• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.116-121
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• 2012

Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is $82{\times}75mm$, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under $22^{\circ}C$, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under $10^{\circ}C$. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2003.10a
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• pp.100-100
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• 2003

• Journal of Sericultural and Entomological Science
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• v.43 no.2
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• pp.83-87
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• 2001

This study was carried out to investigate the optimal medium and optimal dextrose concentration of 12 entomopathogenic fungi. The colonies of Cordyceps gracilioides, C. sp.(J23), Paecilomyces farinosus(J5), P tenuipes, Paecilomyces sp.(J4), Beauveria bassiana and B. brongniartii on SLA(silkworm larva agar) medium are similar to those on PDA(Potato dextrose agar) medium. On the contrary, the colonies of 5 fungi are very various according to its medium. The optimal medium for 1 1 fungi was SLA-A medium, which was better than PDA medium with regard to the mycelial growth. The optimal concentration on mycelial growth was superior to 6% for 6 fungi. When silkworm larvae was injected with P. tenuipes on SLA medium for fruiting body production. Infection rate, fruiting body formation rate, synnemata number and synnemata length were 91.3 $\pm$ 1.5%, 88.6 $\pm$ 2.3%, 56.6 $\pm$ 3.2 and 33.4 $\pm$ 2.88 ㎜ respectively. In economical analysis, the prototype medium can reduce 61.9% of production cost per 1 liter compared with PDA medium.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.1-16
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• 2021

The phylogenetic relationships among thirty-two strains (P1~P32; including Cordyceps sp., Paecilomyces sp., Beauveria sp., Aranthomyces sp., Isaria sp. and Himenostilbe sp.) in Miryang region located in the southern part of Korea, were investigated based on internal transcribed spacer (ITS) sequences of ribosomal DNA. A fragment of ITS region was amplified by polymerase chain reaction (PCR) using the specific primer pairs ITS1 and ITS4. After obtained same size of PCR products from various strains, we cloned them into a pGEM-T easy vector to determine their sequences. BLAST analyses of the nucleotide sequence ITS1, 5.8S and ITS2 gene fragments revealed the identity and their phylogenetic relationship. Among 32 strains isolated from Miryang region, Cordyceps militaris was shared 100% sequences with Genbank (AY49191, EU825999, AY491992), while some species are not shared perfectly with reported sequences. For example, strain P17 (P. tenuipes in Ulju-gun Gaji Mountain) has some differences among the other strains of P. tenuipes (Miryang-si Jocheon-eup, Miryang-si Gaji Mountain) and those of gene bank. We conclude that ITS analyses with strains in the suburbs of Miryang in this study can be effectively used as a tool for classification, evaluation and collection of the natural eco-type genetic resources.

• Journal of Mushroom
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• v.22 no.1
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• pp.1-7
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• 2024

Mushrooms play crucial roles as reservoirs of naturally occurring bioactive compounds. Among these, Cordyceps militaris is significant because of its well-established reputation for organoleptic excellence and positive health effects, which have led to its widespread commercialization. In contrast, the key properties of Paecilomyces variotii, an ectomycorrhizal symbiont, has received limited attention. In alignment with current research trends, the study of the mycelia and culture media of these mushrooms hold promise in identifying potential sources of valuable bioactive compounds. In the present study, we investigated C. militaris and P. variotii for their phenolic acids and sterols, assessing antioxidant capacity, anti-inflammatory effects, and anti-proliferative activity. Interestingly, P. variotii mycelia exhibited higher concentrations of ergosterol and phenolic compounds, with comparable levels observed in the fruiting bodies, along with superior antioxidant activity compared to that of C. militaris. In contrast, C. militaris mycelia demonstrated anti-inflammatory effects (which were absent in P. variotii mycelia) and cytotoxicity comparable to, and at times exceeding, that of its fruiting bodies (in contrast to P. variotii). In addition, the species analyzed in this study displayed variations in growth rates and mycelial production, which merit consideration for potential future applications and further study.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.71-74
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• 2003

