• Journal of Plant Biology
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• v.14 no.2
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• pp.1-6
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• 1971

When germination beds of carrot seeds were treated with either 0.01M or 0.05M concentrations of Ca(NO3)2, CaSO4, MgSO4, K2SO4 and KH2PO4, an acceleration in the germination rate was observed in the groups treated with 0.01M KH2PO4 and 0.05M MgSO4 and 0.05M Ca(NO2)3. In earlier work by the author with acetone a similar result was observed and reported. The pH range in these experiments was maintained between 5.0 and 6.0. It was found that the groups treated with 0.05M K2SO4, 0.05M Ca(NO3)2, 0.05M Ca(NO3)2, 0.05M MgSO4, 0.01M KH2PO4, 0.01M Ca(NO3)2 germinated earlier than the control group. The acceleration of the germintion rate varied with the inorganic compounds used in the following descending order; 0.01M KH2PO4, 0.05M Ca(NO3)2, 0.05M K2SO4, 0.05M CaSO4 and 0.05M KH2PO4. As a result of these expriments, it occurs to the author that in the germination of carrot seeds some inorganic compounds appear to activate the osmotic function of carrot seeds causing acceleration in the germination rate.

• Journal of Environmental Science International
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• v.7 no.2
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• pp.209-216
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• 1998

Nanofiltration[NF45] and reverse osmosis membrane(HR98PP) separation treatment of dyestuff wastewater was carried out In order to separate relatively pure water from synthetic dyestuff wastewater, which consists of reactive dye, acid dye, basic dye, direct dye, and disperse dye. The experiments were performed by using the plate and frame membrane module. In the nanofiltration and reverse osmosis membrane separation, When the NaCl concentration was 0.1, 5.0, and 20.091, retention was 63.0, 46.0, 0.9%, respectively. When permeate flux was 125.0, 67.5, and 45.0 L/$m^2$ h, the osmotic pressure increased with Increasing the NaCl concentration. Permeate flux of two membranes Increased as temperature Increased due to segmental movement of polymer of the membrane and the rejection rate of dyestuff was decreased gradually. It was found that the rejection rate was about 95% in the nanofiltratlon, while the reverse osmosis membrane showed a high rejection rate of 99% under all temperature and pressures conditions.

• Journal of Environmental Science International
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• v.21 no.3
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• pp.267-276
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• 2012

Spaceflights results in the reduction of immune status of human beings and increase in the virulence of microorganisms, especially gram negative bacteria. The growth of Klebsiella pneumoniae is enhanced by catecholamines and during spaceflight, elevation in the levels of cortisols occurs. So it is necessary to know the changes in physiology, virulence, antibiotic resistance and gene expression of K. pneumoniae under microgravity conditions. The present study was undertaken to study effect of simulated microgravity on growth, morphology, antibiotic resistance and cross stress resistance of K. pneumoniae to various stresses. The susceptibility of simulated microgravity grown K. pneumoniae to ampicillin, penicillin, streptomycin, kanamycin, hygromycin and rifampicin were evaluated. The growth of bacteria was found to be fast compared with normal gravity grown bacteria and no significant changes in the antibiotic resistance were found. The bacteria cultured under microgravity conferred cross stress resistance to acid, temperature and osmotic stress higher than the normal gravity cultured bacteria but the vice versa was found in case of oxidative stress.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.451-462
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• 2022

Dioscorea bulbifera tubers contain several phytochemicals of pharmaceutical value. They have been traditionally used for treating various ailments, including postmenopausal symptoms. In the present study, we analyzed the direct effects of Dioscorea tuber extract on mouse spermatozoa. Its contraceptive effect was also evaluated by an intravaginal application before copulation. Mouse spermatozoa were cultured in vitro with various concentrations of the extract. After culturing, the spermatozoa were stained with fluorescein isothiocyanate peanut agglutinin or Coomassie blue to study the acrosome reaction, stained with trypan blue to study the viability, or treated with a hypo-osmotic medium to study the membrane damage. Estrous female mice were intravaginally injected with the extract and copulated with males. The extract induced acrosome exocytosis, viability loss, and membrane damage in a concentration-dependent manner. Female mice treated with the extract showed complete loss of fertility. These observations indicate that the Dioscorea bulbifera tuber extract could be used as a topical contraceptive. Infertility could be due to the precocious acrosome exocytosis of the spermatozoa or membrane damage.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.18-25
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• 2014

