• 한국임상수의학회지
• /
• 제16권2호
• /
• pp.369-374
• /
• 1999

One hundred forty cows that have claw disorder have been used to study the association between lameness and reproductive disorders. Weak estrus owing to ovarian hypoplasia (41.4%) followed by follicular cyst (39.3%) was more frequent than any other disorders. Two hundred ninety-four cows have been used to study the association between lameness and calving to first service and calving to conception intervals depending on lactations, claw disease and floor conditions. And a comparison of number of services per conception between cows with lameness and cows free from lameness was carried out. The calving to first service intervals were significantly increased from average 78 to 102.5 days and calving to conception intervals were significantly increased from 109.6 to 150.6 days in cows with lameness. The calving to first service and calving to conception intervals were not significant different between lactations in cows with lameness. In comparison of calving to first service and calving to conception intervals in cows with lameness depending on claw disease and floor conditions, there was no significant difference. The average number of services per conception was increased from 1.73 to 2.57 in cows with lameness. Service numbers were more frequent during second lactation (3.0 services) but there was no significant difference between lactations in cows with lameness. Therefore, it is likely that the management of lameness is important factor on proper health control of cows and the cows with lameness show decreased fertility.

• 한국발생생물학회지:발생과생식
• /
• 제25권3호
• /
• pp.123-131
• /
• 2021

We report on the gonadal structure abnormality and intersexuality found from a survey regarding the reproductive potential of the rock bream, Oplegnathus fasciatus, along the southern coast of Korea. In total, 448 samples were collected for histological analysis (total length 27.5±6.8 cm; total weight 522.9±385.6 g). The sex ratio (F:M) was 1:0.46 (n=307:141), with 68.5% being females. The frequency of oocyte atresia and ovarian neoplasm in the females was 85.3% and 21.5%, respectively, and the frequency of testicular lobule and cyst deformation and testicular neoplasm in the males was 73.1% and 37.6%, respectively. The scale formation in the ovary and testis was approximately 2.0% for both sexes. The intersexuality was 42.2%, with the intersexuality among females being 49.5%, which was significantly higher than that of males (26.2%). Such findings indicate a negative impact on the reproductive output of the rock bream along the southern coast of Korea; however, a more detailed study is required to ascertain accurate causes.

• Reproductive and Developmental Biology
• /
• 제29권1호
• /
• pp.37-41
• /
• 2005

본 연구는 한우 수정란의 체외생산에 있어서 효율과 품질의 향상을 위해서, 미성숙 난포란을 회수하는 난소의 형태와 배양 용기로서 straw의 효과를 검토하였다. 난소의 형태에 따른 수정율은 전 군에서 70.3∼84.1%로서 비슷한 경향이었다. 8세포기 및 배반포기 발달율은 황체와 난포가 모두 존재하지 않는 대조군이 가장 높았다. 배반포의 inner cell mass(ICM), trophectoderm(TE), total cell number(TCN) 및 ICM/TCN 비율은 낭종군과 퇴행황체군이 다른군에 비하여 높은 경향이었다. 체외성숙에 이용하는 배양용기에 따른 수정율은 0.5 ㎖ straw 군이, 8세포기 발달율은 대조군이 가장 높았으나, 배반포기 발달율은 23.1∼30.7％로서 각 군 간에 비슷한 경향이었다. 한편 각각의 배양 용기에서 유래된 배반포의 ICM, TE, TCN 및 ICM/TCN 비율은 유사한 경향이었다.

• 한국수정란이식학회지
• /
• 제26권1호
• /
• pp.53-56
• /
• 2011

Infertility of the pig is directly affects on economic loss and failure of the embryo transfer. In the present case report, we show one rare case of porcine infertility resulted from oviductal obstruction. A gilt showing normal heat behaviors was selected as a recipient of embryo transfer. During the laparotomy surgery, abnormality of the reproductive tract was founded. Several large sized cyst-like structures were founded on infundibulum and body of uterus. Severe enlargement of oviduct represents that obstruction of the oviduct. Sign of fibrosis on the surface of uterus and other internal organs revealed that the obstruction was come arise from prior peritonitis. Mild neutropenia and elevated number of monocytes, eosinophils and platelets in blood smear represent that the peritonitis might be due to chronic parasitic infection. Ovarian function was seems to be normal due to blood progesterone concentration was higher than basal level. The pig was culled because she cannot be recovered by surgical or hormonal treatment.

• Environmental Analysis Health and Toxicology
• /
• 제22권1호
• /
• pp.83-90
• /
• 2007

This study was investigated the effort of growth and gonadal development in olive flounder, Paralichthys olivaceus exposed to PCBs Effluent Allowance Concentration, 3.0 ppb in Korea. Total length, body weight and weight gam of fish were no significant differences between two treatments (control: T.L.-110.0 mm, T.W.-9.61 g: treatment: T.L.-112.8 mm, T.W.-9.68 g), however, weight gain of fish unexposed to PCBs wat observed to increase gradually from 100 days after hatching (30 days after treatment). In the indifferentiated gonad to the juvenile stage, the differentiation of the ovary occured at 60 days after hatching (20 days after treatment) and that of the testis occured at 70 days after hatching (30 days after treatment) in two groups. In the ovary, the oogonia and ovarian lamellar appeared 130 days after hatching (50 days after treatment). The spermatogonia and resticular lobule of the testis were observed at 130 days after hatching (50 days after treatment). Testicular cyst appeared 140 days after hatching (60 days After treatment). There was no difference on the development of ovary and testis. There was no obvious difference of sex ratios. Therefore, These results considerate that PCBs have any effect for growth and gonadal development, but a little act for early growth in olive flounder.

