• Korean Journal of Plant Taxonomy
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• v.35 no.4
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• pp.295-311
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• 2005

Starch gel electrophoretic studies using 24 populations of five Korean Orostachys species were conducted to investigate allozyme variation and to test hypotheses of systematic relationships among species. The resulting phenogram showed that the populations of five Korean Orostachys species were divided into two major groups. And they were concordant with molecular and morphological data in suggesting that Orostachys was divided into two groups corresponding to the subsect. Appendiculatae and subsect. Orostachys. The low genetic identities among Korean Orostachys species indicated that the species of Orostachys have diverged gradually through the model of geographical species. Comparing the previous genetic data from the species with similar life history and mode of reproduction, most of Korean Orostachys species revealed a significant low genetic variation, while the widespread O. japonicus showed a relatively high genetic variation among the Korean species. This kind of genetic variation pattern might be the results of the isolated habitats, limited numbers of individuals within the populations, destruction of habitats, inbreeding and asexual reproduction in Korean Orostachys populations. The Jungdongjin population (POP 21) of O. malocophyllus was genetically unrelated with remaining populations of the same species, and this interpretation was consistent to the results from the previous palynological and morphological studies. Our allozyme data supported the taxonomic treatment of recently proposed taxa, O. iwarenge (Makino) Hara for. magnus and O. margaritifolius.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.9-14
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• 2011

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves of nine commercial Orostachys plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Partial least square discriminant analysis (PLS-DA). The dendrogram based on hierarchical clustering analysis of these PLS-DA data separated the nine Orostachys species into five major groups. The first group consisted of O. iwarenge 'Yimge', 'Jeju', 'Jeongsun' and O. margaritifolius 'Jinju' whereas in the second group, 'Sacheon' was clustered with 'Busan,' both of which belong to O. malacophylla species. However, 'Samchuk', belong to O. malacophylla was not clustered with the other O. malacophylla species. In addition, O. minuta and O. japonica were separated to the other Orostachys plants. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from leaves represented the most probable chemotaxonomical relationship between commercial Orostachys plants. Furthermore these metabolic discrimination systems could be applied for reestablishment of precise taxonomic classification of commercial Orostachys plants.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.5-13
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• 2019

Orostachys species grow from anywhere in Korea. This variety, which lives in the harsh areas of the beach and mountains, is known to have as much change as it was isolated. It has been used as a medicine for a long time, and now the flower is beautiful and has been developed for horticulture. As a result of morphological characteristics and component analysis of three Orostachys species, the following results were obtained. 1. Orostachys species showed two types of leaves according to the growing environment. O. japonica (Maxim.) A. Berger and O. minuta (Kom.) A. Berger were lanceolate, and O. chongsunensis Y. N. Lee was obovate. 2. The leaves were all green. However, some of the O. japonica (Maxim.) A. Berger had white powder. Some of the O. minuta (Kom.) A. Berger was green with red spots on the back. O. chongsunensis Y. N. Lee showed strong gray before flowering but weak green when flowering. 3. The thorns of the leaves were O. japonica (Maxim.) A. Berger and O. minuta (Kom.), but O. chongsunensis Y. N. Lee was not. 4. Whether it was a leaf dot or not, there were dots in O. japonica (Maxim.) A. Berger and there were dots on the O. minuta (Kom.) and the O. chongsunensis Y. N. Lee. 5. The flowers were all white, and the stamens were red in O. japonica (Maxim.) A. Berger and O. minuta (Kom.) A. Berger and white in O. chongsunensis Y. N. Lee. 6. It was confirmed that the ingredients in Orostachys species contained kaempferol, quercetin, flavonoid etc., which have high antioxidant activity.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.157-162
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• 2001

Korean native Orostachys species collected from 22 regions investigated morphological characters.O. japonicus species(No.3∼No.9) were similar leaf morphology and characterized by lanceolate leaves, cuspidate leaf apex.O. malacophyllus species(No.12∼No. 17) were morphologically characterized by obovate leaves and acute leaf apex. This species could be differentiate from other species by no thorn and leaf outlines formed by densely populated red dots.O. iwarenge species(No.18∼No.22) were diversified, such as obovate and elliptical leaves with acute, obtuse and round leaf apices. However, this species could be differentiate from other species bl'no thorn and grey powdery green colored leaves. Species collected from Maemuldo(No. 10) and Pocheon(No. 11) was assumed that these 2 species could be the new species which were not named classified.

