• 대한물리의학회지
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• 제8권1호
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• pp.127-135
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• 2013

PURPOSE: This study examined the effects of exercise of diverse intensities on the recovery of articular cartilage in osteoarthritic rats. METHODS: Over a period of four weeks, the authors applied treadmill exercise programs of diverse intensities to Sprague-Dawley rats, to which intra-articular injection of monosodium iodoacetate(MIA, 3mg/$50{\mu}l$, diluted in saline) was applied to the right knee joint to induce osteoarthritis. The four-week exercise program was not carried out with the control group(CG, n=10). Exercise programs of applicable intensities were applied to the low-intensity exercise group(LEG, n=10), moderate-intensity exercise group(MEG, n=10), and high-intensity exercise group(HEG, n=10) over the four weeks. Observations were made of morphological changes in the rats' articular cartilage, using hematoxylin and eosin stains. RESULTS: there were significant differences(p<.05) in the comparison of articular damage scores between the four groups involved. Articular cartilage damage scores were found to be significantly lower in the LEG, MEG, and HEG than in the CG, indicating that exercise helped with the recovery of cartilage. Of these latter three groups, the MEG showed the highest level of recovery, while the HEG showed the lowest. CONCLUSION: These study results suggest that exercise is effective in treating OA. They also indicate that in prescribing exercise to treat osteoarthritic patients, exercise of moderate intensity is most suitable to patients' physical conditions, rather than low or high intensity, maximizes, and so should be used to maximize the effects of therapy.

• 대한물리의학회지
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• 제8권4호
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• pp.539-547
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• 2013

PURPOSE: This study examined the effects of exercise of diverse intensities on the expression of TNF-${\alpha}$(tumor necrosis factor-${\alpha}$) in the spinal cord in osteoarthritic rats. METHODS: Over a period of four weeks, the authors applied treadmill exercise programs of diverse intensities to Sprague-Dawley rats, to which intra-articular injection of monosodium iodoacetate(MIA, $3mg/50{\mu}l$, diluted in saline) was applied to the right knee joint to induce osteoarthritis. The four-week exercise program was not carried out with the control group(CG, n=10). Exercise programs of applicable intensities were applied to the low-intensity exercise group(LEG, n=10), moderate-intensity exercise group (MEG, n=10), and high-intensity exercise group(HEG, n=10) over the four weeks. Observations were made of expression of TNF-${\alpha}$ in the spinal cord in osteoarthritic rats using western blot analysis. RESULT: there were significant differences(p<.05) in the comparison of expression of TNF-${\alpha}$ between the four groups involved. The expression of TNF-${\alpha}$ in the spinal cord, the LEG and HEG had more elevated expression significantly than the CG(p<.05). But the MEG had reduced expression significantly than the CG(p<.05). CONCLUSION: These study results suggest that moderate-intensity exercise is effective in inhibition TNF-${\alpha}$ in the spinal cord. They also indicate that in prescribing exercise to treat osteoarthritic patients, exercise of moderate intensity is most suitable to patients' chronic pain, rather than low or high intensity.

• 대한한의학회지
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• 제28권4호
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• pp.114-123
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• 2007

Osteoarthritis is characterized by cartilage degradation and chondrocytes death. Chondrocyte death is induced by the apotosis through special mechanisms including the activation of caspase-3. On the basis of this background, this study was designed to examine the cartilage protective and anti-apototic effects of Aralia Cordata in in vtro and in collagenase-induced arthritis rabbit model. To conduct in vitro study, chondrocytes culturedfrom rabbit knee joint were treated by 5 ng/ml IL-1a.For in vivo experiment, collagenase-induced arthritis (CIA) rabbit model was made via intraarticular injection with 0.25 ml of collagenase solution. Aralia cordata pharmacopuncture (ACP) was administrated on bilateral Dokbi acupoint (ST35) of rabbits at a dosage of 150 ${\mu}g/kg$ once a day for 28 days after the initiation of the CIA induction. In the study by using CIA rabbit model in vivo, ACP showed the inhibition of cartilage degradation in histological analysis. Aralia cordata also showed anti-apoptotic effect both in vitro and in vivo study. In chondrocytes treated by IL-1a, Aralia cordata inhibited caspase-3 activity and enhanced the proliferation of IL-1a-induced dedifferentiated chondrocytes. ACP showed the inhibition effect on the caspase-3 expression and activity from CIA rabbit model. This study indicates that ACP inhibits the cartilage destruction and the chondrocyte apotosis through downregulation of caspase-3 activity. These data suggest that ACP has a beneficial effect on preventing articular cartilage destruction in osteoarthrtis.

