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http://dx.doi.org/10.5657/KFAS.2020.0123

Cytoprotective Effect of a Neutrase Enzymatic Hydrolysate Derived from Korea Pen Shell Atrina pectinata Against Hydrogen Peroxide -Induced Oxidative Damages in Hepatocytes  

Han, Eui Jeong (Department of Research and Development)
Shin, Eun-Ji (Department of Food Technology and Nutrition, Chonnam National University)
Kim, Kee-Woong (Department Marine Bio-Food Sciences, Chonnam National University)
Ahn, Ginnae (Department of Food Technology and Nutrition, Chonnam National University)
Bae, Tae Jin (Department of Food Technology and Nutrition, Chonnam National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.1, 2020 , pp. 123-131 More about this Journal
Abstract
In this study, we investigated the protective effects of a Neutrase enzymatic hydrolysate derived from Korea pen shell Atrina pectinata (APN) against hydrogen peroxide (H2O2)-induced oxidative damage in hepatocytes. First, we confirmed that APN has antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS+) and H2O2 and increasing oxygen radical absorbance capacity (ORAC) value. Also, the treatment of APN increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H2O2-stimulated hepatocytes. In addition, APN decreased the sub-G1 DNA contents and the apoptotic body formation increased by H2O2 stimulation. Moreover, APN modulated the protein expression of apoptosis related molecules (Bcl-2, Bax and p53) by suppressing the activation of nuclear factor NFkB and ERK/p38 signaling in H2O2-stimulated hepatocytes. Furthermore, APN led to the activation of Nrf2/HO-1signaling known as antioxidant systems. These results suggest APN protects hepatocytes against oxidative damages caused by H2O2 stimulation.
Keywords
Atrina pectinate; Hydrogen peroxide; Oxidative damages; Hepatocytes;
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