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Inhibitory Effects of Ethaverine on Serotonin Content in Murine Mastocytoma P815 Cells  

Kim, Eung-Il (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Shin, Jung-Soo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Lim, Sung-Cil (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Park, Seung-Kook (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
YAKHAK HOEJI / v.51, no.2, 2007 , pp. 83-87 More about this Journal
Abstract
The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5${\sim}$10 ${\mu}$M decreased serotonin content in a concentration-dependent manner. The IC$_{50}$ value of ethaverine was 6.1 ${\mu}$M. Ethaverine at concentrations up to 20 ${\mu}$M was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5 ${\mu}$M), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.
Keywords
ethaverine; serotonin content; tryptophan hydroxylase; P815 cells;
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