• Title/Summary/Keyword: Osmotic Pressure

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Responses of Transgenic Tobacco Plants Overexpressing Superoxide Dismutase and Ascorbate Peroxidase in Chloroplasts to Water Stress (Superoxide Dismutase와 Ascorbate Peroxidase를 엽록체에 과발현하는 형질전환 담배의 수분스트레스에 대한 반응)

  • 최선미;권석윤;곽상수;박용목
    • Journal of Environmental Science International
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    • v.10 no.1
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    • pp.79-84
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    • 2001
  • To assess resistance of transgenic tobacco plants which overexpress superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts to water stress, changes in leaf water potential, turgor potential, stomatal conductance and transpiration rate were measured. Leaf water potential in all plants remained high up to day 4 after withholding water but thereafter decreased markedly. In spite of a remarkable decrease in leaf water potential, some of transgenic plants maintained higher turgor potential compared with control plant on day 12. In particular, the transgenic plant expressing MnSOD showed an outstanding maintenance in turgor pressure by osmotic adjustment throughout the experiment, resulting in high stomatal conductance and transpiration rate. However, among transgenic plants, osmotic potential was reduced more effectively in multiple transformants such as the double transformant expressing both MnSOD and APX, and the triple transformant expressing CuznSOD, MnSOD and APX than single transformants. Consequently, further research is needed to get general agreement on the tolerance of transgenic plants to water stress at different growth stages for each transgenic plant.

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The Effects of Osmogant and Binder in Membrane on Nifedipine Release from Osmotic Granule (니페디핀의 삼투성 과립에서 삼투염과 반투막내의 결합제 종류가 약물방출에 미치는 영향)

  • Jeong Sung-Chan;Cho Young-Ho;Kim Moon-Suk;Lee Bong;Khang Gil-Son;Rhee John-M.;Lee Hai-Bang
    • Polymer(Korea)
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    • v.30 no.2
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    • pp.112-117
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    • 2006
  • To improve the type error of osmotic tablet which is one of the drug delivery system, osmotic granule could be manufactured by fluidized bed coating. It has drug layer containing different amount of osmogant and is coated with membrane including different types of binder. We confirmed that the morphology of osmotic granule was different at each coating step. The more mont of osmotic agent, the faster drug release was observed due to increasing the driving force for drug release from osmotic granule. And drug release from osmotic granule coated with membrane using different types of binder was differed by solubility of binders to water. The formation of pore in membrane was confirmed by SEM and DSC Membrane using water soluble binder released more amount of drug. From these results, we assured that difference of osmotic pressure between the inside and the outside of granule and porosity of membrane have an effect on drug release from osmotic granule.

Water Relations Parameters of Heracleum moellendorffii Hance Obtained from Pressure-Volume Curves (P-V 곡선법을 활용한 어수리의 수분특성 분석)

  • Lee, K.C.;Kwon, Y.H.;Lee, K.M.;Han, S.K.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.19 no.1
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    • pp.91-97
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    • 2017
  • This study was carried out to establish a proper cultivation site and to diagnose the drought tolerance of Heracleum moellendorffii leaves by using pressure-volume curves. As a result of analysing data measured, the leaf of H. moellendorffii showed the osmotic pressure at full turgor (Ψosat) was -1.0MPa, and that at incipient plasmolysis (Ψotlp) -1.2MPa. Then, the value of maximum bulk modulus of elasticity Emax was 28MPa, showing the sightly strong drought tolerance of H. moellendorffii. Furthermore, the values of relative water contents RWCtlp and RWC* were above 88%, showing that the function of osmoregulation is somewhat better. Thus, responses to water relations such as Ψosat, Ψotlp, Emax, RWCtlp and RWC* of H. moellendorffii showed it's slightly high drought tolerance property.

