• 에너지공학
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• 제23권2호
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• pp.62-73
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• 2014

본 연구는 혐기성소화조에서 발생된 바이오가스로부터 바이오메탄을 생산하기 위한 고질화 공정의 운전조건을 최적화하기 위하여 반응표면 분석모델을 적용하였다. 반응표면 분석법의 하나인 Box-Behnken 설계법을 이용하였으며 바이오가스 고질화 공정의 메탄농도와 메탄회수율을 극대화하기 위한 수학적인 최적운전조건을 도출하였다. 도출된 반응표면모델의 적합성을 검증한 결과 각 모델의 p Value가 0.05 이하로서 유의성이 매우 높게 나타났으며, 결정계수($R^2$)는 각각 0.9788, 0.9710 이었다. 그리고 이산화탄소/메탄분리공정에서 메탄농도에 대해 운전압력이 가장 크게 영향을 미치고 다음으로 바이오메탄 생산량, PSA 회전밸브 속도의 순이다. 메탄회수율에 대해서는 PSA 회전밸브 속도가 가장 크게 영향을 미치고 있으며, 바이오메탄 생산량, 운전압력의 순으로 나타났다. 액체바이오 메탄 생산량이 $100Nm^3/hr$일 때의 최적 운전조건을 도출한 결과, 운전압력이 8.0bar 그리고 PSA 회전 밸브 속도가 31.55RPM일 때 바이오메탄의 메탄농도와 메탄회수율을 최대화할 수 있었고, 이때의 바이오메탄의 메탄농도는 97.13%이고, 메탄회수율은 75.89%이었다.

• 한국초등수학교육학회지
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• 제15권3호
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• pp.559-577
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• 2011

본 연구는 수학교실에서 학생들에게 능동적으로 학습에 참여할 기회를 제공하고 의사소통에 있어서 자신의 아이디어를 표현하는 가장 기본적인 전달 방법인 말하기 경험을 확대하는 담화 중심 수학 수업을 초등학교 5학년 학생들에게 적용해 봄으로써 담화 중심 수학 수업이 학생들의 수학적 태도와 수학 학업성취도에 어떤 영향을 미치는지 알아보기 위한 것이다. 그 결과로 담화 중심 수학 수업은 수학 학업성취도의 향상에는 유의미한 차이가 나타나지 않았으나 수학적 태도 중 융통성, 의지력, 호기심, 반성, 가치에서 통계적으로 유의미한 차이가 있는 것으로 나타났으며 수학적 태도에 매우 긍정적인 영향을 주었다. 또한 담화 중심 수업에 대한 설문 조사 및 서술형 평가 등을 통해 담화 중심 수학 수업이 학생들에게 문제 해결에 있어 다양한 방법을 모색해보는 기회를 제공하였으며 흥미와 호기심을 갖고 수업에 참여하도록 할뿐만 아니라 문제를 단순하게 푸는 차원을 넘어서서 원리를 발견하는 경험을 하고 있음을 알 수 있었다. 이렇게 볼 때 담화 중심 수학 수업은 수학적 태도에 긍정적인 영향을 주며 의사소통 능력 신장에도 도움을 줄 수 있다는 결론을 얻을 수 있다.

• 한국환경복원기술학회지
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• 제7권2호
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• pp.75-86
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• 2004

