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http://dx.doi.org/10.7744/kjoas.20220040

Dioscorea bulbifera tuber extract causes sterility in mice  

Jayaswori, Sharma (Central Department of Biotechnology, Tribhuvan University)
Sabina, Bhandari (Central Department of Biotechnology, Tribhuvan University)
Sarbesh, Rijal (Central Department of Biotechnology, Tribhuvan University)
Ramanuj, Rauniyar (Central Department of Biotechnology, Tribhuvan University)
Young-Joo, Yi (Department of Agricultural Education, College of Education, Sunchon National University)
Gaurishankar, Manandhar (Central Department of Biotechnology, Tribhuvan University)
Publication Information
Korean Journal of Agricultural Science / v.49, no.3, 2022 , pp. 451-462 More about this Journal
Abstract
Dioscorea bulbifera tubers contain several phytochemicals of pharmaceutical value. They have been traditionally used for treating various ailments, including postmenopausal symptoms. In the present study, we analyzed the direct effects of Dioscorea tuber extract on mouse spermatozoa. Its contraceptive effect was also evaluated by an intravaginal application before copulation. Mouse spermatozoa were cultured in vitro with various concentrations of the extract. After culturing, the spermatozoa were stained with fluorescein isothiocyanate peanut agglutinin or Coomassie blue to study the acrosome reaction, stained with trypan blue to study the viability, or treated with a hypo-osmotic medium to study the membrane damage. Estrous female mice were intravaginally injected with the extract and copulated with males. The extract induced acrosome exocytosis, viability loss, and membrane damage in a concentration-dependent manner. Female mice treated with the extract showed complete loss of fertility. These observations indicate that the Dioscorea bulbifera tuber extract could be used as a topical contraceptive. Infertility could be due to the precocious acrosome exocytosis of the spermatozoa or membrane damage.
Keywords
Discorea bulbifera; fertility; sperm acrosome; sperm membrane; sperm viability;
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Times Cited By KSCI : 3  (Citation Analysis)
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