• Natural Product Sciences
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• 제18권4호
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• pp.261-265
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• 2012

Xanthohumol was isolated from hops (Humulus lupulus L.), and then investigated anti-oxidant effect by AAPH-induced LLC-PK1 cell and oxygen radical absorbance capacity (ORCA) assays and MMP-12 inhibitory effect by direct MMP-12 inhibition assay. The treatment of xanthohumol protected LLC-PK1 cells from AAPH-induced cell damage such as cell viability, SOD and GSH-px reduction in a dose dependant manner (0.1, 1, and $5{\mu}M$), the SOD value was 2.98, 4.51, and 5.77 U/mg protein, and GSH-px value was 30.12, 49.32, and 60.11 U/mg protein. ORAC value of xanthohumol was showed as 4320, 12004, and $14209{\mu}M$ TE/g at the concentration 0.1, 1, and $5{\mu}M$, respectively. The change of SOD and GSH-px values was significantly correlated with the results of ORAC assay, that is, AAPH-induced cell and ORCA assays. In addition, inhibition of MMP-12 that is known to play an important role in skin aging was 14%, 37%, 46%, and 79% at the concentration of 0.01, 0.1, 1, and $5{\mu}M$, respectively. On the basis of these results, xanthohumol from hops (Humulus lupulus L.) showed interesting biological and pharmacological activity such as anti-oxidant effect and anti-aging.

• 분석과학
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• 제8권3호
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• pp.281-284
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• 1995

개정된 공정 시험법의 세부 절차상의 변경이 시험 결과에 미치는 영향을 조사하였다. 가열 시간이 1/3로(60분${\rightarrow}$20분), 과망간산칼륨의 농도가 1/2.5(0.025N${\rightarrow}$0.010N)로 줄어든 결과로 글루코스의 경우 COD로서의 회수율이 평균적으로 약 30% 감소하였다. 즉, 개정된 절차에 따른 시험 결과는 개정 전의 절차에 의한 결과의 70%밖에 안 된다. 이 때 정밀성은 다소 향상되었다(CV가 4.6%${\rightarrow}$3.3%). 이러한 $KMnO_4$의 산화능 감소는 주로 농도의 묽어짐 때문이었다. 나머지 감소 효과의 1/3이 가열 시간 감소의 영향으로 드러났다. 이처럼 알칼리성 COD법에서는 산화제의 농도 변화가 가열 시간의 경우보다 결과에 미치는 영향이 훨씬 큼을 나타내었다. 실제 바닷물 시료에서도 같은 결과를 얻을 수 있었다.

• 융합정보논문지
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• 제11권9호
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• pp.115-123
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• 2021

본 연구는 산말 3종류 Desmarestia dudresnayi subsp. Tabacoides (담배잎산말), Desmarestia viridis (쇠꼬리산말), Desmarestia ligulate (참산말) 잎 추출물의 항산화 및 항 멜라닌 활성에 대한 기능적 연구를위해 수행하였다. 3종류의 산말 중 담배잎산말 추출물이 2.5 mg / mL의 농도에서 DPPH 및 ABTS 라디칼 소거활성이 각각 68.0 % ± 1.9 % 및 84.6 % ± 1.7 %로 강한 항산화력을 나타냈다. 질소 (NO) 라디칼 소거 활성은 91.6 % ± 1.1 %의 높은 항산화능을 보였다 산말 추출물을 이용한 B16F10세포의 세포독성은 100 ㎍/mL 농도에서 3종의 산말 추출물이 모두 85% 이상의 세포 생존률을 보였고, 100 ㎍ / mL농도에서 담배잎산말 및 쇠꼬리산말이 70% 이상의 멜라닌 억제효과를 보였다. 이러한 내용은 산말이 항산화 및 항멜라닌 활성과 같은 천연 화장품 소재로 사용될 수 있을 것으로 사료된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 하계학술대회 논문집
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• pp.859-862
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• 2001

This study describes a selective Ag etching solution for use with pattern on the surface of copper. This etching solution uses potassium iodide and potassium sulfate as the ligand that coordinates to the metal ions and ferricyanide as the oxidant. The etching rate was depended on the concentration of co-ligands and time. But the etching rate wasn't depended on the pH(2∼6), and oxidant(K$_3$Fe(CN)$\_$6/). Complete etching of silver can be achieved rapidly within 90sec for 4.46${\mu}$m thick metal films when aqueous solutions containing K$_3$Fe(CN)$\_$6/, K$_2$S$_2$O$\_$8/ and KI was used. This etching solution was characteristic of anisotropic etching.

• KSBB Journal
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• 제31권1호
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• pp.14-19
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• 2016

To investigate the effect of peanut sprout extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of peanut sprout extract, there was strongly scavenging activity. Fluorescent material DCF-DA was used to measure hydrogen peroxide created in RAW 264.7 cells, and all concentration dependently decreased ROS production. As a result of measuring nitric oxide to examine anti-inflammation of peanut sprout extract, there was strongly inhibited nitric oxide production in RAW 264.7 cells. Tyrosinase activation was found to inhibited dose-dependant. Melanin production was also prevented dose-dependant. Therefore, it is expected to be used effectively in development of functional cosmetic materials.

