• Molecular & Cellular Toxicology
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• 제1권2호
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• pp.116-123
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• 2005

The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.

• 한국가금학회지
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• 제32권2호
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• pp.113-123
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• 2005

본 연구는 약병원성 조류인플루엔자 사독백신 개발을 위하여 백신 후보주(ADL0401)로서의 생물학적 특성 및 개발 백신에 대한 면역원성 및 안전성 평가를 실시하였다. 백신 후보주인 ADL0401의 병원성을 조사한 결과 폐사는 없었으며, 임상증상 및 바이러스 재분리율 양상이 국내 표준 야외주인 MS96과 상당히 유사한 생물학적 특성을 나타내어 약병원성 조류인플루엔자의 특징을 갖고 있음을 알 수 있었다. 표준야외주인 MS96과 백신후보주인 ADL0401간의 이종항원의 중화능 실험을 한 결과 r값 = 0.71로 동종항원(r값 = 1)에 비하여 다소 낮은 중화능을 나타내었지만, 두 바이러스간의 항원성엔 큰 차이가 없음을 알 수 있었다. 불활화 능력실험으로 $0.1\%$ Formalin을 $37^{\circ}C$ 조건에서 처리하였을 때 가장 효과적인 결과를 가져왔으며, MS96은 2시간, ADL0401은 1시간만에 다소의 역가 저하는 있었으나 빠르게 불활화가 이루어짐을 확인하였다. 개발 백신의 면역원성 및 안전성 실험 결과 ISA 70 adjuvant 백신 접종시 백신 접종 전후로 폐사율 및 임상증상은 관찰되지 않았으며, 높은 수준의 항체 역가를 형성함으로써 가장 면역원성이 우수한 것으로 확인되었다.

• 아시안잔디학회지
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• 제23권1호
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• pp.123-132
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• 2009

본 연구는 2007년 늦가을에서 2008년 초봄까지 골프코스 그린에서 스키 슬로프로 전환하기 전, 적설 및 제설 시기를 다르게 실시한 4개 시험구에 메틸렌 유레아(MU), 복합 화학비료(CF), 입상 휴믹산 비료(HM), 동물성 유기질 비료(NS)를 처리하여 늦가을 시비가 이듬해 봄철 bentgrass의 greenup에 미치는 영향을 분석하였다. 적설 10일전에 MU와 입상 휴믹산 비료인 입상 휴믹산 비료가 이듬해 봄에 전체적으로 가장 빠른 시각적 품질 상태와 녹색도를 보였다. 공시 비료 중 인산($P_2O_5$)함량이 가장 높은 CF는 봄철 뿌리 신장에 가장효과적이어서 골프코스로 전환 시점(4월 3일)에는 뿌리길이가 평균 13.0cm로 측정되었다. 하지만 CF처리 후 바로 적설된 시험구에서 시각적 품질과 녹색 도에서 가장 낮은 결과를 보여 최소 적설 10일 전에 시비하여야 적설에 의해 용해되지 않았던 잔류 비해를 피할 수 있는 것으로 나타났다. 동물성 유기질 비료의 시비는 적설처리하지 않았던 시험구에서는 잎의 건물 중이 가장 높았으나 무시비구와 봄철 녹색도 및 시각적 품질평가에서 차이를 보이지 않았다. 따라서 골프코스에서 스키슬로프로 전환하기 위한 적설 시기와 골프 코스로의 재 전환을 위한 적절한 늦가을 시비관리 및 제설 시기는 이듬해 봄철 bentgrass의 greenup과 잔디 생육을 위한 중요한 요소로 분석 되었다.

• 대한인간공학회지
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• 제36권3호
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• pp.169-181
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• 2017

