• Toxicological Research
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• v.35 no.3
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• pp.257-270
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• 2019

Silver nanoparticles (AgNPs) have been widely used in a variety of applications in innovative development; consequently, people are more exposed to this particle. Growing concern about toxicity from AgNP exposure has attracted greater attention, while questions about nanosilver-responsive genes and consequences for human health remain unanswered. By considering early detection and prevention of nanotoxicology at the genetic level, this study aimed to identify 1) changes in gene expression levels that could be potential indicators for AgNP toxicity and 2) morphological phenotypes correlating to toxicity of HepG2 cells. To detect possible nanosilver-responsive genes in xenogenic targeted organs, a comprehensive systematic literature review of changes in gene expression in HepG2 cells after AgNP exposure and in silico method, connection up- and down-regulation expression analysis of microarrays (CU-DREAM), were performed. In addition, cells were extracted and processed for transmission electron microscopy to examine ultrastructural alterations. From the Gene Expression Omnibus (GEO) Series database, we selected genes that were up- and down-regulated in AgNPs, but not up- and down-regulated in silver ion exposed cells, as nanosilver-responsive genes. HepG2 cells in the AgNP-treated group showed distinct ultrastructural alterations. Our results suggested potential representative gene data after AgNPs exposure provide insight into assessment and prediction of toxicity from nanosilver exposure.

• Journal of the Korean Society of Physical Medicine
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• v.14 no.3
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• pp.1-11
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• 2019

PURPOSE: This study compared the changes in the range of motion (ROM ) on the knee joint according to three different isometric contraction intensities when applying Evjenth - Hamberg stretching (EHS) to the hamstring muscles of healthy subjects. METHODS: Forty eight healthy subjects aged between 20 and 39 years were allocated randomly to four groups; performing EHS at 10% maximum voluntary isometric contraction (MVIC) group, EHS at 50% MVIC group, EHS at 100% MVIC group, and control group (CG) pre-, and post-intervention. The flexibility of the hamstring muscles was evaluated using a digital goniometer for measuring the knee joint ROM motion. A Chattanooga stabilizer was used to adjust the MVIC intensity by 10%, 50%, and 100%, respectively. RESULTS: These results show that the pre- and post-intervention knee joint ROM were significantly different in all four groups (p<.05). The post-intervention knee joint ROM showed a significant difference between the 100% MVIC group and non-MVIC group (p<.05). The knee joint ROM difference between the pre- and post-interventions was similar in the 10% MVIC and 50% MVIC groups but significant differences were observed among the other groups (p<.05). CONCLUSION: EHS of the hamstring muscles was effective in improving the knee joint ROM with an isometric contraction intensity of 10%, 50%, or 100% MVIC, and of these, 100% MVIC was most effective.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.25 no.1
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• pp.63-70
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• 2019

Background: The purpose of this study was to determine the effects of a pelvic compression belt on gait abilities and balance in subacute stroke patients. Methods: Twenty two patients with subacute were recruited and randomly assigned into two group: Two group offered conventional physical therapy and occupational therapy for five day. The group was composed of twelve patients. Participants in the experimental group were given the pelvic compression belt and conventional physical therapy, conventional occupational therapy, although conventional physical therapy and occupational therapy provided in the subjects in the control group. To assess the gait ability, the GAITRITE system was used and the Balance system SD was used to test balance. All measurements were performed before and after intervention. Results: The experimental group shows a significant improvement the cadence, velocity, step length and stride length in gait (p<.05) and show significant increase in the dynamic standing balance (p<.05). However, control group shows a significant improvement the cadence, velocity, step length and stride length in gait (p<.05), but shows no significant differences in dynamic standing balance. Furthermore, there were significant differences gait velocity, dynamic standing balance between two groups (p<.05). Conclusions: The results demonstrated that the elastic pelvic belt application is effective to improve gait velocity, dynamic balance in the subacute stroke patients. Thus, the elastic pelvic belt is seemed to be one of the potential methods to facilitate the active rehabilitation program for hemiplegia patients.

