• Journal of the Korean Chemical Society
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• v.38 no.8
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• pp.608-615
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• 1994

The isolation, purification and characterization of a carotenoprotein from the carapace of Pnaeus orientalis were investigated. The carotenoprotein was purple with broad λmax between 480, 409, 318 and 280 nm. Apparent structures were estimated by using X-ray diffractometry and scanning electron microscope, respectively. The molecular weight of the carotenoprotein complex had been determined by GPC and PAGE. The heavier complex, designated the $\alpha$-form (M.W = 170 KDa), was dissociated to a major subunit, $\beta$-form (M.W = 42 KDa). SDS-PAGE of $\alpha$-form showed apparently oligomeric pattern, and also $\beta$-form gave two polypeptides corresponding to 22 KDa and 19 KDa, respectively. The amino acid of the two proteins $({\alpha}-and\;{\beta})$-form, lipid and free fatty acid compositions were described. The prosthetic groups of the carotenoprotein were confirmed by TLC, IR, $^1H$-NMR, MS and various organic reactions as astaxanthin, astaxanthin monoester and astaxnathin diester.

• Archives of Pharmacal Research
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• v.22 no.4
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• pp.404-409
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• 1999

The inhibitory effects of the constituents of Gastrodia elata Bl. (GE) on glutamate-induced apoptosis in human neuronal cells were investigated using IMR32 human neuroblastoma cells. Glutamate (GLU) induced DNA fragmentation, a hallmark of apoptosis, in a dose-dependent manner. GLU also induced a slow and sustained increase in intracellular $Ca^{2+}$ concentration. Treatment with EGTA, an extracellular $Ca^{2+}$ chelator, in a nominal $Ca^{2+}$ -free buffer solution abolished the GLU-induced intracellular $Ca^{2+}$ increase, indicating that GLU stimulated Ca2+ influx pathway in the IMR32 cells. BAPTA, an intracellualr $Ca^{2+}$ chelator, significantly inhibited the GLU-induced apoptosis assessed by the flow cytometry measuring hypodiploid DNA content indicative of apoptosis, implying that intracellular $Ca^{2+}$ rise may mediate the apoptotic action of GLU. Vanillin (VAN) and p-hydroxybenzaldehyde(p-HB), known constituents of GE, significantly inhibited both intracellular $Ca^{2+}$ rise and apoptosis induced by GLU. These results suggest that the apoptosis-inhibitory actions of the constituents of GE may account, at least in part, for the basis of their antiepileptic activities. These results further suggest that intracelluarl $Ca^{2+}$ signaling pathway may be a molecular target of the constituents of GE.

• Journal of Society of Preventive Korean Medicine
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• v.13 no.2
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• pp.65-76
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• 2009

This study was to examine of physical and psychological traits to sasang constitution in college students. The subjects in this study were 114 people, who were consists of 35 persons of Taeeumin, 34 persons of Soyangin and 45 persons of Soeumin. We were analyzed psychological trait with the Myers-Briggs Type Indicators(MBTI) and State-Trait Anxiety Inventory(STAI) according to sasang constitution. Also, We were analyzed physical trait with the Body Mass Index(BMI) according to sasang constitution. Each sasang constitutions personality type showed significantly differences only in the extraversion and introversion(p=0.012). In the relationship of BMI, Taeeumin showed bigger BMI compared to others (p<0.001). As a result of logistic regression analysis, Soyangin was higher in the extraversion(odds ratio[OR], 3.48 ; 95% confidence interval [95% CI], 1.18-10.26) compared with Taeeumin. Also, each sasang constitutions showed significantly differences in trait-anxiety(p=0.047). The score of trait-anxiety in Soeumin was significantly higher than that in Taeeumin. The limitation of this study was that particular samples could not represent the population, because the students from one college had participated in this study. In the future, we will perform this study in general population. At the same time, we will also develop approaches considering physical and psychological aspects depending on the types of constitution in the management of subjects.

