• Title/Summary/Keyword: P-max

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대두 (Glycine max) 부배유 유래 칼루스의 원형질체 분리 및 배양 (Isolation and Culture of Protoplasts from Hypocotyl-derived Callus of Soybean (Glycine max))

  • 이광웅
    • Journal of Plant Biology
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    • 제28권3호
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    • pp.233-241
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    • 1985
  • The isolation and culture of protoplasts from hypocotyl-derived calluses of Glycine max (L.) Merr. cv. Jangyeop were obtained by digestion for 6 hrs in an enzyme solution containing 3.5% cellulase, 1.5% macerozyme, 10% sorbitol and 0.1% CaCl2.2H2O at pH 5.8. Newly formed cell wall of protoplasts cultured in MS agar medium containing 10 $\mu$M $\alpha$-naphthaleneacetic acid (NAA) and 32 $\mu$M N6-benzylaminopurine (BAP) could be observed after 24 hrs culture. The first cell division of the protoplasts was observed after 3 days of culture; cell clusters after 2 weeks of culture. When transferred to solid media, the protoplasts formed cell clusters gave rise to proliferating calluses.

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암환자의 희망 예측요인 (Predictive Factors of Hope in Patients with Cancer)

  • 이화진;손수경
    • 성인간호학회지
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    • 제12권2호
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    • pp.184-195
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    • 2000
  • It has been believed that cancer is an omnious factor threatening the future and life itself. Patients having the disease experience anxiety, fear, feeling of weakness, depression and feelings of uncertainty and hopelessness. Most cancer patients, however, have expectations of possible recovery and a better future, very different from the patients who feel hopeless. Therefore. hope allows people to respond effectively to the fatal disease they have and prevents them from detoriorating physically and spiritually, positively influencing their survival, response to treatment and sense of security. Studies previously performed showed that hope is positively correlated with social and family supports, self-esteem, spiritual well-being, responsive action, health promotion behavior and quality of life. Thus, the study attempted to provide basic information on nursing cancer patients by investigating their levels of hope and determining predictive factors which influence hope. For the study 200 cancer patients in two university hospitals located in Pusan were sampled as subjects. Data were collected for twenty nine days from Feburary 1, 1999 to March 1. Instrumets for the study included 10 items from the self-esteem scale by Rosenberg (1965), 39 hope measurements by Kim and Lee(1965), 16 of the social support scale by Tae(1986) and 16 of the general characteristics scale, all of which totaled 81 items. The data were analyzed using the SPSS program. General characteristics of the investigated based on numbers and percentage. Hope, self-esteem and social support were analyzed using means, minimum, maximum and standard deviation. Relations among the foregoing three factors were analyzed using Pearson' correlation coefficient. Levels of hope in cancer patients were determined using t-test, ANOVA and Scheffe test. Predictive factors influencing hope were investigated using multiple stepwise regression analysis. Results of the study are summarized as follows: 1. An average level of hope was $185.55{\pm}23.39$ points(96 min. and 234 max.) 2. Levels of hope showed a significant difference among them according to sex (t=-3.69, P=.000), age(F=4.714, P=.000), job(F=3.247, P=.008), monthly income (F=6.113, P=.003), treatment charge (F=3.796, P=.011), supportive resources (F=10.554, P=.000), diagnosis(F=2.287, P=.029), perceived health status(F=22.184, P=.000), level of pain(F=3.334, P=.021), religion (F=4.911, P=.001) and religion's effect in life (F=11.706, P=.000), 3. For the subjects, self-esteem and social support were $38.32{\pm}7.21$(13 min, and 50 max.) and $52.97{\pm}8.49$points(28 min, 80 max.). Concerning social support, average levels of family support and medical support were found $35.95{\pm}6.05$(18 min, and 40 max) and $27.02{\pm}4.99$ points(20 min and 40 max). The hope the cancer patients showed significant correlations with self-esteem (r=.588, P=.000), family support(r=.224, p=.001) and medical support(r=.221, P=.002). 4. The five variables related to hope (self-esteem, religion's effect in life, perceived health status, social support and age) accounted for 54.2 percent of the hope level; especially, self-esteem was the highest at 34.6%. As shown in the above results, predictive factors which most influence hope in cancer patients were self-esteem and religion's effect of life. Therefore, nursing interventions to increase self-esteem should be developed. Regarding religion's effects, studies on spiritual aspects should be carried out in a way that contributes to promotion of hope.