• Nutrition Research and Practice
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• 제13권6호
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• pp.480-487
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• 2019

BACKGROUND/OBJECTIVES: Osteoarthritis (OA) is a major public health issue in Japan and other countries, and foods that prevent or treat OA are in strong demand. Proteins and peptides in chicken meat and bones are known for being rich in functional and nutritional ingredients for the improvement of osteoporosis. We speculated that chicken legs, a food consumed in many regions of the world, may also contain such ingredients. In this study, we aim to (i) evaluate the effect of chicken leg extract (CLE) on the promotion of cartilage matrix production and (ii) identify the active ingredient in CLE that contributes to this function. MATERIALS/METHODS: Artificial CLE digest was prepared, and the acid mucopolysaccharide production-promoting activity of the CLE digest was evaluated by alcian blue staining of ATDC5 cells. CLE was orally administered to rabbits with burr holes in the knee joint of the femur, and the degree of regeneration of cartilage matrix was evaluated. Furthermore, we investigated orally administered CLE-derived peptides in human plasma using LC-MS. From measuring the acid mucopolysaccharide production-promotion activity of these peptides, a molecule considered to be an active ingredient in the CLE digest was identified. RESULTS: CLE digest promoted acid mucopolysaccharide production and facilitated regeneration of cartilage matrix in in vitro and in vivo experiments. Four peptides including phenylalanyl-hydroxyproline (Phe-Hyp) were detected as CLE-derived peptides in human plasma. The effect of CLE was inferred to be due to Phe-Hyp, which was confirmed to be present in the CLE digest. CONCLUSIONS: It was shown that CLE stimulated the production of articular cartilage matrix both in vitro and in vivo, and that CLE could be an effective food for preventing or treating OA. Furthermore, only Phe-Hyp was confirmed as the active compound in the CLE digest, suggesting that the activity of CLE was due to Phe-Hyp.

• 생약학회지
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• 제39권1호
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• pp.1-6
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• 2008

AIF has been formulated using three herbs known to have anti-inflammatory and anti-osteolytic effects. In this study, the potential therapeutic effects of AIF for osteoarthritis were assessed in vitro and in vivo. The effects of AIF on the cartilage and bone protection (MMP-13 expression, GAG degradation, OPG release) were examined, in vitro. In addition, the therapeutic effect of AIF was evaluated using a chemical-induced osteoarthritis rat model. Rats were injected with iodoacetate intraarticularly in one knee joint and treated with the oral administration of 100 mg/kg AIF-glucosamine once a day for 3 weeks. And then, destruction of cartilage and bone was evaluated by histopathological assessment. AIF significantly inhibited the production of MMP13 and GAG in a dose dependent manner in vitro. Also, AIF increased the production of OPG. In OA rat model, the AIF-glucosamine treated group reduced cartilage destruction, compared to vehicle or glucosamine treated group. AIF showed potent protective effects for the destruction of cartilage and bone, in vitro and in vivo. These results suggest that AIF contains effective compound(s) which may modify the progression of arthritis.