• Journal of IKEEE
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• v.23 no.3
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• pp.883-892
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• 2019

This study is the method which is adapted to control ankle joint movement for resolving the problem of gait imbalance in intervals where gait environments are changed and slope walking, as applying terrain-adaptive technique to intelligent above-knee prosthesis. In this development of above-knee prosthesis, to classify the gait modes is essential. For distinguishing the stance phases and the swing phase depending on roads, a machine learning which combines decision tree and random forest from knee angle data and inertial sensor data, is proposed and adapted. By using this method, the ankle movement state of the prosthesis is controlled. This study verifies whether the problem is resolved through butterfly diagram.

• Journal of The Korean Society of Integrative Medicine
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• v.7 no.3
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• pp.159-169
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• 2019

Purpose: The purpose of this study was to provide an effective method of exercise therapy for patients with cervicogenic headache. Methods: The subjects were divided into the following three groups according to the intervention received: cervix-stabilizing exercise (n=12, group 1), transcranial direct current stimulation (n=12, group 2), and cervix-stabilizing exercise combined with transcranial direct current stimulation (n=12, group 3). The intragroup and intergroup differences in muscle characteristics and neck disability index were compared and analyzed. Results: The comparison and analysis of the changes in muscle tone and post hoc analysis revealed statistically significant intragroup decreases in the upper trapezius and suboccipitals in groups I and III, and statistically significant intergroup differences in the upper trapezius, with greater changes in group III than in group II, and in the suboccipitals, with greater changes in groupIII than in groups Iand II. The comparison and analysis of the change in muscle stiffness and post hoc analysis revealed a statistically significanti ntra group decrease in the upper trapezius in group Iand suboccipitals in group III, and a statistically significant intergroup difference in both muscles, with greater change in group III than in group II. The comparison and analysis of change in neck disability index and post hoc analysis revealed a statistically significant intragroup decrease in all the three groups and a statistically significant intergroup difference, with greater change in group III than in groups I and II. Conclusion: The neck-stabilizing exercise and transcranial direct current stimulation were shown to be effective in decreasing the tone of the cervical muscles by stabilizing the cervical bone and improving muscle durability, and in improving the movement and limitation of joint range of motion by decreasing muscle tone and stiffness.

• BMB Reports
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• v.52 no.9
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• pp.572-576
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• 2019

Bisphosphonates are the mainstay of therapy worldwide for osteoporosis. However, bisphosphonates also have limitations. The objective of this study was to determine the role of miR-101-3p/Rap1b signal pathway in osteoclast differentiation after treatment with bisphosphonates. Our results revealed that miR-101-3p was an important regulator in bisphosphonates treated-osteoclasts. When miR-101-3p was down-regulated in bone marrow-derived macrophage-like cells (BMMs), the development of mature osteoclasts was promoted, and vice versa. However, alendronate decreased multinucleated cell number regardless of whether miR-101-3p was knocked down or over-expressed. TRAP activity assay confirmed the above results. Luciferase assay indicated that miR-101-3p was a negative regulator of Rap1b. Western blot analysis revealed that protein expression level of Rap1b in BMMs transfected with OV-miR-101-3p was lower than that in BMMs transfected with an empty vector. Rap1b overexpression increased TRAP-positive multinucleated cells, while Rap1b inhibition decreased the cell numbers. In vivo data showed that miR-101-3p inhibited osteoclast differentiation in ovariectomized mice while overexpressed of Rap1b blocked the differentiation. Taken together, our data demonstrate that miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates.

• Journal of The Korean Society of Integrative Medicine
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• v.9 no.4
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• pp.183-190
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• 2021

Purpose : The purpose of this study was to investigate the effect of side planks on the muscle thickness of the core muscles, external oblique, internal oblique, and transverse abdominis, and this study was conducted to compare whether side plank exercise according to the application of various unstable support surfaces increases the thickness by activating the action of the muscles. Methods : The subjects of this study were 30 healthy adults and were randomly and equally assigned to three groups by a random number table. All subjects were divided into three groups according to the application of an unstable support surface during the side plank(Group A = stable support, Group B = one unstable support, Group C = two unstable supports). The side plank exercise was performed 30 minutes a day, 3 times a week for a total of 4 weeks. The muscle thickness of the core muscle was measured before the intervention, 2 weeks, 4 weeks, and 3 times in total. All measured data were comparatively analyzed by repeated measures ANOVA and one-way ANOVA. The statistical significance level was set to .05. Results : The results of this study were as follows : 1. All muscles showed an interaction between training period and group. 2. There was a significant difference between the groups at the 2 weeks and 4 weeks of the internal oblique and transverse abdominis muscle measurements. Conclusion : The thickness of the abdominal muscle increased during the side plank exercise according to the support surface, and the thickness of the abdominal muscle increased the most during the side plank exercise on the unstable support surface. Therefore, it is thought that the addition of an unstable support surface will provide a more effective therapeutic effect on the thickness change of the abdominal muscle during side plank exercise.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.27 no.2
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• pp.45-54
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• 2021