This study was conducted to investigated effects of water relation of mulching and trickle irrigation on the external and internal fruit quality in Satsuma mandarin grafted on trifoliate orange rootstock in a orchard assigned to randomly three groups; whole period of Tyvex mulching (TM), Tyvex mulching with trickle irrigation once a week from October 22 to harvesting season (WM) and non-mulching treatment (NM). The average soil moisture content in the TM was lower than the WM during the time of trickle irrigation from Oct. 21 to Nov. 28. The leaf water potential was at the level of ${\Psi}max$ of -1.5 to -2.5 MPa during whole period of Tyvex mulching treatment but gradually increased at the point of supplement of water. The water and osmotic potential in juice vesicle was decreased by drought but increased again in response to the supply of water in WM. The total soluble solids (TSS) in fruit juice was increased by drought stress, but diminished in response to supply of water after drought. The content of titratible acidity was increased by drought stress but gradually decreased due to supplement of water after drought, reached it at the level of 1%. It was suggested that the accumulation of the total soluble solids compensates the degree of active osmoregulation and the decrease in content of acidity accounts for the fast respiration and water uptake resulted of the water after drought.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.456-460
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• 2004

Effects of sigma factor (${\sigma}^{B}$) on biofilm formation in Listeria monocytogenes 10403S and ${\sigma}^{B}-deficient$ sigB null mutant were studied under high osmotic and low temperature conditions. In brain heart infusion (BHI) medium containing 6% NaCl, wild type 10403S and ${\sigma}^{B}-deficient$sigB null mutant formed biofilms of $6.83{\pm}0.38\;and\;5.33{\pm}0.45\;log\;cfu/cm^{2}$, respectively. L. monocytogenes 10403S in BHI medium containing 6% NaCl formed 4.7 times larger biofilm than without NaCl. Culture of L. monocytogenes 10403S and sigB null mutant at $4^{\circ}C$ did not show any significant differences in biofilm formation. The results suggest biofilm formation is activated by ${\sigma}^{B}$ and NaCl, whereas not affected by low temperature stress in L. monocytogenes 10403S. More studies are necessary to determine biofilm formation mechanism in osmotolerant L. monocytogenes.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.328-334
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• 2014

This study examined the effect of concentration polarization on permeate flux in forward osmosis (FO) membrane process for saline and sucrose solution. The reduction in permeate flux during the FO membrane process is largely due to the formation of concentration polarization on membrane surfaces. The flux reduction due to internal concentration polarization formed on the porous support layer was larger than that due to the external concentration polarization on the active membrane surface. Water permeate flux through the FO membrane increased nonlinearly with the increase in osmotic pressure. The water permeability coefficient was $1.8081{\times}10^{-7}m/s{\cdot}atm$ for draw solution on active layer (DS-AL) mode and $1.0957{\times}10^{-7}m/s{\cdot}atm$ for draw solution on support layer (DS-SL) mode in NaCl solution system. The corresponding membrane resistance was $5.5306{\times}10^6$ and $9.1266{\times}10^6s{\cdot}atm/m$, respectively. With respect to the sucrose solution, the permeate flux for DS-AL mode was 1.33~1.90 times higher than that for DS-SL mode. The corresponding variation in the permeation flux (J) due to osmotic pressure (${\pi}$) would be expressed as $J=-0.0177+0.4506{\pi}-0.0032{\pi}^2$ for the forward and $J=0.0948+0.3292{\pi}-0.0037{\pi}^2$ for the latter.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.12
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• pp.1760-1764
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• 2002

In order to elucidate the physiological function of circulating IGF-I on muscle protein synthesis in the chicken under malnutritional conditions, we administrated recombinant chicken IGF-I using a osmotic mini pump to fasted young chickens and measured the rate of muscle protein synthesis and plasma metabolite. The pumps delivered IGF-I at the rate of $22{\mu}g/d\{300{\mu}g{\cdot}(kg\;body\;weight{\cdot}d)^{-1}\}$. Fractional rate of protein synthesis in the muscle was measured using a large dose injection of L-[$2,6-^3H$]phenylalanine. Constant infusion of chicken IGF-I did not affect plasma glucose level. Significant interaction between dietary treatment and IGF-I infusion was observed in plasma NEFA and total cholesterol concentrations. When chicks were fasted, IGF-I infusion decreased plasma NEFA and total cholesterol concentrations. On the other hand, IGF-I administration did not affect plasma levels of both metabolites. Fasting reduced plasma triglyceride concentration significantly. IGF-I infusion also decreased the level of plasma triglyceride. Plasma IGF-I concentration of young chickens was halved by fasting for 1 d. IGF-I infusion using an osmotic minipump for 1 d increased plasma IGF-I concentration in fasted chicks to the level of fed chicks. Fasting decreased body weight and the loss of body weight was significantly ameliorated by IGF-I infusion. There was a significant interaction between dietary treatment and IGF-I infusion in the fractional rate of breast muscle protein synthesis. There was no effect of IGF-I infusion on muscle protein synthesis in fed chicks. Muscle protein synthesis reduced by fasting was ameliorated by IGF-I infusion, but did not reach to the level of fed control. Muscle weight of fasted chicks infused with IGF-I was similar to fasted birds without IGF-I infusion, which suggests that muscle protein degradation would be increased by IGF-I infusion as well as protein synthesis in fasted chicks.

