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Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice

  • Kim, Dongwook;Kim, Yuan H. Brad;Ham, Jun-Sang;Lee, Sung Ki;Jang, Aera
    • Food Science of Animal Resources
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    • v.40 no.2
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    • pp.183-196
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    • 2020
  • The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 20-25 g were randomly assigned to seven groups: Control (CON); scopolamine (SCO, 1 mg/kg B.W., intraperitoneally (i.p.); tetrahydroaminoacridine 10 [THA 10, tacrine; 10 mg/kg B.W. per oral (p.o.) with SCO (i.p.)]; PSW 10 (10 mg/kg B.W. (p.o.) with SCO (i.p.); PSW 40 (40 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 100 (100 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 400 (400 mg/kg B.W. (p.o.) with SCO (i.p.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 μM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly affect on physical performance of mice. LPSW 400 significantly increased spontaneous alternation, reaching the level observed for THA and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine (ACh) content and decreased ACh esterase activity (p<0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

SIMPLE VALUATION IDEALS OF ORDER TWO IN 2-DIMENSIONAL REGULAR LOCAL RINGS

  • Hong, Joo-Youn;Lee, Hei-Sook;Noh, Sun-Sook
    • Communications of the Korean Mathematical Society
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    • v.20 no.3
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    • pp.427-436
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    • 2005
  • Let (R, m) be a 2-dimensional regular local ring with algebraically closed residue field R/m. Let K be the quotient field of R and v be a prime divisor of R, i.e., a valuation of K which is birationally dominating R and residually transcendental over R. Zariski showed that there are finitely many simple v-ideals $m=P_0\;{\supset}\;P_1\;{\supset}\;{\cdotS}\;{\supset}\;P_t=P$ and all the other v-ideals are uniquely factored into a product of those simple ones. It then was also shown by Lipman that the predecessor of the smallest simple v-ideal P is either simple (P is free) or the product of two simple v-ideals (P is satellite), that the sequence of v-ideals between the maximal ideal and the smallest simple v-ideal P is saturated, and that the v-value of the maximal ideal is the m-adic order of P. Let m = (x, y) and denote the v-value difference |v(x) - v(y)| by $n_v$. In this paper, if the m-adic order of P is 2, we show that $O(P_i)\;=\;1\;for\;1\;{\leq}\;i\; {\leq}\;{\lceil}\;{\frac{b+1}{2}}{\rceil}\;and\;O(P_i)\;=2\;for\;{\lceil}\;\frac{b+3}{2}\rceil\;{\leq}\;i\;\leq\;t,\;where\;b=n_v$. We also show that $n_w\;=\;n_v$ when w is the prime divisor associated to a simple v-ideal $Q\;{\supset}\;P$ of order 2 and that w(R) = v(R) as well.

Comparison of the Ligating Ability of Anonic Transition Metal Complex $(Mn(CO)_{5}{^-})$, Transition Metal Hydrides $(HCr(CO)_{5}{^-},\;HW(CO)_{5}{^-},\;cis-HW(CO)_{4}P(OMe)_{3}{^-},\;HFe(CO)_{4}{^-},\;trans-\;HFe(CO)_{3}P(OMe)_{3}{^-})$, and Traditional Ligands $(Br^-,\;P(C_{6}H_{5})_{3})\;to\;M(CO)_{5}{^0}$ (M = Cr, W)

  • Park, Yong K;Han, In S;Marcetta Y. Darensbourg
    • Bulletin of the Korean Chemical Society
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    • v.15 no.6
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    • pp.436-442
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    • 1994
  • Heterobimetallic complexes have a donor-accepter metal-metal bond in which two electrons from the electron-rich metal moiety are donated to the other electron-deficient one. Based on the competition reactions, Cotton-Kraihanzel force constants, ν(CO)IR band resolution and the relative nucleophilicity comparison of the donor ligands, the following relative ligating ability of the donor ligands toward $M(CO)_5$ (M=Cr, W) is assessed: cis-HW$(CO)_4P(OMe)_3^-$, $HW(CO)_5^-$ > $HCr(CO)_5^-$-$Br^-$ > trans-HFe$(CO)_3P(OMe)_3^-$ > $Mn(CO)_5^-$ > $HFe(CO)_4^-$ > PP$h_3$