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양식을 위한 먹이사료로서의 Yeast 균주의 개발: 가능성 있는 효모 균주 (Development of Yeast Strains as Feed for Aquaculture: Possible Yeast Strains)

  • 문정혜;탁건태;김중균
    • 생명과학회지
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    • 제6권2호
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    • pp.135-141
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    • 1996
  • 양식을 위한 먹이사료로서 가능성 있는 효모균주를 연구하였다. Kluyveromyces fragilis효모와 Candida utilis 효모의 최대 비증식 속도와 바이오매스 효율은 Saccharomyces cervisiae 효모에 비하여 굉장히 높은 값을 나타내었다. 따라서, 이 연구는 대량생산을 위하여, 플라스크 배양을 통한 이들 두 효모균들의 성장특성에 그 초점을 맞추었다. 5% 접종량을 가지고 실험한 결과, 가장 좋은 $\mu$$_{max}$ 와 OD$_{max}$ 값들은 K, fragilis는 2.5% fructose 배지에서, C. utilis는 2% YE배지에서 각각 얻었으며, 이 때의 이 값들은 K. fragilis는 0.73 hr$^{-1}$1 와 3.00을 C. uitilis는 0.59 hr$^{-1}$ 와 2.80으로 밝혀졌다. 또한, 아연과 염의 초기종도가 증가할수록 균성장에 있어서의 우도기는 증가하였으며, 접종량이 증가할수록 유도가는 감소하였다. 두 효모균은 3.5% 염농도에서 비교적 잘 자랄 수 있었으며, C. utilis 효모균만이 아연을 이용할 수 있었다.

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Glycine max Fermented by a Novel Probiotic, Bifidobacterium animalis subsp. lactis LDTM 8102, Increases Immuno-Modulatory Function

  • Kim, Jae Hwan;Jeong, Minju;Doo, Eun-Hee;Koo, Young Tae;Lee, Seon Joo;Jang, Ji Won;Park, Jung Han Yoon;Huh, Chul Sung;Byun, Sanguine;Lee, Ki Won
    • Journal of Microbiology and Biotechnology
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    • 제32권9호
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    • pp.1146-1153
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    • 2022
  • Many probiotic species have been used as a fermentation starter for manufacturing functional food materials. We have isolated Bifidobacterium animalis subsp. lactis LDTM 8102 from the feces of infants as a novel strain for fermentation. While Glycine max has been known to display various bioactivities including anti-oxidant, anti-skin aging, and anti-cancer effects, the immune-modulatory effect of Glycine max has not been reported. In the current study, we have discovered that the extract of Glycine max fermented with B. animalis subsp. lactis LDTM 8102 (GFB 8102), could exert immuno-modulatory properties. GFB 8102 treatment increased the production of immune-stimulatory cytokines in RAW264.7 macrophages without any noticeable cytotoxicity. Analysis of the molecular mechanism revealed that GFB 8102 could upregulate MAPK2K and MAPK signaling pathways including ERK, p38, and JNK. GFB 8102 also increased the proliferation rate of splenocytes isolated from mice. In an animal study, administration of GFB 8102 partially recovered cyclophosphamide-mediated reduction in thymus and spleen weight. Moreover, splenocytes from the GFB 8102-treated group exhibited increased TNF-α, IL-6, and IL-1β production. Based on these findings, GFB 8102 could be a promising functional food material for enhancing immune function.

