• Molecules and Cells
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• 제21권2호
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• pp.197-205
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• 2006

We used cDNA microarrays to monitor the transcriptome of ozone stress-regulated genes (ORGs) in two pepper cultivars [Capsicum annuum cv. Dabotop (ozone-sensitive) and Capsicum annuum cv. Buchon (ozone-tolerant)]. Ozone stress up- or down-regulated 180 genes more than three-fold. Transcripts of 84 of these ORGs increased, transcripts of 88 others diminished, and those of eight either accumulated or diminished at different time points in the two cultivars or changed in only one of the cultivars. 67% (120) of the ORGs were regulated differently in ozone-sensitive and ozone-tolerant peppers, most being specifically up-regulated in the ozone-sensitive cultivar. Many were also represented in the plant defense transcriptome against non-host pathogen infection, and some in the transcriptomes for cold, drought, and salinity stresses.

• Forest Science and Technology
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• 제14권4호
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• pp.153-159
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• 2018

Seedlings of Pterocarpus indicus were grown in both well-watered and drought stress conditions in phytotron. Seedlings grown under well-watered and drought stress conditions were exposed to either combined or without ozone of 200 ppb for one month. First, the physiological responses to elevated ozone levels indicated a decreased biomass. The seedlings grown in arid soil and exposed to ozone showed less biomass than those grown in arid soil but not exposed to ozone. Moreover, all the seedlings except the well-watered and unexposed ones showed a significantly lower photosynthetic rate ($P_N$) over time. However, with the accumulation of ozone injuries, the antioxidant enzyme activities increased overall. In the study results, when exposed to ozone, the well-watered seedlings exhibited more antioxidative enzyme activity than did the seedlings grown in arid soil. Generally, P. indicus in arid soil suffered less damage from elevated ozone than did the well-watered plants.

• 한국작물학회지
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• 제46권2호
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• pp.78-83
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• 2001

Two rice cultivars of the japonica type, ozone-resistant Ilpumbyeo (IL) and ozone-susceptible Keu-mobyeo#l (KM) were exposed to ozone ($O_3$) at 0.15 ppm for 30 days. The available nutrient regimes were varied by doubling the supply of nitrogen (N), phosphorus (P) and potassium (K) within a basic fertilizer status (N, P, K; 15, 12, 12 kg l0$a^{-1}$). There was little difference on plant height between ozone-treated and nontreated plants. The most significant ozone stress on tiller number was shown on the 30th day of ozone exposure. Slight recovery from ozone stress was noted on the 60th day. On the 30th day, tiller number was greatly decreased by 40.8% in IL and 64.6% in KM, whereas at a high nitrogen supply regime (2N), it was decreased by 21.4% in IL and 42.7% in KM as compared to the control not treated with ozone at basic fertilizer status. The inhibition of tiller production caused by ozone exposure was alleviated on the 60th day. In both cultivars, number of spikelets per plant and weight of 100 grains were affected little by the ozone treatment irrespective of nutrient regime. However, the number of panicles per plant and yield were reduced significantly. In both cultivars, yield of ozone-treated plants with 2N status was 12.4-16.1 % higher than that of the ozone-treated plants with basic nutrient status. A significant yield decrease of 47.8% and 33.4% was observed for IL and KM, respectively, in ozone-treated plants with higher potassium (2K) status.

• 한국산림과학회지
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• 제86권1호
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• pp.80-86
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• 1997

