• Title/Summary/Keyword: P.I.

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Changes in Isozyme Patterns of Peroxidase and Esterase during Regeneration of Pepper (Capsicum annuum L.) (고추 (Capsicum annuum L.)의 재분화에 따른 Peroxidase와 Esterase 동위효소의 양상 변화)

  • 정현숙
    • Journal of Plant Biology
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    • v.35 no.1
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    • pp.9-15
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    • 1992
  • The plantlet was regenerated on MS medium containing BAP (2 mg/I) and 1M (1 mg/I) from leaf discs of pepper after 3 weeks of culture. And then, we investigated the activity of peroxidase and esterase and the pattern of their isozymes from leaf, stem and root in order to observe physiological and biochemical changes on the developemental stage, respectively. The peroxidase was expressed with tissue specificity because peroxidase activity according to the developemental stage of the tissue was not only highest in the leaf of the pepper at 10 days after it germinated but also 2 new bands of its isozyme were found in pI 7.2 and pI 5.2. However, a new pI 3.4 band was found in the leaf and root of the pepper after 14 days of germination, and in the stem was found out pI 5.2 band. As regeneration of leaf dises was progressed, its peroxiase activity was increased about 80% more than that of control after 14 days of culture and new pI 3.2 and 6.5 bands of it isozyme were found. The results suggested that peroxidase would be connected with regeneration of pepper. Also, esterase activity was increased about 50% more than that of control after 14 days of culture, the pattern of esterase isozyme was shown to be 3 cathodic bands and 1 anodic band after 7 days of culture.ulture.

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Advanced C.I.P Method to Use the Steel-Casing with Inner Joint (조인트 부착 강관 케이싱을 이용한 개량형 C.I.P 공법)

  • Jang, Seoyong;Choi, Jaesoon;Song, Byungwoong;Choi, Yoonyoung;Yoon, Joongsan
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.2
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    • pp.95-102
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    • 2012
  • In this study, practical verifications for an advanced C.I.P(Cast in Place Pile) construction method were carried out. The structural characteristics of the method is to attach an angular joint in the steel-casing. This joint plays an important role in boring vertically, connected pile to pile, and protects the permeation of the ground water. For verifications, experimental research and numerical analysis were performed. In the experimental research, two model-tests were set up with the real scale steel-casing. One is to examine the leakage in the joint of piles and the other is to compare earth pressures in the front and the joint, respectively. In addition, 3 point bending test and compressive loading test were carried out and numerical analysis was performed to simulate the loading test. As a result of model-tests, the leakage in the pile joints was not shown up to 300 KPa of water pressure and stress concentration in the joint is out of the question. From the results of bending and compressive test, it was found that the new advanced C.I.P method is more convenient and superior than the conventional method.

The Study of Distribution Changing and Community Characteristics of Daphniphyllum macropodum (National Monument No. 91) in Naejangsan National Park (내장산국립공원 굴거리나무군락의 군집특성 및 분포 변화 연구)

  • Shin, Jin-Ho;Jeon, Yong-Sam;Son, Ji-Won
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.19 no.3
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    • pp.45-57
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    • 2016
  • This study was focused on the northernmost limit of the distribution changing of Daphniphyllum macropodum and studied characteristic of plant communities of natural monument No. 91 in Najangsan national park, Korea. The results of this study were as follows : 1. The highest importance percentage(I.P.) value at tree layer in Quercus. mongolica community was Q. mongolica, 37.8%. But the highest value of mean importance percentage(M.I.P.) was D. macropodum, 32.8%. 2. The highest I.P. value at tree layer in Carpinus laxiflora community was C. laxiflora, 47.4%. The highest M.I.P. value was C. laxiflora, 28.8% and M.I.P. value of D. macropodum was 24.0%. 3. The highest I.P. value at tree layer in D. macropodum community was D. macropodum, 55.6%. C. laxiflora and Q. mogolica I.P. value was 14.8% and 6.8%, respectively. The highest M.I.P. value was D. macropodum, 47.9%. Sapium japonicum and C. laxiflora M.I.P. value was 11.4% and 10.7%, respectively. 4. The highest I.P. value at tree layer in Quercus variabilis community was C. laxiflora, 20.8%. Q. variabilis and Acer pseudosieboldianum I.P. value was 15.3% and 12.5%, respectively. The highest M.I.P. value was D. macropodum, 21.5%. It needs to the continuous monitoring of vegetation and importance percentage change in tree layer and subtree layer of D. macropodum. The researching results of D. macropodum distribution, the distribution range of D. macropodum showed expanded more than range of Lim and Oh' result(1999). In other words, it was distributed low density level between Najangsa temple and Byeokryeonam, and distributed in upper Keumsun waterfall and Wonjeok upper valley. D. macropodum was appeared on hiking trails around from Wonjeokam to Bulchulbong. Especially, it was found in this study that D. macropodum was distributed on Seoraebong's north which placed outside area of Naejangsa region. In this study, it was considered that distribution range of D. macropodum was expanded. Also, it is expected to be used as a result of the field study of changing distribution study of broad leaved evergreen forest due to global warming.