• 한방재활의학과학회지
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• 제25권2호
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• pp.37-50
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• 2015

Objectives The purpose of this study was to know the effects of Danggwisayeok-tang (Dangguisinitang) extract (DGSYT) on monosodium iodoacetate (MIA)-induced rat osteoarthritis. Methods For this purpose, rats were divided into 5 groups. Normal group was not injected with MIA and orally administered any medication. Control group was injected with MIA and not orally administered any medication. DGSYT100 group was injected with MIA and orally administered 100 mg/kg of DGSYT. DGSYT300 group was injected with MIA and orally administered 300 mg/kg of DGSYT. JoinsT group was injected with MIA and orally administered 20 mg/kg of Joins tablet. DGSYT100 and DGSYT300 groups were orally administered DGSYT during a week before and 3 weeks after based on the day MIA injected. The changes of hepatotoxicity, nephrotoxicity, relative hind paw weight distribution, cytokine in serum, cytokine messenger ribonucleic acid (mRNA) in joint tissue and histopathological observation (Hematoxylin & Eosin and Safranin-O staining) were measured. Results Alanine aminotransferase (ALT) levels of DGSYT100, DGSYT300 and JoinsT groups were increased significantly, but these results were within normal range. Aspartate aminotransferase (AST) and creatinine levels of all groups were not changed significantly. In the change of relative hind paw weight distribution, DGSYT300 and JoinsT groups were decreased significantly 14 and 21 days after MIA injected. Interleukin-$1{\beta}$ (IL-$1{\beta}$) and Interleukin-6 (IL-6), Leukotriene $B_4$ and Osteocalcin levels of DGSYT300 and JoinsT groups were decreased significantly. In measurement of IL-$1{\beta}$ and nitric oxide synthase-II mRNA relative quantitative of control, DGSYT100, DGSYT300 and JoinsT groups were decreased significantly. In measurement of TNF-${\alpha}$, IL-6 and Cyclooxygenase-2 mRNA relative quantitative of control, DGSYT300 and JoinsT groups was decreased significantly. In histopathological observation of knee, synovial tissue, cartilage and proteoglycan of DGSYT100, DGSYT300 and JoinsT were well preserved compared with control group. Conclusions According to the results, DGSYT has anti-inflammation and pain relief effects. So it should be suppressed progression of arthritis in MIA-induced osteoarthritis rat.

• 대한인간공학회지
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• 제30권4호
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• pp.525-534
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• 2011

This paper describes the current status of musculoskeletal disorders(MSDs) and its ergonomic risk factors, including the preventive intervention in terms of the working conditions and health promotion among agricultural workers. Korean agricultural workers have been exposed to the poor working condition. They have suffered from a variety of work-related injury and diseases more than the general population or the other occupational group. MSDs are one of the most prevalent problems threatening the agricultural worker's health and quality of life. Low back pain, osteoarthritis of hip and knee joint and carpal tunnel syndrome are well known MSDs that was found easily and frequently among agricultural workers. MSDs are worsened by poor ergonomic working conditions such as heavy weight lifting, repetitive movement and awkward posture which involves squatting with long sitting work on the ground, long working hours and heavy workload which are inherent in the greater part of agricultural works. Even though Korean agricultural workers are very weak population in terms of occupational safety and health, no many studies were conducted in the past and no many social and institutional supports were provided sufficiently. We need more interests and efforts in agricultural fields to improve its working condition and to promote health status of agricultural workers.

• 대한통합의학회지
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• 제7권2호
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• pp.59-67
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• 2019

Purpose : The purpose of this study was to analyze the dynamic range of motion (ROM) of pelvic and translation of center of mass (COM) when wearing different shoe insole lifts according to leg length discrepancy (LLD) during free speed gait. Methods : Thirty-five healthy adults were participated in this study. Kinematic data were collected using a Vicon motion capture system. Reflective and cluster 40 markers attached to participants lower extremities and were asked to walk in a 6 m gait way under three different shoe lift conditions (without any insole, 1 cm insole, and 2 cm insole). The pelvic ROM and COM translation in three planes were sorted using a Nexus software, and a Visual3D motion analysis software was used to coordinate all kinematic data. Results : There were significantly increased maximal pelvic elevation and total pelvic range in coronal plane when wearing a standard shoe with 2 cm insole lift during gait (p<.05). When wearing a standard shoe with 2 cm insole lift, the total range of the pelvic segment were significantly different in all three motion planes (p<.05). Conclusion : Although LLD of less than 2 cm develops abnormal movement pattern of the pelvis and may cause of musculoskeletal diseases such as low back pain, hip and knee joint osteoarthritis, therefore intensive various physical therapy interventions for LLD are needed.

