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Inhibitory Effects of Ethaverine on Serotonin Content in Murine Mastocytoma P815 Cells (Ethaverine 화합물이 P815 세포중의 Serotonin 함량에 미치는 영향)

  • Kim, Eung-Il;Shin, Jung-Soo;Lim, Sung-Cil;Park, Seung-Kook;Lee, Myung-Koo
    • YAKHAK HOEJI
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    • v.51 no.2
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    • pp.83-87
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    • 2007
  • The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5${\sim}$10 ${\mu}$M decreased serotonin content in a concentration-dependent manner. The IC$_{50}$ value of ethaverine was 6.1 ${\mu}$M. Ethaverine at concentrations up to 20 ${\mu}$M was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5 ${\mu}$M), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.

Correlations of Sensory Quality Characteristics with Intramuscular Fat Content and Bundle Characteristics in Bovine Longissimus Thoracis Muscle

  • Choi, Young Min;Garcia, Lyda Guadalupe;Lee, Kichoon
    • Food Science of Animal Resources
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    • v.39 no.2
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    • pp.197-208
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    • 2019
  • The aim of this study was to investigate the relation of sensory quality traits of cooked beef to fresh meat quality and histochemical characteristics, especially muscle bundle traits, in the longissimus thoracis muscle of Hanwoo steers. Cooking loss negatively correlated with softness, initial tenderness, chewiness, rate of breakdown (RB), and amount of perceptible residue (AR) after chewing (p<0.05), and drip loss showed negative correlation with RB and AR (p<0.05). All the attributes of tenderness exhibited negative correlation with the Warner-Bratzler shear force value (p<0.05). Marbling score and the intramuscular fat (IMF) content showed positive correlation with all the organoleptic characteristics, including tenderness attributes, juiciness, and flavor (p<0.05). Regarding histochemical characteristics, muscle fiber size did not have a significant correlation with all the sensory quality traits, although the area percentage of type I fiber was related with softness, initial tenderness, and chewiness (p<0.05). On the contrary, the characteristics of muscle bundle were related to all the sensory tenderness attributes (p<0.05), and the sensory tenderness increased with smaller muscle bundle size (p<0.05). These results suggest that the IMF content and bundle characteristics can be used as indicators for explaining the variations in sensory tenderness in well-marbled beef.

Effect of barium silicate filler content on mechanical properties of resin nanoceramics for additive manufacturing

  • Won, Sun;Ko, Kyung-Ho;Park, Chan-Jin;Cho, Lee-Ra;Huh, Yoon-Hyuk
    • The Journal of Advanced Prosthodontics
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    • v.14 no.5
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    • pp.315-323
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    • 2022
  • PURPOSE. The purpose of this study was to investigate the effect of barium silicate filler contents on mechanical properties of resin nanoceramics (RNCs) for additive manufacturing (AM). MATERIALS AND METHODS. Additively manufactured RNC specimens were divided into 4 groups depending on the content of ceramic fillers and polymers: 0% barium silicate and 100% polymer (B0/P10, control group); 50% barium silicate and 50% polymer (B5/P5); 60% barium silicate and 40% polymer (B6/P4); 67% barium silicate and 33% polymer (B6.7/P3.3). The compressive strength (n = 15) and fracture toughness (n = 12) of the specimens were measured, and scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) analyses were performed. Independent sample Kruskal-Wallis tests were performed on the compressive strength and fracture toughness test results, and the significance of each group was analyzed at the 95% confidence interval through post-tests using the Bonferroni's method. RESULTS. B6/P4 and B6.7/P3.3 exhibited much higher yield strength than B0/P10 and B5/P5 (P < .05). Compared to the control group (B0/P10), the other three groups exhibited higher ultimate strength (P < .05). The fracture toughness of B6/P4 and B6.7/P3.3 were similar (P > .05). The content of barium silicate and fracture toughness showed a positive correlation coefficient (R = 0.582). SEM and EDS analyses revealed the presence of an oval-shaped ceramic aggregate in B6/P4 specimens, whereas the ceramic filler and polymer substrate were homogeneously mixed in B6.7/P3.3. CONCLUSION. Increasing the ceramic filler content improves the mechanical properties, but it can be accompanied by a decrease in the flowability and the homogeneity of the slurry.

