culture conditions in 500$m\ell$ flask suture, 5$\ell$ jar fermenter and 2,000 $\ell$ large stale culture were investigated to maximize the production of antibiotic on Rhizoctonia solani AC4, the causal agent of vegetables damping-off, by the strain Bacillus ehimensis YJ-37. Starch (1.5%) as a carbon source, peptone (0.6%) as a nitrogen source and MgC1$_2$(0.15%) as a metal ion in the medium containing N $a_2$HP $O_4$(0.3%) showed the highest production of the antibiotic(s) in a rotary shake (200 rpm). Optimal initial pH of the culture medium, culture temperature and culture time for the antibiotic(s) production were pH 8.0, 32$^{\circ}C$ and 54hrs, respertively. Under the optimal renditions in flask culture, cell growth and antifungal activity (clear zone size) were 1.42 $\times$ 10$^{8}$ cfu/$m\ell$ (21g-DCW/ $\ell$) and 13.9 mm, respectively. In 5 $\ell$ jar fermenter (medium 3 $\ell$), optimal air flow, agitation speed and culture time for the antibiotic(s) production were 2 vvm, 200 rpm and 48hrs, respectively. Under the optimal conditions in 5 $\ell$ jar fermenter, tell growth and antifungal activity were 2.06 $\times$ 10$^{8}$ cfu/$m\ell$ (30g-DCW/ $\ell$) and 13.4 mm, respectively. Under the culture conditions of air flow (30 vvm) and agitation speed (200 rpm) at 32$^{\circ}C$ for 10 days in 2,000 $\ell$ large scale culture (medium 1,800 $\ell$, pH 8.0), cell growth and antifungal activity were 0.81$\times$10$^{8}$ cfu/$m\ell$ (12g-DCW/ $\ell$) and 8.6 mm, respectively.

• Journal of agriculture & life science
• /
• v.46 no.2
• /
• pp.163-168
• /
• 2012

The objective of this study is to quantify the levels of ammonia and hydrogen sulfide inmechanically ventilated slurry-pit swine house according to pig's growth stage and seasonal condition. Mean concentrations of ammonia and hydrogen sulfide in the housing room of gestation/farrowing pigs were 5.60 (${\pm}2.48$) ppm and 178.4 (${\pm}204.8$) ppb in spring, 2.51 (${\pm}3.08$) ppm and 86.6 (${\pm}112.5$) ppb in summer, 4.96 (${\pm}2.84$) ppm and 182.3 (${\pm}242.6$) ppb in autumn, and 6.82 (${\pm}3.42$) ppm and 206.3 (${\pm}356.8$) ppb in winter, respectively. Mean concentrations of ammonia and hydrogen sulfide in the housing room of nursery pigs were 7.18 (${\pm}3.26$) ppm and 486.0 (${\pm}190.2$) ppb in spring, 4.23 (${\pm}2.95$) ppm and 206.4 (${\pm}186.9$) ppb in summer, 7.02 (${\pm}2.65$) ppm and 465.4 (${\pm}156.8$) ppb in autumn, and 9.25 (${\pm}3.68$) ppm and 618.4 (${\pm}298.3$) ppb in winter, respectively. Mean concentrations of ammonia and hydrogen sulfide in the housing room of growing/fattening pigs were 9.26 (${\pm}3.02$) ppm and 604.4 (${\pm}186.8$) ppb in spring, 6.78 (${\pm}3.88$) ppm and 312.5 (${\pm}215.4$) ppb in summer, 9.34 (${\pm}2.14$) ppm and 578.2 (${\pm}248.1$) ppb in autumn, and 14.65 (${\pm}3.15$) ppm and 825.3 (${\pm}316.9$) ppb in winter, respectively. As a result, mean concentrations of ammonia and hydrogen sulfide in terms of pig's growth stage were highest in growing/fattening housing room followed by nursery housing room and gestation/farrowing housing room (p<0.05). The swine house showed the highest levels of ammonia and hydrogen sulfide in winter followed by spring, autumn and summer. However, there was no significant difference of ammonia and hydrogen sulfide among seasons (p>0.05).