잡종(雜種)포플러가 어떻게 오존 stress에서 피해(被害)를 최소화하는가를 알아보기 위해서 생장(生長), 광합성율(光合成率), 그리고 초기(初期) Rubisco의 활성(活性)을 조사(調査)하였다. 이 연구(硏究)에서는 Populus trichocarpa${\times}$P. deltoides 삽목묘(揷木苗)를 재료(材料)로 사용(使用)하였다. 오존처리농도(處理濃度)는 90-115 ppb으로, 하루에 6-9시간씩, 126일 동안 처리하였다. 이 연구에서는, 오존에 저항성(抵抗性)을 가지는 포플러는 광합성율(光合成率)과 Rubisco의 활성(活性)을 증가(增加)시켜서 그 물질(物質) 생산량을 유지(維持)하여 stress를 보상(補償)한다는 가설(假說)을 검정(檢定)하였다. 생장(生長), 물질생산량(物質生産量), 광합성율(光合成率), 그리고 초기(初期) Rubisco 활성(活性)이 일반적으로 오존에 의해서 감소(減少)하였다. 뿌리의 생장량은 지상부(地上部)의 피해(被害)를 보상하기 위한 탄소(炭素)의 불균등(不均等)한 분배(分配)때문에, 가장 피해가 심한 부위(部位)였다. 저항성을 가지는 포플러는 오존에 대해서 수체내(樹體內)의 광합성율(光合成率)과 초기 Rubisco 활성을 높여 생산량(生産量)을 유지(維持)하는 생물적(生物的)인 보상(補償)을 하는 것으로 밝혀졌다.

• The Korean Journal of Ecology
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• 제20권2호
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• pp.83-94
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• 1997

Stomatal closing by ozone and water stress could reduce further ozone injury by inhibition of ozone influx to the tissue. Direct effect of ozone on stomata can be explained from two aspects which are a stimulation of stomatal closing and an inhibition of stomatal opening. An increase of $Ca^{2+}$ influx into cytoplasm by ozone could stimulate potassium efflux ion channel and inhibits inward potassium ion channels. By this mechanism ozone could induce stomatal closing. On the other hand, ozone could inhibit stomatal opening by affecting the activity of $H^{+}$ dependent ATPase of the membrane in guard cells. This would inhibit proton efflux which precede stomatal opening. It is also possible that ozone could reduce the activity of photosynthesis in guard cells which lead to affect the production of osmotically active sugars and energy. Indirect effect of ozone to stomata is through the effect of $CO_2$ elevation as a result of damage of the photozynthetic machinery. This indirect effect is slower than the direct effect.

• 한국산림과학회지
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• 제97권5호
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• pp.508-515
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• 2008

Sycamore (Platanus occidentalis L.) seedlings were grown under low light intensity and ozone treatments to investigate the role of the light environment in their response to chronic ozone stress. One-year-old seedlings of Platanus occidentalis L. were grown in pots for 3 weeks under low light (OL, $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and high light (OH, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) irradiance in combination with 150 ppb of ozone fumigation. After three weeks of ozone and light treatment, seedlings were placed in ozone free clean chamber for 3 weeks for recovery from ozone stress with same light conditions to compare recovery capacity. Ozone fumigation determined an impairment of the photosynthetic process. Reduction of leaf dry weight (14%) and shoo/root ratio (17%) were observed in OH treatment. OL treatment also showed severe reductions in leaf dry weight and shoot/root ratio by 48% and 36% comparing to control, respectively. At the recovery phase, OH-treated plants recovered their biomass, whereas OL-treated plant showed reduction in leaf dry weight (52%) and shoot/root ratio (49%). OH-treated plants reached similar relative growth rate (RGR) comparing to control, whereas OL-treated plants showed lower RGR in stem height. However, there were no significant differences in response to those treatments in stem diameter RGR at the recovery phase. Ozone treatment produced significant reduction of net photosynthesis in both high and low light treatments. Carboxylation efficiency and apparent quantum yield in OL-treated plants showed significant reductions rate to 10% and 45%, respectively. At the recovery stage, ozone exposed seedlings under high light had similar photosynthetic capacity comparing to control plants. Antioxidant enzymes activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) were increased in ozone fumigated plants only under low light. The present work shows that the physiological changes occur in photosynthesis-related parameters and growth due to ozone and low light stress. Thus, low light seems to enhance the detrimental effects of ozone on growth, photosynthesis, and antioxidant enzyme responses.

