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The Application of HACCP System to Soybean Curd and Its Effectiveness (두부류에 대한 HACCP 적용 및 성과)

  • Park, Wan-Hee;Lee, Sung-Hak
    • Journal of Food Hygiene and Safety
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    • v.18 no.4
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    • pp.202-210
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    • 2003
  • This study aims at making a HACCP(Hazard Analysis Critical Control Point)plan to be applied to soybean curd and verifing its effectiveness. First, we develped a general model of HACCP according to the guidelines of Codex (FAO/WHO). And we applied the model to 4 soybean curd workshops for 3 months. The HACCP model is composed of these procedures; HACCP team organization, production description, work flow chart, hazatd analysis, CCP (critical control point) decision, CL (critical limit) establishment, monitoring method decision, correction, verification and documentation. CCP were selection procedure and refrigeration procedure in non-wrapped soybean curd. CCP were selection procedure, heat-sterilizing and refrigeration in wrapped soybean curd. The result of bacterial experiment after apling the model for 3 months, the bacterial numbers of soybean curd box, wrapper, and soybean curd production were lower after appling than before appling, the model. We could verify that the appications of the HACCP model were effective to the soybean curd workshops.

Geochemical Properties of Deep Sea Sediment in the Benthic Environmental Impact Experiment Site (BIS) of Korea (심해 저층환경충격 시험지역의 퇴적물 지화학적 특성)

  • Kong, Gee Soo;Hyeong, Kiseong;Choi, Hun-Soo;Chi, Sang-Bum
    • Ocean and Polar Research
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    • v.36 no.4
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    • pp.407-421
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    • 2014
  • The benthic environmental impact experiment addresses environmental impacts at a specific site related to deep-sea mineral resource development. We have acquired several tens of multi- or box core samples at 31 sites within the Benthic environmental Impact Site (BIS) since 2010, aiming to examine the basic properties of surficial deep-sea sediment as a potential source for deep-water plumes. In this study, we present the geochemical properties such as major elements, rare earth elements (REEs), and heavy metal contents at the BIS. Such proxies vary distinctly according to the Facies association. The lithology of all core sediments in the BIS corresponds to both Association Ib and Association IIIb. The vertical profiles of some major elements ($SiO_2$, $Fe_2O_3$, CaO, $P_2O_5$, MgO, MnO) show noticeable differences between Association Ib and IIIb, while others ($Al_2O_3$, $TiO_2$, $Na_2O$, and $K_2O$) do not vary between Association Ib and IIIb. REEs are also distinctly different for Associations Ib and IIIb; in Association Ib, REY and HREE/LREE are uniform through the sediment section, while they increase downward in Association IIIb like the major elements; below a depth of 8 cm, REY is over 500 ppm. The metal enrichment factor (EF) evaluates the anthropogenic influences of some metals (Cu, Ni, Pb, Zn, and Cd) in marine sediments. In both Associations, the EF for Cu is over 1.5, the EF for Ni and Pb ranges from 0.5 to 1.5, and the EF for Zn and Cd are less than 0.5, indicating Cu is enriched but Zn and Cd are relatively depleted in the BIS. The vertical variations of geochemical properties between Association Ib and IIIb are shown to be clearly different, which seems to be related to the global climate changes such as the shift of Intertropical convergence zone (ITCZ).

