• 제목/요약/키워드: Oxygen Radical Absorbance Capacity (ORAC)

검색결과 96건 처리시간 0.022초

In Vitro and Cellular Antioxidant Activity of a Water Extract of Saururus chinensis

  • Kim, Gyo-Nam;Lee, Jung-Sook;Jang, Hae-Dong
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1332-1336
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    • 2008
  • The water extract of Saururus chinensis was investigated for oxygen radical absorbance capacity (ORAC), reducing capacity, metal chelating activity, and intracellular antioxidant activity using HepG2 cell. When 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) was used for the generation of peroxyl radicals in vitro, S. chinensis extract (SC-E) showed the strong and concentration-dependent scavenging activity through donating protons which could be explained by its reducing property. When hydroxyl radicals were generated in vitro through the addition of $Cu^{2+}$ and $H_2O_2$, SC-E demonstrated the antioxidant activity depending on its concentration. In HepG2 cell model, most of intracellular oxidative stress generated by AAPH was efficiently removed by SC-E. However, when $Cu^{2+}$ without $H_2O_2$ was used as an oxidant in the intracellular assay, SC-E partially reduced the oxidative stress caused by $Cu^{2+}$ in cellular antioxidant activity assay system. These results indicate that SC-E could be utilized for the development of functional foods as antioxidant resource in the near future.

솔잎착즙액을 첨가한 설기떡의 가열조리에 따른 항산화 및 항유전독성 효능 검증 (Changes in the Antioxidative and Antigenotoxic Effects After the Cooking Process of Sulgidduk Containing Pine Needle Juice)

  • 이현정;김소윤;박재희;김래영;정현숙;박은주
    • 한국식품조리과학회지
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    • 제29권5호
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    • pp.453-462
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    • 2013
  • Recently, two formulas of sulgidduk added to pine needle juice (PNJ) with various physiological activities were developed for metabolic syndrome patients in our lab. According to previous studies, cooking may alter antioxidant properties by initiating destruction, release or transformation of antioxidant compounds contained in food. Therefore, the aim of this study was to compare the antioxidant activities and antigenotixic effects of sulgidduk with/without PNJ and to note changes in these activities after cooking. The ingredients of sulgidduk was added on the basis of 100% rice flour as follows: conventional sulgidduk (S): 1.5% salt, 30.0% sugar; PNJ added to sulgidduk A (PS-A): 1.4% salt, 30.0% sugar, and 1.0% PNJ; PNJ added to sulgidduk B (PS-B): 1.5% salt, 21.4% sugar, and 1.4% PNJ. Ethanol and water extracts of sulgidduk were analyzed for the total phenolic content (TPC), DPPH radical scavenging activity (DPPH RSA), total radical-trapping antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), and antigenotoxic effect by comet assay. The ethanol extracts PS-A and PS-B showed higher TPC and antioxidant activities (DPPH RSA, TRAP, and ORAC) than did the S ethanol extract before cooking. The more PNJ was added, the higher TPC and anitoxidant activities were observed in sulgidduk (PS-A$200{\mu}M$ of $H_2O_2$. Taken together, this study suggests that sulgidduk added to 1.44% of pine needle juice may be a good option antioxidant and antigenotoxic source.

사리장의 항산화 효과 (In vitro Antioxidant Effects of Sarijang)

  • 서보영;최미주;최은아;박은주
    • 한국식품영양과학회지
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    • 제43권4호
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    • pp.618-623
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    • 2014
  • 본 연구에서는 서목태를 기본으로 하여 제조된 전통 발효물인 사리장의 항산화 활성 분석 및 comet assay를 이용한 DNA 손상 억제 효과를 분석하고자 하였다. 사리장의 총 폴리페놀 함량은 $1.04{\pm}0.01$ mg GAE/mL로 나타났다. 항산화 활성을 분석한 DPPH 라디칼 소거능 및 TRAP는 농도 의존적으로 활성이 증가하였으며, 각각의 $IC_{50}$은 11.2 mg/mL와 1.2 mM로 나타났다. ORAC 활성 역시 농도 의존적 증가 활성을 나타내었다. 세포의 ROS 소거능(CAC)은 사리장 처리구의 모든 농도(10~100 ${\mu}g/mL$)에서 NC와 동일한 수준의 ROS 억제 활성을 나타내었다. Comet assay를 이용한 DNA 손상 보호 효과는 $H_2O_2$, Fe-NTA 그리고 HNE에 의한 산화적 스트레스에 의한 DNA 손상을 농도 의존적으로 보호하는 것으로 나타났으며, $IC_{50}$$H_2O_2$ 처리군이 13.4 ${\mu}g/mL$, Fe-NTA 처리군이 32.2 ${\mu}g/mL$, HNE 처리군이 59.9 ${\mu}g/mL$로 나타났다. 이상의 결과들은 사리장이 항산화 관련 생리활성을 가지는 것으로 판단되며, 향후 사리장에 포함된 생리활성 성분의 탐색과 in vivo 모델을 통한 생리활성 연구가 이루어져야 할 것으로 보인다.

