• Title/Summary/Keyword: P-E

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Activity of virE promoter in Agrobacterium tumefaciens A348 (Agrobacterium tumefaciens A348에서 virE 프로모터의 활성)

  • 음진성
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.331-339
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    • 1991
  • To elucidate the regulatory mechanism of virE operon from vir regions (virA, virB, virC, virD, virG, virE) of pTiA6 which have been known to be essential for efficient crown gall tumorigenesis in plants, the activity of the truncated virE, promoter was analyzed. pSM358cd, a recombinant plasmid in which virE :: Tn3-HoHo1 (Tn3-promoterless lacZ) was cloned into SalI site of pVK102, was digested with SalI, and virE :: Tn3-HoHo1 was seperated from pVK102. To construct the truncted virE recombinant plasmids (pJS031, pJS051, pJS102, pJS201, pJS301), 5'-end of vireE promoter was deleted with BAL31 and cloned into pVK102 and then transferred into a. tumefaciens A348(pTiA6). According to the activity of the truncated virE promoter in recombinant plasmids, they were classified into two groups, pJS031, pJS051, pJS101 and pJS201 belong to a functional group and pJS301 is a non-functional. The size of deleted nucleotides of pJS201 and pJS301 seemed to be about 130 nucleotides and about 250 nucleotides from 5'-end of virE promoter, respectively. Hence it was thought that the essential site of the virE promoter was located between about 130th nucleotide and 250th nucleotide from 5'-end of the virE promoter.

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Effects of Removing of Transparent Polyethylene Film on Garlic Growth, Yield and Weed Occurrence in double Layer mulching Cultivation (이중피복 마늘재배 시 투명P.E.필름 제거가 마늘 생육 및 수량과 잡초 발생에 미치는 영향)

  • Lee, Jae-Sun;Kim, In-Jae;Youn, Cheol-Ku;Ahn, Ki-Su;Kim, Ki-Hyen;Nam, Sang-Yong;Kim, Hong-Sig
    • Korean Journal of Organic Agriculture
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    • v.21 no.3
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    • pp.413-422
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    • 2013
  • This experiment was conducted to clarify the effect of double layer mulching on reducing the labor required to weed control and Leaf sheath training of the garlic cultivation. Six mulching methods(non-mulching, transparent P.E., rice hull+transparent P.E., sawdust+transparent P.E., rice straw+transparent P.E., black P.E. film+ transparent P.E.) were used for the experiment, and transparent P.E film was removed on April 10. Weed occurrence was in the order of black P.E. film< transparent P.E

Effect of Mulching Materials on Growth and Agronomic Characteristics of Angelica gigas in Southern Area (남부지방에서 피복재료가 참당귀(Angelica gigas Nakai)의 생육과 주요 형질에 미치는 영향)

  • 윤혜경;최성규;이종일;윤경원;서영남;천상욱
    • Korean Journal of Plant Resources
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    • v.13 no.1
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    • pp.11-17
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    • 2000
  • In an effort to produce high quality Angelica gigas in the southern area of Korea, a variety of mulching materials were used to prevent withering during the summer. The experimental results are summarized as follows. Soil water content and temperature under various mulching materials were higher than those without mulching, in all the cases studied. Among various mulching materials P. E.(polyethylene) mulching material showed the highest effect. The appearance of weeds was the lowest when using the black P. E. mulching, while growth of weeds was the greatest when using transparent mulching. Growth and development of the plant were better with P. E. mulching than straw mulching or no mulching, allowed for more and bigger leaves. The bolting rate was about 2∼5%, with a slightly larger value under P. E. mulching. Under P. E. mulching, probably due to good physical properties of the soil the number of roots was large, and the length and diameter were good. Active growth of roots under P. E. mulching resulted in a yield increase of the plant. The black P. E. mulching material showed a prominent effect on suppression of weed growth, and the black P. E. material was considered to be the most recommendable among various P. E. mulching materials when there is a lack of labor.

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EXTREMUM PROPERTIES OF DUAL Lp-CENTROID BODY AND Lp-JOHN ELLIPSOID

  • Ma, Tong-Yi
    • Bulletin of the Korean Mathematical Society
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    • v.49 no.3
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    • pp.465-479
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    • 2012
  • For $0<p{\leq}{\infty}$ and a convex body $K$ in $\mathbb{R}^n$, Lutwak, Yang and Zhang defined the concept of dual $L_p$-centroid body ${\Gamma}_{-p}K$ and $L_p$-John ellipsoid $E_pK$. In this paper, we prove the following two results: (i) For any origin-symmetric convex body $K$, there exist an ellipsoid $E$ and a parallelotope $P$ such that for $1{\leq}p{\leq}2$ and $0<q{\leq}{\infty}$, $E_qE{\supseteq}{\Gamma}_{-p}K{\supseteq}(nc_{n-2,p})^{-\frac{1}{p}}E_qP$ and $V(E)=V(K)=V(P)$; For $2{\leq}p{\leq}{\infty}$ and $0<q{\leq}{\infty}$, $2^{-1}{\omega_n}^{\frac{1}{n}}E_qE{\subseteq}{\Gamma}_{-p}K{\subseteq}{2\omega_n}^{-\frac{1}{n}}(nc_{n-2,p})^{-\frac{1}{p}}E_qP$ and $V(E)=V(K)=V(P)$. (ii) For any convex body $K$ whose John point is at the origin, there exists a simplex $T$ such that for $1{\leq}p{\leq}{\infty}$ and $0<q{\leq}{\infty}$, ${\alpha}n(nc_{n-2,p})^{-\frac{1}{p}}E_qT{\supseteq}{\Gamma}_{-p}K{\supseteq}(nc_{n-2,p})^{-\frac{1}{p}}E_qT$ and $V(K)=V(T)$.