A Study on the Perceptions of Food & Nutriton Section and Eating Behaviors of Middle School Students (중학생의 식생활 단원에 대한 인식과 식행동에 관한 연구)

  • 김경애;최현덕
    • Journal of Korean Home Economics Education Association
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    • v.11 no.2
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    • pp.89-110
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    • 1999
  • The purpose of this study was to investigate the perception and eating behaviors of middle school students in Kwangju area about food and nutrition section of Home Economics course. The subjects were 480 middle school students. The major results were as follows: On the perception f food and nutrition section, the 1st graders learned ‘how to settle the problems related to food and nutrition and food choices’. The 2nd graders learned ‘the knowledge and principle of food and nutrition for improving dietary life’. The 3rd graders learned ‘actual dietary life functions like arranging menu and preparing meals’. Consequently, there was a significant difference in each grade and all boy and girl students have the highest response to the item ‘they learn how to settle the nutrition problem and foods choices’. On the interests in food and nutrition section, lower graders and girl students have higher interest than hgiher graders and boy students. The area which was considered as most interesting in food and nutrition sections was ‘cooking’ in all students. The perception of the concern on dietary life after learning food and nutrition section was higher in the 1st graders. On the perception of the area where was a great change in their own dietary life after learning food and nutrition section, the 1st graders responded there was a great change in ‘balanced dietary life’, the 2nd graders in ‘eating habit and nutrition of adolescence’and the 3rd graders in ‘cooking’. Especially, girl students showed more changes. On the perception of degree which food and nutrition section gives a help to actual life, students over 50% responded that it was helpful to their actual life. This showed that the lower the grades, the more help it gave to those students. On the perception of mother’s job, the students with working mother considered that they learned ‘actual dietary life functions like functions like arranging menu and preparing meals’from the food and nutrition section. In addition, they had high interests in the area of ‘cooking’. It was recognized that as the family size was greater, the food and nutrition section gave more helps to actual life. On eating behaviors of breakfast, the higher the grade, the more frequent they don’t have breakfast, boy students have more frequencies of having breakfast than girl students and the primary reason of not having breakfast is poor appetite. On whether going without a meal affects study or not, most of the 1st, 2nd and 3rd graders whether they are boys and girls or not responded it affected their study. On eating behaviors of lunch, most of boy and girl students in the 1st, 2nd 3rd grade have lunch box. On eating behaviors of supper, eating time is usually between 7:00 and 8:00 p.m and eating hour is mostly 10 or 15 minutes. On eating behaviors between meals, frequency of eating between meals is mostly once or twice a day and girl students have more frequencies of eating between meals, and the cause was hunger commonly in boy and girl students and ‘killing time’in girl students. The focus in getting the foods is ‘taste’and favorite food is fruit regardless of the grade.

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Study on the Properties of $CuInSe_2$ absorber layer from Selenization using a closed Vacuum Quartz box (진공 석영관에서 Selenization한 $CuInSe_2$ 광흡수층 특성 연 구)

  • Yang, Hyeon-Hyn;Back, Su-Ung;Kim, Han-Wool;Han, Chang-Jun;Na, Kil-Ju;Lee, Suk-Ho;So, Soon-Youl;Park, Gye-Choon;Lee, Jin;Chung, Hae-Deok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.229-229
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    • 2010
  • 본 실험에서는 $CuInSe_2$, 3원물질을 화학량론적 조성비가 되도록 박막을 제조하기 위해 각 단위원소를 원자비에 맞춰 전자선가열 진공증착기를 사용하여 Cu, In, Se 순으로 증착하였다. $10^{-3}$torr 이상의 진공석영관에서 열처리와 동시에 Selenization을 통해 제작된 $CuInSe_2$박막은 열처리온도 $250^{\circ}C$에서는 $Cu_xSe$, CuSe등의 2차상들이 나타나다가 $450^{\circ}C$이상의 고온에서 $CuInSe_2$ 단일상을 형성하였다. 이로부터 진공중에서 반응을 시켰을 때, 더 낮은 온도에서 반응이 일어나고 열역학적으로 보다 안정한 소수의 화합물들이 쉽게 형성됨을 확인할 수 있었다. 특히 $250^{\circ}C$에서는 Sphalerite 구조를 가지다가 $350^{\circ}C$이상의 온도에서 Selenization하였을 때 Chalcopyrite 구조를 가졌다. 박막이 두꺼워지면서 결정립의 크기가 커지고 응력이 작아지는 특성을 보였다. 에너지 밴드갭은($E_g$)은 Cu/In 성분비율이 클수록 작은값을 보였으며, 결절립크기가 증대되므로 결국 흡수계수가 낮아짐을 알 수 있다. 또한 두께가 증가할수록 전반적으로 흡수계수가 증가하였고 Cu/In의 성분비율이 0.97일 때 기초흡수파장은 1,169nm이고 에너지밴드갭은 1.06eV이었으며, 두께 $1.5{\mu}m$이상일 때 전반적으로 양호한 상태의 p-type $CuInSe_2$ 박막을 제작 하였다.