Objective: The aim of this study was to explore the influence of outdoor weather conditions on subjective responses during physical activity. Background: The largest difference between indoor and outdoor conditions is the existence of the sun. The heat load from the sun has an influence on the heat gain of the human body and the intense degree of solar radiation affected thermal comfort. Method: Thirty eight people were exposed to a range of climatic conditions in the UK. Weather in England does not have extremely hot and cold temperature, and the current study was conducted under warm (summer and autumn) and cool (spring and summer) climates. Measurements of the climate included air temperature, radiant temperature (including solar load), humidity and wind around the subjects. Subjective responses were taken and physiological measurements included internal body temperature, heart rate and sweat loss. Results: This study was conducted under four kinds of environmental conditions and the environmental measurement was performed in September, December, March, and June. The values for sensation, comfort, preference, and pleasantness about four conditions were from 'neutral' to 'warm', from 'not uncomfortable' to 'slightly comfortable', from 'slightly cooler' to 'slightly warmer', and from 'neither pleasant nor unpleasant' and 'slightly unpleasant', respectively. All subjective responses showed differences depending on air temperature and wind speed, and had correlations with air temperature and wind speed (p<0.05). However, subjective responses showed no differences depending on the radiant temperature. The combined effects of environmental parameters were showed on some subjective responses. The combined effects of air temperature and radiant temperature on thermal sensation and pleasantness were significant. The combined effects of metabolic rate with air temperature, wind speed and solar radiation respectively have influences on some subjective responses. In the case of the relationships among subjective responses, thermal sensation had significant correlations with all subjective responses. The largest relationship was shown between preference and thermal sensation but acceptance showed the lowest relationship with the other subjective responses. Conclusion: The ranges of air temperature, radiant temperature, wind speed and solar radiation were $6.7^{\circ}C$ to $24.7^{\circ}C$, $17.9^{\circ}C$ to $56.6^{\circ}C$, $0.84ms^{-1}$ to $2.4ms^{-1}$, and $123Wm^{-2}$ to $876Wm^{-2}$ respectively. Each of air temperature and wind speed had significant relationships with subjective responses. The combined effects of environmental parameters on subjective responses were shown. Each radiant temperature and solar radiation did not show any relationships with subjective responses but the combinations of each radiant temperature and solar radiation with other environmental parameters had influences on subjective responses. The combinations of metabolic rate with air temperature, wind speed and solar radiation respectively have influences on subjective responses although metabolic rate alone hardly made influences on them. There were also significant relationships among subjective responses, and pleasantness generally showed relatively high relationships with comfort, preference, acceptance and satisfaction. Application: Subjective responses might be utilized to predict thermal stress of human and the application products reflecting human subjective responses might apply to the different fields such as fashion technology, wearable devices, and environmental design considering human's response etc.

• 한국식품저장유통학회지
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• 제12권2호
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• pp.123-129
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• 2005

일반인의 식사대용이나 노약자의 영양공급용 기능성 영양균형선식을 제조하고 그의 품질특성을 조사하였다. 영양학적 성분은 탄수화물의 함량이 가장 높았으며, 단백질함량은 일반선식이 $21.96\%$로 영양균형선식의 $16.76\%$ 보다 상대적으로 높은 함량을 나타내었다. 주된 유리당으로 sucrose 함량은 영양균형선식이 226.91 mg/100 g으로 일반 선식 74.82 mg/100 g 보다 높은 함유량이었다. 주요 유기산으로는 malic acid의 경우는 일반선식과 영양균형선식에 각각 15.41 mg/100 g과 36.76 mg/100 g로 높은 함량이었다. 주요 유리아미노산으로 영양균형선식에는 serine이 180.31 mg/100 g으로 일반선식의 serine 함량보다 약 2배정도로 높았다. 주요지방산으로 Linoleic acid의 함량은 일반선식에 $38.16\%$와 영양균형선식에 $29.30\%$로 높은 함유량을 보였다. 주된 무기질로 P의 함량은 일반선식에는 641 mg/100 g으로 높은 함량이었으나 영양균형선식에서는 457.64 mg/100 g 함량 수준으로 낮았고, 또한 Ca함량은 일반선식에 290.08 mg/100 g과 영양균형선식에 234.78 mg/100 g로 높은 함량을 보였다. 항산화활성의 경우는 영양균형선식 개발제품은 BHA 200 ppm과 BHT 200 ppm에 비해 약$29\%$ 정도의 항산화력을, $\alpha-tocopherol$ 400 ppm에 비해 약 $40\%$ 정도의 항산화력을 가지는 것으로 나타났다. 관능평가에서는 영양균형선식이 외관적 색, 고소한 맛, 향미, 씹힘성 및 전체적인 기호도 등에서 높은 관능점수를 얻었다.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.135-139
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• 2015

A total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including Apodemus agrarius (n = 1,400), Microtus fortis (167), Crocidura lasiura (91), Mus musculus (32), Myodes (= Eothenomys) regulus (9), Micromys minutus (6), and Tscherskia (= Cricetulus) triton (3), were live-trapped at US/Republic of Korea (ROK) military training sites near the demilitarized zone (DMZ) of Paju, Pocheon, and Yeoncheon, Gyeonggi Province from December 2004 to December 2009. Small mammals were examined for their intestinal nematodes by necropsy. A total of 1,617 rodents (100%) and 91 (100%) soricomorphs were infected with at least 1 nematode species, including Nippostrongylus brasiliensis, Heligmosomoides polygyrus, Syphacia obvelata, Heterakis spumosa, Protospirura muris, Capillaria spp., Trichuris muris, Rictularia affinis, and an unidentified species. N. brasiliensis was the most common species infecting small mammals (1,060; 62.1%) followed by H. polygyrus (617; 36.1%), S. obvelata (370; 21.7%), H. spumosa (314; 18.4%), P. muris (123; 7.2%), and Capillaria spp. (59; 3.5%). Low infection rates (0.1-0.8%) were observed for T. muris, R. affinis, and an unidentified species. The number of recovered worms was highest for N. brasiliensis (21,623 worms; mean 20.4 worms/infected specimen) followed by S. obvelata (9,235; 25.0 worms), H. polygyrus (4,122; 6.7 worms), and H. spumosa (1,160; 3.7 worms). A. agrarius demonstrated the highest prevalence for N. brasiliensis (70.9%), followed by M. minutus (50.0%), T. triton (33.3%), M. fortis (28.1%), M. musculus (15.6%), C. lasiura (13.2%), and M. regulus (0%). This is the first report of nematode infections in small mammals captured near the DMZ in ROK.