This study aims to figure out ecological characteristic of natural forests focusing on vegetation as a way of ecological renovation for the restoration of naturalness for golf courses that were constructed in the Country and to present vegetation models and appropriate tree species for the purpose. The study site is P golf club, which is located in Gapyong-gun, Kyeonggi Province. The site is within a forest where the grade from the natural ecology map is the first one and the level from the green index accounts for the eight, thus showing a typical environment for a golf course in terms of location. The location of the site explains a reason for restoration. The major substance of the study is to conduct ecological evaluation of vegetation structures around and inside the golf course and to present a vegetation model. In order to evaluate the ecological characteristics of the vegetation structures, the analysis of the study covered succession stages, multi-layer vegetation structures and species diversity. Plant communities that have high species diversity were selected for the vegetation model and proper density and species were proposed considering the number of species and individuals and distances between trees. The vegetation restoration model targets succession into an oak forest. Within a unit of 100 $m^2$, the recommended model include a tall-tree layer with 11 trees such as Quercus serrata and Quercus mongolica, a sub-tall-tree layer with 12 trees including Quercus mongolica, Quercus serrata, Prunus sargentii, Fraxinus rhynchophylla and Acer pseudo sieboldianum, a shrub layer with 32 trees from 16 species, and a grass layer with a cover rate of 45 %. The proposed vegetation restoration model needs to apply to : 1) damaged natural forests by the construction of golf courses; 2) boundaries between golf courses and surrounding forests; 3) buffer zones; 4) open spaces in between courses; and 5) areas between greens and tees where open spaces are available in a mass. In conclusion, one of the most important factors in presenting a vegetation model for the restoration of naturalness in the golf course and other damaged forests is to provide multi-layer vegetation structures that are composed of native species. As for the specific application for the site, it is recommended to manage the vegetation in such a way that the environment of the site can have a similar environment to the surrounding forest which is expected to succeed into an oaks-dominant one.

• 감성과학
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• 제15권1호
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• pp.87-96
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• 2012

본 논문에서는 사용자의 감성적 요구에 적합한 이미지를 탐색한 후 문-스펜서(P. Moon&D. E. Spencer)의 색채 조화론에 기초하여 조화의 정도가 가장 높은 이미지들을 순위별로 제시하는 이미지 검색법을 제안하였다. 이미지 검색은 키워드로 주어진 감성어휘와 관련된 색채성분이 가장 많이 포함된 이미지 화상을 검색하여 미도(Aesthetic Measure)를 계산한 후 순위별로 제시하는 방법으로 이루어진다. 문-스펜서의 색채 조화론을 적용한 이미지 검색법의 타당성을 검증하기 위하여 200개의 샘플 이미지를 대상으로 시스템이 제시한 미도순위와 사용자 만족도 평가에 의한 순위를 비교하였다. 분석결과 15개의 감성어휘별로 시스템이 출력한 이미지들의 미도 순위에 대한 실험 참가자들의 평균 만족도는 7점 척도 중 5.0으로 나타났다. 또한 시스템이 산출한 이미지들의 미도 순위와 설문 참가자가 평가한 만족도 순위 사이와의 일치 여부를 알아보기 위해 상관분석을 실시한 결과 감성어휘 'Clear'를 제외한 14개의 어휘 모두에서 상관계수 0.5 이상의 양호한 정적상관을 보였다. 이와 같은 연구결과로부터 문-스펜서의 색채 조화론을 바탕으로 제안한 감성검색법이 이미지 데이터베이스와 같은 시각자극의 검색에 있어서도 사용자의 감성을 적절히 반영할 수 있는 가능성을 확인하였다.

• 한국수정란이식학회지
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• 제31권1호
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• pp.1-7
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• 2016

Xenotransplantation involves multiple steps of immune rejection. The present study was designed to produce nuclear transfer embryos, prior to the production of transgenic pigs, using fibroblasts carrying transgenes human complement regulatory protein hCD59 and interleukin-18 binding protein (hIL-18BP) to reduce hyperacute rejection (HAR) and cellular rejection in pig-to-human xenotransplantation. In addition to the hCD59-mediated reduction of HAR, hIL-18BP may prevent cellular rejection by inhibiting the activation of natural killer cells, activated T-cell proliferation, and induction of $IFN-{\gamma}$. Transgene construct including hCD59 and ILI-18BP was introduced into miniature pig fetal fibroblasts. After antibiotic selection of double transgenic fibroblasts, integration of the transgene was screened by PCR, and the transgene expression was confirmed by RT-PCR. Treatment of human serum did not affect the survival of double-transgenic fibroblasts, whereas the treatment significantly reduced the survival of non-transgenic fibroblasts (p<0.01), suggesting alleviation of HAR. Among 337 reconstituted oocytes produced by nuclear transfer using the double transgenic fibroblasts, 28 (15.3%) developed to the blastocyst stage. Analysis of individual embryos indicated that 53.6% (15/28) of embryos contained the transgene. The result of the present study demonstrates the resistance of hCD59 and IL-18BP double-transgenic fibroblasts against HAR, and the usefulness of the transgenic approach may be predicted by RT-PCR and cytolytic assessment prior to actual production of transgenic pigs. Further study on the transfer of these embryos to surrogates may produce transgenic clone miniature pigs expressing hCD59 and hIL-18BP for xenotransplantation.

