• Structural Engineering and Mechanics
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• 제36권1호
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• pp.57-78
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• 2010

A novel flexibility-based 1D element that captures the material nonlinearity and second order P-$\Delta$ effects within a reinforced concrete frame member is developed. The formulation is developed for 2D planar frames in the modified fiber element framework but can readily be extended to 3D cases. The nonlinear behavior of concrete including cracking and crushing is taken into account through a modified hypo-elastic model. A parabolic and a constant shear stress distribution are used at section level to couple the normal and tangential tractions at material level. The lack of objectivity due to softening of concrete is addressed and objectivity of the response at the material level is attained by using a technique derived from the crack band approach. Finally the efficiency and accuracy of the formulation is compared with experimental results and is demonstrated by some numerical examples.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2004년도 가을 학술발표회 논문집
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• pp.281-287
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• 2004

In this paper, the refined plastic-hinge analysis accounting for gradual yielding with fibers on a section is developed. Geometric nonlinearities of member(P-δ) and frame(P-Δ) are accounted for by using stability functions. Residual stresses are considered by assigning initial stresses to the fiber on the section. The elastic core in a section is investigated at every loading step to determine the axial and bending stiffness reduction. The strain reversal effect is captured by investigating the stress change of each fiber. The proposed analysis proves to be useful in applying for practical analysis and design of three-dimensional steel frames.

• 한국통신학회논문지
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• 제23권8호
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• pp.2025-2031
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• 1998

MPEG2 동영상 압축 부호화는 비트율을 제어하는 방법에 따라서 압축율에 큰 영향을 미칠 뿐만 아니라 영상의 질도 좌우하게 된다. 본 논문은, I,P 프레임에 보다 많은 비트량을 할당하고, 인접 매크로블럭들의 MQUANT값들의 평균을 다음 매크로블럭의 양자화 값에 사용하는 비트 제어방법을 제안하였다. MPEG2 Test-Model5의 비트 제어방법과 비교하였을 때 제안한 방법은 약 0.9dB 정도의 화질이 향상됨을 보였다.

• 국제초고층학회논문집
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• 제2권2호
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• pp.141-152
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• 2013

This paper presents a brief overview of the recently developed performance-control method of moment frame design subjected to monotonously increasing lateral loading. The final product of any elastic-plastic analysis is a nonlinear loaddisplacement diagram associated with a progressive failure mechanism, which may or may not be as desirable as expected. Analytically derived failure mechanisms may include such undesirable features as soft story failure, partial failure modes, overcollapse, etc. The problem is compounded if any kind of performance control, e.g., drift optimization, material savings or integrity assessment is also involved. However, there is no reason why the process can not be reversed by first selecting a desirable collapse mechanism, then working backwards to select members that would lead to the desired outcome. This article provides an overview of the newly developed Performance control methodology of design for lateral resisting frameworks with a view towards integrity control and prevention of premature failure due to propagation of plasticity and progressive P-delta effects.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.135.2-136
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• 2003

To characterize plasmids in Xanthomonu axonopodis pv. glycines, we isolated plasmids pAG1 from the strain AG1 and pXAG81 and PXAG82 from the strain Bra, respectively, and sequenced three plasmids. The size of plasmids, pAG1, pXAG81, and pXAG82 was 15,149-base pairs (bp), 26,727-bp, and 1,496-bp, respectively Fifteen and twenty six possible open reading frames (ORFs) were present in pAG1 and pXAG81, respectively. Only one ORF homologous to a rep gene of Xylella fastidiosa was present in pXAG82. pAG1 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, three parA genes, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, two parA genes, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven hypothetical proteins. Based on DNA sequence analysis, we presume that pXAG81 is a conjugal plasmid. Interestingly, we found 0.5-kb truncated avirulence gene similar to aurXacE3 on the right border of avrBs3 homolgs of pAG1 and pXAG81. Two hundred twenty five isolates were analyzed to find aurBS3 or tra gene homologs by Southern hybridization. The numbers of avrBs3 homolog varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to can conjugative plasmids (pXAG81 type), and thirty eight isolates appeared to carry non-conjugative plamids (pAGl type). This indicated that aurBs3 gene homologs might be spread by conjugation in X. axonopodis pv. glycines.

• Journal of Microbiology and Biotechnology
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• 제19권4호
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• pp.403-408
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• 2009

A number of bifidobacterial species of human origin were screened for the presence of cryptic plasmids. One strain, Bifidobacterium longum FI10564, harbored plasmids of approximately 2.2 kb, 3.6 kb, and 4.9 kb in size. The smallest plasmid, pFI2576(2,197 bp), was studied in detail and its complete nucleotide sequence was determined. Computer-assisted analysis of this novel plasmid(G+C content 62%) identified 9 putative open reading frames(orfs), 3 of which were shown to be probable genes. These putative genes are arranged in an operon-like structure, in which the overlapping orfs 1 and 2 encode putative Rep proteins and are highly homologous to the rep genes of the B. longum plasmid pMBI(1,847 bp). The mechanism of replication of pFI2576 was investigated using Southern blot analysis of whole cell lysates, with and without S1 nuclease treatment, and atomic force microscopy(AFM). The results indicate that pFI2576 is likely to use the theta mode of replication.