ON THE RATIO OF TATE-SHAFAREVICH GROUPS OVER CYCLIC EXTENSIONS OF ORDER p2

  • Yu, Hoseog
    • Honam Mathematical Journal
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    • v.36 no.2
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    • pp.417-424
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    • 2014
  • Let A be an abelian variety defined over a number field K and p be a prime. Define ${\varphi}_i=(x^{p^i}-1)/(x^{p^{i-1}}-1)$. Let $A_{{\varphi}i}$ be the abelian variety defined over K associated to the polynomial ${\varphi}i$ and let Ш($A_{{\varphi}i}$) denote the Tate-Shafarevich groups of $A_{{\varphi}i}$ over K. In this paper assuming Ш(A/F) is finite, we compute [Ш($A_{{\varphi}1}$)][Ш($A_{{\varphi}2}$)]/[Ш($A_{{\varphi}1{\varphi}2}$)] in terms of K-rational points of $A_{{\varphi}i}$, $A_{{\varphi}1{\varphi}2}$ and their dual varieties, where [X] is the order of a finite abelian group X.

Photofragment Translational Spectroscopy of CH₂I₂ at 304 nm: Polarization Dependence and Energy Partitioning

  • 정광우;Temer S. Ahmadi;Mostafa A. El-Sayed
    • Bulletin of the Korean Chemical Society
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    • v.18 no.12
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    • pp.1274-1280
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    • 1997
  • The photodissociation dynamics of CH2I2 has been studied at 304 nm by state-selective photofragment translational spectroscopy. Velocity distributions, anisotropy parameters, and relative quantum yields are obtained for the ground I(2P3/2) and spin-orbit excited state I*(2P1/2) iodine atoms, which are produced from photodissociation of CH2I2 at this wavelength. These processes are found to occur via B1 ← A1 type electronic transitions. The quantum yield of I*(2P1/2) is determined to be 0.25, indicating that the formation of ground state iodine is clearly the favored dissociation channel in the 304 nm wavelength region. From the angular distribution of dissociation products, the anisotropy parameters are determined to be β(I)=0.4 for the I(2P3/2) and β(I*)=0.55 for the I*(2P1/2) which substantially differ from the limiting value of 1.13. The positive values of anisotropy parameter, however, show that the primary processes for I and I* formation channels proceed dominantly via a transition which is parallel to I-I axis. The above results are interpreted in terms of dual path formation of iodine atoms from two different excited states, i.e., a direct and an indirect dissociation via curve crossing between these states. The translational energy distributions of recoil fragments reveal that a large fraction of the available energy goes into the internal excitation of the CH2I photofragment; < Eint > /Eavl=0.80 and 0.82 for the I and I* formation channels, respectively. The quantitative analysis for the energy partitioning of available energy into the photofragments is used to compare the experimental results with the prediction of direct impulsive model for photodissociation dynamics.

Relationship between the Body Fat Mass Measured by Bioelectrical Impedance Analysis(BIA) and Dual Energy X-ray Absorptiometry(DEXA), and by the Indices of Insulin Sensitivity (생체 임피던스 방법과 이중 방사선 흡수법으로 측정한 체지방량과 인슐린감수성 지표와의 연관성)

  • Lim, In Seok;Yun, Ki Wook
    • Clinical and Experimental Pediatrics
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    • v.48 no.8
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    • pp.857-864
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    • 2005
  • Purpose : The objectives of this study was to evaluate the correlations between the indices of insulin sensitivity using fasting glucose and insulin level, and the body fat mass measured by bioelectrical impedance analysis(BIA) and dual energy X-ray absorptiometry(DEXA), and to determine the clinical usefulness of insulin sensitivity indices when obese children were followed up. Methods : In this study, 28 simple obese children and adolescents were included. Anthropometric data including body weight, height, obesity degree(OD), body mass index(BMI), and waist-to-hip ratio were collected and then body fat mass was measured by using BIA and DEXA. For metabolic data, 12 hour fasting serum glucose, insulin and lipid profiles were measured and indices for insulin sensitivity(G/I ratio, $log_{insulin}$, HOMA-IR, $log_{HOMA-IR}$, QUICKI) were calculated. Results : BMI had a higher correlation with insulin sensitivity indices than OD(G/I ratio, -0.463 vs -0.209; $log_{insulin}$, 0.417 vs 0.196; HOMA-IR, 0.301 vs 0.238; $log_{HOMA-IR}$, 0.403 vs 0.198; QUICKI, -0.451 vs -0.224). But OD had a higher correlation with body fat mass measured by BIA and DEXA than BMI(BIA, 0.612 vs 0.316; DEXA, 0.667 vs 0.512). The G/I ratio was correlated with body fat mass in BIA(r=-0.420, P<0.05) and DEXA(r=-0.512, P<0.01), percentage of body fat(percentage of fat) in BIA(r=-0.366, P<0.05) and DEXA(r=-0.449, P<0.01). HOMA-IR was only correlated with body fat mass in DEXA(r=0.341, P<0.05). Conclusion : This study revealed that G/I ratios had a statistically significant correlation with anthropometric obesity indices(OD and BMI) and also had a correlation with both body fat mass and percentage of fat. These results suggest that G/I ratios could be used as useful index when obese children and adolescence are followed up.

