• Food Science and Preservation
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• v.13 no.2
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• pp.138-143
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• 2006

Clarified apple juice was prepared using different clarification methods including centrifugation, ultrafiltration (UF), and combined treatment (CT). Effects of clarification methods as well as other qualify parameters were investigated Clarification was clearly improved with an increase in centrifugation speed and towering temperature. Especially, lowering the temperature led to a decrease in turbidity values at 5,000 rpm. The optimum condition for centrifugation process was $5^{\circ}C$ and 10,000 rpm, respectively. UF and CT were very effective to produce clarified apple juice. The optimum condition of UF process was $45^{\circ}C$ and 150 kPa considering flux and turbidity. L*-values were increased while a*-values were decreased significantly after clarification regardless of methods (P<0.05). Vitamin C was most retained in the clarified samples using CT.

• Structural Engineering and Mechanics
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• v.31 no.2
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• pp.163-180
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• 2009

In the design of tall reinforced concrete (R/C) buildings, the serviceability stiffness criteria in terms of maximum lateral displacement and inter-story drift must be satisfied to prevent large second-order P-delta effects. To accurately assess the lateral deflection and stiffness of tall R/C structures, cracked members in these structures need to be identified and their effective member flexural stiffness determined. In addition, the implementation of the geometric nonlinearity in the analysis can be significant for an accurate prediction of lateral deflection of the structure, particularly in the case of tall R/C building under lateral loading. It can therefore be important to consider the cracking effect together with the geometric nonlinearity in the analysis in order to obtain more accurate results. In the present study, a computer program based on the iterative procedure has been developed for the three dimensional analysis of reinforced concrete frames with cracked beam and column elements. Probability-based effective stiffness model is used for the effective flexural stiffness of a cracked member. In the analysis, the geometric nonlinearity due to the interaction of axial force and bending moment and the displacements of joints are also taken into account. The analytical procedure has been demonstrated through the application of R/C frame examples in which its accuracy and efficiency in comparison with experimental and other analytical results are verified. The effectiveness of the analytical procedure is also illustrated through a practical four story R/C frame example. The iterative procedure provides equally good and consistent prediction of lateral deflection and effective flexural member stiffness. The proposed analytical procedure is efficient from the viewpoints of computational effort and convergence rate.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1449-1459
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• 2009

Coptidis rhizoma (huanglian) is an herb that is widely used in traditional Chinese medicine that has recently been shown to possess anticancer activity. However, the molecular mechanism underlying the anticancer effects of this herb is poorly understood. In this study, we investigated the anticancer activity of a combination of CR extract and arsenic trioxide, as well as the apoptotic pathway associated with its mechanism of action in human lung cancer H157 cells. Combined treatment of H157 cells with CR extract and arsenic trioxide resulted in significant apoptotic death. In addition, combined treatment with CR extract and arsenic trioxide acted in concert to induce a loss of mitochondrial membrane potential (${\Delta}{\Psi}$), the release of cytochrome c from mitochondria, and an increase in the expression of pro-apoptotic p53 and Bax protein, which resulted in activation of caspases and apoptosis. CR extract combined with arsenic trioxide also increased the lipid peroxidation, mRNA expression of DR4 and DR5 and caspase-8 activity. These data indicate that combined treatment with CR extract and arsenic trioxide enhanced apoptotic cell death in H157 cells through diverse pathways, including mitochondrial dysfunction and death receptors, particularly DR4 and DR5. Thus, this treatment may be an effective from of chemotherapy.

• Journal of the Korean Geotechnical Society
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• v.23 no.6
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• pp.85-95
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• 2007

In this study, in order to propose the prediction method of the residual settlement of clayey ground improved by vertical drains, a series of numerical analyses for a model ground were carried out using the elasto-viscous consolidation model. And the effects of ground improvement conditions of the ratio of effective radii $(r_e/r_w)$, consolidation pressure $({\Delta}p)$ on normally consolidated state, and the OCR (overconsolidation ratio) on overconsolidated state to reduce the residual settlement in three-dimensional consolidation by vertical drains were investigated by performing a series of numerical analyses. Furthermore, based on the results of a series of numerical analyses for the model ground, the predicting method of the residual settlement of clayey ground with vertical drains and the determination method of the value of OCR required to control the residual settlement within an acceptable value are proposed.