• Journal of Korean Public Health Nursing
• /
• v.6 no.2
• /
• pp.70-107
• /
• 1992

This study was intended to assess the need of home nursing care and analyze the effect of home nursing care, and find out the problems during the performance of home nursing care for the chronic patients among the low-income people in urban area. Data collection by interview was carried out from Nov. 1991 to Jul. 1992. The main results were as follows; 1) Total subjects for the need assessment of home nursing care were 123 households wi th 488 persons in a urban poor area. Over half of households $(57.7\%)$ was teenage family. The overall living conditions were poor and the average monthly income was 580 thousands won. $74.8\%$ of subjects was covered by medical care insurance and only $4.7\%$ was covered by public assistance. The morbidity rate was $8.2\%$ among 488 subjects and $27.5\%$ of them was not treated at all, $30\%$ was treated in utilizing pharmacies or local clinics. 2) The subjects of home nursing care were 46 with Hypertension or DM who agreeded the participation of study among registered patients at a public health center in Incheon. Home visiting was performed at intervals per one month for one year. Most of them were the elder(mean age=61 years) and long term patients(7.8 years continued). Home nursing care was effective. That is, blood pressure(including systolic and diastolic pressure) was significantly reduced (t(n=22)=2.31, P=.031, t(n=24)=4.16, P=.000 respectively) and knowledge of disease(t(N=46)=-7.63, P=.000), attitude of disease (t(N =46) = -4.92, P=.000), and self-care(t(N =46) = -4.89, P= .000) were significantly improved through home nursing care. But there was no difference in blood sugar for diabetics between the beginning and the end of visits. At the beginning of visit for home nursing care, sex$(\beta=-0414,\;t=-3.012)$ and nursing need({3=.310, t=2.164) were influencing self-care, and duration of disease$(\beta=.297,\;t=2.106)$ and nursing need $(\beta=.385, t=20417)$ were influencing blood pressure, blood sugar level. Namely, the subjects who were male and had higher nursing need showed better self-care and the longer duration of disease and the higher nursing need were relationship with the better blood pressure and blood sugar level. At the end of visit for home nursing care after one year, the blood pressure and blood sugar level was influenced by age $(\beta.320,\;t= 2.242)$, duration of disease ($(\beta.352,\;t= 2.395)$ and nursing need $(\beta=.350,\;t=2.623)$ and self-care had no influencing factor. The higher age and the longer duration of disease and the higher nursing need were relationship with the better blood pressure and blood sugar level. 3) The problems that were found out during the performance of home nursing care were the absent of useful protocols for services and the clear evaluation base, and the difficulty of teaching elders who were the major part of our subjects.

• Journal of Korean society of Dental Hygiene
• /
• v.10 no.2
• /
• pp.231-242
• /
• 2010

Objectives : The purpose of this study was to examine the relationship of oral health behavior factors to oral health promotion behavior in dental hygiene students and nonhealth-related majors, to identify factors affecting their oral health promotion behavior and ultimately to provide information on the oral health promotion of college students and behavioral factors related to that. Methods : The subjects in this study were three-year-course dental hygiene students and two-year-course nonhealth-related majors in G college located in the city of Gwangju. A self-administered survey was conducted for 11 days from September 9 to 19, 2009, and the answer sheets from 473 respondents were analyzed. To look for connections between their major and the characteristics of their oral health promotion behavior, t-test was utilized, and t-test and one-way ANOVA were carried out to find out the relationship oral health promotion behavior by general characteristics. Besides, multiple regression analysis was employed to grasp factors affecting oral health promotion behavior. Results : Among the subfactors of oral health promotion behavior, the dental hygiene students statistically significantly outdid the nonhealth-related majors in thorough post-meal toothbrushing(p<0.01), toothbrushing method(p<0.001), use of fluorine-containing dentifrice(p<0.001) and good nutrition(p<0.05). But the latter statistically significantly outperformed the former in use of fluorine-containing rinse(p<0.001) and limited sugar intake(0<0.05). Regarding variables affecting oral health promotion behavior, the age group, whether to get a dental checkup over the last year and whether to use oral hygiene supplies were identified as the variables to impact on oral health promotion behavior. Specifically, the age group(dental hygiene students) and the get a dental checkup over the last year(nonhealth-related majors) had a greater impact. Conclusions : To promote the oral health of college students, comprehensive oral health education should be provided, which covers the importance of dental checkup, recommendation for the use of oral hygiene supplies, etc. Specifically, oral health education should be offered as one of general education courses in order for nonhealth-related majors to get into sound life habits and improve their oral health promotion behavior. To make it happen, required institutional measures should be taken.