육상과 선박내에서의 Circuit Weight Training 이 근기능 및 심폐기능에 미치는 영향 (The Effect of Circuit Weight Training on the Muscular Function and Cardiopulmonary Function)

  • 하해동;신군수
    • 한국항해항만학회:학술대회논문집
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    • 한국항해항만학회 1998년도 춘계학술발표회 논문집
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    • pp.163-185
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    • 1998
  • The purpose of this study was to analyze the physiology changes by circuit weight training(CWT). The subject of this study consists of 16 student on ship(experimental groups(SE) N=8, control group(SCa) N=8) and 16 students on land (experimental groups(LE) n=8, control groups(LCb) N=8). The items of measurement were the changes of CWT time and heart rate per week, musclear functino and cardiopulmonary function. The conclusions are as follows : 1. Muscular fuction 1) Back strength in SE and LE group was increased significantly (p<.01, p<.05, 8.86% and 7.08%).Grip strength was increased slightly in 4 groups but there was no significance. 2) In push-ups, all 4 groups were increased significantly (p<.05, p<.01), sit-ups also were increased significantly in SE and LE group (p<.05, 6.71% and 9.62%). 3) In SE and LE group , standing long jump was in significantly (p<.01, p<.05, 4.49% and 6.09%), but only in LE group , side step was increased significantly (p<0.1, 5.84%). 2. Cardiopulmonary function 1) HRrest was decreased slightly in all 4 groups but was not significant changes. HRmax was increased only in LE group significantly (p<.05, 2.81%), treadmill running time was increased significantly in SE and LE group respectively (p<.01, p<.05, 10.78% and 11.07%). 2) VEmax was increased significantly in SE and LE group(p<.05, p<.01, 10.59% and 13.68%), but only in LE group Rfmax was increased significantly (p<.01, 4.83%). 3) In VO2max, LCb group was increased significantly (p<.05, 6.835), but SCa group was decreased significantly (p<.05, 4.32). VO2max/kg$.$min was increased significantly in LE and LCb group respectively (p<.01 p<.05. 4.75% and 3.98%).

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Multistatic Field또는 3차원 공용보상체를 사용한 유방의 방사선 조사법의 평가 (Improved Breast Irradiation Techniques Using Multistatic Fields or Three Dimensional Universal Compensators)

  • 한영이;조재호;박희철;추성실;서창옥
    • Radiation Oncology Journal
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    • 제20권1호
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    • pp.24-33
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    • 2002
  • 목적 : 유방보존 수술 후 행하는 방사선치료인 2문 대칭조사(two tangential field technique)시 나타나는 선량분포의 불균일성을 효과적으로 개선하기 위하여, multistatic fields의 사용법과 공용보상체 사용법을 제시하고 평가하였다. 대상 및 방법 : 1) Multistatic field 방법 3차원의 치료계획용 software (RTP)를 이용하여, wedge를 사용한 2문 대칭 조사의 최적의 치료계획을 시행한 후 beam's eye view상에서 과조사가 일어나는 부분을 가리워 주도록 blocked field를 설계하고, 그 beam에 대칭되는 beam을 만들었다. 기존의 2개의 tangential field와 추가된 field의 weighting을 최적의 선량분포를 갖도록 조절하였다. 2) 공용보상체 사용법 : 1999와 2000년에 본원에서 whole-breast radiotherapy를 받은 환자 20명의 유방의 크기를 측정하고 평균하여 표준 유방 모델을 만들었다. 이 모델에 대하여 공용보상체를 설계하고, 설계된 공용보상체의 geometry를 RTP에 입력한 후 환자의 치료 계획을 수행하였다. 2문 대칭조사 치료 계획과, multistatic fields의 경우 그리고 공용 보상체를 사용한 경우의 치료계획에서의 불균일도(DII : 처방선량의 $95-105\%$를 벗어나는 PTV의 부피의 백분율), 최대선량 값$(D_{max})$ 그리고 등가선량 곡선을 각각 비교하였다. 결과 : Multistatic field 방법은 DII의 평균값을 $14.6\%$ (p value<0.000) 낮추고 $D_{max}$$4.7\%$ (p value<0.000) 낮춤으로써, 전통적인 2문 대칭 조사법보다 우수한 방법으로 확인되었다. 반면에 공용보상체의 사용은 평균 DII를 $3.7\%$ 낮추지만(p value=0.260) 평균 $D_{max}$는 거의동일 하여($0.3\%$ 감소, p value=0.867), 전통적인 방법보다 우수성이 크게는 없는 것으로 평가되었다. 그러나 환자의 체곡선이 보상체와 잘 일치하는 경우에는 DII가 $18\%$까지 감소하였다. 결론 : Multistatic field 방법은 모든 환자에 대하여 선량분포의 균일성을 전반적으로 향상시키는 효과적인 방법으로 평가되는 반면 공용보상체의 사용은 보상체의 크기가 환자의 체 윤곽과 잘 일치하는 경우만 효과적으로, 적용의 범위에는 한계가 있는 방법으로 평가되었다.