• 한국수정란이식학회지
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• 제30권3호
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• pp.121-128
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• 2015

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed ($37^{\circ}C$) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at $38.5^{\circ}C$ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

• 대한지역사회영양학회지
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• 제19권5호
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• pp.490-498
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• 2014

Objectives: This study was conducted to propose the need of re-establishing the criteria of the body weight classification in the elderly. We compared the Asia-Pacific Region Criteria (APR-C) with Entropy Model Criteria (ENT-C) using Morbidity rate of chronic diseases which correlates significantly with Body Mass Index (BMI). Methods: Subjects were 886 elderly female participating in the 2007-2009 Korea National Health and Nutrition Examination Survey (KNHANES). We compared APR-C with those of ENT-C using Receiver Operating Characteristics (ROC) curve and logistic regression analysis. Results: In the case of the morbidity of hypertension, the results were as follows: Where it was in the T-off point of APR-C, sensitivity was 67.5%, specificity was 43.1%, and Youden's index was 10.6. While in the cut-off point of ENT-C, it was 56.7%, 56.6%, and 13.3 respectively. In the case of the morbidity of diabetes, the results were as follows: In the cut-off point of APR-C, Youden's index was 14.2. While in the cut-off point of ENT-C, it was 17.2 respectively. The Area Under the ROC Curve (AUC) of the subjects who had more than 2 diseases among hypertension, diabetes, and dyslipidemia was 0.615 (95% CI: 0.578-0.652). Compared to the normal group, the odds ratio of the hypertension group which will belong to the overweight or obesity was 1.79 (95% CI: 1.30-2.47) in the APR-C, and 2.04 (95% CI: 1.49-2.80) in the ENT-C (p < 0.001). Conclusions: We conclude that the optimal cut-off point of BMI to distinguish between normal weight and overweight was $24kg/m^2$ (ENT-C) rather than $23kg/m^2$ (APR-C).

• 한국의류학회지
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• 제34권6호
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• pp.955-968
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• 2010

Three-dimensional body data has been used in many industry fields including the apparel industry. This research used data from a study of the changes in lower body girth measurements from a 3D scan study of 25 female subjects aged 18 to 24 in four postures; a standing posture, a $120^{\circ}$ knee bend posture, a one pace stepping posture, and a sitting posture with a $90^{\circ}$ knee bend. We used the information on the difference between standing and seated measurements to adjust ease values for pants patterns an evaluation of the appearance, and the comfort of the pants. Waist girth in the sitting posture increased 8% compared to a standing posture and the hip girth measurement increased 7%. A basic pants pattern (pants A) with 2.4cm ease at the waist and 2.6cm ease at the hip was developed and a pants pattern (pants B) was developed using the rates of lower body girth change with a 5.7cm ease (8% change) at the waist and 7cm ease (7% change) at the hip. The appearance assessment items of pants A in a standing posture were higher than pants B. On the other hand, most appearance assessment items of pants B in a sitting posture were higher than pants A, especially the ease of pants back waistline and the appearance of the whole back. Comfort assessment items of pants B in both standing and sitting postures were higher than the comfort assessment items for pants A, especially the location of pants waistline, the ease of pants at the waistline, and the ease at the abdomen. In order to find the best level of ease for better appearance and comfort in both standing and sitting postures, 20 pants were constructed with ease values at the waist and hip in increments of 1.1cm in the range between the ease values of pants A and pants B. A fit test was conducted to compare the average appearance and comfort ratings that identified the pants with the best ease values at the waist and hip. The highest total mean was achieved in the pants with a waist ease of 4.6cm and hip ease of 4.8cm.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.197-205
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• 2002