• Journal of Nutrition and Health
• /
• v.30 no.10
• /
• pp.1203-1210
• /
• 1997

This study was performed from March to June 1994 in ANsung Medical Center. We investigated serum components of 78 diabetics in the center, in addition to the nutrient intake status and anthrophometric status of 60 diabetics among them. We separated the latter into 2 groups(ⅰ) less tan 5 years of diabetic duration and ⅱ) more than 6 years of diabetic duration) and analyzed the clinical characteristics of each group. The results are as follows : \circled1 The average ages of male and female subjects were 57.1 and 58.9 years, respectively. The average diabetic duration of male subjects was 4.8 years and that of female subjects was 5.9 . The major occupational field of both male and female subjects was agriculture(33.3%, 36.1%). 50% of male and 75% of female subject's monthly incomes were less than 600, 000 won and 75% of male and 94.4% of female subjects had weekly food expense less than 40, 000 won. The nutrient intake status of the subjects was lower than RDA on the whole. There was no big differences between 2 the male groups of different diabetic duration , but calorie, protein , fat carbohydrate, phosphorus, iron and Vit B$_1$intakes of the above 6 year female group were appearently higher than those of the under 5 year female group (p<0.05). There were different intake tendencies between male and female subjects . For females. the longer the diabetic duration, the less intakes of calorie, protein , fat carbohydrate, phosphorus, iron Vit B$_1$, Vit B$_2$, and niacin were observed. Furthermore, the longer the diabetic duration in females , the more intakes of energy and nutrients, except Vit C were observed. Fasting blood glucose levels were 155mg/dl and 164mg/dl and Postprandial glucose levels were 242mg/dl and 247mg/dl for males and females respectively. Hb of both males and females , and Ht of males met the lower limit of normal status, The serum TG level was higher than normal , so that they seemed to suffer from hyperlipidemia ; their high HbAIC status indicated that the management of diabetes mellitus was poor. The total protein level of female subject was higher when compared with that of males(p<0.05). All subjects blood pressure were higher than normal , and the longer the diabetic duration, the higher was the blood pressure. The serum HDL-cholesterol levels of the above 6 year group male subjects were lower(p<0.05) than those of the under 5 year male group subjects, and the former group's systolic and diastolic blood pressures were higher than those of the latter(p<0.05). Levels of serum total protein, albumin and TG of the above 6 year female group were lower than those of the under 5 year female group(p<0.05). As the diabetic duration was extended, HDL-cholesterol and LDL-cholesterol levels showed a tendency to increase, while Hb, Ht, total protein, albumin, and A/G, which represent the nutrients state of iron and protein, were inclined to decrease. This result showed that serum lipid components went bad. To summarize the results, the longer the diabetic duration, the worse were the male subjects nutrient intake status, but those of the female subjects were good. With respect to serum components and serum lipid subjects Fe, protein, nutrition status and serum lipid component got worse as the diabetic duration was extended , implying the possibility of diabetes mellitus complication grows.

• The Korean Journal of Zoology
• /
• v.32 no.3
• /
• pp.242-251
• /
• 1989

Morphometric and genetic analyses on six populations of the two subspecies of Cobitis koreensis were performed to investigate the geographic variation and to clarify their taxonomic status. No significant differences between subspecies were found. Puan population of C. k. pumilus tends to be smaller in numbers of cross bars and dorsal spots, than those of C. k. koreensis populations with no statistical significance. Average degree of genic variation among six populations of the two subspecies was A=1.5, P =37.3%, HD=0.053, and HG=0.097. Marginal populations of Samchog (C. k. koreensis) and Puan(C. k. pumilus) were least variable: A= 1.3, P=23.8%, HD=0.0l0, HG=0.043, and A= 1.2, P=19.0%, HD=0.029, HG=0.078 respectively.The difference between HD and HG in all populations may indicate a patchness distribution between demes within each population. Genetic similarities between the two subspecies were, on the average, S=0.894, and this value correspond to the average genetic similarities among five populations of C. k. koreensis (5=0.899). Chongup population of C. k. koreensis and Puan population of C. k. pumilus was most similar genetically (5 =0.931). The results of morphometric and genetic analysis in this study indicate that C. k. pumilus is not a valid subspecies and should be considered as a population of C. koreensis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.12
• /
• pp.1705-1711
• /
• 2004