• Horticultural Science & Technology
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• v.19 no.2
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• pp.159-162
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• 2001

The genetic relationships of Korean native Orostachys species collected from various regions were analysed using random amplified polymorphic DNA (RAPD) method. Eighteen Orostachys species analysed with UPGMA were clustered into three groups A, B, and C. However, four species were not clustered into any group. O. iwarenge species in group A (No. 18-No. 22) showed low similarity with 66.3-73.9% according to the cluster analysis. O. malacophyllus species in group B (No. 12-No. 17) showed low similarity with 66.7-83.7% according to the cluster analysis. The similarity coefficient value of O. japonicus (No. 3-No. 8) except Anmyeondo collected variety (No. 9) showed higher level with 84.2-92.3% than O. iwarenge or O. malacophyllus. Therefore, O. japonicus is thought to be genetically stable, and have less regional variation.

• Natural Product Sciences
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• v.18 no.1
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• pp.32-38
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• 2012

The herbs of Orostachys japonicus (Crassulaceae) have been used to treat gastric cancer, gastric ulcer or hemorrhage. Flavonoid glycosides, mainly kaempferol (Kp)- and quercetin (Qc) glycosides, have been isolated from O. japonicus; however, no quantitative information on those flavonol glycosides and no peroxynitritescavenging activity of the Orostachys extracts have been reported. In this study, Kp- and Qc glycosides were qualitatively and quantitatively analyzed by high-performance liquid chromatography (HPLC) in eight Orostachys and a Meterostachys species including O. japonicas, O. margaritifolius, O. chongsunensis, O. minuta, O. ramosus, O. malacophylla, O. latiellipticus, O. iwarenge, O. iwarenge for. magnus, and Meterostachys sikokiana distributed or cultivated in Korea. Distinctively, O. margaritifolius contained two flavonol 3,7-di-O-glycosides of Kp 3,7-di-O-glucoside and Kp 3-rhamnosyl-7-glucoside, but O. japonicus had two flavonol 3-O-rutinosides, Kp 3-rutinoside and Qc 3-rutinoside. The three species of O. margaritifolius (24.36 mg/g MeOH extract), O. japonicus (21.28 mg/g), and O. minuta (19.50 mg/g) showed relatively higher flavonoid contents. The flavonol glycosides were analyzed using eight standard compounds (Kp, Qc, Qc 3-O-rhamnoside, Qc 3-O-glucoside, Kp 3- O-rutinoside, Qc 3-O-rutinoside, Kp 3-O-rhamnosyl-7-O-glucoside, Kp 3,7-di-O-glucoside). The present HPLC method was validated to verify the linearity, precision, and accuracy. In addition, the peroxynitrite-scavenging activity was also discussed.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.359-371
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• 2003

Numerical analyses using 28 morphological characters from 14 populations of four Korean Orostachys species were conducted to investigate infra-specific variation and to test species relationships. The resulting phenogram recognized three distinct clusters, and the populations of O. malacophyllus, O. iwarenge and O. minutus were closely related each other. The populations of O. japonicus from Gaeksanri (I) and Gadeuk-do were isolated from the remaining populations of the species, and it suggests that the ranges of morphological variation within the species are significant, and related to the previous cytological variation. The Jungdongjin population of O. malacophyllus was not grouped together with the same species, but was clustered with the populations of the O. japonicus, and suggested that it seems to be an unrelated population in O. malacophyllus. The results of the PCA analysis recognized two distinct groups: the populations of O. japonicus and remaining species populations. The populations of O. malacophyllus, O. minutus and O. iwarenge placed within the closely related species.

• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.118-125
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• 2021

In this study Orostachys japonica A. Berger used is a medicinal herb that has long been used as a folk remedy for cancer treatment. In this study, the antioxidant efficacy of the ethyl acetate fraction of Orostachys japonica A. Berger was confirmed. The results of the Orostachys japonica A. Berge ethyl acetate fraction of antioxidant activity assays showed Antioxidant effect of Orostachys japonica A. Berger EtOAc fraction extract at 0.10 mg/mL was showed a DPPH radical scavenging rate of 78.54% and ABTS+ radical scavenging rate of 73.48%. Also, the toxicity result of Orostachys japonica A. Berger EtOAc fraction extracts using alternative experimental animal model zebrafish, confirmed a 100% the survival of the zebrafish embryo was shown that there was no coagulation and no hatching delay at all concentrations. also ROS generation induced by UV-B irradiation was confirmed that the fluorescence intensity decreased as a whole in all larvae treated with Orostachys japonica A. Berger EtOAc fraction extracts. In particular, it was confirmed that ROS generation was effectively suppressed by showing a 35.7% reduction rate compared to the positive control at a concentration of 3 ㎍/mL. These results were confirmed that Orostachys japonica A. Berger EtOAc fraction extracts has the possibility of application in the cosmetics field as a natural antioxidant.

• 한국전자현미경학회:학술대회논문집
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• 1996.05a
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• pp.32-33
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• 1996

• 한국전자현미경학회:학술대회논문집
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• 2000.05a
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• pp.26-26
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• 2000