• 동의생리병리학회지
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• 제16권4호
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• pp.788-800
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• 2002

Aging is characterized by an age-dependent reduction in bone density. Pronounced bone loss by postmenopausal estrogen deficiency induces a bad effect on dynamic balance of bone metabolism. When the bone density is reduced to the level below the fracture threshold, the risk for fracture is greatly increased. The purpose of this study is to examine what are the effects of the Yuklinzu(YLZ: 毓麟珠) on the ovariectomized rat model of postmenopausal osteoporosis. The results of the experiment are as follows: Body weight in control group showed significant increase in comparison with sham, but that in YLZ-treated showed no change in comparison with control. The level of serum albumin in control group showed significant decrease in comparison with sham. That in YLZ-treated was slightly decreased in comparison with control. The level of serum ALP activity in control group showed significant increase in comparison with sham, but that in YLZ-treated was significantly decreased in comparison with control. The level of serum phosphorous in control group showed significant increase in comparison with sham, but that in YLZ-treated showed no change in comparison with control. The level of serum calcium in control group showed no change in comparison with sham, but that in YLZ-treated was significantly decreased in comparison with control. The level of serum GOT in control group showed no change in comparison with sham, but that in YLZ-treated was significantly decreased in comparison with control. The level of serum GPT did not significant change among the three groups. The level of serum estrogen in control group showed slightly increased in comparison with sham, but that in YLZ-treated showed no change in comparison with control. The level of serum T3 in control group showed no change in comparison with sham, and that in YLZ-treated showed no change in comparison with control. The level of serum T4 in control group showed significant increase in comparison with sham, but that in YLZ-treated was significantly decreased in comparison with control. Mechanical strength of the femur neck in control group showed significant decrease in comparison with sham. That in YLZ-treated showed slightly increased in comparison with control. Trabecular bone area as well as trabecular thickness in control group showed significant decrease in comparison with sham. Those in YLZ-treated showed significant increase in comparison with control. Trabecular number and did not significant change among the three groups. Trabecular separation only in YLZ-treated showed significant decrease in comparison with control. Osteoclast number and oseoblast surface in control group showed significant increase in comparison with sham. Those in YLZ-treated showed slightly decreased in comparison with control. According to the above these results, YLZ has shown to be capable of preventing and curing osteoporosis caused by old-aged and postmenopause.

• 한국식품영양과학회지
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• 제43권1호
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• pp.24-29
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• 2014

본 연구는 구기자와 구기엽 추출물이 난소를 절제한 흰쥐의 체중 증가, 골밀도, 에스트로겐 수용체의 발현에 미치는 영향을 밝히고자 시행하였다. 실험군은 흰쥐 24마리를 가장수술군(Sham군), 난소를 절제한 대조군(OVX군), 난소절제 후 구기자 추출액을 투여한 군(LCF군), 난소절제 후 구기엽 추출액을 투여한 군(LCL군)으로 구분하였다. 8주 후 난소를 절제한 OVX군($330{\pm}5.39g$), LCF군($315{\pm}2.99g$) 및 LCL군($318{\pm}2.06g$)의 체중은 Sham군($281{\pm}1.71g$)에 비하여 증가하였다. 혈청 osteocalcin 활성은 OVX군($107{\pm}3.52ng/mL$)에 비하여 LCF군($444.6{\pm}26.9ng/mL$)과 LCL군($407{\pm}18.9ng/mL$)에서 증가하였다. Alkaline phosphatase의 활성은 OVX군($95{\pm}2.9U/L$)에 비하여 LCF군($108{\pm}2.7U/L$)과 LCL군($407{\pm}18.9ng/mL$)에서 증가하였다. 8주 후 넙다리뼈를 실체현미경으로 관찰한 결과 뼈 기질밀도는 난소절제군에서 감소하였으나, 구기자와 구기엽을 추출액을 투여한 군에서는 뚜렷이 회복되었다. 에스트로겐 수용체의 mRNA 발현은 OVX군에서는 거의 발현되지 않았으나, LCF군과 LCL군에서 뚜렷이 증가하였다. 이상의 결과로 보아 구기자와 구기엽 물추출물이 난소절제에 의하여 유발된 흰쥐의 뼈 기질 회복 또는 손실 지연에 효과가 있다고 할 수 있을 것이다.

• 약학회지
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• 제43권1호
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• pp.111-116
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• 1999

Obesity is a chronic disease that is increasing in prevalence and that poses a serious risk for the hypertension, osteoporosis, heart disease, diabetes mellitus and certains forms of cancer. This study was performed to develop of obesity animal model and to assess the pharmacological assay for the rats of 8 weeks or 4 days after ovariectomization treated with estradiol for 8 weeks on the body weight. fat weight and food intake. The body weight, fat weight and food intake increased in the ovriectomized rats. In the rat of 8 weeks after ovariectomization treated with estradiol (250 mg/100 g) 8 weeks, the body weight decreased significantly (p<0.05). In the rats of 4 days after ovariectomization treated with estradiol 8 weeks, the body weight decreased significantly (p<0.05). These results suggest that estrogen plays a role in regulation body weight response to food intake and fat weight.