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Evaluation of carbon flux in vegetative bay based on ecosystem production and CO2 exchange driven by coastal autotrophs

  • Kim, Ju-Hyoung;Kang, Eun Ju;Kim, Keunyong;Jeong, Hae Jin;Lee, Kitack;Edwards, Matthew S.;Park, Myung Gil;Lee, Byeong-Gweon;Kim, Kwang Young
    • ALGAE
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    • v.30 no.2
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    • pp.121-137
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    • 2015
  • Studies on carbon flux in the oceans have been highlighted in recent years due to increasing awareness about climate change, but the coastal ecosystem remains one of the unexplored fields in this regard. In this study, the dynamics of carbon flux in a vegetative coastal ecosystem were examined by an evaluation of net and gross ecosystem production (NEP and GEP) and $CO_2$ exchange rates (net ecosystem exchange, NEE). To estimate NEP and GEP, community production and respiration were measured along different habitat types (eelgrass and macroalgal beds, shallow and deep sedimentary, and deep rocky shore) at Gwangyang Bay, Korea from 20 June to 20 July 2007. Vegetative areas showed significantly higher ecosystem production than the other habitat types. Specifically, eelgrass beds had the highest daily GEP ($6.97{\pm}0.02g\;C\;m^{-2}\;d^{-1}$), with a large amount of biomass and high productivity of eelgrass, whereas the outer macroalgal vegetation had the lowest GEP ($0.97{\pm}0.04g\;C\;m^{-2}\;d^{-1}$). In addition, macroalgal vegetation showed the highest daily NEP ($3.31{\pm}0.45g\;C\;m^{-2}\;d^{-1}$) due to its highest P : R ratio (2.33). Furthermore, the eelgrass beds acted as a $CO_2$ sink through the air-seawater interface according to NEE data, with a carbon sink rate of $0.63mg\;C\;m^{-2}\;d^{-1}$. Overall, ecosystem production was found to be extremely high in the vegetated systems (eelgrass and macroalgal beds), which occupy a relatively small area compared to the unvegetated systems according to our conceptual diagram of a carbon-flux box model. These results indicate that the vegetative ecosystems showed significantly high capturing efficiency of inorganic carbon through coastal primary production.

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

  • Ropka-Molik, Katarzyna;Bereta, Anna;Zukowski, Kacper;Tyra, Miroslaw;Piorkowska, Katarzyna;Zak, Grzegorz;Oczkowicz, Maria
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1565-1574
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    • 2018
  • Objective: The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants' effect on histological microstructure and production traits in a larger pig population. Methods: RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results: Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin 'eye' area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism. Conclusion: This study's results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

Field Application of 80MPa High Strength Fire Resistant Concrete using Ternary Blended Cement (설계강도 80MPa 3성분계 고강도내화콘크리트의 현장적용 및 성과분석)

