• BMB Reports
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• v.47 no.7
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• pp.411-416
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• 2014

In the present study, we demonstrate that ectopic expression of 56-kDa human selenium binding protein-1 (hSP56) in PC-3 cells that do not normally express hSP56 results in a marked inhibition of cell growth in vitro and in vivo. Down-regulation of hSP56 in LNCaP cells that normally express hSP56 results in enhanced anchorage-independent growth. PC-3 cells expressing hSP56 exhibit a significant reduction of hypoxia inducible protein (HIF)-$1{\alpha}$ protein levels under hypoxic conditions without altering HIF-$1{\alpha}$ mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays a critical role in prostate cells by mechanisms including negative regulation of HIF-$1{\alpha}$, thus identifying hSP56 as a candidate anti-oncogene product.

• Korean Journal of Applied Biomechanics
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• v.29 no.2
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• pp.97-104
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• 2019

Objective: The aim of this study was to investigate the strategies for avoiding moving and stationary walker using body segments during walking. Method: Ten healthy young adults (10 males, age: $24.40{\pm}0.49yrs$, height: $175.80{\pm}5.22cm$, body mass: $70.30{\pm}5.22kg$) participated in this study. Each participant was asked to perform a task to avoid collisions with another walker who was moving or stationary during walking on the 10 m walkway. Both walkers were performed at natural self-selected walking speed. Results: Medio-lateral avoidance displacement of the trunk and the pelvis were significantly increased when avoiding a stationary walker (p<.05). There were no significant differences in medio-lateral center of mass trajectory. Rotation angle of trunk, pelvis and foot on the vertical axis were significantly increased when avoiding a stationary walker (p<.05). Conclusion: Based on our results, when another walker moves continuously, the walker recognizes another walker as the object of social interaction and performs the avoidance strategies while expecting the cooperative distance. On the other hand, when another walker is stopped, it is determined that the walker has an obligation to avoid, and the walker performs a relatively safer avoidance strategy.

• Applied Microscopy
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• v.50
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• pp.17.1-17.9
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• 2020

Recent advances in fabrication have enabled radial-junction architectures for cost-effective and high-performance optoelectronic devices. Unlike a planar PN junction, a radial-junction geometry maximizes the optical interaction in the three-dimensional (3D) structures, while effectively extracting the generated carriers via the conformal PN junction. In this paper, we report characterizations of radial PN junctions that consist of p-type Si micropillars created by deep reactive-ion etching (DRIE) and an n-type layer formed by phosphorus gas diffusion. We use electron-beam induced current (EBIC) microscopy to access the 3D junction profile from the sidewall of the pillars. Our EBIC images reveal uniform PN junctions conformally constructed on the 3D pillar array. Based on Monte-Carlo simulations and EBIC modeling, we estimate local carrier separation/collection efficiency that reflects the quality of the PN junction. We find the EBIC efficiency of the pillar array increases with the incident electron beam energy, consistent with the EBIC behaviors observed in a high-quality planar PN junction. The magnitude of the EBIC efficiency of our pillar array is about 70% at 10 kV, slightly lower than that of the planar device (≈ 81%). We suggest that this reduction could be attributed to the unpassivated pillar surface and the unintended recombination centers in the pillar cores introduced during the DRIE processes. Our results support that the depth-dependent EBIC approach is ideally suitable for evaluating PN junctions formed on micro/nanostructured semiconductors with various geometry.