Total antioxidant capacity of the Korean diet

  • Han, Jeong-Hwa;Lee, Hye-Jin;Cho, Mi Ran;Chang, Namsoo;Kim, Yuri;Oh, Se-Young;Kang, Myung-Hee
    • Nutrition Research and Practice
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    • 제8권2호
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    • pp.183-191
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    • 2014
  • BACKGROUND/OBJECTIVES: The objective of this study was to measure and/or estimate the total antioxidant capacity of the Korean diet. MATERIALS/METHODS: Eighty-one plant foods that were expected to exhibit rather high antioxidant activities were selected from the Korean diet using the Fifth Korean National Health and Nutrition Survey (KNHANES V). These foods were categorized into 11 food groups: cereals, potatoes, legumes, nuts, vegetables, kimchies, mushrooms, fruits, fruit juices, sea weeds, and oils. The foods were mixed in the proportions specified in traditional Korean recipes and analyzed. The measured indicators for antioxidant capacities were total phenolics, 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and Trolox equivalent antioxidant capacity (TEAC). RESULTS: Total phenolics were high in the fruit juices, nuts, vegetables, and fruits; and the average DPPH, ORAC, and TEAC values were high in the vegetables, fruits, fruit juices, and nuts. The correlation coefficient between the content of total phenolics of each food and the in vitro antioxidant capacity was relatively high at 0.851. The intake of total phenolics per capita per day in the Republic of Korea was estimated to be 127 mg. The total dietary antioxidant capacity (TDAC) values, which were obtained from the total antioxidant capacity of each food, taking into account the intake of each food, were 20,763, 54,335, and $876.4{\mu}mol$ of Trolox equivalents using the DPPH, ORAC, and TEAC methods, respectively. The food group that contributed the most to the Korean TDAC was cereals at 39.7%, followed by fruits and vegetables at 27.8% and 13.9%, respectively. The contribution of legumes, nuts, fruit juices, and mushrooms was quite minimal at less than 2% each. CONCLUSIONS: The content of total phenolics and the antioxidant capacity of the Korean diet are significantly correlated and the high contributing food groups are cereals, fruits, and vegetables.

한국 전통 간장의 항산화 활성 (Antioxidant Activity of Korean Traditional Soy Sauce)

  • 이상기;정윤화;임성빈;유성률
    • 한국식품영양과학회지
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    • 제44권9호
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    • pp.1399-1406
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    • 2015
  • 본 연구에서는 전국 17지역에서 생산한 한국 전통 간장의 이화학적 분석, 색도 및 갈색도, 총 질소 및 아미노태 질소, 총 폴리페놀의 함량, protease 활성과 항산화 활성을 측정하고, 이들 사이의 상관관계를 분석하였다. pH는 4.34~5.37, 염도는 16.77~34.71%, 환원당은 3.00~32.04 mg/mL, 총 고형분 함량은 19.95~36.86%, 가용성 고형분 함량은 23.10~41.70oBrix의 범위를 보였다. 총 질소 함량은 무주(JMJ) 간장이 12.60%로 가장 높았고, 평창(GPC) 간장이 2.23%로 가장 낮았다. 아미노태 질소는 JMJ 간장에서 139.30mg%로 가장 높은 함량을 보였고, GPC 간장에서 28.23mg%로 가장 낮은 함량을 보였다. Protease 활성은 JMJ 간장에서 2.20 U/mL로 가장 높은 활성을 나타냈고, GPC 간장에서 0.35 U/mL로 가장 낮은 활성을 나타냈다. 총 폴리페놀 함량은 JMJ 간장에서 11.20 GAE mg/mL로 가장 많이 포함되었고, GPC 간장에서 2.37 GAE mg/mL로 가장 적게 포함되었다. DPPH 라디칼 소거 활성은 JMJ 간장에서 63.39%로 가장 높은 활성을 보였고, GPC 간장에서 8.96%로 가장 낮은 활성을 보였다. FRAP는 JMJ 간장에서 2.41 AE mg/mL로 가장 높은 활성을 보였고, GPC 간장에서 0.12 AE mg/mL로 가장 낮은 활성을 보였다. ORAC는 JMJ 간장에서 115.69 TE mg/mL로 가장 높은 항산화 활성을 보였고, GPC 간장에서 8.42 TE mg/mL로 가장 낮은 항산화 활성을 보였다. 실험항목 간 상관관계를 본 결과 protease 활성이 총 폴리페놀 함량과 0.995로 가장 높은 상관성을 보였고, 총 폴리페놀 함량은 DPPH 라디칼 소거 활성과 0.973으로 상관성이 높았다.