• 한국수정란이식학회지
• /
• 제29권2호
• /
• pp.189-194
• /
• 2014

The reproductive disorders are the major causes of reproductive infertility in cows that affect the total annual calf crop, resulting in great economic loss in Bangladesh. The aim of the study was to find out the reproductive disorders (RD) in dairy cows that markedly influences the reproductive performances in aspect of Bangladesh. A total number of 1658 dairy cows were selected according to their body condition score (BCS) in different farms at the southern part of Bangladesh during the period of 2011 to 2012. The preliminary data (basic information) were collected directly from the dairy farmer's record books and asking questions according to a prescribed questionnaires as well as the diagnosis of RD was presumptively confirmed on the basis of history, clinical signs and examination of animals by ultrasonography and others necessary tools. There are thirteen major reproductive disorders were identified. Overall prevalence of reproductive disorders at that area were 23%, among of these anoestrus 5.1%, repeat breeder 3.7%, metritis 4.4%, poor heat detection 1.6%, ovarian cyst 0.36%, retain placenta 4.6%, dystocia 0.97% and pyometra 0.24%. It is indicated that anoestrus and retention of placenta after calving was most hazardous cause of infertility whereas the metritis and repeat breeder were the second line of consequence. RD had shown significantly higher incidence in low BCS (${\leq}2$) than that of fair (2.5) and very good (${\geq}3{\sim}3.5$). In conclusion, the highest RD especially anoestrus and retention of placenta is very alarming for reproductive loss which might be needed further research to identify the specific cause of these disorders for establishment a profitable dairying and dairy population.

• 한국임상수의학회지
• /
• 제20권2호
• /
• pp.159-165
• /
• 2003

This study was performed to determine the effect of green tea on the late biological effect (survival, hematological change, carcinogenesis) of mice irradiated with 3 Gy of gamma-radiation. There was little difference in body weights between normal and irradiated mice. Survival rate were decreased in irradiated mice and the survival rate and mean survival time of the groups treated with green tea were far better than the irradiation control group. An elevation of mean number of total leukocyte or lymphocyte counts was seen at week 12 of the group treated with green tea. Stimulated recovery by the extract from green tea was also observed in thrombocyte. Main gross findings of irradiated mice were appeared as enlargement of spleen, thymus and liver, tumorous nodules in lung and cyst or mass in ovary. Microscopically, there were various findings including hematopoietic and lymphoid tumor, lung cancer, ovarian cancer and cancer of other lesions. Green tea reduced the incidence of tumor development. Further studies are needed to characterize better the protective nature of active compounds.

• The Korean Journal of Physiology and Pharmacology
• /
• 제14권5호
• /
• pp.265-272
• /
• 2010

Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and $\geq25mm$). These follicles had granulosa cell layer and theca interna and the hormone $17{\beta}$-estradiol ($E_2$)/ progesterone ($P_4$) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using $GeneFishing^{TM}$ DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

• 한국임상수의학회지
• /
• 제30권4호
• /
• pp.310-314
• /
• 2013

12년령 암컷 혼혈견이 외음부에 돌출된 질 종양으로 내원하였다. 이 환자는 정상적으로 변과 오줌을 배출하였고 임신 경험은 없었다. 혈청생화학적인 소견상 질소혈증, 경미한 고나트륨 혈증과 alkaline phosphatase 값의 상승이 있었다. 혈액학적 검사 소견은 정상이었다. 초음파 검사에서 좌 우측 자궁각의 내강에 존재하는 액체로 인하여 무에코성에서 저에코성으로 나타났고 심하게 종대되어 있었다. 또한, 우측 난관의 종양 내에 국소적으로 저에코성 영역이 나타났다. 컴퓨터단층촬영에서는 우측 난소낭, 액체로 인해 확장된 자궁, 확장된 질과 질의 종양이 관찰되었다. 본 환자에 대해 난소자궁적출술과 회음부 절개술을 통한 종양절제술을 실시하여 난관과 질에 존재하는 종양을 제거하였다. 본 환자는 난관 선암종, 질 섬유종, 자궁축농증이 병발한 첫 번째 사례로 여겨진다.

• 대한수의학회지
• /
• 제15권2호
• /
• pp.181-185
• /
• 1975

The experiment was carried out to determine the concentrations of total calcium and inorganic phosphorus of thirty rera obtained from pregnant Holitein cows, six sera from normal non-pregnant cows with one month after delivery, six sera from cows with ovarian cyst and six sera from heifer of sixteen months old. The changes of total calcium and inorganic phosphorus were observed on the 2nd, 4th, 6th, 8th, and l0th month of gestation. The results obtained in this work were summarized as follows: 1. The mean values of total serum calcium obtained from the pregnant group were $9.59{\pm}0.34mg/100ml$, from the non-pregnant group $10.23{\pm}0.47mg/100ml$, from the infertility grou $10.37{\pm}0.27mg/100ml$, and from the heifer group $10.07{\pm}0.20mg/ml$. The change of total serum calcium values in the pregnant group revealed the tendency of decrease as gestation progresses and the lowest value appeared on the sixth month. The mean value of total serum calcium in the pregnant group was lower than those of the other groups, but the differences were not statistically significant. 2. The mean values of serum inorganic phosphorus obtained from the pregnant group were $5.82{\pm}0.31mg/100ml$, from the non-pregnant group $5.92{\pm}0.44mg/100ml$, from the infertility group $5.80{\pm}0.49mg/100ml$, and from the heifer group $5.10{\pm}0.32mg/100ml$. The change during pregnancy showed a slight fall and the lowest value appeared on the sixth month. The differences of serum inorganic phosphorus values between the pregnant group and the other adult groups were not significant, but these adult groups showed a significantly higher value than that of heifer group (p<0.05).