Superoxide dismutase (SOD), one of the essential element of the antioxidant defense system, mainly removes $O^{-10}$ $_2$ and also prevents $O^{-10}$ $_2$ mediated reduction of iron and subsequent OH$^{-10}$ generation, which is highly toxic to the organism. Of these SOD enzymes, Cu, Zn-containing SOD (SODI) is an important component of the antioxidant defense system in eucaryotic cells. The SODI enzyme binds one copper and one zinc ion and displays the Greek Key $\beta$-barrel fuld. (omitted)

• The Korean Journal of Ecology
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• v.24 no.5
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• pp.315-322
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• 2001

Seasonal production and nutrient concentrations of mushrooms in an Quercus acutissima forest were studied from 1999 to 2000. Thirty 2×2m quadrats were established randomly in the study area. 114 species of mushrooms were identified during the study period. Although mushrooms occurred from May to November in each year, the highest biomass production occurred in August. Seasonal dominant species was Lactarius violascens in June, R. bella and L. violascens in July, Marasmius siccus in August, Cortinarius tenuipes in September, Lepista nuda and C. tenuipes in October, respectively. Annual production of mushrooms in 1999 and 2000 were 84.8 kgDW·ha/sup -1/·yr/sup -1/ and 86.7 kgDW·ha/sup -1/·yr/sup -1/, respectively. Seasonal production was27.1kg/ha for July, 35.9kg/ha for August and 17.1 kg/ha for September, 3.7kg/ha for October, respectively. Seasonal dominant species in biomass was Russula alboareolata in June, R. bella in July, R. pseudodelica in August and September, Lepista nuda in October, respectively. R. pseudodelica showed the highest biomass in the study area, which is 17.5% of the total biomass. Average concentration of nutrients in mushrooms was 45.2mg/g for N, 1.5 mg/g for P, 29.6 mg/g for K, 0.2 mg/g for Ca and 0.8 mg/g for Mg, respectively, which were much higher than those in the leaf litter. Nutrients absorbed by mushrooms in 2000 was 4,163.1 g·ha/sup -1/·yr/sup -1/ for N, 130.1 g·ha/sup -1/·yr/sup -1/ for P, 2,568.2 g·ha/sup -1/·yr/sup -1/ for K, 12.4 g·ha/sup -1/·yr/sup -1/ for Ca, 68.5 g·ha/sup -1/·yr/sup -1/ for Mg, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.125-132
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• 1999

This study has investigated the growing features and vegetation of Acanthopanax chiisanensis and Acanthopanax koreanum in Mt. Jiri and Halla from Sep. 1997 to Aug. 1998. As to Acanthopanax chiisanensis, it occupied surrounding areas of Nogodan in Mt. Jiri of the height of 1,507m, ImgeolyoungJae of 1,300m and the Mt. Halla. of 1,100m of There were rather high constancy degree with Quercus mongolica, Schizandra chinensis, Hydrangen serrate for. acuminata, Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Actaea asiatica, Thalictrum filamentosum, Abies koreana, Arundinella hirta from the sorts. The common species in Mt. Jiri and Halla are Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Callicarpa japonica, Parthenocissus tricuspidata, Acer mono, Ligularia fischeri, Disporum smilacinum, Viola acuminata Aconitum kusnezofii. On the other hand, concerning of Acanthopanax koreanum community, it possessed the small areas of wedo weoltae village in Chejudo. In this area, a liana e.g. Dioscorea tenuipes, Hedera rhombea, Lygodium japonicum, Akebia quinata, Vitis thunbergii, Dunbaria villosa, Trachelospermum asiaticum var. intermedium, Vitis amurensis, Paederia scandens, lives with Pinux thunbergii, Celtis aurantiaca, Mallotus japonicus and so forth in tree layer and subtree layer. There is artificial change no move in the researching sites, Acanthopanax chiixanensis will be transformed into Abies koreana - Acanthopanax chiisanensis community. Soil characteristics showed the high value such as pH 6.56 and conductivity 0.258mmho/cm in Acanthopanax koreanum community of Mt. Jiri, they also indicated digit as follows: the content of organic matter and total nitrogen are from 25.16 through 25.35% and 7.58 through 9.30mg/g respectably. The soil characteristics at Acanthopanax chiisanensis community in Mt. Jiri and Halla, was high as like pH 5.76, conductivity 0.238mmho/cm, total nitrogen 9.30mg/g, total phosphorus 0.126mg/g in Mt. Halla.