  • Kim, Seong-Deok;Kim, Sang-Yun;Bae, Ki-Sun;Park, Su-Hee;Lee, Bum-Sik
    • Journal of the Korea Institute of Building Construction
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    • v.10 no.5
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    • pp.113-119
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    • 2010
  • Fire resistance and field tests for high-strength concrete(HSC) of 80MPa were carried out to evaluate whether or not it shows the same material properties even in the field condition of being mass-produced and supplied. As a result, it was found that fire resistant HSCs containing composite fiber(NY, PP) of 0.075% have great resistance to fire and spalling. In the field test, before the pumping air contents, slump flow, U-box, L-flow, compressive strength, gap of hydration temperature of interior and exterior of specimen and placing ratio per hour satisfied the required properties of HSC. However, after the pumping of HSC, as slump flow and L-flow were slightly less than required criterion, they need to be improved. In terms of hydration temperature of HSC, it was found to satisfy the related criterion. Packing ability as well as placing ratio per hour of HSC, which was about $44m^3$, show outstanding results. If slump flow of developed ternary HSC is improved after the pumping it can be useful for the construction of high-rise buildings.

Variations of Physico-Mechanical Properties of the Cretaceous Mudstone in Haman, Gyeongnam due to Freeze-Thaw Weathering (경남 함안군 백악기 이암의 동결-융해에 따른 물성변화 및 미세균열 발현특성)

  • Um, Jeong-Gi;Shin, Mi-Kyoung
    • Tunnel and Underground Space
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    • v.19 no.2
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    • pp.146-157
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    • 2009
  • An experimental study of accelerated weathering on mudstone sample specimens from Haman, Gyeongnam was performed to investigate the variations of physico-mechanical properties of deteriorated rocks due to freeze-thaw weathering. Each complete cycle of freeze and thaw lasted 24 hours, comprising 2 hours of saturating in vacuum chamber, 8 hours of freezing at $-16{\pm}1^{\circ}C$ and 14 hours of thawing at room temperature. Total of 55 cycles of freeze-thaw were completed with measuring the index properties as well as geometries of microfractures. The measured specific gravity and P-wave velocity found to decrease with increasing freeze-thaw cycles. On the other hand, absorption ratio and effective porosity were continuously increased with increasing freeze-thaw cycles. It was found that the index properties of deteriorated sample specimen depend on its initial properties and flaws in rock. The size and density of the traces of the microfracture on slab specimen exhibited abrupt changes after 30 cycles of freeze-thaw weathering. The results obtained in this study show that the box fractal dimension($D_B$) given in this paper has the strong capability of quantifying the size and density of the microfracture.

Development of Differential Diagnosis and Treatment Method of Reproductive Disorders Using Ultrasonography in Cows II. Differential Diagnosis of Subestrous Dairy Cows (초음파검사에 의한 소의 번식장애 감별진단 및 치료법 개발 II. 무발정우의 감별진단)