• Journal of the Korean Chemical Society
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• v.29 no.5
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• pp.522-532
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• 1985

The reactions of p-nitrophenyldiphenylphosphate (p-NPDPP) with anions of benzimidazole (BI) and its 2-alkyl derivatives (R-BI) are strongly catalyzed by the micelles of cetyltrimethyl ammonium bromide (CTABr). On the other hand, the first order rate constants $(k'_{R-BI^-})$ and the second order rate constants $(k_{m(R-BI^-)})$ of the reactions mediated by R-$BI^-$in the micellar pseudophase are much smaller than those mediated by $BI^-$. In order to explain the slower rates of the micellar reactions mediated by R-$BI^-$, we compared the concentration-ratios ([R-$BI^-$]/[$BI^-$]) with the first order rate constant-ratios $(k'_{R-BI^-}/k'_{BI^-})$ and the second order constant-ratios $(k_{m(R-BI^-)}/k_{m(BI^-)})$ for the reactions taking place in the micellar pseudophase. The rate constant-ratios were much smaller than the concentration-ratios. For example in a 5 ${\times}10^{-4}$M butyl-BI solution, the two ratios were 0.089 and 0.430 (for the first order) respectively, and in a $10^{-4}$M butyl-BI solution the former was 0.100 (for the second order). This predicts that the reactivities of R-$BI^-$ in the micellar pseudophase are much smaller than that of $BI^-$. Based on the values of several kinetic parameters measured for dephosphorylation of p-NPDPP mediated by R-$BI^-$, a schemetic model is proposed. Due to the hydrophobicity and the steric effect of the alkyl substituents, these groups would penetrate into the core of the micelle for stabilization by van der Waals interaction with long cetyl groups of CTABr. Consequently, the movements of R-$BI^-$ bound to the micelle should be restricted, leading to decreased collison frequencies between the nucleophiles and p-NPDPP. We refer this as an "anchor effect". This effect became more predominent when a larger alky group in R-BI was employed and when a greater concentration of R-BI was used.

• The Journal of Korean Academic Society of Nursing Education
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• v.19 no.2
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• pp.183-193
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• 2013

Purpose: The purpose of this study aimed to describe the empathy and cultural competence and to find the relationship between the empathy and cultural competence in Korean nursing students. Methods: A total of 276 nursing students participated in this study. Data collection was conducted through the use of questionnaires which were constructed to include an Interpersonal Reaction Index, the Caffrey Cultural Competence in Healthcare scale, and Cultural Competence Assessment. Results: Empathy and cultural competency of participants in this study were shown to have moderate levels (Empathy: M=3.5, SD=0.41; Cultural competency: M=2.7, SD=0.42). And the nursing students who exhibited more frequent interaction with foreigners and willing to have multicultural education were found to have significantly higher empathy and cultural competence. There was a significant positive correlation between empathy and cultural competency(r=.189, p<.001). Conclusion: The findings suggest that nursing students need to enhance empathy and cultural competence.

• Ocean Systems Engineering
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• v.10 no.3
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• pp.317-332
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• 2020

This study examines the behaviors and properties of discharged liquid CO₂ from a long elastic pipe moving with a vessel for the oceanic CO₂ sequestration by considering pipe dynamics and vessel motions. The coupled vessel-pipe dynamic analysis for a typical configuration is done in the frequency and time domain using the ORCAFLEX program. The system's characteristics, such as vessel RAOs and pipe-axial-velocity transfer function, are identified by applying a broadband white noise wave spectrum to the vessel-pipe dynamic system. The frequency shift of the vessel's RAO due to the encounter-frequency effect is also investigated through the system identification method. Additionally, the time histories of the tip-of-pipe velocities, along with the corresponding discharged droplet size and Weber numbers, are generated for two different sea states. The comparison between the stiff non-oscillating pipe with the flexible oscillating pipe shows the effect of the vessel and pipe dynamics to the discharged CO₂ droplet size and Weber number. The pipe's axial-mode resonance is the leading cause of the fluctuation of the discharged CO₂ properties. The significant variation of the discharged CO₂ properties observed in this study shows the importance of considering the vessel-pipe motions when designing oceanic CO₂ sequestration strategy, including suitable sequestration locations, discharge rate, towing speed, and sea states.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.7
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• pp.927-936
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• 1998