Three experiments were conducted to investigate the interaction of breed-by-chitosan supplementation on the major economic traits in broiler chickens. In experiment 1, one-day-old broiler chicks were fed ad libitum on a basal diet (CON-group) or basal diet containing chitosan at an inclusion level of 10.5 mg/bird/day (EXP-group). The EXP-group birds in experiments 2 and 3 were supplemented from 15 day-old by the same amount of chitosan used in experiment 1. In experiment 1, the mean body weight of the EXP-group chickens was significantly (p<0.05) heavier in comparison with those of CON-group birds from day 21 of the experiment. Especially in 35 day-old mean body weight, the EXP-group birds of Arbor Acres, Peterson, and Ross were significantly (p<0.05) heavier by 121.8 g, 118.5 g, and 242.8 g than the CON-group birds, respectively. However, the mean body weights in experiments 2 and 3 did not significantly differ between the CON-group birds and the EXP-group birds fed with chitosan supplementation from day 15 post birth. In the comparisons among breeds on the mean body weight at 35 day-old, the birds of Arbor Acres were significantly (p<0.05) heavier than ones of Peterson or Ross; however, there were no significant differences between Peterson and Ross or Cobb and Ross birds in overall in the experiments. The mean 15-35 d FCR of the EXP-group birds in experiment 1 were significantly (p<0.05) lower at least in two of the three breeds (Arbor Acres and Ross breeds) than that of the CON-group birds. None of the mean 15-35 d FCR in either experiment 2 or 3 showed significant differences between groups within a breed. In all three experiments, the differences amongst breeds in the mean 15-35 d FCR were not great either. Significant differences were not generally shown in the mean percentage of abdominal fat deposition between groups within a breed in overall experiments except in the Cobb breed in experiment 3. The mean percentages of abdominal fat deposition were significantly (p<0.05) lower in Ross birds than in Arbor Acres or Peterson birds and in Cobb birds than in Ross birds. By the results of the analysis of variance, the interaction of breed-by-diet (chitosan) supplementation on any of the major economic traits including mean percentage of abdominal fat deposition was not significant in overall experiments. Results of these experiments indicate that dietary supplementation with chitosan for the improvement of growth or feed conversion ratio in broilers has an efficacy when the supplementation begins from day-old.

• Journal of Korean society of Dental Hygiene
• /
• v.10 no.6
• /
• pp.1107-1119
• /
• 2010

Objectivs : The purpose of this study is to provide basic data for the use of oral hygiene devices and to improve oral health care continuously by encouraging the use of oral hygiene devices. Methods : The survey was carried out by questionnaire research targeting 395 respondents in metropolitan area in June-August 2009. The collected data is analyzed by statistics program SPSS version 12.0. 1. General characteristics of subjects are analyzed by percentage and descriptive statistics. 2. Recognition about how to use oral hygiene devices, usage of oral hygiene devices, recognition of dental brush usage, Effect after using oral hygiene devices are analyzed by mean difference test(t, F verification). 3. Effect after using oral hygiene devices, relation of oral hygiene devices usage with subjective oral health status, oral hygiene devices usage and participation in education are analyzed by Correlation, Regression analysis. Results : 1. Investigation shows that most oral health devices are rarely used except toothbrush(4.57) in case of the use of oral hygiene devices. 2. In the case of awareness of oral hygiene devices usage, almost everyone knows how to use dental brushes(MEAN 4.16) the most. And the next ones are like these-tooth picks(MEAN 3.38), dental floss(MEAN 3.28), mouth rinse(MEAN 3.24). And they don't know how to use the other oral hygiene devices usage. 3. respondents who use dental floss(${\beta}$=.238, p<.001), interdental brush(${\beta}$=.242, p<.001) and water pik(${\beta}$ =.180, p<.05) recognize that the result and effect after using them are more positive than the others in different case. 4. Respondents who use toothbrush(${\beta}$=.119), dental floss(${\beta}$=.120) and interdental brush(${\beta}$=.136). frequently evaluate their subjective oral health status positively in case of frequency of using oral hygiene devices and subjective oral health status(p<.05). 5. Respondents who use toothbrush and interdental brush properly and frequently, who are older than the others show more concern about participating in education of "How to use oral hygiene devices" than the others(p<.05). Conclusions : In conclusion, to prevent oral disease, the opportunity of participating in education about correct knowledge and proper way should be expanded. And we should contribute to oral health improvement through developing, executing and informing systematic, popular oral health education with active one.