• Journal of Nutrition and Health
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• v.46 no.4
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• pp.307-314
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• 2013

Resveratrol (RSV) exerts several beneficial effects on metabolism and metaflammation-related diseases, including diabetes and non-alcoholic fatty liver disease (NAFLD). The purpose of this study is to investigate whether RSV affects pathophysiology of diabetes and NAFLD as well as hepatic autophagy in a rodent model of diet induced obesity (DIO). DIO was induced in a subset of C57BL/6J mice fed a high fat (HF, 45% kcal fat) diet. After six weeks of HF diet treatment, RSV (8 mg/kg/day) was administered via an osmotic pump for a period of four weeks. Therefore, the experimental groups were as follows: 1) lean control (CON), 2) HF diet-induced obese control (HF), and 3) HF_RSV. Body weight and food intake were monitored daily. Fasting glucose, insulin, and adiponectin in serum and lipid profiles in serum and liver were analyzed. In addition, the autophagic process was investigated using transmission electron microscopy (TEM). Body weight and food intake were not affected by RSV treatment. Impaired glucose control accompanied by DIO was recovered with RSV as shown by lower levels of fasting serum glucose and insulin when compared with HF obese controls. In addition, RSV treatment resulted in increased levels of serum adiponectin, however, indices of lipid profile in serum and livers were reduced. Results of TEM analysis showed that a HF diet induced excessive autophagy with the presence of double-membrane autophagosomes, which was ameliorated by RSV. The regulatory effect of RSV on autophagy was confirmed by the altered LC3-II formation, which increased with a HF diet and was decreased by RSV treatment. These results suggest that RSV treatment improves glucose control and lipid profile and these beneficial effects may be mediated by an altered autophagic process.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.66-72
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• 2020

Alstroemeria plants were transformed by using an improved particle-gun-mediated transformation system. Friable embryogenic callus (FEC) induced from the leaves with axil tissues of Alstroemeria plant was used as the target tissue. Also, FEC was transformed with the bar gene was used as a selectable marker. In the case of plasmid pAHC25, 7.5% of the twice-bombarded FEC clumps showed blue foci, whereas the clumps with single bombardment showed only 2.3%. Additionally, a 90° rotation with double bombardment led to a higher frequency (6 times) of luciferase gene expression in PBL9780 than the control treatment. After 8 weeks of bombardment, more than 60 independent transgenic lines were obtained for pAHC25 and nearly 150 independent transgenic lines were obtained for PBL9780, all of which were resistant to PPT and demonstrated either GUS or luciferase activity. Regarding effect of osmotic treatment (0.2 M mannitol) with 7 different periods, the highest transient gene expression was obtained in 8 h before and 16 h after transformation in both pAHC25 and PBL9780. Compared with the control, at least three times more GUS foci and photons were observed in this treatment. With respect to different combinations of mannitol and sorbitol with 8 h before and 16 h after transformation, high numbers of transient and stable transgene expressions were observed in both 0.2 M mannitol and 0.2 M sorbitol used in the osmotic pre-culture. This combination showed the highest transformation efficiency in both pAHC25 (8.5%) and PBL9780 (14.5%). In the control treatment, only 10% of the FEC clumps produced somatic embryos. However, by using 0.2 M mannitol and 0.2 M sorbitol, the frequency of somatic embryos increased to 36.5% (pAHC25) and 22.9% (PBL9780). Of the somatic embryos produced, at least 60% germinated. Approximately 100 somatic embryos from these 210 independent transgenic lines from 2 plasmids developed into shoots, which were then transferred to the greenhouse. PCR analysis confirmed the presence of the bar gene. This is the report on the production of transgenic Alstroemeria plants by using particle bombardment with a high efficiency, thereby providing a new alternative for the transferring of gene of interests in Alstroemeria in the breeding program in the future.