Effects of Ginseng Saponins on Cholesterol Solubility (인삼(人蔘) Saponin류(類)가 Cholesterol 용해도(溶解度)에 미치는 영향(影響))

  • Ha, Choon-Ja;Kim, Sung-Ho
    • Korean Journal of Pharmacognosy
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    • v.15 no.3
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    • pp.134-138
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    • 1984
  • In this study the solubility of cholesterol was examined with ginseng saponin and chemical saponin used in the preparation of reaction mixture of 0.15M NaCl solution. It was shown that the solubility of cholesterol varied in the concentration of ginseng saponins and in the temperature and that the osmotic pressure and the surface tension was amenable to changes in the concentration of ginseng saponins and the incubation period of the reaction mixture. From the results, it was revealed that the solubility of cholesterol was dependent upon the incubated times and the concentration of ginseng saponins in 0.15mM NaCl solution and that ginseng saponins have the cholesterol solubilizing effect as evidenced in the physicochemical properties of mixed micelle formation and surface-activity.

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Effects of Light, Temperature, Water Changes on Physiological Responses of Kalopanax pictus Leaves(IV) - Characteristics of Leaf Water Relations Obtained from P-V Curve - (광, 온도, 수분 변화에 따른 음나무 엽의 생리반응(IV) - P-V 곡선에 의한 잎의 수분특성 -)

  • Han, Sang-Sup;Jeon, Doo-Sik;Sim, Joo-Suk;Jeon, Seong-Ryeol
    • Journal of Forest and Environmental Science
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    • v.22 no.1
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    • pp.71-75
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    • 2006
  • Water relations of Kalopanax pictus leaves obtained from the P-V curve. In the upper leaves of Kalopanax pictus seedlings, the original osmotic pressure at maximum turgor was -1.44 MPa, and the osmotic pressure at incipient plasmolysis point was -1.84 MPa, and the relative water content at incipient plasmolysis point was 78.2%.

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Comparison of Adjustments to Drought Stress Among Seedlings of Several Oak Species

  • Kim, Joon-Ho
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.343-347
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    • 1994
  • In order to compare the adjustment of 6 oak species to water stress, the components of water status, tissue elastic modulus, free proline content of leaves and morphological characteristics were determined in pot culture. uercus dentata and . mongolica responded effectively to drought with high root : shoot (R/S) ratio or maintenance of high turgor pressure by large and fast osmotic adjustment and . variabilis with maintenance of high turgor pressure by low elastic modulus under drought. Meanwhile, . aliena and . serrata responded effectively with low omotic potential (Ψo) at full saturation and . acutissima with long root in spite of rigid cell wall and high osmotic potential (Ψo) at full saturation. Proline content in leaves of . dentata, . mongolica and . aliena increased early and rapidly at high leaf water potential (Ψleaf). The results indicate that 6 oak species have adjustment different from each other to water stress.

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Studies on Photosensitive Resins.-Molecular Weight Determination of Isophthalic Acid-Cinnamic Acid-Glycerine Polycondensates (感光性樹脂에 關한 硏究-Isophthalic acid-Cinnamic acid-Glycerine 系 縮重合物의 分子量에 關하여)

  • Shim, Jyong-Sup;Lee, Boo-Sop
    • Journal of the Korean Chemical Society
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    • v.6 no.1
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    • pp.94-98
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    • 1962
  • The polycondensates of isophthalic acid-cinnamic acid-glycerine system were synthesized. The molecular weights of these products were determined by two methods, viscosity method and osmotic pressure methods. In the synthesis of resin, the reaction temperature was kept at 232$^{\circ}C$ and the mole ratio of the reactants was chosen as follows: Glycerine ;1.0 Isophthalic acid ;1.0 Cinnamic acid ;2.8 Excessive cinnamic acid prevented gelation of reaction mixtures. As the solution became very dilute, reduced osmotic pressure P/C (at the concentration of solution below 0.24g/100 ml of acetone) and reduced viscosity ${\eta}_{sp}/C$ (at the concentration of solution below 0.32 g/100 ml of acetone) increased rapidly. The correspondence between molecular weights determined by the two methods made Huggin's equation applicable to the prepared polymer. The values of K and a are $2.77{\times}10^{-5}$ and 1.063, respectively.