• Journal of Microbiology and Biotechnology
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• 제13권3호
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• pp.457-462
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• 2003

The complete nucleotide sequence of a plasmid, pMG1, isolated from Bifidobacterium longum MG1 has been determined. This plasmid, composed of 3,862 base pairs with 65.1% of G+C content. harbors two major open reading frames (ORF) encoding putative proteins of 29 kDa (ORF I) and 71 kDa (ORF II). ORF I showed relatively high amino acid sequence homology with replication proteins of other plasmids from Gr Im-positive and -negative bacteria. Upstream of ORF I, four sets of tandem repeat sequences resembling the iteron structure of related plasmids were found. S1 endonuclease treatment and Southern blot analysis revealed that pMG1 accumulates single-stranded DNA (ssDNA) intermediate, which indicate i the rolling circle replication (RCR) mechanism of this plasmid. Homology search indicated that ORF II encodes plasmid mobilization protein, and the presence of highly conserved oriT sequence in the upstream of this gene supported this assumption. RT-PCR showed that only ORF I is expressed in vivo. Based on these results, pMG 1 was exploited to construct a shuttle vector, pBES2. It was successfully transformed into Bifidobacterium and maintained stably.

• 미생물학회지
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• 제37권1호
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• pp.37-41
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• 2001

Pleurotus ostreatus에서는 10.2 kb와 7.2 kb의 미토콘드리아 선상 플라스미드 DNA가 존재함이 발견되었다. 이 플라스미드 DNA의 양쪽 5'말단에는 단백질이 공유 결합되어있다고 알려져 있다. 최근에 P.ostreatus의 10.2 kb 미토콘드리아 선상 플라스미드 DNA를 다른 곰팡이의 선상 플라스미드 DNA에 존재하는 open reading frame(ORF)와 비교 분석하여 Podospora anserina의 플라스미드 pAL2의 DNA polymerase 및 RNA polymerase 암호부위와 유사성이 있음이 확인되었다. 본 연구에서는 7.2 kb선상 DNA를 HindIII잘라서 얻은 2조각의 절편(4.7 kb, 2.3 kb)을 클로닝하고 염기 서열을 분석하였다. 클로닝된 7 kb 절편에는 3개의 ORF,즉 ORF1(2982 bp, 993 amino acids), ORF2(2703 bp, 900 amino acids), ORF3(771 bp, 256 amino acids)가 존재함을 확인하였다 또한, 이들의 ORF를 분석한 결과, DNA polymerase와 RNA polymerase 암호부위와 유사성 이 있음을 확인하였다.

• 한국전산구조공학회논문집
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• 제19권4호
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• pp.347-356
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• 2006

본 논문에서는 단면상의 화이버 요소를 사용하여 3차원 강구조물의 점진적인 소성화를 고려하는 실용적인 비선형 비탄성 해석방법을 개발하였다. 부재의 p-$\delta$, p-$\Delta$ 등의 기하비선형은 안정함수로 고려하였다. 잔류응력은 단면상에 있는 화이버 요소에 초기응력을 가하여 고려하였다. 각 하중 단계에서 탄성상태인 단면을 계산하여 축강성과 휨강성을 직접 결정함으로서 점진적인 소성화를 고려하였다. 각 화이버 요소의 응력 변화를 계산하여 변형률 반전효과를 고려하였다. 제안된 해석 방법은 3차원 강구조물의 실용적인 해석 및 설계에 유용하게 사용될 것이라 판단한다.

• Journal of Microbiology
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• 제40권2호
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• pp.140-145
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• 2002

The 5.8 kb pMMH1, rolling-circle replication (RCR) plasmid of the wild type soil Bacillus mesentericus was developed into a novel secretion vector system in Bacillus subtilis. The pMMHl turned out to have a replication origin and two open reading frames (ORFs) of the putative γ-GTP and type I signal peptidase (sipP). To characterize the regions necessary for plasmid stability and high copy number, five vectors (pPS, pPP, pEN, pMN, pME) were constructed by disruption or deletion of each region in pMMH1. Like pMMHl all constructed vectors were stable over 100 generations In a non-selective medium. Since pPS was the smallest (2.3 kb)of all, it was selected for the construction of a navel secretion vector, Using the $\alpha$-amylase promoter/signal sequence of B. subtilils the novel plasmid pJSN was constructed. When $\beta$-glucosidase was expressed using pJSN, we found $\beta$-glucosidase activity in the medium. This result strongly suggested that plasmid pJSN can be used for the production of bioactive peptides in B. subtilis.