• Journal of Korean Neurosurgical Society
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• 제54권6호
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• pp.461-466
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• 2013

Objective : Although curcumin has a protective effect on bone remodeling, appropriate therapeutic concentrations of curcumin are not well known as therapeutic drugs for osteoporosis. The purpose of this study was to compare the bone sparing effect of treatment of low-dose and high-dose curcumin after ovariectomy in rats. Methods : Forty female Sprague-Dawley rats underwent either a sham operation (the sham group) or bilateral ovariectomy (OVX). The ovariectomized animals were randomly distributed among three groups; untreated OVX group, low-dose (10 mg/kg) curcumin administered group, and high-dose (50 mg/kg) curcumin group. At 4 and 8 weeks after surgery, serum biochemical markers of bone turnover were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae were determined by micro-computed tomography (CT). In addition, mechanical strength was determined by a three-point bending test. Results : High-dose curcumin group showed significantly lower osteocalcin, alkaline phosphatase, and the telopeptide fragment of type I collagen C-terminus concentration at 4 and 8 weeks compared with the untreated OVX group as well as low-dose curcumin group. In the analyses of micro-CT scans of 4th lumbar vertebrae, the high-dose curcumin treated group showed a significant increase in bone mineral densities (p=0.028) and cortical bone mineral densities (p=0.036) compared with the low-dose curcumin treated group. Only high-dose curcumin treated group had a significant increase of mechanical strength compared with the untreated OVX group (p=0.015). Conclusion : The present study results demonstrat that a high-dose curcumin has therapeutic advantages over a low-dose curcumin of an antiresorptive effect on bone remodeling and improving bone mechanical strength.

• Applied Microscopy
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• 제32권3호
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• pp.285-290
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• 2002

홍화씨는 한방 및 민간에서 뼈에 우수한 작용이 있는 것으로 알려져 오랫동안 복용되어 왔다. 최근 고령화 사회를 맞이하면서 골다공증 문제는 사회적인 문제로 인식되고 있으며 저자들이 기존에 실시한 실험결과에 의하면 홍화씨 분말은 난소를 적출한 rat에서 골다공증의 예방효과를 나타내었다. 그러나 아직까지 골다공증이 유발된 rat에서 홍화씨 분말이 미치는 효과에 관한 실험적 자료가 제시된 것은 별로 없다. 이에 저자들은 홍화씨 분말이 골다공증의 치료에 미치는 효과를 알아보기 위하여 본 실험을 실시하였다. 실험동물은 체중 230 g의 12주령의 Sprague-Dawley Rats를 사용하였으며, 양쪽 난소제거 후 7주부터 홍화씨 분말을 매일 0.3 g씩 복용시키면서 1, 3 및 5주 후에 경골을 채취하여 관찰하였다. 채취된 조직은 통상적인 주사전자현미경 시료제작법으로 고겅과정을 거친 후 10% 질산으로 12시간 탈회하여 뼈의 단면을 노출시키고 탈수, 건조 및 금도금 과정을 거쳐 주사전자현미경(Hitachi, S-450)으로 관찰하여 촬영하였다. 관찰결과 대조군은 골수강에서 피질골까지의 두께의 감소와 골수강의 확장이 심하였으나, 홍화씨 분말을 투여한 실험군에서는 1주에서 5주까지 거의 같은 소견을 나타내었다. 이상의 결과를 종합해보면 홍화씨 분말은 여성호르몬 결핍으로 인한 골다공증의 치료에 효과가 있는 것으로 사료된다.

• 대한한방부인과학회지
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• 제29권2호
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• pp.1-14
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• 2016

Objectives : In this study, the author aimed to evaluate the effects of water extract of Dokwhalgisaeng-tang Gamibang (DGG) on osteoblast proliferation in murine calvarial cells. Methods : The osteoblast separated from murine calvariae was cultivated and evaluated the cell function. After the addition of DGG on the culture medium, we determined the effect of DGG on the cell proliferation, protein synthesis, alkaline phosphatase activity, collagen synthesis, and cell viability of the cultivated osteoblast in the presence of dexamethasone. Results : DGG increased the survival rate, proliferation, alkaline phosphatase (ALP) activity, protein synthesis and collagen synthesis of rat calvarial osteoblast in the presence of dexamethasone. Conclusions : DGG might improve the osteoporosis resulted from augmentation of osteoblast proliferation.