• Journal of Life Science
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• v.24 no.5
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• pp.505-514
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• 2014

To examine the anti-obese activity of miscellaneous cereal grains, 80% ethanol extracts from eight selected miscellaneous cereal grains were compared for their cytotoxic effects on 3T3-L1 murine preadipocytes. The ethanol extract of proso millet exhibited the highest cytotoxicity. Further fractionation of the ethanol extract with methylene chloride, ethyl acetate, and n-butanol showed that the cytotoxicity of the ethanol extract was mainly partitioned into the butanol fraction. As compared with differentiated mature adipocytes, 3T3-L1 preadipocytes were more susceptible to the cyctotoxicity of the butanol fraction. When each organic solvent fraction (25 ${\mu}g/ml$) was added during the differentiation period for 6 days, the cell viability was not affected significantly except for the butanol fraction, but the intracellular lipid accumulation declined to a level of 81.5%~50.3% of the control. The Oil Red O staining data also demonstrated that the ethanol extract as well as the butanol fraction could inhibit the differentiation of 3T3-L1 preadipocytes into mature adipocytes. The presence of the butanol extract during the induced adipocytic differentiation also resulted in a significant reduction in the expression levels of critical adipogenesis mediators $(C/EBP{\alpha}$, $PPAR{\gamma}$, aP2, and LPL) to a barely detectable or undetectable level and the cells retained the fibroblast-like morphology of 3T3-L1. In 3T3-L1 cells, the cytotoxicity of the butanol fraction (50-100 ${\mu}g/ml$) was accompanied by mitochondrial membrane potential (${\Delta}{\psi}m$) loss, caspase-3 activation, and PARP degradation. Taken together, these results indicate that proso millet grains possess pro-apoptotic and anti-adipocytic activities toward adipocytes, which can be applicable to prevention of obesity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.2
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• pp.194-200
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• 2007

The purpose of this study was to observe the effects of fiber free control diet (CD), cellulose diet (CED) and pectin diet (PTD) on diet induced thermogenesis (DIT) in healthy Korean woman for 3 hours. The three test diets were as follows: CD (carbohydrate intake: 63.4% of energy, protein intake: 14.0% of energy and fat intake: 25.8% of energy), CED (carbohydrate intake: 62.5% of energy, protein intake: 14.0% of energy and fat intake: 26.1% of energy) and PTD (carbohydrate intake: 62.7% of energy, protein intake: 14.0% of energy and fat intake: 26.2 of energy). Groups were served 10 g of cellulose for CED and 10 g of pectin for PTD, respectively. DIT was measured at fasting state and at 30, 60, 90, 120, 150 and 180 min after consuming each diet. The mean age of all subjects was $22.3{\pm}1.9$ years. Body weight was $52.5{\pm}8.6$ kg and body mass index was $20.6{\pm}2.7kg/m^2$. Preprandial resting energy expenditure was $0.79{\pm}0.02kcal/min$ and postprandial DIT were $14.05{\pm}0.62%$ for CD, $9.33{\pm}0.62%$ for CED, $11.07{\pm}1.35%$ for PTD as a percentage of the energy load. DIT of CD was significantly higher than those of CED and PTD. There was no significant difference in postprandial change in body temperature after consuming each test diets and the sympathetic nervous system activity measured by heart rate was significantly higher in CD than CED and PTD (p<0.05). With this study, it can be concluded that CED and PTD have significantly lowered in DIT (p<0.05). We didn't show the correlation of the factors that relate in DIT; thus, further experiments on that matter should be followed.