In the fully developed pipe flow, when jet is injected in cross to the flow there are complex transition flows caused by interaction of the cross flow and jet. These interactions are studied by means of the flow visualization methods and frequency analysis using a hot-wire anemometer. The velocity range of cross flow of the pipe is 0.3 m/s ~ 1.2 m/s and the corresponding Reynolds number, R$\sub$p/, based on the pipe diameter is 2.25 * 10$\^$3/ ~ 9.02 * 10$\^$3/. The velocity ratio (R), jet velocity/cross flow velocity, is chosen from 2 to 10. A circular cylinder is placed in the pipe instead of jet to observe the vortex shedding from the solid body. To compare the jet and circular cylinder flow, the vortical structure is analyzed in both cases and the structure of vortices and the origin of its formation are investigated, especially. The vortex shedding of the dominant coherent structure is compared between the jet flow and the circular cylinder flow. In the case of the jet flow, the Strouhal numbers are different depending on the existence of the upright vortex as well as the velocity ratio (R).

• Bulletin of the Korean Chemical Society
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• v.29 no.2
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• pp.335-338
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• 2008

New efficient red emitter having short p?-conjugation length and asymmetric bulky structure, 2-(7-diphenylamino-benzo[1,2,5]thiadiazole-4-ylmethylene)-malononitrile, was synthesized and characterized. Using this material as a dopant, we fabricated electroluminescence device with a structure of ITO/DNTPD/NPD/BTZA (5 wt% in Alq3)/Alq3/LiF/Al. The device exhibited a high brightness of 761 cd/m2 at a driving voltage of 4.8 V, and current efficiency is 0.75 cd/A. The Commission International de IEclairage (CIE) coordinates of the EL device were found to be (0.62, 0.37) at 10 mA/cm2.

• Bulletin of the Korean Chemical Society
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• v.14 no.5
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• pp.574-578
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• 1993

Laser-induced photoacoustic spectroscopy (LIPAS), which utilizes the photothermal effect that results from nonradiative relaxation of excited state molecules, was used in the speciation analysis of the complexes of neodymium(III) and water soluble synthetic polyelectrolyte, poly methacrylic acid (PMAA), in 0.1 M $NaClO_4$ at pH of 6.0. The minimum detection limit of Nd(III) by LIPAS was $5.O{\times}10^{-6}$ M. Experiment was carried out at low concentration ratio of Nd(III) to PMAA to assure that 1 : 1 complexes predominate. The bound and free Nd(III) species were characterized by measuring nonradiative relaxation energy of the excited states $(^2GM{7/2}\;and\;^4G_{5/2})$ to the metastable state $(^4G_{3/2})$. Two species were quantified by deconvolution of the mixed spectrum using their respective reference spectra. The conditional stability constant measured by LIPAS was 5.52 L$mol^{-1}$.

• Bulletin of the Korean Chemical Society
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• v.6 no.5
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• pp.312-317
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• 1985

${\beta}$-Glucuronidase (EC 3.2.1.31) which hydrolizes D-glucuronate from ${\beta}$-D-glucuronide was purified from rat liver, using ammonium sulfate fractionation, DEAE-cellulose chromatography, Concanavalin-A Sepharose 4B chromatography and gel filtration on Sephadex G-200. This enzyme has the molecular weight of 280,000 daltons by gel filtration and 75,000 daltons by SDS-polyacrylamide gel electrophoresis. As its funtion is reverse of detoxification in the liver, the inhibition of the enzyme was tested with extracts of several food products and medicinal herbs, some are known as anti-cancer agents. Among them, Panax ginseng and Cortnellus shiiake inhibited the enzyme competitively and the $K_1$ values were $9.22 {\times}\;10^{-2}$ and 0.102 mg/ml, respectively. These inhibitors strongly bound to DEAE-cellulose. The negatively charged amino acids, L-aspartate and L-glutamate, inhibited the enzyme, and $K_1$ value of L-aspartate was 0.80 mM. The interaction between ${\beta}$-glucuronidase and p-nitrophenyl-${\beta}$-D-glucuronide was found to involve ionic forces by the effect of ionic strength on the kinetic constant, Vmax/Km. It was inferred from these findings that cationic group at the active center of the enzyme is probably involved in attacking the substrate.