• 한국산림과학회지
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• 제87권1호
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• pp.50-56
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• 1998

오존에 약(弱)하거나 저항성(抵抗性)을 가지는 포플러 개체(個體)를 선발(選拔)하기 위해서 36개의 잡종(雜種)포플러 클론을 오존에 노출(露出)시켰다. Open-top chamber를 이용(利用)하여 하루에 6-8시간, 3개월 동안 90-115ppb의 농도(濃度)로 오존을 노출시켰다. 수고(樹高), 직경(直徑), 엽수(葉數), 물질생산량(物質生産量), root/shoot비율(比率), 낙엽률(落葉率), 기공반응(氣孔反應) 조사(調査)하였다. 수고, 직경, 엽수, 물질생산량, root/shoot비율, 기공 conductance는 오존 때문에 감소(減少)하였고, 낙엽률은 증가(增加)하였다. 이 연구에서는 기공 conductance와 물질생산량과의 관계(關係)는 아주 낮게 나타났다. 오존환경(環境)에 대해서 저항성을 갖는 것은 기공(氣孔)의 개폐(開閉)와 항상(恒常) 관계(關係)가 있는 것은 아니라고 사료(思料)되며, 아마도 생화학적(生化學的)이고 생리적(生理的)인 다른 요인(要因)에 대(對)한 연구(硏究)가 포플러 클론이 갖는 저항성을 이해(理解)하는데 도움이 될 것이다.

• The Plant Pathology Journal
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• 제16권3호
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• pp.130-136
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• 2000

Differences in physiological and biochemical responses between sensitive and tolerant rice cultivars to ozone were investigated to develop reliable indications of early ozone damage. Three Korean local rice cultivars -sen-sitive cultivar Dongjin (DJ), moderately tolerant cultivar Hwayeong (HY) and tolerant cultivar Ilmee (IM) were exposed to ozone at the concentrations of 100 nl $\textrm{l}^{-1}$ or 200 nl $\textrm{l}^{-1}$ , 8 h per day for 10 days in a controlled-environment fumigation chamber. The rice cultivars seemed to be endurable to ozone stress at the concentration of 100 nl $\textrm{l}^{-1}$ which is frequently monitored during the growing season in summer. However, severe damage was induced and differential sensitivity was clearly noted among the rice cultivars at the higher ozone concentration. Activation of the glutathion (GR) -ascorbate peroxidase (APX) cycle was likely to be responsible for protection of rice plants against ozone exposure, relating difference in sensitivity of rice cultivars to ozone. Photosynthetic activity appeared to be one of sensitive responses, for which chlorophyll fluorescence and leaf greenness can together provide a very reliable index, a degree of photosynthetic damages by ozone. Formation of malondialdehyde (MDA) was also considered as an indication that can differentiate cultivars sensitivity to ozone. However, the changes in polyamines and total phenolics were not consistent with exposed ozone concentrations and/or ozone sensitivity of the cultivars. The behavior of polyamines and phenolics in the damaged plants at high ozone levels could be interpreted as an indication of ozone injury rather than activation of additional protection mechanisms scavenging active oxygen species formed by ozone. Several responses triggered by ozone could explain the differential sensitivity of the rice cultivars and be used as reliable indications of relative ozone damage to rice plant.

• 한국산림과학회지
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• 제97권5호
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• pp.552-554
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• 2008

Acer palmatum was selected and its responses to elevated ozone were subsequently measured during growing periods. Ozone concentration of this study was compared to the calculated AOT40 value. A. palmatum had significantly many but small stomata size to the ozone stress. The length of stomata of A. palmatum was reduced from 5.6 to $5.0{\mu}m$ to the ozone exposure. However, the number of stomata (density) was increased from 102 to 131 in the $500{\times}500{\mu}m$ leaf area.

• 한국작물학회지
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• 제42권3호
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• pp.338-343
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• 1997

벼 품종의 오존 저항성 판단을 위한 시험에서 오존의 처리농도는 0.3 ppm이 0.1 ppm보다 뚜렷한 결과를 보였고, 처리시간은 2∼4시간이 알맞은 것으로 나타났다. 묘대일수에 따른 저항성 정도는 파종후 3주까지는 생육이 진전될수록 높아지는 경향이었으나 3∼5주간에는 큰 차이가 없었다. 인디카와 통일형 품종들이 자포니카 품종보다 저항성이 높은 경향이었고, 자포니카 품종 중에서 장안벼외 7 품종은 높은 저항성을 나타내었다. 벼의 생육시기에 따른 피해엽율은 간척벼는 유묘기에서 밀양 2003외 4 품종은 최고 분열기에서 가장 높게 나타났고 임실율은 수잉기 처리에서 가장 낮았다.