• Journal of Life Science
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• v.29 no.3
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• pp.295-302
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• 2019

Liquiritigenin (LG) is a chiral flavonoid isolated from the roots of licorice. It exhibits multiple biological activities including anti-oxidant, anti-cancer, and anti-inflammatory effects. In particular though, the anti-cancer activity of LG in oral squamous cell carcinoma has yet to be elucidated, and LG-induced apoptosis in oral squamous cell carcinoma remains poorly understood. In the present study, we tested the role of LG in inducing apoptosis in oral squamous cell carcinoma cells. LG treatment of HN22 cells resulted in a dose-dependent inhibition of cell viability as detected by a 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. The induction of apoptosis in terms of Annexin V/7-Aminoactinomycin D staining, sub-G1 population, and multi-caspase activity were assessed with a $Muse^{TM}$ Cell Analyzer. Flow cytometric analysis revealed that LG treatment resulted in G2/M arrest in cell cycle progression and downregulation of cyclin B1 and CDC2 expression in a concentration-dependent manner. It also resulted in significant upregulation of p27. In addition, LG was seen to trigger the generation of reactive oxygen species and induce CCAAT/enhancer-binding protein homologous protein and 78-kDa glucose-regulated protein in concentration-dependent upregulation. The LG treatment of HN22 cells led to a loss of mitochondrial membrane potential (${\Delta}{\Psi}m$); it also reduced the levels of anti-apoptotic protein and increased the expression of apoptotic protease activating factor-1, cleaved poly (ADP-ribose)polymerase and Bax. Overall, our results indicate that the pro-apoptotic effects of LG in HN22 cells depend on the activation of both intrinsic and extrinsic signaling pathways. Thus, our results suggest that LG constitutes a natural compound with a potential role as an anti-tumor agent in oral squamous cell carcinoma.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.362-370
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• 2019

This study was conducted to investigate effects of 12 weeks of combined exercise training with blood flow restriction (BFR) on body composition (weight, %body fat, lean body mass, body mass index (BMI)) and cardiovascular responses (brachial-ankle pulse wave velocity (ba PWV) and ankle-brachial index (ABI)) in elderly women. Participants (N = 43, Females) were randomly assigned into a combined exercise with BFR (n = 14, BFR), only combined exercise (n =14, EX) or non-exercise control group (n = 15, CON). Two-way repeated measures ANOVA with contrast testing was utilized for data analysis. Alpha was set at p < 0.05. Body composition (weight, %body fat, BMI) in BFR was significantly changed, and %body fat in EX was significantly decreased, but there was no change in the CON. In addition, the right and left ba PWV values in the BFR were significantly decreased, while only the left side ba PWV in EX was significantly decreased and there was no change in the CON. Moreover, the % change and effect size of most variables in the BFR were higher than the EX. Taken together, the results indicate that even though BFR and EX groups performed the same combined exercise training, BFR had additional stimulations of the sympathetic nerve system due to blood flow restriction. Thus, BFR training is more beneficial and has greater effects on body composition and cardiovascular responses in elderly females.

• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.598-604
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• 2013

This study was designed to evaluate the usability of the following citrus peel extracts (CPEs): Citrus sinensis (orange), C. unshiu (mandarin orange), C. limon (lemon), and C. paradise (grapefruit) as natural antibrowning agents. Overall, 0.1% of the CPEs were effective in reducing the browning of apple slices. The appearance of apple slices dipped in C. limon peel extracts (LPE) were found to be excellent and their Hunter L and ${\Delta}E$ values were similar to the values obtained when apple slices were dipped in ascorbic acid. The pH values of the different CPEs were not significantly different from each other, but were higher than that of ascorbic acid. LPE was found to possess the highest total phenolic content, flavonoid content, DPPH radical scavenging activity, and copper ($Cu^{2+}$) chelating activity. All these results suggest that citrus peel extracts, especially lemon peel extract, can be used as natural antibrowning agents.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.5
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• pp.629-634
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• 2006

Clarified mixed fruit and vegetable (apple, carrot and orange) juices were prepared using different clarification methods including centrifugation, ultrafiltration (UF), and combined treatment with the blend ratio of 1:1:2, 1:2:1, and 2:1:1 (apple, orange and carrot). Effects of clarification methods on the clarification efficacy as well as other quality parameters were investigated. Clarification was improved with increase in centrifugation speed but was less affected by lowering temperature. Ultrafiltration process was very effective to produce clarified mixed fruit and vegetable juice. Combined treatment did not significantly improve the clarification efficacy since most clarification was already achieved during ultrafiltration process (p>0.05). L*- and b*-values increased while a*-value decreased significantly after clarification regardless of methods in all blend juices (p<0.05). Blend juices with high amount of orange or carrot were better in clarification efficacy and high amount of apple resulted in high turbidity in blend samples.