• Archives of Plastic Surgery
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• v.45 no.3
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• pp.239-245
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• 2018

Background Breast reconstruction has become more common as mastectomy has become more frequent. In Korea, the National Health Insurance Service (NHIS) began covering breast reconstruction in April 2015. This study aimed to investigate trends in mastectomy and breast reconstruction over the past 10 years and to evaluate the impact of NHIS coverage on breast reconstruction. Methods Nationwide data regarding mastectomy and breast reconstruction were collected from the Korean Breast Cancer Society registry database. Multiple variables were analyzed in the records of patients who underwent breast reconstruction from January 2005 to March 2017 at a single institution. Results At Seoul National University Hospital, the total number of reconstruction cases increased 13-fold from 2005 to 2016. The proportion of immediate breast reconstruction (IBR) cases out of all cases of total mastectomy increased from 4% in 2005 to 52.0% in 2016. The proportion of delayed breast reconstruction (DBR) cases out of all cases of breast reconstruction and the overall number of DBR cases increased from 8.8% (20 cases) in 2012 to 18.3% (76 cases) in 2016. After NHIS coverage was initiated, the proportions of IBR and DBR showed statistically significant increases (P<0.05). Among the IBR cases, the percentage of prosthesis-based reconstructions increased significantly (P<0.05), but this trend was not found with DBR. Total mastectomy became significantly more common after the expansion of NHIS coverage (P<0.05). Conclusions Over the last decade, there has been an increase in mastectomy and breast reconstruction, and the pace of increase accelerated after the expansion of NHIS coverage. It is expected that breast reconstruction will be a routine option for patients with breast cancer under the NHIS.

• Archives of Plastic Surgery
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• v.48 no.1
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• pp.33-43
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• 2021

Background Acellular dermal matrices (ADMs) have become an essential material for implant-based breast reconstruction. No previous studies have evaluated the effects of sterility of ADM under conditions of radiation. This study compared sterile (irradiated) and aseptic (non-irradiated) ADMs to determine which would better endure radiotherapy. Methods Eighteen male Balb/C mice were assigned to the control group with no irradiation (group 1) or one of two other groups with a radiation intensity of 10 Gy (group 2) or 20 Gy (group 3). Both sterile and aseptic ADMs were inserted into the back of each mouse. The residual volume of the ADM (measured using three-dimensional photography), cell incorporation, α-smooth muscle actin expression, and connective tissue growth factor expression were evaluated. The thickness and CD3 expression of the skin were measured 4 and 8 weeks after radiation. Results In groups 2 and 3, irradiated ADMs had a significantly larger residual volume than the non-irradiated ADMs after 8 weeks (P<0.05). No significant differences were found in cell incorporation and the amount of fibrosis between irradiated and non-irradiated ADMs. The skin was significantly thicker in the non-irradiated ADMs than in the irradiated ADMs in group 3 (P<0.05). CD3 staining showed significantly fewer inflammatory cells in the skin of irradiated ADMs than in non-irradiated ADMs in all three groups after 4 and 8 weeks (P<0.05). Conclusions Under radiation exposure, irradiated ADMs were more durable, with less volume decrease and less deposition of collagen fibers and inflammatory reactions in the skin than in non-irradiated ADMs.

• Journal of Environmental Science International
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• v.32 no.1
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• pp.67-76
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• 2023

Under constant environmental pollution, the incidence of Atopic Dermatitis (AD) caused by air pollutants and allergens has increased. AD is an allergy inflammatory skin disease characterized by pruritus, eczema, and skin dryness. In herbal medicine, Anemarrhena asphodeloides (Anemarrhenae Rhizoma; AR) has been utilized to treat Alzheimer's disease, osteoporosis, hypertension, and inflammation. The purpose of study evaluated the effect of AR in a mouse model of 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions. After acclimatization for 5 days, the mice (6-week-old, male Balb/c) were divided into five groups (n=6/group): NC (normal control), DNCB (control), Dex (5 mg·kg-1, p.o.), AR100 (100 mg·kg-1, p.o.), and AR300 (300 mg·kg-1, p.o.). On days 1 and 3, 1% DNCB was applied to the skin and ears. After 4 days, 0.5% DNCB was applied once every 2 days for 2 weeks. Then, skin and ears eczema area and severity index (EASI); skin nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) levels; and plasma immunoglobulin E (IgE) levels were examined. The AR groups showed lower EASI, skin and ear thickness, mast cell count, and IgE levels than the control groups. Moreover, AR reduced iNOS, COX-2, and PGE2 levels. Therefore, AR possesses anti-inflammatory properties and can improve skin damage, indicating its therapeutic potential against AD.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.3
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• pp.304-311
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• 1985

This study was carried out to find the spring-season weed growth status on account of different soil physicochemical properties due to continuous application of same fertilizer for 8 years at paddy field. The results examined on weed species, dry weight of weed, and weed growth status were summarized as follows. 1. 1-6 species of weed occurred in the plots treated by continueous application of different fertilizers. Cyperus serotinus could grow only in the phosphate defficient plots, however, in the N+P+K, N+P and N+P+K+compost plots, 4-6 kinds of weed occurred variously. 2. In the plots applied phosphate like as, P, P+K, N+P+K+compost, and N+P+K+plots, large amount of weed occurred apparently. 3. Positive correlation betwaen dry weight of Alopeculus aequalis, polygonum hydropiper and Chenopodium alburn and available phosphate content in soil was showed with high significance. 4. Available phosphate in soil affected weed growth and weed species composition among weed communities. Application of lime fertilizer tended to suppress the occurrence of Alopeculus aequalis. 5. Dry matter of polygonum Longisetum, Chenopodium album and polygonum hydropiper showed higher content of nitrogen, phosphate, calcium, and magnesium. Whereas those of Alopeculus aequalis, Cyperus Serotinus and Stellaria alsine were less.

