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Effects of the Morphology of Ovaries and Vessels for In Vitro Maturation on the Development and Cell Number of Korean Native Cow Embryos  

Park Y. S. (Division of Food and Biotechnology, Daegu University)
Park H. D. (Division of Food and Biotechnology, Daegu University)
Publication Information
Reproductive and Developmental Biology / v.29, no.1, 2005 , pp. 37-41 More about this Journal
Abstract
The aim of this study was to improve efficiency and quality of the production of Korean Native Cow embryos. We examined effects of ovarian morphology and maturation vessel on the development and cell number of blastocysts. The development rates to the 2-cell embryos from oocytes collected from the ovaries of different morphological statues were similar ranging between 70.3 and 84.1%. The development rate to the 8 cell- and blastocyst-stage embryos was the highest in the group without both corpus luteum (CL) and follicle. The inner cell mass (ICM), trophectoderm (TE) and total cell number (TCN) were significantly higher in the groups of follicular cyst and regressive CL than other treatment groups, and the same pattern was observed in the ICM/TCN ratio. The development rate to the 2-cell stage was significantly higher in 0.5-㎖ straw group than 0.25-㎖ straw group. However, the development rates to the blastocyst stage were similar between the dish and the straw group. There were no differences in the number of ICM and TE cells, TCN and ICM/TCN ratio of blastocysts from oocytes matured in the different vessels.
Keywords
In vitro maturation; Ovary; Straw; Embryo development; Cell number;
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