• 대한의생명과학회지
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• 제16권4호
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• pp.213-220
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• 2010

Although an adequete intake of calcium (Ca) is recommended for the treatment and prevention of osteoporosis, the intake of Ca should be restricted because of its low rate of intestinal absorption. The purpose of this experiment was to identity the effect of the combined administration of Aquamin F (AQF) (a calcium agent) and lactic acid bacteria (LAB) on osteoporosis. Thirty ovariectomized (OVX) rats and six control rats were assigned to the following six groups, with six animals per group: sham Ca-deficient diet (Ca-D), OVX, LAB, AQF, and LAB-AQF. During the experiment, the body weight was measured; and after the experiment was completed, the serum biochemical analysis, the alkaline phosphatase, calcium, and inorganic phosphorus leves were measured. The tissue of the femur was stained and then scanned via CT. The body weight of the OVX group increased more significantly than that of the control group. The results of the bone mineral content (BMC), Bone mineral density (BMD), serum biochemical analysis and histological test on the femur epiphysis showed no difference between the OVX group and the LAB group, whereas the results of the AQF group were more significant than those of the OVX group. In particular, the LAB+AQF group showed more significant increases in the aforementioned results than the AQF group. This experiment showed that the combined administration of AQF and LAB in ovariectomized rats more significantly increased bone density than did a single administration of either AQF or LAB.

• 대한수의학회지
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• 제40권4호
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• pp.755-762
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• 2000

The aim of this investigation was to examine the effects of osteocalcin, bone-specific alkaline phophatase, estrogen, insulin-like growth factor-I (IGF-I), Ca, P and bone mineral density on osteoporosis induced by ovariectomy in rats. Female Sprague-Dawley 30 rats of three-forth's birth, weighing $215{\pm}10g$, were divided into two groups including the sham operation group(5 heads) and ovariectomy group(25 heads). They were fed normal diets for 2 weeks before the experimental operation and for 8 more weeks after operation. The level of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were increased in experimental group, but a little increased in sham operation group at same period. The change of rates of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were significantly higher in experimental group than sham operation group. $Ca^{2+}$ was not changed between two groups and P was significantly decreased in experimental group and Ca/P ratio was higher in experimental group than sham operation group. Body weights were increased in all two groups and growth rate per day was higher in experimental group than sham operation group. However, femur weight I body weight ratio was lower in experimental group than sham operation group.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.188-205
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• 2001

Objective : The purpose of this studies was to determine the effect of Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. on changes of trabecular area and physiological metabolites in the ovariectomized osteoporotic Rats. Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then the Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. were orally administerd: Such indexes were measured as the changes of body weight, bone mineral density, trabecular area in tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus, cholesterol, triglyceride, aspartate aminotransferase and alanine aminotransferase in serum. Results : 1. The change of bone mineral density in Bogol-Tang group and Rhynchosia volubilis lour. group. was significantly increased compare to control group. 2. The change of trabecular area % in epiphysis of tibia in Carthami semen group was significantly increased compare to control group. 3. The change of trabecular area % in diaphysis of tibia in Bogol-Tang group was significantly increased compare to control group. 4. The change of serum osteocalcin in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 6. The change of1 serum calcium in Rhynchosia volubilis lour. group was significantly decreased compare to control group. 7. The change of phosphorus in Carthami semen group and Rhynchosia volubilis Lour. group was significantly decreased compare to control group. 8. The change of serum cholesterol and triglyceride of experimental groups were decreased in comparison with control group. 9. The change of serum AST(Aspartate aminotransferase : GOT) in Rhynchosia volubilis Lour. group was decreased compare to control group. 10. The change of serum ALT(Alanine aminotransferase : GPT) in Bogol-Tang group was decreased compare to control group.

• 동의생리병리학회지
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• 제21권4호
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• pp.967-972
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• 2007

Osteoporosis is the most prevalent metabolic bone disease and is characterized by diminished bone strength predisposing to an increased risk of fracture. We investigated the effects of the extract from Acyranthes Radix on the progress of bone loss in ovariectomized rats for 6 weeks. Female Sprague-Dawley 40 rats of 3-4 weeks, weight 200 ${\pm}$ 10g were divided into two groups including the sham operation group(8 rats) and ovariectomy group(32 rats). The dose-dependent effect of Acyranthes Radix extract on bone mineral density and biochemical testing was assessed in ovariectomized rat. Body weights were increased in all groups was higher in experimental group than sham operation group. The level of bone mineral density, GPT, and serum P concentration, ALP were increased experimental group, but a little increase in sham operation group at same period. This longitudinal study result made conclusion that Acyranthes Radix extract treatment appeared to improve the osteoporosis delaying the progression to the osteoporotic process in rats.