EFFECT OF GRASS FILTER STRIPS ON REDUCING $PO_4$-P LOSS IN RUNOFF FROM FORAGE CROPLAND

  • Jung, M.W.;Jo, N.C.;Yoon, S.H.;Kim, W.H.;Kim, K.Y.;Sung, S.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.169-173
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    • 2011
  • The performance of grass filter strips (GFS) in abating $PO_4$-P concentrations from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage croplands with rye-corn double cropping system applied with chemical fertilizer and livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated Main plots consisted of the length of GFS, such as 0m, 5m, 10m and 15m. Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). Concentrations of PO4-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10m and 15m reduced $PO_4$-P concentrations significantly compared to that with 0m and 5m (p<0.05). The results from this study suggest that GFS improved the removal and trapping $PO_4$-P from forage croplands.

Optimization of Betacyanin Production by Red Beet (Beta vulgaris L.) Hairy Root Cultures. (Red Beet의 모상근 배양을 이용한 천연색소인 Betacyanin 생산의 최적화)

  • Kim, Sun-Hee;Kim, Sung-Hoon;Lee, Jo-No;An, Sang-Wook;Kim, Kwang-Soo;Hwnag, Baik;Lee, Hyeong-Yong
    • Microbiology and Biotechnology Letters
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    • v.26 no.5
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    • pp.435-441
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    • 1998
  • Optimal conditions for the production of natural color, betacyanin were investigated by varying light intensity, C/N ratio, concentrations of phosphate and kinds of elicitors. Batch cultivation was employed to characterize cell growth and betacyanin production of 32 days. The maximum specific growth rate, ${\mu}$$\sub$max/, was 0.3 (1/day) for batch cultivation. The maximum specific production rate, q$\^$max/$\sub$p/, was enhanced 0.11 (mg/g-cell/day) at 3 klux. A light intensity of 3 klux was shown to the best for both cell growth and betacyanin production. The maximum specific production rate was 0.125 (mg/g-cell/day) at 0.242 (1/day), the maximum specific growth rate. The dependence of specific growth rate on the light lintensity is fit to the photoinhibition model. The correlation between ${\mu}$ and q$\sub$p/ showed that the product formation parameters, ${\alpha}$ and ${\beta}$$\sub$p/ were 0.3756 (mg/cell) and 0.001 (mg/g-cell/day), respectively. The betacyanin production was partially cell growth related process, which is different from the production of a typical product in plant cell cultures. In C/N ratio experiment, high carbon concentration, 42.1 (w/w) improved cell growth rate while lower concentration, 31.6 (w/w) increased the betacyanin production rate. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.26 (1/day) and 0.075 (mg/g-cell/day), respectively. Beta vulgaris L. cells under 1.25 mM phosphate concentration produced 10.15 mg/L betacyanin with 13.46 (g-dry wt./L) of maximum cell density. The production of betacyanin was elongated by adding 0.1 ${\mu}$M of kinetin. This also increased the cell growth. Optimum culture conditions of light intensity, C/N, phosphate concentration were obtained as 5.5 klux, 27 (w/w), 1.25 mM, respectively by the response surface methodology. The maximum cell density, X$\sub$max/, and maximum production, P$\sub$max/, in optimized conditions were 16 (g-dry wt./L), 12.5 (mg/L) which were higher than 8 (g-dry wt./L), 4.48 (mg/L) in normal conditions. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.376 (1/day) and 0.134 (mg/g-cell/day) at the optimal condition. The overall results may be useful in scaling up hairy root cell culture system for commercial production of betacyanin.