Background: The purpose of this study was to investigate the effects of flexi-bar exercise on shoulder pain, dysfunction, quality of life, and range of motion of women with breast cancer surgery. Methods: A total of 42 female patients who had breast cancer were included in this study. By drawing lots, subjects were divided into a flexi-bar exercise group (FBG, n=21) and a general breast cancer group (GBG, n=18). The effects of flexi-bar exercise on patients were evaluated in terms of the visual analogue scale (VAS), the shoulder pain and disability index (SPADI), the European organization for the research and treatment of cancer quality of life questionnaire (EORTC QLQ-C30), the breast cancer specific EORTC quality of life questionnaire (EORTC QLQ-BR23), and range of motion (ROM), all of which were measured pre- and post- intervention followed by evaluation. Results: A significant increase was pain, functional disability level, quality of life, ROM within the two groups (p<.01). There were pain, functional disability level, quality of life, ROM post test then invention were significant between the two groups(p<.01). Conclusion: The flexi-bar exercise has a positive treatment effect on breast cancer patients.

• Physical Therapy Rehabilitation Science
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• v.10 no.2
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• pp.161-166
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• 2021

Objective: The purpose of this study was to quantitatively evaluate the Wii Balance Board (WBB)-based jump performance for the elderly and to confirm the difference in jump performance according to age. Design: Cross-sectional study. Methods: 40 young adults (aged 22.5±2.2 years) and 33 elderly (aged 75.1±5.2 years) without orthopedics disease participated in this study. Standing on the WBB then, with the signal "start," jump vertically to the maximum height at which you can jump, land on the force plate after jump and keep it standing on both feet. All subjects were required to practice the jump sufficiently before starting the measurement, each measuring three times, and the mean values were used. A one-minute break was provided between each trial. Evaluators waited within 1meter for every test to prepare for fall. Results: The vertical ground reaction force of elderly and young adults when jumping using WBB showed a significant difference (p<0.05) and demonstrated discriminant validity. Between two groups, there were significant differences in overall jump time (p<0.05), maximum value (p<0.05), minimum value (p<0.05), center of pressure (COP) pathlength (p<0.05), and flight time p<0.05). Conclusions: This study found that performing the vertical jump, the elderly showed longer jump time, lower vertical ground reaction force, COP pathlength and shorter flight phase than healthy young adults using WBB and demonstrated that as a measurement tool, WBB discriminated vertical jump performance between elderly and young adults.

• Korean Journal of Metals and Materials
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• v.50 no.4
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• pp.338-343
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• 2012

The early osseointegration of titanium (Ti) dental implants is related to the initial cell morphology. The morphology of the cells (mesenchymal stem cells, MSC) was observed on three different Ti disc surfaces, which were mechanically treated by polishing, blasting, and scratching. A non-directional surface (isotropic texture) was obtained by the blasting of HA grits on cp-Ti discs, and a unidirectional surface (anisotropic texture) was obtained by the scratching of SiC papers. The cell attachment and arrangement in the initial periods were quite similar, but those in the later periods were significantly affected by the texture of the cp-Ti discs. After 1 week, the blasted Ti discs showed non-directional arrangement or spreading of the cells, whereas the scratched cp-Ti discs showed unidirectional properties parallel to the direction of the scratched grooves on the surface. The surface roughness of the cp-Ti discs significantly affects cell proliferation. Cell proliferation on the blasted and scratched surfaces was about 60% and 40% higher compared to the control result (polishing group) after 1 week (P<0.05). Cell proliferation on the blasted and scratched surfaces after 1 week was slightly enhanced with increasing surface roughness. It is believed that the direction of cell attachment and arrangement is closely related to the surface texture of the substrate surfaces, but cell proliferation after a relatively long period of time is directly enhanced by the surface roughness, not by the surface texture.