MaxEnt 모형을 활용한 부산광역시 내 오동나무 및 참오동나무의 분포 경향과 생태적 특성 (Distribution Patterns and Ecological Characters of Paulownia coreana and P. tomentosa in Busan Metropolitan City Using MaxEnt Model)

  • 이창우;이철호;최병기
    • 한국전통조경학회지
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    • 제35권2호
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    • pp.87-97
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    • 2017
  • 오동나무는 한국 전통 문화에서 오래전부터 인식되어 왔으며, 다양한 분야에서 종의 가치에 대해 연구되어 왔다. 그러나 종의 분포와 생태적 특성에 대한 연구는 미흡한 상황이다. 본 연구는 MaxEnt 모형을 활용하여 부산광역시 내 오동나무 두 종의 분포 경향 및 생태적 특성을 밝히는데 목적을 두고 있다. MaxEnt 모형은 현장 조사로 수집된 오동나무 종의 위치 정보와 지형, 기후, 잠재인간간섭도와 같은 환경 인자로 구축되었다. 연구결과 AUC 값은 오동나무와 참오동나무가 각각 0.809으로 모형의 정확도가 적절한 것으로 확인되었다. 분포모형에 따른 연구지역 내 오동나무와 참오동나무의 분포 경향은 두 종 모두 시가지, 나지가 밀집해 있는 도심위주의 분포를 나타냈다. 두 종의 잠재분포가능면적은 오동나무 $137.4km^2$, 참오동나무 $135.0km^2$로 확인되었으며, 중구, 동래구, 부산진구, 연제구 등에서 높은 확률로 분포하였다. 환경요인의 기여도 분석 결과, 오동나무와 참오동나무의 분포에 잠재인간간섭도가 약 50% 내외의 기여를 하는 것으로 확인되었고, 잠재인간간섭도와 양의 상관관계를 나타냈다. 해발고도는 두 종 모두에서 음의 상관관계를 보였으며, 해발고도가 증가할수록 자연서식처에서 자생종과의 경쟁이 증가하기 때문인 것으로 판단된다. 본 연구의 결과들은 오동나무와 참오동나무의 분포가 인위적 활동에 의존되어 있음을 수리적으로 나타내는 결과이며, 한국 전통경관과의 관련성을 암시하는 결과이다. 이러한 결과는 추후 오동나무의 활용 및 보존, 복원에 있어서 의미 있는 정보를 제공할 수 있을 것으로 판단된다.