• 한국식품조리과학회지
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• 제30권4호
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• pp.463-471
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• 2014

Yetgangjeong is a traditional Korean rice cookie which used rice syrup (Chocheong) to bind fried rice grains. Brown waxy rice and binders prepared using different sugar types and end heating temperatures were assessed to improve the textural properties and functionality and to promote global consumption. Binder was made by mixing different ratios of starch syrup, rice syrup, honey, sugar, and trehalose, and the binder mixture was heated up to a temperature of $100^{\circ}C$ or $105^{\circ}C$. Specific electrical conductivity of binder containing ST (starch syrup and trehalose) showed the lowest values (9.51 and 9.77), and binders containing the rice syrup showed increased values for specific electrical conductivity. The fructose content was higher in the binder mixture containing C (starch syrup and sugar) than in the binder mixture containing ST, but it did not affect the end temperature. Viscosity of the binder was 123.90-175.20 cP and the binder showed higher viscosity at the end heating temperature (EHT) of $100^{\circ}C$ compared at EHT of $105^{\circ}C$. The fracturability of brown rice yetgangjeong prepared using different sugar types was higher at EHT of $100^{\circ}C$ than at that of $105^{\circ}C$ and it was reduced with an increase in the rice syrup content. However, the hardness of yetgangjeong was lower at $100^{\circ}C$, unlike fracturability. With respect to sensory evaluation, its moistness and stickiness decreased, but the crunchiness increased with addition of trehalose instead of sucrose. The overall eating quality score of yetgangjeong was higher at EHT of $105^{\circ}C$ than at that of $100^{\circ}C$ in every experiment and the highest score was found for SHT (starch syrup, honey and trehalose) with 20% rice syrup at EHT of $105^{\circ}C$ (20%RSHT105 (starch syrup, honey, and trehalose containing 20% rice syrup)).

• 대한핵의학회지
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• 제14권1호
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• pp.1-8
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• 1980

Serum TSH levels were measured by radioimmunoassay before and after intravenous administration of synthetic thyrotropin-releasing hormone (TRH) to 15 normal subjects and 55 patients with primary thyroid disease (14 patients with euthyroidism, 24 patients with thyrotoxicosis and 17 patients with hypothyroidism) to evaluate pituitary TSH reserve and its diagnostic availability. The observed results were as follows. 1. In normal subjects, serum TSH responses to synthetic TRH were $3.2{\pm}1.0$ at 0min (baseline TSH level), $8.0{\pm}4.0$ at 10min, $11.7{\pm}5.0$ at 20min, $13.7{\pm}7.1$ at 30min, $9.7{\pm}5.0$ at 60min., $5.2{\pm}2.0$ at 120min. and $3.6{\pm}0.4{\mu}U/ml$ at 180 min. Serum TSH peaked at $20{\sim}30$ minutes and returned nearly to baseline at 180minutes. 2. In euthyroid group, serum TSH responses to synthetic TRH were $3.3{\pm}1.6$ at 0min, $8.6{\pm}8.0$ at 10min, $10.9{\pm}8.5$ at 20min, $12.5{\pm}8.4$ at 30min, $9.0{\pm}5.9$ at 60min, $5.6{\pm}2.6$ at 120min and $3.5{\pm}1.3{\mu}U/ml$ at 180min. No significant difference revealed between euthyroid group and normal subjects (p>0.05). 3. In hyperthyroid group, serum TSH responses to synthetic TRH were $1.5{\pm}0.6$ at 0min, $2.2{\pm}0.8$ at 10min., $2.3{\pm}1.0$ at 20min., $2.4{\pm}1.5$ at 30min., $2.1{\pm}1.1$ at 60min., $1.9{\pm}0.2$ at 120min. and $1.5{\pm}0.8{\mu}U/ml$ at 180min., No response to TRH showed. 4. In hypothyroid group, mean values of serum TSH response to synthetic TRH were 42.0 at 0min., 60.6 at 10min., 124.8 at 20min., 123.0 at 30min., 101.6 at 60min., 64.3 at 120min. and $15.5{\mu}U/ml$ at 180 min., Patients with primary hypothyroidism showed an exaggerated TSH response to synthetic TRH despite their high basal TSH. 5. Side effects attending synthetic TRH administration were transient nausea (59.0%), desire to micturate (59.0%), feeling of flushing(19.7%), dizziness (45.9%), metallic taste (9.8%) and headache (19.7%). Any side effect didn't show in 16.4%. These symptoms began almost immediately after TRH intravenous injection and lasted several minutes, and not related to dose or response in the person experiencing it.