Comparison of Antioxidant Potentials in Methanolic Extracts from Soybean and Rice Fermented with Monascus sp.

  • Pyo, Young-Hee
    • Food Science and Biotechnology
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    • v.16 no.3
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    • pp.451-456
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    • 2007
  • The potential antioxidant activities of methanolic extracts from soybean and rice fermented with Monascus sp. were investigated. M. pilosus IFO 480 and M. anka IFO 478 were screened as a suitable strain to promote the antioxidant activities in soybean- and rice- fermentation. The methanol extracts from soybean and rice after fermenting for 20 days at $30^{\circ}C$ resulted in a significant increase in the antioxidant capacities expressed as radical (ABTS and DPPH) scavenging assay and peroxidation inhibition (%) by thiocyanate method and increased (p<0.01) by a 2.6 to 3.1-fold compared with those of the unfermented products. The average antioxidant potentials of Monascus-fermented soybean extracts (MFSE) were significantly (p<0.01) stronger than Monascus-fermented rice extracts (MFRE). A linear correlations between free radical scavenging activity of MFSE and the total phenolics content (r=0.84) and total flavonoids content (r=0.81) were observed. These results indicated that MFSE exhibited stronger (p<0.01) antioxidant activity and contained significantly higher levels (p<0.05) of phenolics than MFRE.

Changes in pH, Acidity, Organic Acid and Sugar Content of Dough for Jeungpyun During Fermentation (발효과정 중 증편 반죽의 pH, 산도, 유기산 및 당함량의 변화)

  • Park, Young-Sun;Suh, Chung-Sik
    • Journal of the Korean Society of Food Culture
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    • v.9 no.4
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    • pp.329-333
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    • 1994
  • Physicochemical Properties of dough for Jeungpyun(Korean steamed rice cake) were analyzed during fermentation in the ranges of 0 to 10 hours. As fermentation time went by, pH of Jeungpyun dough was decreased while its titratable acidity and lactic acid content were increased continuously. Total sugar content was decreased continuously during fermentation. Reducing sugar content was increased greatly at the early stage of fermentation, reaching maximum value, 101 mg/g-dry matter at 4 hours, but it was decreased thereafter.

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Effects of Nitrogen Fertilization on Forage Production and Nutritive Nalue of Geukdong 6, Teosinte Hybrid [Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis]

  • Wang, Chengyu;Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.1
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    • pp.37-43
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    • 2020
  • This study was conducted to evaluate the effects of different nitrogen levels on the yield and nutrient quality of a newly developed domestic hybrid of Teosinte, Geukdong 6〔Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis〕. The field experiment was conducted in a randomized block design with three replicates and consisted of four nitrogen (N) application rates, T1 (200 kg/ha), T2 (300 kg/ha), T3 (400 kg/ha), and T4 (500 kg/ha). No differences were found in plant length, leaf length, leaf width, leaf number, dead leaves, stem hardness, tiller number, and fresh yield (p>0.05). The T3 showed significantly greater dry matter yield at harvest (heading stage) compared to the other treatments (p<0.05). The crude protein content of T4 (10.49%) was higher than those of T1 and T2 (p<0.05). However, there was no significant difference between T4 (10.49%) and T3 (9.63%). The effects on crude fat, crude ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude fiber were not significant (p>0.05). The sugar content was higher in the T2 treatment than the other treatments (p<0.05). For Ca, T3 showed significantly greater content (p<0.05). However, no significant effects were found in the contents of Cu, Fe, K, Mg, Mn, Mo, and Zn (p>0.05). Na content was higher in order of T2 > T4 > T3 > T1 (p<0.05). Total mineral contents were not significantly different among the treatments (p>0.05). Given these results, we recommend the amount of nitrogen fertilization necessary for "Geukdong 6" to be around 400 kg per ha (T3), when considering, high fresh yield, dry matter yield, number of leaves and content of crude protein.