• Journal of the Korean Geotechnical Society
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• v.23 no.2
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• pp.71-83
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• 2007

Liquefaction resistance depends strongly upon the degree of saturation, which is expressed in terms of the pore pressure coefficient, B. The B-value has been widely used to quantify the state of saturation of laboratory samples. However, it is practically impossible to determine in situ state of saturation by using the B-value. So, P-wave velocity can be alternatively used as a convenient index for evaluating the in situ state of saturation. In this paper, the Stokoe type torsional shear (TS) testing system was modified to saturate the specimen, with which it is also possible to measure P ($V_p$), S-wave velocity ($V_s$) and the excess pore water pressure buildup In order to examine the effect of B-value for nearly saturated sands. A series of the tests were carried out at 3 relative densities (40%, 50% and 75%) and various B-values using Toyoura sand. Based on the test results, the variations of $V_p\;and\;V_s$ with B-value were analyzed and compared with a existing theoretically derived formula. The normalized pore water pressure, $du/{\sigma}{_0}'$ and cyclic threshold shear strain, ${\gamma}^c_{th}$ with B-value were also analyzed. Additionally the test results related to pore water pressure were analyzed by $V_p$ to apply to the field seismic analysis.

• Journal of the Korean Chemical Society
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• v.45 no.1
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• pp.14-24
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• 2001

In order to study the long-term change of water quality, water analysis for 9 items was conducted at 13 sites surrounding the Kumho river system for 18 times from September 1993 to August 1999. Analytical items for the study of water quality are as follows; water temperature, pH, BOD, COD, DO, SS, electrical conductivity, oil and grease, ABS and phenol. The six year term was divided into Part I(second study) and Part Ⅱ(third study), which covers the term from September 1993 to August 1996 and the term from September 1996 to August 1999, respectively. The mean values obtained for three years for water temperature, pH, BOD, COD, DO, SS, electrical conductivity, oil and grease, ABS and phenol for the Part I period showed 18.4$^{\circ}C$, 7.9, 4.88 ppm, 9.66 ppm, 9.0 ppm, 618.0 umho/cm, 6.9 ppm, 2.63 ppm, 3.57 ppm and 0.98 ppm, respectively. The mean values obtained for water temperature, pH, BOD, COD, DO, SS, electrical conductivity, oil and grease, ABS and phenol for the Part Ⅱ period showed 18.0$^{\circ}C$, 8.0, 2.86 ppm, 7.40 ppm, 8.94 ppm, 541.0 umho/cm, 6.0 ppm, 5.43 ppm, 0.94 ppm and 0.01 ppm respectively. The values of BOD, COD, SS, electrical conductivity, ABS and phenol in the second period were found to be decreased by 1/1.71, 1/1.31, 1/4.15, 1/3.80 and 1/98, respectively. The values of water temperature, pH and DO were nearly constant. However, the concentrations of oil and grease were found to be increased about 2.06 times in the second period.

• Korean Chemical Engineering Research
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• v.43 no.5
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• pp.595-602
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• 2005

In order to purify diethylene glycol as main impurity included in p-dioxanone, SLE (solid-liquid equilibria) and mixture density on two components system of p-dioxanone and diethylene glycol were measured and a layered melt crystallization with seed has been applied. The SLE of p-dioxanone and diethylene glycol were a simple eutectic system and the temperature and PDX concentration at eutectic point were 0.08 and 246 K, respectively. Densities of their binary mixtures were well fitted by the best correlation equation, ${\rho}_l=0.405+1.361x+0.002T-0.004xT$. In the melt crystallization, the growth rate (G) was proportional to the 1.5th power of the subcooling degree. The effective distribution coefficient ($K_{eff}$) as the degree of impurity removal was observed to increase with increasing the growth rate and initial p-dioxanone concentration. And also, $K_{eff}$ was correlated with Z function using Wintermantel's model such as $K_{eef}=-0.0604+6.392{\times}Z$. Finally, PDX purity through the optimization of this process can be obtained over 99%.