• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.102-102
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• 2002

• Proceedings of the Zoological Society Korea Conference
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• 2003.08a
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• pp.179.3-179
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• 2003

No Abstract, See Full Text

• Preventive Nutrition and Food Science
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• v.3 no.2
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• pp.198-202
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• 1998

The arbitrary primed polymerase chain reaction (AP-PCR) has been used to detect the genetic alternations in the related species. Simple and reproducible fingerprints of complex genomes can be generated using single arbitrary chosen primers and the PCR. The technique was applied to the Oryza species and characterized the relationship among three cultivars of rice species based on theresult of genomic DNA fingerprints. The results indicated that the polymorphism revealed in rice strains and the differences in the PCR product pattern could be represented for each strainis. There was many variationsin the PCR product pattern between cv. Dongin(japonica type)and cv.Hyangdo (indica type), and our chosen AP-primers can ge as markers for strain identification and verfication.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.4
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• pp.203-214
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• 2017

Environmental toxicity due to cadmium (Cd) pollution in croplands causes critical problems worldwide. Rice (Oryza sativa L.) is an important crop in Asia, including South Korea, and numerous studies have evaluated the relationship between Cd and antioxidants to alleviate Cd uptake from the soil into plants. However, information about the relationship between phosphorus (P) and antioxidants in rice seedlings is still limited with regard to Cd phytotoxicity. We therefore investigated the physiological responses of rice (Oryza sativa L. cv 'Dongjin') seedlings to Cd toxicity and the effect of P application on reactive oxygen species (ROS) and antioxidant changes. The exposure of rice seedlings to $30{\mu}M$ Cd inhibited plant growth; increased the contents of superoxide, hydrogen peroxide, and malondialdehyde; and induced Cd uptake by the roots and leaves. Application of P to Cd-exposed seedlings decreased Cd-induced oxidative stress by enhancing the capacity of ascorbate (AsA) production and ROS-scavenging, and decreased Cd transfer from the roots to the leaves. These results suggest that P application alleviated Cd-induced growth inhibition and oxidative damage by restricting Cd translocation from the roots to the leaves and maintaining sufficient levels of AsA.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.83.2-83
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• 2003

A full length cDNA, OsLEUZIP, encoding leucine zipper containing protein from rice EST of rice (0ryza sativa L. cv. Dongjin) treated Xanthomonas oryzae pv. oryzae 10331. OsLEUZIP contains 1,227 bp nucleotides and encodes a protein of 408 amino acid residues with predicted molecular weight of 47,229 Da. The deduced amino acid sequence of OsLEUZIP has consensus sequence of leucine zipper from PROSITE (PDOC00029), L-X(6)-L-X(6)-L-X(6) -L. OsLEUZIP gene were preferentially induced in rice during incompatible interaction with Xanthomonas oryzae pv. oryzae 10331 and Pyracuraria grisea KJ-301. Expression of OsLEUZIP gene was also induced by treatment of abiotics such as ethephon and ABA. Our data represented in this study suggesting that OsLEUZIP gene may play an important role in the rice defense-related. Further studies of this gene, overexpression in rice, yeast-two hybrid assay, electrophoretic mobility shift assay and northern blot analyses of transgenic plant, would be useful to elucidate the role of the OsLEUZIP gene in defense responses of rice.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.387-387
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• 2005

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.142-151
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• 2012

This study was carried out to evaluate genetic variation of early-heading rice (Oryza sativa L. cv. Dongjin 1) lines derived from gamma-ray ($^{60}Co$, 300 Gy) irradiation. The average heading date of the 5 early-heading lines in $M_7$ and $M_8$ generation was faster than that of untreated control as 11 (line ${\gamma}$-2), 10 (line ${\gamma}$-5), 6 (${\gamma}$-1 line), 5 (${\gamma}$-3) and 4 days (line ${\gamma}$-4), respectively. According to ISSR analysis, polymorphic rate of the early-heading lines (from 5.9% to 23.4%) was higher than that of control (4.3%). The result indicates that the gamma-ray promote variation at DNA level. When genetic variations of rps16-trnK region were evaluated by nucleotide analysis, nucleotide length of the rps16-trnK region was 664 bp in all the early-heading lines and control. Out of 5 sites of nucleotide transposition detected in the region, however, 2 sites were appeared only in the early-heading lines.

• Korean Journal of Weed Science
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• v.18 no.1
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• pp.20-27
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• 1998

This experiments were conducted to investigate the effects of fertilizer for herbicides phytotoxicity of rice(Oryza sativa L. japonica cv Dongjin). The shoot and root growth of rice were inhibited more by pyrazosulfuron-ethyl or pyrazosulfuronethyl+molinate than bensulfuron methyl or bensulfuron methyl+dimepiperate application in nitrogen or nitrogen mixed solution. In phosphate or phosphate mixed solution, rice growth were inhibited more by bensulfuron methyl or bensulfuron methyl+dimepiperate than pyrazosulfuron-ethyl or pyrazosulfuron-ethyl+molinate application. In solution mixed with nitrogen and phosphate or fertilizer three elements, rice shoots were more. inhibited by pyrazosulfuron-ethyl or pyrazosulfuron-ethyl+molinate treatments, roots were inhibited more relatively by bensulfuron methyl or bensulfuron methyl+dimepiperate treatments. In all fertilizer solutions, rice plant heights were reduced by dimepiperate and molinate applications, but root growth was reduced only by nitrogen and phosphate mixed solution. Rice growth in sulfonylurea or their mixed herbicide application were more inhibited in high :nitrogen concentration arid by phosphate exclusion than by nitrogen exclusion culture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.12-18
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• 2004

Activity of senescence-induced antioxidant enzymes in the detached rice seedlings (Oryza sativa L. cv. Dongjin) was examined. The levels of $\textrm{H}_2\textrm{O}_2$ content and peroxidase (POD) activity were gradually increased during leaf senescence, whereas catalase activity was decreased. The activity of superoxide dismutase (SOD) was increased, and ascorbate peroxidase (APX) and glutathione reductase (GR) were slightly increased until 3d and 4d of dark induced-senescence, and thereafter were decreased. The activation of all SOD isoforms showed a significant decrease after 6d and 7d. After 4d to 7d of dark senescence, there was a significant effect in enhancing the activity of APX-12 and -13 isoforms as compared with light, despite similar levels in total APX activity. GR-8 and -10 isoforms were more effective in leaf senescence at 4d to 7d, particularly with respect to dark-induced senescence. These results suggest that the metabolism of active oxygen species such as $\textrm{H}_2\textrm{O}_2$ is dependent on various functionally interrelated antioxidant enzymes such as catalase, peroxidase, SOD, APX and GR.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.97-101
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• 2001

The transport and allocation of photosynthetic assimilate is an important regulatory factor in plant productivity, In order to modify assimilate partitioning in rice, transgenic plants containing a potato sucrose transporter (SuT) gene were developed. Calli derived from rice seeds (Oryza sativa L. cv Dongjin) were cocultured with A. tumefaciens LBA 4404 harboring the SuT gene. Calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. Roots were induced from the putative transgenic shoots on rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 150%. Stable incorporation of the potato SuT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis.