• Title/Summary/Keyword: Oryza sativa

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Phytolith analysis and rice cultivation possibility from Myosa-ri Archeological Site, Haman-gun, Gyeongnam Province (경남 함안 묘사리 식물규소체 분석과 농경가능성)

  • Kim, Hyo-Seon;Yoon, Soon-Ock;Hwang, Sang-Ill
    • The Korean Journal of Quaternary Research
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    • v.25 no.2
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    • pp.39-48
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    • 2011
  • Phytolith analysis of sediments at the river mouth of valley bottom plains in Myosa-ri, Haman is performed. By the phytolith results, Phragmites and Oryza sativa are dominated in the lower horizon. An abundance of Paniceae and Andropogoneae indicates the active agricultural activities under wet conditions. Moreover, the results of archeological studies suggest the paddy fields in the Three States Age. The phytolith assemblages are dominated in the middle horizon and they gradually increase in the upper horizon. The rice cultivation layer is not proportional to total phytolith yield due to the dominance of Oryza sativa in the lower horizon. However, because the Oryza sativa are continuously observed over the entire sediments, it is suggested that there had been the agricultural activities for the entire periods.

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Biochemical Properties and Localization of the β-Expansin OsEXPB3 in Rice (Oryza sativa L.)

  • Lee, Yi;Choi, Dongsu
    • Molecules and Cells
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    • v.20 no.1
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    • pp.119-126
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    • 2005
  • ${\alpha}$-Expansins are bound to the cell wall of plants and can be solubilized with an extraction buffer containing 1 M NaCl. Localization of ${\alpha}$-expansins in the cell wall was confirmed by immunogold labeling and electron microscopy. The subcellular localization of vegetative ${\beta}$-expansins has not yet been studied. Using antibodies specific for OsEXPB3, a vegetative ${\beta}$-expansin of rice (Oryza sativa L.), we found that OsEXPB3 is tightly bound to the cell wall and, unlike ${\alpha}$-expansins, cannot be solubilized with extraction buffer containing 1 M NaCl. OsEXPB3 protein could only be extracted with buffer containing SDS. The subcellular localization of the OsEXPB3 protein was confirmed by immunogold labeling and electron microscopy. Gold particles were mainly distributed over the primary cell walls. Immunohistochemistry showed that OsEXPB3 is present in all regions of the coleoptile and root tissues tested.

Characterization of Uridine-Diphosphate Dependent Flavonoid Glucosyltransferase from Oryza sativa

  • Hong, Byoung-Seok;Kim, Jeong-Ho;Kim, Na-Yeon;Kim, Bong-Gyu;Chong, You-Hoon;Ahn, Joong-Hoon
    • BMB Reports
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    • v.40 no.6
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    • pp.870-874
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    • 2007
  • We cloned a uridine-diphosphate dependent glycosyl-transferase RUGT-10 from Oryza sativa. The recombinant enzyme was expressed by glutathione-S transferase gene fusion system in Escherichia coli. RUGT10 showed different regioselectivity depending on the structures of substrates (e.g. flavanone, flavonol, and flavone). Apparently, flavanone such as naringenin and eriodictyol gave one 7-O-glucoside while flavone and flavonol gave more than two products with preferential glucosylation position of hydroxyl group at C-3 position.

A Kinetic Study on the Phosphate Efflux in Rice Roots - Rhythmic Phenomena of Phosphate Flux - (벼의 인산유출기작에 대한 연구 -인산유출의 Rhythm현상)

  • 이길재;장남기
    • Asian Journal of Turfgrass Science
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    • v.11 no.1
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    • pp.9-22
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    • 1997
  • This study was performed to elucidate the mechanisms involved in efflux process of phosphate from intact and excised roots of Oryza sativa (Tongil). The rapid loss of phosphate during the first 40-minutes of treatment is mainly from the cytoplasmic fraction, and the gradual loss taking place afterwards is possibly reflecting the losses at the tonoplast. During the first 60 minutes, loss of phosphate from the excised roots treated with KCN was more rapid than the control but slower thereafter. The effect of $CaCl_2$ and KCl appeared to decrease the rate of phosphate efflux in excised roots of rice. The mechanism of phosphate efflux by rice roots seems to be passive and oscillatory in the short period. Key words: Oryza sativa(Tongil), Phosphate efflux.

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A Study on the Source of Reductants for Nitrate Reduction in Rice (Oryza sativa cv. Tongil) Roots (벼(Oryza sativa cv. Tongil) 뿌리에 있어서 Nitrate 환원에 필요한 환원력의 공급원에 관한 연구)

  • Chang, Nam-Kee;Choe, Hong-Gwan
    • The Korean Journal of Ecology
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    • v.7 no.2
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    • pp.85-90
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    • 1983
  • There was a decrease in nitrate reductase activity (NRA) measured in vivo in rice roots (Oryza sativa cv. Tongil) grown in anaerobic culture solution. But it was reversed by addition of malonate to the in vivo nitrate reduction assay medium. Malonate increased the in vivo NRA during 2-5 hours incubation and decreased it in longer incubation hours. In vivo NRA was stimulated by addition of NaHCO3 to the assay medium, but not by Na2CO3. The stimulation of NRA by NaHCO3 was not observed in shoot removed rice roots. It is suggested that CO2 from NaHCO3 is carboxylated by phosphoenol pyruvate carboxylase, results in increasing the malate contents in the roots, and stimulates the in vivo NRA. NADH needed in nitrate reduction is supported by malate oxidation. In rice roots, it seems probable that malate oxidation in the mitochondria is more important to nitrate reduction than malae oxidation in cytoplasm.

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Phytosterols from the Rice (Oryza sativa) Bran

  • Jung, Ye-Jin;Park, Ji-Hae;Shrestha, Sabina;Song, Myoung-Chong;Cho, Suengmok;Lee, Chang-Ho;Han, Daeseok;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.175-178
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    • 2014
  • Three phytosterols of rare occurrence, schleicheol 2 (1), $7{\beta}$-hydroxysitosterol (2), and $7{\alpha}$-hydroxysitosterol (3), were isolated from the n-hexane fraction of rice (Oryza sativa) bran, for the first time. Some nuclear magnetic resonance (NMR) assignments in the literatures are inaccurate. This study employed two-dimensional NMR experiments to identify exact peak assignments.

Antioxidant Compounds of Oryza sativa L (벼의 항산화성분)

  • Min, Byung-Sun;Lee, Hyeong-Kyu;Zee, Ok-Pyo;Moon, Hyung-In
    • Korean Journal of Pharmacognosy
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    • v.33 no.3 s.130
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    • pp.173-176
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    • 2002
  • In search for the plant-derived antioxidant compound, it was found that the EtOAc, BuOH extracts obtained from the leaves parts of Oryza sativa L. which exhibited a significant antioxidant activity from 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. Activity-guided fractionation on the basis of the inhibitory activity upon the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical cleavage. and repeated column chromatography afforded several antioxidant compounds from Oryza sativa L. The structures and stereochemistry of these compounds were established on the basis of analysis of spectra and some chemical transformations as follows: 5,7-dihydroxy- 8-methoxyflavone, $acacetin-7-O-{\beta}-rutinoside$, $pectolinarigenin-7-O-{\beta}-rutinoside$. At antioxidant activity test for isolated three compounds, antioxidant activity was showed too.