The Spectral Sharpness Angle of Gamma-ray Bursts

  • Yu, Hoi-Fung;van Eerten, Hendrik J.;Greiner, Jochen;Sari, Re'em;Bhat, P. Narayana;Kienlin, Andreas von;Paciesas, William S.;Preece, Robert D.
    • Journal of Astronomy and Space Sciences
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    • 제33권2호
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    • pp.109-117
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    • 2016
  • We explain the results of Yu et al. (2015b) of the novel sharpness angle measurement to a large number of spectra obtained from the Fermi gamma-ray burst monitor. The sharpness angle is compared to the values obtained from various representative emission models: blackbody, single-electron synchrotron, synchrotron emission from a Maxwellian or power-law electron distribution. It is found that more than 91% of the high temporally and spectrally resolved spectra are inconsistent with any kind of optically thin synchrotron emission model alone. It is also found that the limiting case, a single temperature Maxwellian synchrotron function, can only contribute up to 58+23−18% of the peak flux. These results show that even the sharpest but non-realistic case, the single-electron synchrotron function, cannot explain a large fraction of the observed spectra. Since any combination of physically possible synchrotron spectra added together will always further broaden the spectrum, emission mechanisms other than optically thin synchrotron radiation are likely required in a full explanation of the spectral peaks or breaks of the GRB prompt emission phase.

생쥐의 면역세포에 대한 검은콩 3종의 비교 연구 (Comparative Study of 3 kinds of Black Soybean on Murine Immune Cells)

  • 서승용;방금엽;은재순
    • 동의생리병리학회지
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    • 제24권4호
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    • pp.668-673
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    • 2010
  • The purpose of this research was the comparative study of 3 kinds of black soybean on murine immune cells. The 3 kinds of black soybean are Glycine max Merr. with inner color-yellow (GY), Glycine max Merr. with inner color-greenish (GG) and Rhynchosia volubilis Lour. (RV). All of the black soybean increased the viability of murine thymocytes in vitro. The combined treatment of GY or GG and mitogen did not affect the viability of splenic T- and B-lymphocytes compared with mitogen-treated group, but the combined treatment of RV and mitogen increased their action compared with mitogen-treated group. Also, the 3 kinds of black soybean were given p.o. once a day for 7 days, respectively. RV increased the population of thymic-$CD8^+$, splenic-$CD8^+$ and $B220^+$ cells in vivo. Furthermore, GY and GG did not affect the phagocytic activity and the production of nitric oxide in peritoneal macrophages in vitro, but RV enhanced their action. These results suggest that immunopotentiative action of Rhynchosia volubilis Lour. is more potent than their of Glycine max Merr.

Bioavailability of Digoxin Tablets in Healthy Volunteers

  • Lee, Chi-Ho;Park, Yun-Ju;Charies-D. Sands;Daniel-W. Jones;John-M. Trang
    • Archives of Pharmacal Research
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    • 제17권2호
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    • pp.80-86
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    • 1994
  • The bioavailability of digoxin generic tablets manufactures in Korea (formulations A & B) wwere compared to a standard (formulation C; Lanoxin brand digoxin, Burroughs Wellcome, USA) in 12 healthy Korean male volunteers (mean age 31.4 years) in a single dose, randomized, complete block crossover study. Using a latin square design, each of the subjects was randomized to the order number and allocated to each of the three treatments of 0.5mg oral digoxin. Digoxin conc4ntrations in serum and urine samples collected for 48 hours after dosing were measured by fluoprescence polarization immunoassy and radioimmunoassy, respectively. Treatments were compared by using nonlinear least squares regession analysis to evaluate the following pharmacokinetic parameters : maximum serum concentation $(C_{max})$; time of maximum serum concentation $(T_{max})$; area under the serum concentration-time curve $AUC_{0-12}$, $C_{max}$\;and\;(AUC_{0-12})$; and cummulative urinary excretion for 0-48 hours $(CLE_{0-48}.\;Mean\;AUC_{0-12},\;C_{max},\;and\;CUE_{0-48}$ values for formulations B and C were significantly different from formulation A (P<0.001), but not significantly diffeerent form each other. Basede on $AUL_{0-12}\;and\;CUE_{0-48}$ respectively, the relative availability of formulation B was 87.5% and 89.6% and the relative availability of formultation A was 43% and 35% when compared to formulation C(the standard).

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