• Title/Summary/Keyword: Oxalis

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Development of a diagnostic system to detect potato virus T using RT-PCR and nested PCR (감자T바이러스 검정을 위한 RT-PCR 및 Nested PCR 진단시스템 개발)

  • Lee, Si Won;Shin, Yong-Gil;Lee, Jin-Young;Kim, Young-Suk;Yang, Mi Hee;Choi, In-Cheol
    • Korean Journal of Agricultural Science
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    • v.42 no.2
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    • pp.99-103
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    • 2015
  • Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 ($734{\rightarrow}315bp$) and PVT-N75/C30 ($828{\rightarrow}529bp$)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.

Disturbance, Diversity, Regeneration and Composition in Temperate Forests of Western Himalaya, India

  • Tiwari, Om Prakash;Sharma, Chandra Mohan;Rana, Yashwant Singh;Krishan, Ram
    • Journal of Forest and Environmental Science
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    • v.35 no.1
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    • pp.6-24
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    • 2019
  • We have investigated the impact of anthropogenic and natural disturbances on regeneration, composition and diversity in some temperate forests of Bhagirathi Catchment Area of Garhwal Himalaya. The forests were categorized on the basis of canopy cover and magnitude of disturbance into highly, moderately and least disturbed classes. The dominant tree species at lower elevation were Pinus roxburghii and Quercus leucotrichophora, while Abies pindrow, Q. semecarpifolia and Rhododenron arboreum were the dominant species at the upper elevational forests. Cythula tomentosa and Indegophera heterentha were the dominant shrub species present in all the forests. Similarly, Circium wallichii and Oxalis corniculata were the dominant herb species found in all forests (except Q. leucotrichophora forest), whereas Thalictrum foliolosum and Viola pilosa were noticed in each forest (except P. roxburghii forest). The tree density values oscillated between $400{\pm}10\;trees\;ha^{-1}$ to $750{\pm}89.1\;trees\;ha^{-1}$ which generally decreased from lower to higher disturbance regimes however, the total basal cover value was highest ($88.1{\pm}23.6m^2\;ha^{-1}$) in highly disturbed forest and lowest ($25.8{\pm}2.2m^2\;ha^{-1}$) in moderately disturbed forest. The shrub and herb densities were maximum in least disturbed forest, while the young regenerating individuals i.e., sapling and seedling were observed increasing from high to low disturbed forests which reflected that the forest fragmentation adversely affected the regeneration. However, A. pindrow and P. roxburghii were found invariably encroaching the habitats of R. arboreum and Q. leucotrichophora at various altitudes, respectively. The Canonical Correspondence Analysis clearly indicated that the elevation and lopping intensity have more impact on trees, while shrub and herbs were more influenced by elevation, canopy cover, light attenuation and soil erosion. Pinus roxburghii was the only species which was affected by heavy litter removal and forest fire.

Weed Flora of Range Area in Cheju Island in Korea (제주도(濟州道) 목초지(牧草地)에 발생(發生)하는 잡초종(雜草種))

  • Kim, K.U.;Kim, S.H.;Kim, C.G.;Kim, J.H.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.8 no.3
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    • pp.291-294
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    • 1988
  • About 136 weed species belonging to 44 families occurring in summer observed in Cheju island. The Compositae was the most widely occurring family covering 29 weed species, followed by 16 species in Graminae, 14 in Leguminosae, 13 in Rosaceae, 10 in Polygonaceae etc., The dominant weed species detected in range areas of Cheju island were Trifolium repens, Botrychium virginianum, Rosa multiflora, Miscanthus sinensis, Imperata cylindrica. The range areas of Cheju island consisted of the communities of Artemisia princeps-Botrychium virginianum, Artemisia princeps-Erigeron borariensis and Dianthus sinensis, and Artemisia-Lespedeza bicolor and Miscanthus sinensis and then the differential species of their communities were Botrychium virginianum, Erigeron boriensis, and Lespedeza bicolor and Miscanthus sinensis, respectively. The species of the high frequencies occurring in investigated sites were Artemisia princeps, Cirsium rhinoceros, Hydrocotyle ramijlora, Rosa multiflora, Sanguisorba officinalis, Rubus crataegifolius, Potentilla fragariodes, Erigeron annus, Plantago asiatica, Oxalis corniculata, Mosla dianthera in order.

