• Journal of Community Nutrition
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• 제7권4호
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• pp.215-219
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• 2005

Oxygen radical absorbance capacity(ORAC) is known to be a sensitive and simple method to determine total antioxidant capacity(TAC) in biological samples. While TAC has received great attention with its relation to pathogenesis in the progression of several diseases, little is known about association of ORAC with metabolic risk factors. The aim of this study is to evaluate the relationship between ORAC and serum lipid profiles, fasting glucose and anthropometric measures. One hundred seventeen volunteers participated in the study. Perchloric acid treated serum was used to determine $ORAC_{pca}$. Mean$ORAC_{pca}$ of subjects whose serum total cholesterol(TC) concentrations were $\geq$ 200 mg/dl was significantly(P < 0.05) higher than that of subjects whose TC concentrations were < 200mg/dl. There were significantly positive correlations between $ORAC_{pca}$ and serum concentrations of TC(P < 0.05) and low density lipoprotein (LDL) cholesterol(P < 0.01). The positive relation between cholesterol concentrations and $ORAC_{pca}$ in serum may suggest an elevated TAC against oxidative stress associated with the cardiovascular risk factors. (J Community Nutrition 7(4): $215\∼219$, 2005)

• Nutritional Sciences
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• 제9권1호
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• pp.48-54
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• 2006

Methods have been developed to evaluate the total antioxidant capacity of foods and plasma but limitations are associated with their ability to determine precisely the contribution of lipophilic antioxidants in a lipid milieu as well as interactions among them Thus, we modified the Oxygen Radical Absorbance Capacity (ORAC) assay to determine the peroxyradical scavenging ability of both hydrophilic and lipophilic compartments in plasma The hydrophilic ORAC assay was performed in a phosphate buffer system utilizing 2,2'-azobis (2-amidinopropane) dihydrochloride as a peroxyradical generator and fluorescein as the target The lipophilic ORAC assay was carried out in a dimethylsulfoxide :butyronitrile (DMSO/BN, 9:1 v/v) system using 2,2'-azobis (2,4-dimethyl valeronitrile) as a peroxyradical generator and BODIPY C11 581/591 as the target Analyses were conducted in bovine serum supplemented with water - and lipid - soluble antioxidants and in human plasma. Albumin (0.5$\sim$5 g/dL) and uric acid (0.1$\sim$0.5 $\mu$mol/L) increased hydrophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.97 and 0.98, respectively) but had no impact on lipophilic ORAC values. $\alpha$-Tocopherol (15$\sim$200 $\mu$mol/L) increased lipophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.94); neither $\alpha$-tocopherol nor $\beta$-carotene had an impact on hydrophilic ORAC values. However, addition of $\beta$-carotene at physiological concentration (0.23$\sim$1.86 $\mu$mol/L), either alone or in combination with other carotenoids, had no significant impact on lipophilic ORAC values. Thus, while assays of 'total antioxidant capacity' in biological matrices would be a useful research and clinical tool, existing methods are limited by the lack of complete responsiveness to the full range of dietary antioxidants.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1505-1510
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• 2009

Arginyl-fructose (AF) and arginyl-fructosyl-glucose (AFG) were chemically synthesized and purified. Their in vitro and cellular antioxidant activity was investigated using oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity assay, respectively. The peroxyl radical scavenging activity of AF was much higher than that of AFG, which was in good agreement with their reduction capacity to donate electrons or hydrogen atoms. On the other hand, the hydroxyl radical scavenging activity of AF was weaker than that of AFG, which was consistent with their metal chelating activity, suggesting that AFG-$Cu^{2+}$ complex may be less redox-active than AF-$Cu^{2+}$ complex due to 1 glucose molecule attached. The cellular antioxidant activity of AF and AFG appeared to depend on both their permeability into cell membrane and the scavenging activity on peroxyl or hydroxyl radicals. These results indicate that AF and AFG, Maillard reaction products, may have a high potential as a material for the development of nutraceutical food with antioxidant activity.

• Food Science and Biotechnology
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• 제18권1호
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• pp.239-244
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• 2009

In the current study, inhibitory activity of 8 selected Korean edible plants of Lamiaceae family against $\alpha$-glucosidases, prepared from rat small intestine acetone powder was evaluated. Total flavonoids and oxygen radical absorbance capacity (ORAC) were also investigated. Methyl alcohol extracts of Scutellaria indica (SI) had the highest $\alpha$-glucosidase inhibitory activity relevant for potentially managing hyperglycemia, followed by Clinopodium gracile (CG) and Thymus quinquecostatus (TQ). These 3 species also showed significant antioxidant activity in ORAC system. The $\alpha$-glucosidase inhibitory activity of the extracts was compared to selected phenolics. Among the standard phenolics tested quercetin which was major flavonoid in the extracts had the highest $\alpha$-glucosidase inhibitory activity. CG, TQ, and SI which had high quercetin content and ORAC values also exhibited significant sucrase inhibitory activity. Results suggested that selected 3 Korean Lamiaceae species have the potential development of effective dietary strategy for postprandial hyperglycemia and oxidative stress-linked diabetes complications.