삼채의 잎, 인경, 뿌리의 총페놀 함량, 총플라보노이드 함량 및 항산화능 (Total Phenolics, Total Flavonoids, and Antioxidant Capacity in the Leaves, Bulbs, and Roots of Allium hookeri)

  • 황정승;이봉한;안향설;정하람;김영은;이인일;이형재;김대옥
    • 한국식품과학회지
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    • 제47권2호
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    • pp.261-266
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    • 2015
  • 삼채의 잎, 인경, 뿌리 세 부위를 물, 메탄올-물 혼합용매, 무수 메탄올을 이용하여 추출물을 제조한 후, 총페놀 함량, 총플라보노이드 함량 및 항산화능을 정량 분석하였다. 삼채의 부위별 총페놀 함량은 잎이 240.4-276.6 mg GAE/100 g FW, 인경이 65.5-82.8 mg GAE/100 g FW, 뿌리가 50.0-59.4 mg GAE/100 g FW를 보였다. 삼채의 부위별 총플라보노이드 함량은 잎에서 9.7-34.1 mg CE/100 g FW, 인경에서 5.0-16.7 mg CE/100 g FW, 뿌리에서는 4.4-15.3 mg CE/100 g FW로 나타났다. ABTS법, DPPH법, ORAC법을 통한 항산화능은 잎에서 각각 78.7-103.4, 24.4-59.0, 1,798.8-2,169.7 mg VCE/100 g FW, 인경에서는 35.8-51.1, 9.3-26.3, 415.3-611.7 mg VCE/100 g FW, 뿌리에서는 42.0-55.9, 12.8-24.4, 291.7-429.4 mg VCE/100 g FW을 보였다. 삼채 잎, 인경, 뿌리 중에서 잎이 가장 높은 총페놀 함량, 총플라보노이드 함량 및 항산화능을 가졌다. 6가지 추출용매에서 60% (v/v) 메탄올-물 혼합용매에서 주로 높은 활성을 나타냈다. 항산화능은 총플라보노이드 함량보다 총페놀 함량과 더 높은 상관관계를 보였다. 본 연구의 결과는 삼채가 높은 항산화능 공급할 수 있는 원료로서의 가능성을 보여 주었다는데 의의가 있다.

Hyaluronidase Inhibitory and Antioxidant Activities of Enzymatic Hydrolysate from Jeju Island Red Sea Cucumber (Stichopus japonicus) for Novel Anti-aging Cosmeceuticals