Dynamic Profile of the Copper Chaperone CopP from Helicobacter Pylori Depending on the Bound Metals

  • Hyun, Ja-shil;Park, Sung Jean
    • Journal of the Korean Magnetic Resonance Society
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    • v.20 no.3
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    • pp.76-81
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    • 2016
  • Copper is an elemental ion in living organisms. CopP from Helicobacter Pylori (HpCopP) is a copper(I)-binding protein and was suggested as regulator of copper metabolism in vivo. Previously, the metal binding property of HpCopP for Ag(I), Cu(I), and Cu(II) as well as the tertiary structure of HpCopP was shown. In this study, the dynamic profiles of HpCopP depending on metal binding were studied using ${^1H}-^{15}N$ steady-state NOE analysis. The heteroNOE experiment was performed for apo-CopP or metal-bound CopP. The obtained NOE values were analyzed and compared to figure out the effect of metals on the structural flexibility of HpCopP. As a result, Ag(I) and Cu(I) ions improved the rigidity of the structure while Cu(II) ion increased the flexibility of the structure, suggesting the oxidation of the CXXC motif decreases the structural stability of HpCopP.

Quicksort Using Range Pivot (범위 피벗 퀵정렬)

  • Lee, Sang-Un
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.4
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    • pp.139-145
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    • 2012
  • Generally, Quicksort selects the pivot from leftmost, rightmost, middle, or random location in the array. This paper suggests Quicksort using middle range pivot $P_0$ and continually divides into 2. This method searches the minimum value $L$ and maximum value $H$ in the length n of list $A$. Then compute the initial pivot key $P_0=(H+L)/2$ and swaps $a[i]{\geq}P_0$,$a[j]<P_0$ until $i$=$j$ or $i$>$j$. After the swap, the length of list $A_0$ separates in two lists $a[1]{\leq}A_1{\leq}a[j]$ and $a[i]{\leq}A_2{\leq}a[n]$ and the pivot values are selected by $P_1=P_0/2$, $P_2=P_0+P_1$. This process repeated until the length of partial list is two. At the length of list is two and $a$[1]>$a$[2], swaps as $a[1]{\leftrightarrow}a[2]$. This method is simpler pivot key process than Quicksort and improved the worst-case computational complexity $O(n^2)$ to $O(n{\log}n)$.

Complete Moment Convergence of Moving Average Processes Generated by Negatively Associated Sequences

  • Ko, Mi-Hwa
    • Communications for Statistical Applications and Methods
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    • v.17 no.4
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    • pp.507-513
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    • 2010
  • Let {$X_i,-{\infty}$ < 1 < $\infty$} be a doubly infinite sequence of identically distributed and negatively associated random variables with mean zero and finite variance and {$a_i,\;-{\infty}$ < i < ${\infty}$} be an absolutely summable sequence of real numbers. Define a moving average process as $Y_n={\sum}_{i=-\infty}^{\infty}a_{i+n}X_i$, n $\geq$ 1 and $S_n=Y_1+{\cdots}+Y_n$. In this paper we prove that E|$X_1$|$^rh$($|X_1|^p$) < $\infty$ implies ${\sum}_{n=1}^{\infty}n^{r/p-2-q/p}h(n)E{max_{1{\leq}k{\leq}n}|S_k|-{\epsilon}n^{1/p}}{_+^q}<{\infty}$ and ${\sum}_{n=1}^{\infty}n^{r/p-2}h(n)E{sup_{k{\leq}n}|k^{-1/p}S_k|-{\epsilon}}{_+^q}<{\infty}$ for all ${\epsilon}$ > 0 and all q > 0, where h(x) > 0 (x > 0) is a slowly varying function, 1 ${\leq}$ p < 2 and r > 1 + p/2.

Cloning and Expression in E. coli of the HOPDA Hydrolase Gene from Pseudomonas sp. P20

  • Lim, Jong Chul;Chae, Jong Chan;Kim Youngsoo;Kim, Hyong Bai;Kim Chi Kyung
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.349-354
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    • 1996
  • Pseudomonas sp. P20 is a natural isolate which is capable of degrading biphenyl and 4-chlorobiphenyl. From a clone of pCK1022 harboring pcbCD genes of Pseudomonas sp P20, a pcbD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA) hydrolase was subcloned in Escherichia coli XL-1-Blue by using pBluescript SK(+) vector. The 2.8-kb HindII fragment harboring the pcbD gene cloned in pCK 1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 carrying pCK0124 degraded HOPDA to benzoate and 2-hydroxypenta-2, 4-dienoate by HOPDA hydrolase encoded by pcbD gene as effectively as E coli CK 1022 HARBORING pcbCD genes.

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