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Ecological Characteristics of Viola websteri Hemsley Habitats (왕제비꽃 자생지의 생태특성)

  • Jang, Su-Kil;Cheon, Kyeong-Sik;Kim, Kyung-Ah;Jang, Jin-Hwan;Yoo, Ki-Oug
    • Korean Journal of Plant Resources
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    • v.23 no.4
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    • pp.261-273
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    • 2010
  • This study intended to investigate the environmental factors including soil and vegetation in order to understand the environmental and ecological characteristics of seven different habitats of Viola websteri. These habitats, according to investigations, are mostly located on the slope of mountains facing north at an altitude of 343 m to 991 m above sea level with angle of inclination from 1 degree to 33 degrees. The type of soil is mostly sandy loam and the average field capacity of soil is 28.97%. Their average organic matter is 16.63%, soil pH 5.62, and available phosphorus is 14.75%. A total of 133 vascular plants are identified in 18 quadrates of seven habitats. Dominant species of woody plants in seven habitats are represented as Acer pictum subsp. mono and Quercus mongolica in tree layer, and Acer pictum subsp. mono in subtree layer. Importance value of Viola websteri is 9.66%, as regards the herbaceous layer, and five highly ranked species such as Meehania urticifolia(8.53%), Pseudostellaria heterophylla(6.51%), Hylomecon vernalis(5.15%), Oxalis obtriangulata (4.52%), and Pseudostellaria davidii(4.15%) are considered to be an affinity with Viola websteri in their habitats. The degree of their average species diversity is 1.32, and that of dominance and evenness are 0.08 and 0.89, respectively. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of Viola websteri is correlated with silt and sand ratio, and coverage of tree layers are correlated with species richness and altitude. Cluster analysis based on vegetation structure of each habitats are forms a three groups.

The Weed Flora of Korean Mulberry Fields (뽕밭에서 발생하는 잡초 양상)

  • Lee, In-Yong;Kim, Chang-Seok;Lee, Jeongran;Song, Hee-Kun;Seo, Hyun-A;Choi, Kyung-Mi;Ji, Sang-Deok
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.85-94
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    • 2015
  • The weed flora in the mulberry fields were investigated in Suwon, Jeonju, and Buan in May, July, and September of 2014. The objectives of this study were to use the survey data for establishing weed control methods and to bring awareness of possible problematic weeds in the Korean mulberry fields. The survey was conducted in 53 regions, covering approximately $145,925m^2$. Altogether 153 weed species of 37 families were identified, of which 68 were annual, 39 species were biennial and 46 were perennial. The dominance was the highest with Digitaria ciliaris followed by Erigeron annuus, Chenopodium album, Echinochloa crus-galli var.crus-galli, Acalypha australis, Commelina communis etc. Exotic weeds presented 44 species with 28.8% of a total presence, of which Erigeron annuus was the highest, followed by Chenopodium album, Phytolacca americana, Conyza canadensis, Oxalis corymbosa etc. Especially, we should aware Senecio vulgaris, not controlled with glufosinate ammonium SL in the Korean mulberry fields because it was known as atrazine resistance in US, Canada, Germany etc. In the PCA plot, weeds presented in the mulberry fields were divided into two groups, Eclipta prostrata community and Stellaria aquatic community and weed flora of Suwon and Buan were different due to those only presented in Suwon.