  • Ding, Yuling;Jiratchayamaethasakul, Chanipa;Kim, Eun-A;Kim, Junseong;Heo, Soo-Jin;Lee, Seung-Hong
    • 한국해양바이오학회지
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    • 제10권2호
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    • pp.62-72
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    • 2018
  • An active ingredient with hyaluronidase (HAse) inhibitory effect is one of the anti-aging approaches in cosmeceuticals. Here, red sea cucumbers (RSCs), Stichopus japonicus, from Jeju Island were evaluated to examine their HAse inhibitory and antioxidant activity effect. In this study, RSCs were extracted by six enzymatic hydrolysis (Alcalase; Al, Trypsin; Try, Neutrase; Neu, Pepsin; Pep, Alpha-chymotrypsin; Chy and Protamex; Pro). Alcalase hydrolysate (AlH) showed the highest antioxidant capacities for both of oxygen radical absorbance capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) methods, compared to those of other hydrolysates, at $66.59{\pm}0.78{\mu}M\;TE/mg$ and $135.78{\pm}3.24{\mu}M\;TE/mg$, respectively. Furthermore, AlH performed the highest capacity of HAse inhibitory with $IC_{50}$ value of 3.21 mg/ml. Thus, RSCs hydrolyzed by Al were chosen to determine the cellular antioxidant activity and hyaluronic acid (HA) production effect on Human immortalized keratinocyte cell line (HaCaT). The results showed that AlH improved the cell viabilities and intracellular reactive oxygen species (ROS) induced by 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) were significantly decreased. In addition, AlH increased HA amount by regulating HYAL2 and HAS2 expressions in the HaCaT cells. Taken together, AlH of RSCs collected from Jeju Island showed HAse inhibitory and antioxidant activities against skin-aging which shows its potentials can be an optional natural bioactive ingredient for novel cosmeceuticals.

Optimization of Mixing Ratio to Improve Antioxidant Activity of Aged Garlic with Pine Needle Using Response Surface Methodology

  • Park, Jae-Hee;Shin, Eunji;Park, Eunju
    • Preventive Nutrition and Food Science
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    • 제22권3호
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    • pp.241-245
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    • 2017
  • Aged garlic has been reported to possess beneficial pharmacological activities, including anti-stress and anti-fatigue properties, and to exert protective effects on the cardiovascular system and liver. Pine needles are widely used in folk medicine and as food additives owing to their pharmacological properties such as anti-aging and anti-inflammatory effects. It has long been known that combining certain phytochemicals with other phenols or organic acids can produce synergistic effects. Therefore, the purpose of this study was to develop an optimal formula of aged garlic with added pine needle powder for improved antioxidant activity using the statistical technique of response surface methodology. The antioxidant activities of aged garlic mixed with pine needle powder were confirmed by measuring oxygen radical absorbance capacity and total polyphenol content. An optimized antioxidant formula was identified that contained 5.08 g aged garlic and 1.97 g pine needle powder. The antioxidant activities of the mixture prepared using this optimal formula were significantly higher than the predicted values according to an additive model. Hence, this study confirms that the addition of pine needle powder to aged garlic can improve its antioxidant activity. This study demonstrated an optimal mixing ratio to produce an aged garlic product with improved functionality through the addition of pine needle powder that could be successfully employed by the food industry to prepare functional foods.

Phenolic Composition and Antioxidant Activities of Different Solvent Extracts from Pine Needles in Pinus Species

  • Kang, Yoon-Han;Howard, Luke R.
    • Preventive Nutrition and Food Science
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    • 제15권1호
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    • pp.36-43
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    • 2010
  • The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.

산화적 손상에 대한 키조개(Atrina pectinata) 효소 가수분해물의 간세포 보호 효과 (Cytoprotective Effect of a Neutrase Enzymatic Hydrolysate Derived from Korea Pen Shell Atrina pectinata Against Hydrogen Peroxide -Induced Oxidative Damages in Hepatocytes)

  • 한의정;신은지;김기웅;안긴내;배태진
    • 한국수산과학회지
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    • 제53권1호
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    • pp.123-131
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    • 2020
  • In this study, we investigated the protective effects of a Neutrase enzymatic hydrolysate derived from Korea pen shell Atrina pectinata (APN) against hydrogen peroxide (H2O2)-induced oxidative damage in hepatocytes. First, we confirmed that APN has antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS+) and H2O2 and increasing oxygen radical absorbance capacity (ORAC) value. Also, the treatment of APN increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H2O2-stimulated hepatocytes. In addition, APN decreased the sub-G1 DNA contents and the apoptotic body formation increased by H2O2 stimulation. Moreover, APN modulated the protein expression of apoptosis related molecules (Bcl-2, Bax and p53) by suppressing the activation of nuclear factor NFkB and ERK/p38 signaling in H2O2-stimulated hepatocytes. Furthermore, APN led to the activation of Nrf2/HO-1signaling known as antioxidant systems. These results suggest APN protects hepatocytes against oxidative damages caused by H2O2 stimulation.