• Preventive Nutrition and Food Science
• /
• v.10 no.1
• /
• pp.11-16
• /
• 2005

Enzymatic hydrolysis of dark muscle of skipjack was optimized by using response surface methodology. Three factors of independent values were pH (4.2 to 9.8), time (0.6 to 3.4 hrs) and temperature (34℃ to 76℃), and independent values were optical density and brix. The optimum conditions for enzymatic hydrolysis were pH 7.0 to 8.0, 55℃ and 3 hrs. The headspace volatile compounds of reaction flavors using the enzymatic hydrolysate, cysteine and xylose were identified by using the combination of a canister system, gas chromatography and mass selective detector. Among 67 compounds, we identified 8 sulfur-containing compounds and 7 furans which were thought to be highly related to meat-like flavors.

• Journal of Microbiology and Biotechnology
• /
• v.34 no.4
• /
• pp.969-977
• /
• 2024

Indigo is a valuable, natural blue dye that has been used for centuries in the textile industry. The large-scale commercial production of indigo relies on its extraction from plants and chemical synthesis. Studies are being conducted to develop methods for environment-friendly and sustainable production of indigo using genetically engineered microbes. Here, to enhance the yield of bioindigo from an E. coli whole-cell system containing tryptophanase (TnaA) and flavin-containing monooxygenase (FMO), we evaluated tryptophan transporters to improve the transport of aromatic compounds, such as indole and tryptophan, which are not easily soluble and passable through cell walls. Among the three transporters, Mtr, AroP, and TnaB, AroP enhanced indigo production the most. The combination of each transporter with AroP was also evaluated, and the combination of AroP and TnaB showed the best performance compared to the single transporters and two transporters. Bioindigo production was then optimized by examining the culture medium, temperature, isopropyl β-D-1-thiogalactopyranoside concentration, shaking speed (rpm), and pH. The novel strain containing aroP and tnaB plasmid with tnaA and FMO produced 8.77 mM (2.3 g/l) of bioindigo after 66 h of culture. The produced bioindigo was further recovered using a simple method and used as a watercolor dye, showing good mixing with other colors and color retention for a relatively long time. This study presents an effective strategy for enhancing indigo production using a combination of transporters.

• Journal of Multimedia Information System
• /
• v.8 no.1
• /
• pp.31-34
• /
• 2021

The classical HMM is defined by a parameter triple �� = (��, A, B), where each parameter represents a collection of probability distributions: initial state, state transition and output distributions in order. This paper proposes a new stationary parameter e = (e1, e2, …, eN) where N is the number of states and et = P(|xt = i, y) for describing how an input pattern y ends in state xt = i at time t followed by nothing. It is often said that all is well that ends well. We argue here that all should end well. The paper sets the framework for the theory and presents an efficient inference and training algorithms based on dynamic programming and expectation-maximization. The proposed model is applicable to analyzing any sequential data with two or more finite segmental patterns are concatenated, each forming a context to its neighbors. Experiments on online Hangul handwriting characters have proven the effect of the proposed augmentation in terms of highly intuitive segmentation as well as recognition performance and 13.2% error rate reduction.

• Journal of Gastric Cancer
• /
• v.19 no.3
• /
• pp.329-343
• /
• 2019

Purpose: Gastric cancer with lymph node metastasis (LNM) more than 15 (N3b) was defined as stage IV until the 6th AJCC system. However, it has been reclassified as a localized disease (stage IIb or III) since the 7th system. The aim of this study is to demonstrate that the survival of N3b is comparable to cytology-only positive (CY1-only) stage IV and to propose a new TNM system interpreting N3b as an eligibility criterion for receiving more intensive chemotherapy regimens. Materials and Methods: 1,430 patients who underwent gastric cancer surgery at Seoul National University Hospital from 2007 to 2012 were retrospectively analyzed. The 5-year survival rate (5YSR) and 3-year recurrence-free survival (RFS) were evaluated according to the 7th and 8th systems, as well as a new categorization based on N-classification; N0-2 (LNM<7), N3a (LNM 7-15), or N3b (LNM>15). Results: The survival of N3b is comparable to that of CY1-only stage IV (log rank test, P=0.671) and is distinct from that of grossly stage IV (log rank test, P<0.001). The survival of the remaining stage IIIc (T4bN3a) was comparable to those of N3b and CY1-only stage IV. Most N3b patients had significantly shorter 3-year RFS and mean RFS than those with IIb-IIIc, as if N3b itself was a higher TNM stage. Conclusions: In terms of survival, T4bN3a, N3b, and CY1-only stage IV were unified as stage IVa, while grossly stage IV was defined as stage IVb. N3b can be regarded as an eligibility criterion for undergoing more intensive chemotherapy regimens.

• Applied Chemistry for Engineering
• /
• v.21 no.5
• /
• pp.559-563
• /
• 2010

The representation of color with natural dyes is closely related with the modern well-being life. This study is focused on the representation of target color of the blue, yellow, and red color system, these color sources were originated from the green tea, gardenia, and black rice respectively. The quantitative numerical value of brightness (L), color coordinates (a, b) were designated for the target color, the optimum color nearest to the target value was extracted with the solvent pH, temperature, and extraction time. The longer the extraction time had more thickening color in case of the gardenia. The L-a-b coordinate of extracted color were analyzed with the color difference meter and compared with the target color. The experimental variables were optimized to extract the color of the smallest ${\Delta}E$ with the target color. As a result, the CIE standard value was proposed and the color was represented.