Effect of pinewood nematode on the water content and early disease development of seedlings of susceptible Pinus densiflora and resistant Pinus × rigitaeda for breeding for resistance to pinewood nematode

  • Woo, Kwan-Soo;Yoon, Jun-Hyuck;Fins, Lauren;Lee, Do-Hyung;Koo, Yeong-Bon;Yeo, Jin-Kie
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.377-384
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    • 2009
  • Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. ${\times}$ rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. ${\times}$ rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. ${\times}$ rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. ${\times}$ rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. ${\times}$ rigitaeda.

Tetramethylpyrazine Protects Oxidative Stability and Gelation Property of Rabbit Myofibrillar Proteins

  • Wang, Jianping;Liu, Ning;Zhang, Feike
    • Food Science of Animal Resources
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    • v.39 no.4
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    • pp.623-631
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    • 2019
  • Tetramethylpyrazine (TMP), an alkaloid rich in Ligusticum wallichii and fermented products, possesses multiple pharmacological activities in antioxidant, antiinflammatory, and antibacterial. This study aimed to investigate the effect of TMP (15 mg/L) on the physicochemical and gelation properties of rabbit myofibrillar proteins (MPs) with/without oxidative stress. Results showed that compared to the control, oxidative stress to MPs decreased free thiol content, gel yield, whiteness, water-holding capacity, bounder water, immobilized water, and endogenous tryptophan fluorescence intensity, but increased surface hydrophobicity, dityrosine content, and free water content (p<0.01). Without oxidative stress, MPs treated with TMP increased free thiol content, whiteness, and bound water, but decreased dityrosine content and free water (p<0.05). Under oxidative conditions, all parameters were conversely affected by TMP (p<0.01). The results suggest that TMP can be an antioxidant to decrease the concern on oxidative deterioration during meat processing and storage by improving the oxidative stability, water retention, and gel forming property of rabbit MPs.

Pork Quality Traits According to Postmortem pH and Temperature in Berkshire

  • Kim, Tae Wan;Kim, Chul Wook;Yang, Mi Ra;No, Gun Ryoung;Kim, Sam Woong;Kim, Il-Suk
    • Food Science of Animal Resources
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    • v.36 no.1
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    • pp.29-36
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    • 2016
  • This study was performed to investigate the role of pH and temperature postmortem, and to demonstrate the importance of these factors in determining meat quality. Postmortem pH45min (pH at 45 min postmortem or initial pH) via analysis of Pearson’s correlation showed high positive correlation with pH change pHc24 (pH change from pH45min to pH24h postmortem). However, postmortem pH after 24 h (pH24h or ultimate pH) had a high negative correlation with pH change, pHc24, CIE L*, and protein content. Initial temperature postmortem (T1h ) was positively associated with a change in temperature from 45 min to 24 h postmortem (Tc24) and cooking loss, but negatively correlated with water holding capacity. Temperature at 24 h postmortem (T24h) was negatively associated with Tc24. Collectively, these results indicate that higher initial pH was associated with higher pHc24, T1h, and Tc24. However, higher initial pH was associated with a reduction in carcass weight, backfat thickness, CIE a* and b*, water holding capacity, collagen and fat content, drip loss, and cooking loss as well as decreased shear force. In contrast, CIE a* and b*, drip loss, cooking loss, and shear force in higher ultimate pH was showed by a similar pattern to higher initial pH, whereas pHc24, carcass weight, backfat thickness, water holding capacity, fat content, moisture content, protein content, T1h, T24h, and Tc24 were exhibited by completely differential patterns (p<0.05). Therefore, we suggest that initial pH, ultimate pH, and temperatures postmortem are important factors in determining the meat quality of pork.

Extended-CAN Mechanism to Support Keyword Search (키워드 검색 지원을 위한 확장 CAN 메커니즘)

  • Lee, Myoung-Hoon;Park, Jung-Soo;Cho, In-June
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.3
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    • pp.421-429
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    • 2006
  • Recently, DHT-based Structured P2P System have a attention to scalability and providing efficient lookup by routing. Retrieving content of DHT-based P2P require knowledge of the exact identifier of sharing file. But user may wish to search for sharing file using descriptive keyword or content. To resolve the problem, this paper propose Extended-CAN mechanism to support keyword search. We defined content-based keyword and identifier of sharing file, and PLS extended to KID and CKD for keyword, common keyword processing. As a result, Extended-CAN mechanism provide efficient keyword search for DHT-based Structured P2P System.