Occurrence of Weed Species on Turf Sod Production Areas in Jangsung-gun, Jeonnam Province (전남 장성지역 한국잔디 재배지 잡초발생 현황)

  • Choi, Sung Hwan;Ahn, Soo Jeong;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.247-255
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    • 2016
  • This study was conducted to provide basic information for weed control by surveying the occurrence of weed species in turf sod production areas. Surveys of weed species occurred in turf sod production areas were conducted in Jangsung-gun, Jeonnam province from September 2014 to May 2016. Total 50 sites of turf sod production areas in two soil conditions were investigated. On the upland soil condition in turf sod production areas, 66 weed species in 27 families were identified and classified to 49 annuals and 17 perennials and on the paddy soil condition, 69 weed species in 22 families were identified and classified to 53 annuals and 16 perennials. Based on the importance values, the most dominant weed species on the upland soil condition in the first survey (September 2014) was Digitaria ciliaris (8.49%), followed by Erigeron annuus (7.94%) and Rorippa indica (6.56%). In the second survey (May 2016) was Oxalis corniculata (7.26%), followed by Capsella bursa-pastoris (6.21%) and Conyza canadensis (6.21%). Whereas the most dominant weed species on the paddy soil condition in the first survey (September 2014) was Erigeron annuus (9.52%), followed by Mazus pumilus (7.41%) and Cyperus iria (6.82%). In the second survey (May 2016) was Commelina communis (5.08%), followed by Alopecurus aequalis (5.08%) and Erigeron annuus (4.79%). This information could be useful for estimation of future weed occurrence and effective weed control methods in turf sod production areas in Jangsung-gun, Jeonnam Province.

The Flora of Three Eastern Mountains(Susdolbong(Mt.), Seongjubong(Mt.), Sulibong(Mt.)) of Paroho Lake in Yanggu-gun, Korea (강원도 양구군 파로호 동부 3개 산지(숫돌봉, 성주봉, 수리봉)의 관속식물상)

  • Song, Jin-Heon;Shin, Hyun-Tak;Yun, Ho-Geun;Kim, Sang-Jun
    • Korean Journal of Environment and Ecology
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    • v.35 no.1
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    • pp.1-23
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    • 2021
  • This study conducted a survey on flora at Mt. susdolbon(508m), Mt. seongjubong (625.3m), and Mt. sulibong(596.2m), located in Yanggu-gun, Gangwon-do. A total of 403 taxa, including 6 forms, 43 varieties, 4 subspecies, 350 species, 255 genera, and 84 families, were surveyed in the three mountain areas. 18 taxa from 5 families of ferns, 3 taxa from 1 family of gymnosperms, and the dicotyledonous plants as 302 taxa from 69 families of the dicotyledonous plants were identified. The remarkable plants included 11 taxa of the Korean endemic plants, such as Populus tomentiglandulosa, Salix koriyanagi, Cimicifuga heracleifolia, Aconitum pseudolaeve, Clematis trichotoma, Echinosophora koreensis, Vicia chosenensis, Salvia chanryoenica, and Hemerocallis hakuunensis. The rare plants designated by the Korea Forest Service were 4 taxa, including Echinosophora koreensis as endangered species (EC), Cimicifuga heracleifolia, Epimedium koreanum, and Lilium cernuum. The Least Concern species (LC) were 13 taxa, including Persicaria orientalis, Exochorda serratifolia, Viola diamantiaca, Lithospermum erythrorhizon, and Trigonotis icumae. The invasive alien plants were 28 taxa, including Persicaria orientalis, Rumex crispus, Phytolacca americana, Cerastium glomeratum, Chenopodium album, Chenopodium ficifolium, Amorpha fruticosa, Robinia pseudoacacia, Trifolium pratense, Trifolium repens, Oxalis dillenii, Euphorbia supina, and Oenothera biennis, accounting for 6.9 percent of the 403 taxa of the surveyed plants.