  • 강병규;최한선;강현구;오기석;서동호;손창호;서국현
    • Journal of Veterinary Clinics
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    • v.15 no.2
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    • pp.307-318
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    • 1998
  • Accuracy of rectal palpation and ultrasonography for differential diagnosis of subestrous dairy trows were investigatedl using the result of pIRsma progesterone assay. The ovaries were examined 2 times of 10 days interval in 520 posearom and postinsemination subestroHs dairy cows, using rectal palpation and B-mode transrectal ultrasonography. The results of rectal palpation, ultrasonographic examination and measurement of plasma progesterone profiles in 520 subestrous dairy cows were silent brat or error of estrus detection 303 (58.3%), persistent corpus luteum 59 (11.3%), follicular cyst 37 (7.1%), luteal cyst 16 (3.1%), inactive ovary 9 (1.7%), granulosa tumor 1 (0.2%), hydmsalphinx 1 (0.2%), endomehris 81 (15.6%), pyometra 12 (2.3%) and mummified fetus 1 (0.2%), respectively. Accuncy of rectal palpation and ultrasonography for diagiosing ovarian disordeir based on plasma progesterone profiles were silent heat or error of estrus detection 80.5% and 96.7%$\boxUl$ persistent corpus luteum 57.6% and 94.9%, follicular cyst 62.5% and 91.9%1 luteal cyst 62.5% and 87.5%, maclive ovary 55.6% and 88.9% and granulosa cell tumor 100% and 100%, respectively. Acnuucy of rectal palpation for diagnosing uterine disorders based on ultrasonography was pyometra 75.0%1 endometritis 51.9% and mummified fetus 100%, respectively. Cbaracteristic ultrasonographic appearances of ovaries in subestrous dairy cows were as follows; Silent heat or error of estrus detection: anechoic follicle or hypoechoic corpus luteum than ovarian stroma was alternately present on Day 0 (first examination) and Day 10. Follicular cyst: uniformly nonechogenic ovarian structure $\geq $ 25 mm in diameter with a wall < 3 mm was present in ipsilateral on Day 0 and Day 10. Luteal cyst: luteal cyst was similar to follicular cysts but thickness of cystic wall was $\geq $ 3 mm. Inactive ovary : structures within ovaries was not present on Day 0 Bnd Day 10. Characteristic uthssonograpsc appearances of uterus in subestrous dairy cows were as follows; Endometritis: characterized by uterine lumen containing fluid in which 'snowy'echogenic particles art suspended. Pyometra: ultrasonographic appearance of pyometra was diffuse echogenic particles distributed in fluid within the distended uterus, and a thickened uterine wall. These results indicated that ultrasonography was practical far diagnosing reproductive disorders. To diagnosing ovarian disorders, ultrasonography should be carried out 2 times of 10 days interval and rndometritis should be differentiated with uterus of luteal phase in normal cycling cows.

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The Effects of Injinchunggan-tang(Yinchenqinggan-tang) on DMN-induced Liver Damage by Applying Proteomics (인진청간탕(茵蔯淸肝湯)이 DMN 유발 간섬유화와 단백질 발현에 미치는 영향)

  • Park, Sang-Baek;Kim, Young-Chul;Lee, Jang-Hoon;Woo, Hong-Jung
    • The Journal of Internal Korean Medicine
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    • v.29 no.1
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    • pp.200-218
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    • 2008
  • Objectives : The purpose of this study was to investigate the effects of Injinchunggan-tang (Yinchenchinggan-tang) on DMN-induced liver damage by applying proteomics. Materials and Methods : Sprague-Dawley rats were used in this experiment and were divided into the normal group (normal saline), the control group (DMN) and the sample group (DMN+IJCGT). DMN was injected i.p. once a day three times a week for 3 weeks in the control group. Normal saline instead of DMN was administered to the normal group. In the sample group, Injinchunggan-tang (Yinchenchinggan-tang) extract was orally administered once a day for 10 days after DMN was induced. The livers of each group were processed and analyzed by histology, Western blot, $Oxyblot^{TM}$, CBB and 2-dimensional electrophoresis. Results : In the histological findings of the liver, IJCGT reduced collagen deposition and liver damage in DMN-induced hepatic fibrosis. IJCGT increased MMP-13 protein production assessed by western blot. Protein oxidation induced by DMN treatment was decreased by IJCGT. In the 2-dimensional electrophoresis finding, the level of the increased proteins induced by DMN treatment such as GRP 75, 58kDa glucose regulated protein and heat shock 70kDa protein 5 were decreased by IJCGT. IJCGT was considered to have the protective effects on hepatotoxicity induced by DMN. In the 2-dimensional electrophoresis finding, the level of increased oxidized proteins such as heat shock 70 protein, mitochondrial malonyltransferase, calreticulin precursor, actin, NADP-isocitrate dehydrogenase, ankyrin repeat and SOCS box protein 11 were decreased by IJCGT. IJCGT was considered to have protective effect on the protein production induced by DMN treatment. Conclusion : Injinchunggan-tang (Yinchenchinggan-tang) exerts an inhibitory effect against the fibrosis and protein oxidation induced by DMN treatment in the rat liver. IJCGT was considered to have protective effects on the hepatotoxicity and protein production induced by DMN treatment.

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