Transcription, Translation, and Immunolocalization of ODVP-6E/ODV-E56 and p74 Proteins: Two Highly Conserved ODV-associated Envelope Proteins of Choristoneura fumiferana Granulovirus

  • Rashidan, Kianoush Khajeh;Nassoury, Nasha;Giannopoulos, Paresa N.;Guertin, Claude
    • BMB Reports
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    • v.38 no.1
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    • pp.65-70
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    • 2005
  • Choristoneura fumiferana granulovirus (ChfuGV) infection results two types of enveloped virions: Occlusion-derived virus (ODV) and budded virus (BV). Structural proteins ODVP-6E/ODV-E56 and p74 are two major conserved ODV-associated proteins that may be involved in the initiation of viral infection cycle in susceptible host insect larvae. This study presents the characterization of ChfuGV odvp-6e/odv-e56 and p74 transcription and translation as well as immunolocalization of these proteins in the occluded ChfuGV virion. Our results revealed that the transcription of odvp-6e/odv-e56 and p74 genes, both, start at 24 hours post infection (h p.i.). Using monospecific polyclonal antibodies made against ODVP-6E/ODV-E56 and p74 we demonstrated that these proteins are both expressed late in infection (24 h p.i.). Immunogold labeling using antisera against ODVP-6E/ODV-E56 and p74 proteins demonstrated that ODVP-6E/ODV-E56 and p74 proteins are both associated with the ODV envelop of ChfuGV.

Effect of Mulching Materials on Growth and Agronomic Characteristics of Angelica gigas NAKA in Southern Area (남부 지방에서 피복 재료가 참당귀(Angelica gigas NAKAI)의 생육과 주요 형질에 미치는 영향)

  • 윤혜경;최성규;이종일;윤경원;서영남
    • Korean Journal of Plant Resources
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    • v.13 no.2
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    • pp.124-130
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    • 2000
  • In an effort to produce high quality Angelica gigas NAKAI in the southern area of Korea, a variety of mulching materials were used to prevent withering during the summer. The experimental results are summarized as follows. Soil water content and temperature under various mulching materials were higher than those without mulching, in all the cases studied. Among various mulching materials P. E.(polyethylene) mulching material showed the highest effect. The appearance of weeds was the lowest when using the black P. E. mulching, while growth of weeds was the greatest when using transparent mulching. Growth and development of the plant were better with P. E. mulching than straw mulching or no mulching, allowed for more and bigger leaves. The bolting rate was about 2∼5%, with a slightly larger value under P. E. mulching. Under P. E. mulching, probably due to good physical properties of the soil the number of roots was large, and the length and diameter were good. Active growth of roots under P. E. mulching resulted in a yield increase of the plant. The black P. E. mulching material showed a prominent effect on suppression of weed growth, and the black P. E. material was considered to be the most recommendable among various P. E. mulching materials when there is a lack of labor.

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The Influence of Materials for Surface Mulching on Soil Temperature and Vegetative Growth of Apple Nursery Trees (지표면 멀칭재료가 지온과 사과나무 묘목의 수체생육에 미치는 영향)

  • SaGong, Dong-Hoon;Lee, Su-Jin;Han, Su-Gon;Yoon, Tae-Myung
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.13 no.1
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    • pp.1-9
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    • 2011
  • This study was conducted to investigate the influence of different polyethylene film (P.E. film) for mulching on the changes in soil temperature and the production of good feathered apple nursery trees. M.9 rootstocks with stem diameter of 9.1~11.0 mm were planted in plots covered with different P.E. film (i.e., transparent P.E. film, black P.E. film, and bare soil as control). Three weeks after planting, the rootstocks were veneer grafted with 'Sinano Sweet' apple cultivar. In the middle of June, BA was sprayed to nursery for inducing feathering during the growing season. The soil temperature of the control was higher than air temperature by about $0.7^{\circ}C$ from mid April to early October, and that of P.E. film mulching was about $1{\sim}5^{\circ}C$ higher than that of the control. The soil temperature under transparent P.E. film was about $2{\sim}3^{\circ}C$ higher than that under black P.E. film. The diurnal range of soil temperature under the black P. E. film was lowest among all treatments. The P.E. film mulching induced better tree growth and feathering than bare soil. Percentage of good feathering apple nursery of black P.E. film was highest among all treatments because the soil temperature unuder black P.E. film in the early growing season was higher than that of the control and the number of days when the maximum soil temperature was over $35^{\circ}C$ in the summer was lower than that under the transparent P.E. film.