• 성인간호학회지
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• 제13권1호
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• pp.123-135
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• 2001

The purpose of this study is to analyze the structural pattern of nurses' pain management with patients who experience pain. As a research method, the Q methodology, which is useful for an objective view of a highly abstract concept, was applied. The data collecting activity of this study was from August, 21st, 2000 to November, 24th 2000. The Q-population, the previous thesis and a literature review were done. Questions about pain management by the nurse on the patients, caregivers, nursing students, doctors, nurses, and others were asked in documentary work and in-depth interviews. In all, 223 units of the Q-population were formed, and the last 35 units of the Q-population were extracted. The data on the P-sample was collected from 41 nurses who worked in the medical and surgical units of a hospital that belonges to C university in Seoul. The research results were constituted in 3 types. Type I was the 'pattern of judging by objectivity'. The statement on which most of the people highly agreed for those patterns was shown by 'If patients said that they are suffering from pain, we sufficiently performed an assessment about the etiology, location, duration and degree'. For type I, the same pain was found in different locations according to the patients, so the etiology of the pain should be identified first place. Since ways of coping are different according to pain etiology, it was thought that it is important to assess sufficiently the pain etiology, location, duration, and degree. Therefore, when patients complain of pain, the pain etiology should be identified and assessed; according to the result, pain management should be performed systematically. Type II was the 'pattern of accepting by subjectivity'. The statement on which most of the people highly agreed for those patterns was shown as 'If patients said that they are suffering from pain, the medical treatment should be performed rapidly and speedily.' For type II, when the patient complains of pain, treatment should be performed quickly in order to prevent the condition getting worse, and it is thought that activity is a reasonable duty. Further, by trying to show empathy after pain is admitted and by understanding and coping rapidly with the pain of patients, an attitude which matched the altruistic morals of nurses is being shown. Type III was the 'pattern of worrying about', and the statement on which most of the people highly agreed for those patterns was shown is 'When there is a pain, to help patients to tolerate the pain to the highest degree.' In type III, the pain is a subjective expression, so there is a difference according to every individual. Therefore, actually if there is no measurement of pain, it could be exaggerated so nurses should help patients to tolerate it to the utmost. Even if there is a way to remove pain without an analgesic drug, nurses were reluctant to perform pain management as they possibly could. Through these research results, pain management of nurses was classified in 3 types, and structural characteristics in each type were discovered. Based upon the characteristics according to the type, an individualized pain management intervention strategy should be established and the follow up work performed.

• 한국안광학회지
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• 제11권4호
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• pp.337-344
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• 2006

시력교정(라식, 라섹, 엑시머)수술이 근시환자의 삶의 질에 미치는 영향에 대하 기존 연구들이 있었으나, 수술을 받은 환자들을 대상으로 수술 전후의 삶의 질 변화를 조사하는데 그쳤다. 본 연구에서는 시력교정 수술을 받은 환자들과 수술을 받지 않고 안경이나 콘택트렌즈를 계속 착용하는 사람들의 삶의 질을 비교 분석하였다. 근시환자 470명에 시력관련 삶의 질을 평가하기 위하여 QIRC(The Quality of Life Impact of Refractive Correction)설문지를 한국어 번역판을 개발하여 사용하였다. 공분산분석(ANCOVA)을 이용하여 주요혼란변수를 보정 한 후 안경 및 콘택트렌즈 착용 자, 시력교정수술을 받은 자. 그리고 수술 후 안경을 재 착용하는 자 등 세집단간의 삶의 질을 비교한 결과 시력교정수술을 받은 군은 안경이나 콘택트렌즈로 교정을 받은 군에 비해서 삶의 질이 유의하게 높았다. 그러나 수술 후 근시로 이행하여 안경을 재 착용하게된 군의 삶의 질은 수술을 하지 않은 군보다 더 삶의 질이 낮은 것으로 조사되었다. 시력교정수술이 근시환자의 삶의 질에 미치는 영향을 평가하기 위해서는 시력교정수술 후에도 다시 안경을 착용해야 하는 경우 수술 전보다 삶의 질이 더 낮아질 수 있다는 점을 고려하여야 할 것으로 보인다.