• 동아시아식생활학회지
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• 제17권3호
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• pp.393-401
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• 2007

인삼의 여러 생리 활성 가운데 항산화 정도를 알아보기 위하여 백삼(6 년근), 백삼(5 년근), 피부백삼(5 년근), 피부백삼 (4 년근) 의 80% 에탄올 엑기스, 에틸아세테이트 분획, 수포화 부탄올 분획, 물 분획을 얻은 후 LC/Mass 를 사용하여 사포닌 함량을 조사하고 기존의 여러 가지 항산화 작용 측정 방법들의 오류를 없애고 더욱 정확한 결과를 낼 수 있는 대처 방안으로 선정된 ORAC Assay에 의해 항산화 활성을 검토하였다. 인삼 중 사포닌은 ginsenoside Rg 1 과 Rb1 이 주요 성분으로 다량 함유하고 있었으며, Rc, Rb2, Re 등이 뒤를 이었고, 그밖에도 Rd, Rg3, Rh1가 널리 분포하고 있었다. 피부백삼 5 년근의 경우 에탄올 엑기스와 수포화 부탄올 분획에서 다른 인삼 분획에 비해 높은 함유량을 보였으나 실험의 한계상 인삼 재배기간과 인삼 종류별 각각의 분획에 대한 사포닌 함량 비교는 어려웠다. 인삼의 각 분획별 항산화 활성은 80% 에탄올 엑기스, 에틸아세테이트 분획, 수포화 부탄올 분획, 물 분획 모두에서 나타났고 비교적 전체 유기 용매 분획의 값이 비슷하였으며, 수층 분획이 다소 낮은 활성을 보였다. 검체 인삼들의 각 용매추출 분획 상호간의 유의성 비교에서는 모든 인삼 검체의 에틸아세테이트 분획에서만 유의성을 나타내었다(p>0.05). 인삼 중 항산화 활성은 에틸아세테이트 층으로 이행되는 폴리페놀 계통 성분이나 일부 비극성의 사포닌에 의한 것으로 추측되고 있으나 모든 분획에서 나타난 것으로 보아 이들 외에 산성 다당체, 당 단백질, 수용성 다당류 등 다른 생리활성 물질에 대한 연구가 요구된다.

• Natural Product Sciences
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• 제20권3호
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• pp.176-181
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• 2014

The antioxidant activities of 29 components isolated from the aerial parts of Artemisia iwayomogi were evaluated in vitro and in cell culture. Among the tested compounds, 2, 6, 8, 10, 13, and 14 exhibited the greatest peroxyl radical-scavenging activities in the oxygen radical absorbance capacity (ORAC) assay, and 2, 10, and 14 also showed significant reducing capacities. However, all compounds showed weak metal chelating activities. Their cellular antioxidant activities were evaluated in HepG2 cells. At $10{\mu}M$, compounds 6, 8, and 14 exhibited stronger protection against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress than compounds 2, 10, and 13. Moreover, Compounds 2 and 8 were more effective in protecting against $Cu^{2+}$-induced oxidative stress than compounds 6, 10, 13, and 14 at $10{\mu}M$. These results suggest that the phenolic compounds in A. iwayomogi have the potential to be developed as natural antioxidants for the treatment of oxidative stress-related diseases.

• 한국식품과학회지
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• 제46권3호
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• pp.364-368
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• 2014

양파가공부산물인 양파껍질을 이용하여 산업체에서 활용 가능한 추출공정으로 다양한 항산화활성 변화를 확인 하였다. 양파껍질 추출물의 총 폴리페놀 함량은 OPME-1에서 233.90 mg/g으로 가장 높게 나타났으며, DPPH법에 의한 항산화활성의 $IC_{50}$값은 OPMPE-1에서 1.15 mg/mL로 가장 높은 radical 소거활성을 나타났다. ABTS에 의한 총 항산화력은 OPM에서 432.78 mg AA eq/g으로 높은 항산화활성을 나타냈으며, 환원력은 OPMPE-1에서 1.69 $A_{700}$으로 높게 나타났고, ORAC 지수는 OPMPE-1에서 $318,509{\mu}M\;TE/mL$로 가장 높게 나타났다. 본 연구결과 실제 산업체에서 양파껍질 추출물을 제조할 때 활용 가능한 추출공정은 여러 번 반복 추출을 하는 것보다 1회 추출만 하는 공정이 항산화 활성 결과 기능성 식품소재 원료로 활용이 더 유리할 것으로 사료된다.