Prognostic Significance of Cyclin E and p27 in Stage 1 Non-Small Cell Lung Cancer (제1병기 비소세포폐암에서 Cyclin E와 p27의 발현과 예후)

  • 조봉균;조성래;천봉권
    • Journal of Chest Surgery
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    • v.36 no.1
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    • pp.7-14
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    • 2003
  • Cyclin I plays a pivotal role in the regulation of G1-S transition and could consequently be a deregulated molecule in tumors. The activity of the cdk2-cyclin E complex is increased by degradation of cdk inhibitor p27kip1. Little is known about the expression and prognostic significance of cyclin E and p27 in non-small cell lung cancer(NSCLC). Material and Method: The expression of cyclin E and p27 in eighty-one cases of resected stage I NSCLC tissues and its relation to major clinico-pathological factors, including histology, differentiation, size of tumor, pleural invasion and survival rate were studied and analyzed. Immunohistochemical analysis with monoclonal antibodies specific for cyclin E and p27 were performed by ABC method. Result: Expression rates of cyclin E and p27 in stage I NSCLC tissues were 29.6% and 28.4% respectively. Cyclin E was expressed higher in cases of pleural invasion(p=0.04), and p27 was expressed higher in diameter of tumor less than 3cm(p=0.015). The 5-years survival rate was lower in cases of Positive expression of cyclin E than in cases of negative expression of cyclin E(44.4% vs 68.2%, p=0.015), and the 5-years survival rate was 72.2% in positive expression of p27 and 56.2% in negative expression of p27(p=0.09). The 5-years survival rate was higher in negative expression of cyclin E and positive expression of p27 than in cases of positive expression of cyclin I and negative expression of p27 (73.5% vs 36.3%, p=0.0029). In multivariate analysis, expression of cyclin I was an unfavorable prognostic factor(RR=3.578, p=0.006) and p27 was a favorable prognostic factor(RR=0.183, p=0.019) independently. Conclusion: Cyclin E and p27 may play a pivotal role for the biological behavior of stage I NSCLC, so that the expressions of cyclin I and p27 nay be new prognostic markers.

Kinematic Variables Comparison of Setter Toss Motion on Volleyball According to Toss Types (배구경기 세터 토스 동작의 운동학적 비교분석)

  • Chung, Nam-Ju;Kim, Jae-Pil
    • Korean Journal of Applied Biomechanics
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    • v.25 no.1
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    • pp.57-64
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    • 2015
  • Purpose : The purpose of this study was to analyze setter toss motion kinematically according to toss types. Method : Dependent variables were elapsed time, vertical displacement of the body center, the projected speed of the ball, and differences of the joint angle to the target for four setters positioning. Result : There was no significant difference in the time but the ball contact time was shorter when the toss distance of P3 was longer. There was significant difference in the vertical displacement of COM (p<.05). The vertical displacement of COM showed that the vertical movement gradually decreased when the quick distance was longer. The vertical displacement of COM was difference (p<.05), also there was difference of the ball speed (p<.001) at the Release point(E4). There was significant difference in the knee joint angle at a certain moment among the Release(E4) and Landing point(E5)(p<.05). The hip joint was significant difference among the Apex(E2), Ball Touch(E3), Release(E4), and the Landing point(E5) on the surface(E2, E3, E4 p<.05; E5 p<.005). The shoulder angle was significant difference among the Ball Touch(E3), Release(E4) and the Landing point(E5) on the surface(E3, E4 p<.05; E5 p<.001). The elbow was significant difference in the Apex(E2) (p<.05). The wrist was significant difference in the Release(E4) (p<.05). Conclusion : If we find the clue to expect the direction of the setter's ball, we have to fine the clues in the Apex(E2) that hip join and elbow, Ball Touch(E3) that hip joint and shoulder joint, Release(E4) that wrist, elbow, hip joint, and knee joint.

Seasonal Distribution of Marine Cladocerans in Chinhae Bay, Korea (진해만 해산 지각류의 계절적인 소장)

  • 유광일;김세화
    • 한국해양학회지
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    • v.22 no.2
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    • pp.80-86
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    • 1987
  • Five species of marine cladocerans, Evadne nordmanni, Evadne tergestina, Podon leuckarti, Podon polyphemoides and Penilia avirostris, were found in Chinhae Bay during the years 1982-1983. Incidence of E. nordmanni(April-May) in spring and of E. tergestina(June-December), E. avirostris(June-November) in summer through autumn was noticed during the present study. But P. polyphem oides appeared throughout the year except February. P. avirostris was the most abundant species(21,491indiv./㎥), followed by P. polyphemoides and E. tergestina. Population of E.normanni and P. leuckarti was extremely poor and recorded to be less than 1,000indiv./㎥. Seasonal change in size composition was noticed in E. tergestina and P. avirostris. Increase of length was recorded in P. polyphemoides when water temperature lower than 15$^{\circ}C$.

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