• Journal of Korean society of Dental Hygiene
• /
• v.6 no.3
• /
• pp.229-242
• /
• 2006

This study was conducted to find out basic information on better customer satisfaction and service by examining and analyzing in what routes patients mostly have selected their dental medical organizations and how they have reacted to their dissatisfaction. The subject of study included the patients who have come to dental clinics and dental hospitals to be treated in Cheonan City, Chungnam and the researcher of this study used a survey and analyzed its gathered data using an SPSS(Statistical Package for the Social Science) WIN 11.5 Program. As analysis methods, frequency and percentage were calculated to figure out the general characteristics of its subject. In addition, a ${\chi}^2$(Chi-square) test was performed to figure out the factors that patients have selected their dental medical organizations and the following results were obtained. 1. The largest number of patients selected their dental medical organizations by hearsay and more female and married patients selected them by hearsay than other patients, and there were significant differences(p<.05). 2. The dental patients put the priority on treatment techniques in their visiting hospitals and the patients who were more than 40 years old and married patients put more emphasis on treatment techniques in their visiting hospitals than other patients and there were significant differences(p<.05). 3. The said dental patients were not satisfied with the kindness of medical staffs in their visiting medical organizations; meanwhile, the patients who were aged below 30 and had an academic background of more than university degree and were unmarried were less satisfied with the kindness of medical staffs in their visiting medical organizations than other patients and there were significant differences(p<.05). 4. In terms of improvement methods or complaints, the largest number of them said that they just ignored and endured; meanwhile, the patients who were aged below 30 and had an education of more than university degree and were unmarried were more patient of improvement methods or complaints than other patients and there were significant differences(p<.05). 5. There were more patients who have introduced their visiting medical organizations to their neighboring people than the patients who have not done it; in the meantime, the patients who were aged over 40 and were married have introduced their visiting medical organizations to their neighboring people more than other patients(p<.05).

• Journal of Korean society of Dental Hygiene
• /
• v.13 no.2
• /
• pp.181-191
• /
• 2013

Objectives : The research was performed to be used as basic data for dental hygienists' job satisfaction during clinical practice, and planning and operating effective clinical practice by analyzing stress factors in dental hygienists and the extent of stress caused by students in clinical practice. Methods : The research was aimed at dental hygienists working at college dental hospitals, dental clinic of general hospitals, dental hospitals and dental clinics located in Seoul and Gyeonggi province where clinical practices were conducted for dental hygiene students. For analysis, collected data was analyzed by using SPSS 20.0. Results : Results derived from analysis on characteristic difference by pattern of hospital were observed that there was a significant difference in job satisfaction by pattern of hospital. Results from analysis on correlation among stress factors were observed that there was correlation between behavior and attitude, interpersonal relationships, role and activity, ideal and value, and treatment fields. Results derived from analysis on the effect of stress factors on the job satisfaction were observed that role and activity (p=.002<.01), ideal and value (p=.019<.05), and treatment fields (p=.032<.05) affected the job satisfaction, while behavior and attitude (p=.386>.05), and interpersonal relationships ((p=.421>.05) did not affect the job satisfaction. A stress factor, role and activity (${\beta}$=.350) affected the job satisfaction the most, followed by ideal and value (${\beta}$=.242), and treatment fields (${\beta}$=-.185). For the explanation ability, the stress factors accounted for 20.5% of the job satisfaction. Conclusions : In conclusion, most of the dental hygienists are satisfied with students' clinical practice by pattern of hospital. Positive correlation was shown between stress factors. Behavior and attitude and ideal and value are high in stress in college dental hospitals, role and activity and treatment fields are high in stress in dental hospital, and interpersonal relationships are high in stress in dental clinics.