• Microbiology and Biotechnology Letters
• /
• v.19 no.4
• /
• pp.356-361
• /
• 1991

- To investigate the possibility of genetic development for a multi-purpose strain of Bacillus subtilis YBL-7 against Fusat-iurn solani causing root rot of many impotant corps, the plasmid pGU66 inserting urease gene of Bacillus pasteurii had been introduced into Bacillus subtilis YBL-7 by PEG-induced protoplast (PIP) transformation system. Protoplasts of B. subtilis YBL-7 were prepared by treating the cells with lysozyme (200 $\mu g$/ml) in hypertonic buffer (SMMP). The highest transformation frequency was achieved when cells of the strain with lysozyme at $42^{\circ}C$ for 90 minutes. Optimal transformation was obtained using polyethylene glycol (MW 4000) at final concentration of 30% (V/V). The transformation frequency was increased proportionally to 1.2 $\mu g$ of plasmid DNA. At best condition, the transformation frequency (transformants/ regenerants/$\mu g$ of DNA) for pGU66 was appoximately $4 \times 10^{-3}$. Also, the urease gene was strongly expressed in the transformants of B. subtilis YBL-7 and maintained steadily. The antifungal ability of transformant was very similar to that of B. ssubtilis YBL-7.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.27 no.1
• /
• pp.17-27
• /
• 2001

A cell-based assay system for monitoring NF-$textsc{k}$B activity was developed to determine the influence of activated NF-$textsc{k}$B in human HaCaT cells. The pNF-$textsc{k}$B-SEAP-NPT plasmid that permits expression of the secreted alkaline phosphatase (SEAP) reported gene in response to the NF-$textsc{k}$B activity and contains neomycin phosphotransferase (NPT) gene for the geneticin resistance in host cells was constructed and transfected into human keratinocyte cell line HaCaT. Human HaCaT transfectant cells secreted the SEAP enzyme into the culture medium in a time-dependent manner until 72h. NF-$textsc{k}$B activities were measured in the SEAP reporter gene assay using a fluorescent detection method. The treatment of HaCaT cell transfectants with known antioxidants [e.g., N-acetyl-L-cysteine and vitamin C] showed inhibition of NF-$textsc{k}$B activity in a time-and concentration-dependent manner. The phorbol 12-myristate 13-acetate (PMA) known as a stimulator of NF-$textsc{k}$B expression demonstrated that it increased NF-$textsc{k}$B activity in a time- and concentration-dependent manner. This assay system could be used to determine the quantitative measurement of NF-$textsc{k}$B activity in the human skin and allow the screening of anti-inflammatory agents from various synthetic chemicals and natural products for dermatological purpose. Abbrevitions used: NF-$textsc{k}$B, nuclear factor kappa B; I-$textsc{k}$B, Inhibitory kappa B; SEAP, secreted alkaline phosphatase; NPT, neomycin phosphotransferease; PCR, polymerase chain reaction: dNTP, deoxynucleoside triphosphates; DMEM, dulbecco’s modified eagle medium; FBS, fetal bovine serum; PBs, phosphate-buffered saline; MUP, 4-methylumbellifery phosphate; NAC, N-acetyl-L-cysteine; DMSO, dimethyl sulfoxide; PMA, phorbol 12-myristate 13-acetate.

• Journal of The Korean Astronomical Society
• /
• v.43 no.5
• /
• pp.153-159
• /
• 2010

Color excesses of classical Cepheids in the uvby color system are estimated for the calibration stars with distances from the literature that are measured independently. Intrinsic photometric indices for these stars are calculated and a calibrated empirical relation between (b - y)$_0$, period, [$c_1$], and [$m_1$] is derived through a linear fit. This relation is used to determine color excesses E(b-y) for 59 Cepheids. We also examine the period-color [log P : (b - y)$_0$] relation, and find no signs of nonlinearity. We estimate the effective temperature and surface gravity of several Cepheids using both Kurucz and MARCS/SSG grids for [Fe=H]=0.0. We confirm that both temperature and surface gravity are higher, by about 150K and 0.4 respectively, when the MARCS/SSG atmospheric grids are used.

• The Transactions of the Korean Institute of Electrical Engineers B
• /
• v.49 no.10
• /
• pp.665-674
• /
• 2000

This paper deals the HVDC system stability according to controller types. In this paper, in order to evaluate the stability, some important properties of the HVDC. such as $d{\tau}/d{\alpha}\;,\;dV/d{\alpha}$ and dP/dI stability in different control modes, are analysed. Furthermore, Maximum available power according to controller types are calculated. This paper shows a generated formulation of control sensitivity for control modes, aids in the design of the controller and the operating mode determination. Finally, a simulation is performed by Math/CAD, and a simulation results are verified.

• Proceedings of the KIEE Conference
• /
• 1998.07f
• /
• pp.2048-2050
• /
• 1998

An uninterruptible power supply(UPS) with parallel operation is used to increase the power capacity of the system or to secure higher reliability at critical loads. In the parallel operating system composed of the multiple UPSs, load-sharing, i.e. current balance control between them is key technique. Because of its low impedance and quick response characteristics, inverter output current changes very rapidly and thereby easily researches an overload condition. The difference between total load current divided by number of operating inverters and its own current is detected as unbalanced current. Then frequency and voltage are controlled to minimize the active component and the reactive component. A good performance of the proposed load-sharing technique is verified by experiments in the parallel operating system with two 40kVA UPSs.