Volatile organic compounds (VOCs) are an important public health problem throughout the world. Many important questions remain to be addressed in assessing exposure to these compounds. Because they are ubiquitous and highly volatile, special techniques must be applied in the analytical determination of VOCs. Personal exposure measurements are needed to evaluate the relationship between microenvironmental concentrations and actual exposures. It is also important to investigate exposure frequency, duration, and intensity, as well as personal exposure characteristics. In addition to air monitoring, biological monitoring may contribute significantly to risk assessment by allowing estimation of absorbed doses, rather than just the external exposure concentrations, which are evaluated by environmental and personal monitoring. This study was conducted to establish the analytic procedure of VOCs in air, blood, urine and exhaled breath and to evaluate the relationships among these environmental media. The subjects of this study were selected because they are occupationally exposed to high levels of VOCs. Environmental, personal, blood, urine and exhalation samples were collected. Purge ＆ trap, thermal desorber, gas chromatography and mass selective detector were used to analyze the collected samples. Analytical procedures were validated with the“break through test”, 'quot;recovery test for storage and transportation”,“method detection limit test”and“inter-laboratory QA/QC study”. Assessment of halogenated compounds indicted that they were significantly correlated to each other (p value < 0.01). In a similar manner, aromatic compounds were also correlated, except in urine sample. Linear regression was used to evaluate the relationships between personal exposures and environmental concentrations. These relationships for aromatic and halogenated are as follows: Halogen $s_{personal}$ = 3.875+0.068Halogen $s_{environmet}$, ($R^2$= .930) Aromatic $s_{personal}$ = 34217.757-31.266Aromatic $s_{environmet}$, ($R^2$= .821) Multiple regression was used to evaluate the relationship between exposures and various exposure deter-minants including, gender, duration of employment, and smoking history. The results of the regression model-ins for halogens in blood and aromatics in urine are as follows: Halogen $s_{blood}$ = 8.181+0.246Halogen $s_{personal}$+3.975Gender ($R^2$= .925), Aromatic $s_{urine}$ = 249.565+0.135Aromatic $s_{personal}$ -5.651 D.S ($R^2$ = .735), In conclusion, we have established analytic procedures for VOC measurement in biological and environmental samples and have presented data demonstrating relationships between VOCs levels in biological media and environmental samples. Abbreviation GC/MS, Gas Chromatography/Mass Spectrometer; VOCs, Volatile Organic Compounds; OVM, Organic Vapor Monitor; TO, Toxic Organicsapor Monitor; TO, Toxic Organics.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.265-272
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• 2002

Enzyme-Inhibition방법으로 다성분 잔류 농약의 검출 기법을 개발하기 위해, 음용수 허용기준 설정 농약인 유기인계 농약으로 malathion, parathion, diazinon과 carbamate농약으로 carbary에 대한 acetylcholinesterase (AChE)과 cholinesterase (ChE) 활성저해 관계를 규명하였다. 병아리 뇌의 AChE와 ChE 활성도는 각각 166.6 및 5.8$\mu$mol/min/g protein이었고, 혈장에서는 각각 23.1$\mu$mol/min/g protein과 8.3 $\mu$mol/min/g protein 이었다. AChE와 ChE의 최적 PH는 각각 8.2및 7.8 이었다. Km은 0.034 및 0.045 mM 이었다. 유기인계농약에서 AChE와 ChE의 I$_{50}$ 값의 malathion이 55.82 및 99.42mg/L이었고, Parathion은 31.16및 29.13mg/L이었고, diazinon은 17.89 및 19.62 mg/L 이었다. Carbamate농약인 carbaryl의 AChE와 ChE의 I$_{50}$ 값의 0.10및 0.05mg/L이었다. 먹는 물 관리법에 의한 carbaryl의 먹는 물 허용 수질기준인 0.07mg /L을 AChE와 ChE의 I$_{50}$에서 검출할 수 있다. AChE및 ChE을 이용한 enzyme-inhibition(EI)법은 carbamate농약인 carbaryl을 먹는 물 허용 수질기준인 0.07mg/L가지 검출 할 수 있으므로, 다성분 잔류분석법 (MRM, Multiresidue Method)으로 이용할 수 있다. 따라서 Enzyme lnhibition방법을 이용하여 자연환경 중 carbamate계 농약을 쉽고 빠르게 검출할 수 있는 새로운 bioassay법으로 응용할 수 있다. 수 있다.