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A Study on Osmotic Fragility of the Red Blood Cell in Histamine-treated Rabbit (Histamine에 의한 적혈구 취약성의 변화에 관한 연구)

  • Ahn, Seung-Woon;Kim, Joong-Soo;Kim, Ki-Kon;Lee, Soon-Jai
    • The Korean Journal of Physiology
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    • v.9 no.1
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    • pp.33-37
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    • 1975
  • Adult rabbits were anesthetized with nembutal, 30 mg/kg. Carotid artery and jugular vein were exposed surgically and cannulated with polyethylene tubing. Arterial blood pressure was recorded via pressure transducer on the physiograph and $100{\mu}g/ml$ of histamine solution was infused through the jugular vein by using the constant infusion pump with a rate of 0.92 ml/min or 1.40 ml/min. Mean arterial blood pressure was maintained at $40{\sim}70 mmHg$ and hypotension was kept for 2 hours. After the termination of this period, blood was taken and osmotic fragility was mea sured immediately. Also, every sample of normal blood and shocked blood was incubated for 1 hour or 2 hours at $37^{\circ}C$ in order to see whether or not there was some influence of incubation. Furthermore to clarify which component was responsible for the change on the fragility, the mixtures of normal blood cells with shocked plasma and shocked blood cells with normal plasma were also incubated at $37^{\circ}C$ for one or two hours and fragility in such cases was measured. The data obtained were analysed by probit-plot method and the concentration of saline solution at which the hemolysis started to occur, 50% of blood cells were hemolysed and that at which the red blood cells hemolysed completely were determined. The values for the blood of hypotension stage were compared with those of the control blood. The results obtained were as fellows: 1. Osmotic fragility of red blood cell was increased in hypotensive state induced by histamine. 2. The differences of osmotic fragility after two hours of incubation were negligible both in normal blood and in that of hypotensive state. 3. Osmotic resistance of normal red blood cell incubated in shock plasma was less than that of shock red blood cell incubated in normal plasma. It was suggested that plasma in hypotensive state caused by histamine might be primarily responsible for the alteration of red blood cell fragility.

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Stabilization and Drug Release of Water/Oil/Water Multiple Emulsions : Effect of Glucose in the Outer Aqueous Phase on Osmotic Pressure Reduction (Water/Oil/Water 다중유화의 안정성과 약물 방출: 외부 수상에 포함된 글루코즈에 의한 삼투압 조절 효과)

  • Yoo, Youngtai;Lim, Eun-Jung;Kim, Tae-Yoon;Kim, Dong-Chul
    • Applied Chemistry for Engineering
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    • v.8 no.5
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    • pp.715-721
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    • 1997
  • W/O/W multiple emulsions were prepared comprising $MgSO_4$ in the inner aqueous phase as a model drug. The stability and drug release behavior of the multiple emulsions were studied using optical microscopy, viscometry and conductometry. Glucose was introduced in the outer aqueous phase to reduce the osmotic pressure gradient across the oil layer arising from the localization of drug molecules in the inner water phase. It was found that the presence of glucose was effective in stabilization of the multiple emulsions and in control of the release rate of drug more evidently when oil phase was partially hydrophilized with cetostearyl alcohol. This may be attributed to the fact that the migration of water accompanying the hydrophilic surfactant to the inner water phase was limited under a reduced osmotic pressure gradient and thereby slow down the destabilization of the oil/inner water interface.

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Effect of Microbially Induced Calcite Precipitation on Plant Growth (미생물에 의해 생성된 탄산 칼슘 침전이 식물 생장에 미치는 영향)

  • Kim, Tae-Young ;Nawaz, Muhammad Naqeeb;Do, Jinung ;Chong, Song-Hun
    • Journal of the Korean Geotechnical Society
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    • v.39 no.8
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    • pp.41-48
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    • 2023
  • Microbially induced calcite precipitation(MICP) is a novel cementation method meant to enhance soil engineering properties through the use of microorganisms. This study investigates the effect of different MICP concentrations on plant growth. Tall fescue seeds are grown in plant columns filled with Jumunjin sand. Following plant growth, the soil samples are treated with MICP via spraying method. The results indicate that the MICP-treated plants exhibit hampered growth compared with the untreated plants. pH and electrical conductivity(EC) tests are performed to analyze the changes in soil properties by MICP. The MICP-treated soils exhibit a pH = 7, similar to the untreated soil. However, the EC dramatically increases with the increase in the MICP concentration, which leads to an increase in the osmotic pressure of the soil surrounding the plant roots. Eventually, the higher osmotic pressure in MICP-treated soil hinders the absorption of water and nutrients in plant roots, thus inhibiting plant growth.