• Natural Product Sciences
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• 제18권4호
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• pp.261-265
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• 2012

Xanthohumol was isolated from hops (Humulus lupulus L.), and then investigated anti-oxidant effect by AAPH-induced LLC-PK1 cell and oxygen radical absorbance capacity (ORCA) assays and MMP-12 inhibitory effect by direct MMP-12 inhibition assay. The treatment of xanthohumol protected LLC-PK1 cells from AAPH-induced cell damage such as cell viability, SOD and GSH-px reduction in a dose dependant manner (0.1, 1, and $5{\mu}M$), the SOD value was 2.98, 4.51, and 5.77 U/mg protein, and GSH-px value was 30.12, 49.32, and 60.11 U/mg protein. ORAC value of xanthohumol was showed as 4320, 12004, and $14209{\mu}M$ TE/g at the concentration 0.1, 1, and $5{\mu}M$, respectively. The change of SOD and GSH-px values was significantly correlated with the results of ORAC assay, that is, AAPH-induced cell and ORCA assays. In addition, inhibition of MMP-12 that is known to play an important role in skin aging was 14%, 37%, 46%, and 79% at the concentration of 0.01, 0.1, 1, and $5{\mu}M$, respectively. On the basis of these results, xanthohumol from hops (Humulus lupulus L.) showed interesting biological and pharmacological activity such as anti-oxidant effect and anti-aging.

• 한국조리학회지
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• 제24권2호
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• pp.103-111
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• 2018

The objectives of the present study were to examine the antioxidant activity of autofocusing fractions from egg white protein hydrolysates and obtain higher antioxidant peptide fraction, which could be applied to the food model system. Alkaline protease hydrolysate of egg white protein exerted higher antioxidant activities than other protein hydrolysates and were fractionated on the basis of the amphoteric nature of sample peptides by preparative isoelectric focusing without toxic solvents and reagents, which is termed autofocusing. Neutral and basic fractions showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the acidic fractions. The acidic and neutral fractions showed higher hydroxyl (OH) radical scavenging activity and oxygen radical absorbance capacity (ORAC) values than the basic fractions. The acidic fractions showed higher metal chelating activity than basic fractions. Antioxidant activities of some autofocusing fractions except for ORAC showed higher compared to the crude hydrolysate. These results suggest that peptides fractions from egg white protein are effective antioxidant, and that autofocusing could be useful to increase antioxidant activity for application to food system.

• 한국식품영양과학회지
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• 제42권7호
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• pp.997-1002
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• 2013

본 연구에서는 음료 농축액 및 퓨레로 사용되는 석류 농축액, 블루베리 농축액, 양파추출 농축액, 도라지추출 농축액, 흑마늘 농축액, 홍삼 농축액, 아사이베리 퓨레, 새싹유채 퓨레의 이화학적 특성, 총 페놀 함량, 항산화 효능(DPPH radical scavenging, ORAC) 및 아질산염 소거능을 분석하였다. pH는 석류 농축액이 2.81로 가장 낮았으며 양파추출 농축액이 5.12로 가장 높은 수치를 나타냈다. Brix는 홍삼 농축액이 70.30으로 가장 높았고 새싹유채 퓨레가 1.70으로 가장 낮았다. 총 페놀 함량은 아사이베리 퓨레에서 182.71 mg GAE/mL로 다른 시료에 비하여 높은 수치를 나타냈고 양파추출 농축액이 8.80 mg GAE/mL로 가장 낮았다. DPPH 라디칼 소거능 측정은 총 페놀 함량과 마찬가지로 아사이베리 퓨레에서 69.88%로 가장 높은 활성을 나타내었다. ORAC 지수는 블루베리 농축액에서 $27,514{\mu}M$ TE/mL로 가장 높게 나타났으며 아질산염 소거능도 아사이베리 퓨레에서 28.19%로 유의적으로 높은 활성을 보였다. 이상의 연구 결과로부터 천연물 유래 농축액 및 퓨레는 다양한 항산화 평가모델에서 항산화 활성을 나타내었으며 이들 농축액 및 퓨레를 이용한 항산화 활성을 갖는 건강음료의 원료로서 활용이 가능할 것으로 사료된다.