• 대한관절경학회지
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• 제15권1호
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• pp.36-41
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• 2011

현재 반월상 연골 절제술 이후 슬관절 동통을 호소하는 젊은 환자에서 치료 방법은 제한적이다. 이런 환자들에서 연골판 결손은 관절염 발생의 중요한 요소로 작용하지만, 인공관절을 시행하기에는 적절한 연령이 아니다. 이러한 경우 최근 점차 증가하는 치료 방법은 동종 반월상 연골 이식술이다. 그러나 동종 반월상 연골 이식술에 관해서는 아직도 많은 의문점이 남아있다. 또한, 문헌상 보고되는 동종 반월상 연골 이식술의 결과는 대부분 작은 수의 환자만을 대상으로 하거나 간접적인 결과만을 보고하고 있다. 이러한 많은 요소들 때문에 각각의 결과를 분석하고 비교하는 것은 매우 어려운 일이다. 본 종설에서는 동종 반월상 연골 이식술의 결과에 관한 최근의 연구를 고찰하였다.

• 대한관절경학회지
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• 제16권2호
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• pp.167-174
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• 2012

목적: 관절경적 세척술 및 변연 절제술을 시행하고 배양 검사로 확진된 화농성 슬관절염을 가진 환자들에서, 단기적 임상 경과와 중장기적 기능적 결과를 확인하고, 이에 영향을 미치는 인자를 분석하였다. 대상 및 방법: 관절경적 변연 절제술을 시행하고, 배양검사상 양성으로 판명된 32예의 환자를 대상으로 하였으며, 평균 추시 기간은 41.6개월이었다. 감염에 대한 임상적 평가로 사망, 재발, 적혈구 침강 속도 및 C-반응성 단백 수치의 정상화 기간, 정주용 항생제 투여 기간, 재입원 여부를 조사하였다. 방사선학적 평가로 인공 슬관절 치환술, 관절염 진행 정도를, 기능적 평가로 modified Lysholm, Tegner activity 및 Korean version of the Western Ontario and McMaster Universities(K-WOMAC) score를 조사하였다. 결과: 황색 포도상 구균이 21예에서 동정되었고, 적혈구 침강 속도 및 C-반응성 단백 수치의 정상화 기간은 평균 78.0 및 67.6일이었다. 2예에서 사망하였고, 6예에서 재발하였다. 입원 중 재발은 만성 신부전이 동반된 환자에서 빈발하였다(P=0.034). 5례의 환자에서 재입원이 관찰되었고, 최초 내원시 방사선 검사상 관절염 정도가 심하고(P=0.032), 입원 중 수술 횟수가 많을 수록 재입원의 가능성이 높았다(P=0.006). 21례에서 관절염이 진행하였고, 최종 Kellgren-Lawrence 등급은 최초 내원시의 등급과 관련이 있었다(P=0.007). 5례에서 인공 슬관절 치환술을 시행받았으며, Lysholm score는 평균 53.5점, Tegner activity score는 평균 2.7점, K-WOMAC score는 평균 44.2점이었다. 결론: 관절경적 치료 후 최종 관절염 정도는 기왕의 관절염 정도에 의해 결정되었다. 기존 퇴행성 변화가 심한 환자에서 재입원이 많았고, 만성 신부전 환자에서는 재발이 많아 이들 환자들에서는 주의를 요한다.