• 한국농공학회논문집
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• 제64권5호
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• pp.1-8
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• 2022

Hydrogen can be produced by gasification of biomass and other combustible fuels. Depending on oxydant agents, syngas or producer gas compositions become quite different. Since biomass has limited amount of hydrogen including moisture in it, the hydrogen concentration in the syngas is about 15% when air is supplied for oxidant agent. Experiments were conducted to investigate the channges in hydrogen concentrations in syngas with different oxidant agent conditions, fuel conditions, and external heat supply. Allothermal reaction resulted in higher concentrations of hydrogen with the supply of steam over air, reaching over 60%. Hydrogen is produced by water-gas and water-gas shift reactions. These reactions are endothermic and require enough heat. Autothermal reaction occurred in the downdraft gasifier used in the experiment did not provide enough heat in the reactions for hydrogen production. Steam seems a more desirable oxidant agent in producing the syngas with higher concentrations of hydrogen from biomass gasifications since nitrogen is included in syngas when air is used.

• 한국의류학회지
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• 제36권8호
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• pp.875-884
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• 2012

Many Koreans have recently bleached their black hair to blond or a light color due to the influence of their favorite Korean idols. Bleaching effects were studied under various bleaching conditions, such as the concentration of oxidants, the ratio between bleach accelerant and oxidant, and treatment temperature and time. The degree of damage of bleached hair with the same color difference (${\Delta}E$) intervals was observed in SEM images, the retention of breaking load and the change of color after dyeing. The ratio between bleach accelerant and oxidant of 1:2.5 was an effective condition. $L^*$ values of the bleached hair treated with 9% oxidant increased to 90 min. At a treatment temperature of $30^{\circ}C$, $a^*$ values were maximum at 30 min and then decreased as time further increased; however, $b^*$ values of the bleached hair increased as the treatment time increased. At $45^{\circ}C$, both of $a^*$ and $b^*$ values showed a maximum at a specific time. Bleaching efficiency was influenced most by temperature, followed in order by time, and oxidant concentration. As the treatment time increased, $L^*$ values greatly increased with twice treatment; however, $a^*$ and $b^*$ values decreased. Bleached hair, which had a color difference of higher than 30, showed the scales were completely removed and the retention of the breaking load greatly decreased. Highly bleached hair showed a great decrease in $L^*$ values by dyeing; however, dyeing with bright colors was more indicative to the effect of bleaching.

• 한방안이비인후피부과학회지
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• 제33권1호
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• pp.1-11
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• 2020

Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung-tang(OST) extract. Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400㎍/㎖) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400㎍/㎖) was investigated by measuring DPPH scavenging activity. Results : OST extract showed a cell survival rate of 90% or more at 50-400㎍/㎖. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). It was found that OST has a high free-radical scavenging ability. Conclusions : These results suggest that OST extract can be used as a treatment for various skin diseases by demonstrating its anti-inflammatory and anti-oxidant effects.

• 대한한방부인과학회지
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• 제21권4호
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• pp.69-89
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• 2008

Purpose: This study was performed to evaluate anti-oxidant activities and anti-inflammatory effects of Sungyoutanggagambang(SYTG). Methods: In the study of anti-oxidant activities. SYTG was investigated by DPPH radical scavenger activity. superoxide dismutase activity and superoxide anion radical scavenger activity. In the study of anti-inflammatory effects. SYTG was investigated using cultured cells and murine models. As for the parameters of inflammation. levels of several inflammatory cytokines and chemical mediators which are known to be related to inflammation were measured in mouse lung fibroblast cells(mLFCs) and RAW264.7 cells. Results: Prior to the experiment. we investigated the security of SYTG by measuring GOT and GPT in serum. 1. SYTG showed high antioxidant activity in a concentration-dependent degree by measured scavenging activity of DPPH free radical, superoxide dismutase and superoxide anion radical. 2. SYTG inhibited IL-1$\beta$, IL-6. TNF-$\alpha$, COX-2 and NOS-II mRNA expression as compared with the control group in a concentration-dependent degree in RAW264.7 cell line. 3. SYTG inhibited IL-1$\beta$, IL-6 production significantly at 100 ${\mu}g/ml$ and TNF-$\alpha$ production significantly at 50, 100 ${\mu}g/ml$ as compared with the control group in RA W264.7 cell line. 4. SYTG inhibited IL-1$\beta$, and IL-6 production significantly as compared with the control group in serum of acute inflammation-induced mice. and decreased IL-1$\beta$, IL-6 production in spleen tissue. and also decreased IL-1$\beta$, IL-6 production in liver tissue. Conclusion: These results suggest that SYTG can be useful in treating diverse female diseases caused by inflammation such as endometrosis, myoma, pelvic congestion. chronic cervicitis, chronic pelvic inflammatory disease and so on.

• KSBB Journal
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• 제28권5호
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• pp.327-331
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• 2013

This study aimed to observe the effects of Sea Buckthorn seed extracts (SEB) on anti-oxidation and antiinflammation. SEB were found to strongly depress ROS production depending on concentration, and 46.5% anti-oxidation effects were found in the top concentration of 100 ${\mu}g/mL$. Histamine and arachidonic acid release were measured to observe the effects of SEB on anti-inflammation. SEB inhibited histamine release and arachidonic acid release on dose dependent manner. Based on the results above, the conclusion is drawn that SEB have the potential to be used as natural materials that are effective for anti-oxidation and antiinflammation.