Weed Flora of Ulreung Island in Korea (울릉도(鬱陵島)에 발생(發生)하는 잡초종(雜草種))

  • Kim, K.U.;Kwon, S.T.;Lee, I.J.;Kim, S.H.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.265-279
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    • 1987
  • About 140 weed species belonging to 42 families occurring in summer were observed in Ulreung island. The Compositae was the most wildely occurring family covering 27 weed species, followed by 21 species in Graminae, 9 in Polygonaceae, 7 in Leguminosae, and 7 in Labiatae etc. In terms of the lands classified, about 60 species in 26 families were observed in the cultivated and the medical crops grown areas, respectively, and 116 species in 40 families occurred in the non-cultivated land like the vicinity of the cultivated area and 94 species in 34 families in the valley. No. of species and families were much greater in the non-cutivated land than those of the cultivated one. The most dominant weed species in both the cultivated and its vicinity in Ulreung island were Digitaria sanguinalis, followed by Portulaca oleracea, Polygonum hydropiper, Equisetum arvensis, Artemisia princeps, Commetina communis, Setaria viridis in order. Community analysis was done by the method of Toyohara in two cultivated lands such as the general crop land and the medical herb crop grown land, and two non-cultivated areas such as the vicinity of the cultivated land and valley. The cultivated land consisted of the communites of P. hydropiper and Cyperus amuricus, including Bidens tripartita in P. hydropiper community. The medical crop grown land composed of three major communities like P. hydropiper, Amaranthus mangostanus and Sonchus asper in which A. mangostanus and S. media were presented in the ecoton, indicating community being transiting. In the non-cultivated areas like the vicinity of crop land, the communities of Erigeron annuus, P. oleracea, and Oxalis corniculata were dominant, and Dystaenia takeshimana was included in the community of E. annuus and Stellan'a aquatica in P. oleracea community. In the valley, Ranunculus quelpaertensis community was existed in between the communities of Sonchus asper and Plantago asiatica.

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Floristic features of upland fields in South Korea (우리나라 밭 경작지에 출현하는 식물상 특성)

  • Kim, Myung-Hyun;Eo, Jinu;Kim, Min-Kyeong;Oh, Young-Ju
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.528-553
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    • 2020
  • Upland fields are characterized by dry environments, a high degree of disturbance by farming practices such as double-cropping, and a high diversity of crops compared to other field types. This study focused on the floristic composition and characteristics of upland fields in South Korea. Flora surveys were conducted in 36 areas in nine provinces at two times (June and August) in 2015. The results showed that the vascular plants in the upland fields in South Korea included 532 taxa, containing 100 families, 322 genera, 483 species, nine subspecies, 37 varieties, one form, and two hybrids. Among the 100 families, Asteraceae was the most diverse in species (75 taxa), followed by Poaceae (68 taxa), Fabaceae (34 taxa), Polygonaceae (21 taxa), Rosaceae (19 taxa), and Liliaceae (17 taxa). Based on the occurrence frequency of each species, Acalypha australis L. (100%), and Artemisia indica Willd. (100%) were the highest, followed by Humulus scandens (Lour.) Merr., Rorippa palustris (L.) Besser, Conyza canadensis (L.) Cronquist, Erigeron annuus (L.) Pers., Lactuca indica L., Commelina communis L., Digitaria ciliaris (Retz.) Koeler, Echinochloa crus-galli(L.) P.Beauv., Cyperus microiria Steud., and Oxalis corniculata L. The biological type of upland fields in South Korea was determined to be Th-R5-D4-e type. Rare plants were found in 11 taxa: Taxus cuspidata Siebold & Zucc, Magnolia kobus DC, Clematis trichotoma Nakai, Aristolochina contorta Bunge, Buxus sinica (Rehder & E.H.Wilson) M.Cheng var. koreana (Nakai ex Rehder) Q.L.Wang, Melothria japonica (Thunb.) Maxim, Mitrasacme indica Wight, Lithospermum arvense L., Carpesium rosulatum Miq., Allium senescens L., and Pseudoraphis sordida (Thwaites) S.M.Phillips & S.L.Chen. Ninety-seven taxa contained naturalized plants composed of 24 families, 68 genera, 97 species, one variety, and one form. The urbanization and naturalization indices were 30.5% and 18.4%, respectively.