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Electrical contact property and microstructure of Ni-P alloy added W-Cu contact materials (Ni-P합금 첨가한 W-Cu접점의 전기접점특성과 미세조직)

  • 김태형;배광욱;이재성
    • Electrical & Electronic Materials
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    • v.3 no.4
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    • pp.325-331
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    • 1990
  • 본 연구는 텅스텐 소결에 저온 활성제로 작용하는 Ni-P 공정합금을 미량첨가하여 제조공정의 간단화를 통한 새로운 W-Cu계 전기접점제조를 목표로 하였다. 이를 위해 1회 용침공정을 통해 제조한 W(Ni-P)-Cu 합금에 대한 전기접점특성을 조사하여 접점의 미세구조 관점에서 논의하였다. Ni-P 합금첨가한 접점은 기존의 순수 W-Cu 합금에 비해 낮은 접촉저항 및 낮은 아크소모를 나타내는 우수한 접점성능을 보여주었다. 이것은 Ni-P합금이 Cu용침이 개시되기전 짧은 승온단계에서 분말간의 강한 결합과 Cu용침에 유리한 기공통로를 갖는 W 분말 골격체의 형성을 유도하기 때문인 것으로 판단된다.

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ON F-STRUCTURE MANIFOLD

  • Upadhyay, M.D.;Das, Lovejoy S.K.
    • Kyungpook Mathematical Journal
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    • v.18 no.2
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    • pp.277-283
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    • 1978
  • The first part of this paper is devoted to the study of F-structure satisfying: $F^K+(-)^{K+1}F=0$ and $F^W+(-)^{W+1}F{\neq}0$, for 1$${\geq_-}3$$) has been considered. In the later part some structures involving Lie-derivatives. exterior and co-derivatives have been studied.

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Solubilization of BSA into AOT Reverse Micelles Using the Phase-Transfer Method: Effects of pH and Salts (상 접촉법을 이용한 BSA의 AOT 역미셀으로 가용화: pH와 염의 영향)

  • 노선균;강춘형
    • KSBB Journal
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    • v.16 no.1
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    • pp.48-53
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    • 2001
  • Bovine serum albumin(BSA) was solubilized into the reverse micellar phase consisting of sodium bis(2-ethylhexyl) sulfosuccinate(AOT) and isooctane using the phase transfer method. Of particular interest in this study were the effects of pH and the added salt type and concentration on the solubilization efficiency. When univalent or divalent salts such as KCl, NaCl, $MgCl_2$, or $CaCl_2$ were added to the aqueous phase at a concentration of 0.1 M, maximum solubilization efficiency was attained at a pH ranging from 5 to 7, depending on the added salt type. Increased salt concentration up to 1 M resulted in an increased solubilization efficiency for $CaCl_2$ and NaCl, while the addition of $MgCl_2$ beyond 0.1 M showed an anomalous trend. Further, it was noteworthy that too a large extent the protein precipitated in the interface between the organic and aqueous phases at lower pHs and lower salt concentrations. The size of the reverse micelle water pool was estimated by measuring the molar ratio of the surfactant to the water, $W_0$. Irrespective of pH in the aqueous phase, the resulting value of $W_0$ was almost constant, eg., 20 for $MgCl_2$ . However, the value of $W_0$ decreased with increased salt concentration in the cases of KCl and $CaCl_2$.

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Fabrication Process and Power Generation Characteristics of Thermoelectric Thin Film Devices for Micro Energy Harvesting (미세 열에너지 하비스팅용 열전박막소자의 형성공정 및 발전특성)

  • Oh, Tae Sung
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.3
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    • pp.67-74
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    • 2018
  • Thermoelectric thin film devices of the in-plane configuration consisting of 8 pairs of n-type $Bi_2Te_3$ and p-type $Sb_2Te_3$ legs were processed on Si submounts by electrodeposition. The thermoelectric generation characteristics of the thin film devices were investigated with respect to the apparent temperature difference ${\Delta}T$ caused by LED lighting as well as the change of the leg thickness. When ${\Delta}T$ was 7.4 K, the open circuit voltages of 6.1 mV, 7.4 mV, and 11.8 mV and the maximum output powers of 6.6 nW, 12.8 nW, and 41.9 nW were measured for the devices with the thermoelectric legs of which thickness were $2.5{\mu}m$, $5{\mu}m$, and $10{\mu}m$, respectively.