• Journal of Plant Biology
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• v.30 no.1
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• pp.21-30
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• 1987

The effects of abscisic acid(ABA) spraying for 12 weeks on the stomatal types and frequencies of O. malacophyllus leaves were summarized as follows. ABA inhibited the growth of O. malacophyllus. The prominent effect of ABA on the epidermal structure was the promotion of senescence such as thickness of cell walls, smooth sinuosity of cell walls, and large size of epidermal cells. The stomatal frequency was decreased to 23% by 10$\mu\textrm{g}$ ml-1 ABA and to 48% by 100$\mu\textrm{g}$ml-1, and also the stomatal size was more or less smaller than that of control. The developing secondary stomatal mother cell was not found in both 10 and 100$\mu\textrm{g}$ml-1ABA, but the arrested secondary stomatal mother cell was rarely found in 10$\mu\textrm{g}$ ml-1 ABA. The formation of normal stomatal types such as helico-eumesogenous and aniso-eumesogenous was found in both 10 and 100 $\mu\textrm{g}$ ml-1 ABA asin well as control. Also nine abnormal stomatal types were found, and the frequencies were promoted to 6% by 10 $\mu\textrm{g}$ ml-1 ABA and to 17% by 100 $\mu\textrm{g}$ ml-1 ABA. Among these abnomal stomata, four types such as aborted stomata, single-aborted guard cells, arrested stomata, and modified stomatal complexes were found in control as well as in 10 and 100 $\mu\textrm{g}$ ml-1 ABA, but five types such as wrenched stomata, unequal stomata, wavy guard cells, guard cells overlapped by guard cells, and dissolved cell wall stomata were found in both 10 and 100 $\mu\textrm{g}$ ml-1ABA. The modified stomata complexes were abnormal stomatal types which were newly found and also were varied in types.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.605-611
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• 2008

Antioxidant activity of wa-song (Orostachys japonicus A. Berger) was analyzed to clarify the influence of extractive solvent and drying method such as sun, hot-air and freeze drying. The contents of total phenols and flavonoids were significantly higher in 95% ethanol extracts than water extracts. Ability of reducing power and DPPH radical, hydroxyl radical and nitrite scavenging ability were higher in the order of hot-air dried sample> freeze dried sample> sun dried sample and these abilities were also higher in 95% ethanol extracts than water extracts. In conclusion, antioxidant activities of wa-song extracts were in proportion to the contents of total phenols and flavonoids. Also, hot-air drying is the superior method for the enhancement of antioxidant activity of wa-song.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.250-254
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• 2005

The contents of flavonol glycosides, $kaempferol-3-O-{\beta}-D-glucoside$(1), $kaempferol-3-O-{\beta}-D-glactoside$ (2), $kaempferol-3-O-{\beta}-D-rhamnoside$ (3), $quercetin-3-O-{\beta}-D-glucoside$ (4) and $quercetin-3-O-{\alpha}-D-rhamnoside$ (5) in the houseleeks controlled by night-break, day-length control, and temperature during overwintering were determined to be compared with those in wild one. The contents of the flavonol glycosides 1-5 in the houseleeks were decreased roughly with warming during overwintering, and increased with longer light duration under the day-length control experiments. While warming functioned negatively on the production of the flavonol glycosides in the houseleek, longer light irradiation did positively during overwintering.

• Journal of Life Science
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• v.28 no.7
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• pp.819-826
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• 2018

Orostachys japonicus (OJ) is a medicinal herb with immunoregulatory, anti-aging, anti-oxidative, and many other therapeutic properties. The purpose of this study was to elucidate the anti-cancer property of cultivated OJ. SW480 cell viability was significantly reduced by cumulative exposure to OJ extract. We also observed inhibitory effects of OJ after 72 hr through the growth and migration of SW480 cells using scratch assay. SW480 cells in OJ-free medium began to move into the scratch site at 24 hr; however, cells in medium containing OJ did not migrate into the scratch site until 48 hr. Male C57BL/6 mice (4 weeks old) were orally administered OJ extract for 31 days before injection of SW480 cells. At 7, 14, and 28 days after subcutaneous injection of SW480 cells, tumor weight and volume were analyzed. The body weight of the OJ-treated group was continuously increased during administration of the OJ extract relative to the control group. Injection of SW480 cells caused a reduction in body weight in all groups; however, the OJ-treated group exhibited a significant increase in body weight after 14 days. Tumor weight and volume were lower in the OJ-treated group than in the control group after 28 days. Although these results suggest that OJ suppresses the proliferation and migration of human colon cancer cells, additional studies are required to provide preclinical evidence before launching clinical trials evaluating OJ as an anti-cancer biohealth product.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.913-920
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• 2010

This study was conducted to improve the seed germination by low temperature and $GA_3$ in $Orostachys$ $japonicus$ A. Berger (Jirisan) and $O.$ $iwarenge$ (Mak.) Hara (Jejuyeonhwa), Korean native plants. Observation of the seeds using a stereo microscope showed that all seeds of the two species have wrinkled surface and oblong shape. Seed size ranged 0.77-1.00/0.25-0.37 mm (length/width), indicating that the seeds are minute seeds. When the seeds of two $Orostachsis$ species were sown into petri-dish and placed in a plant growth chamber of 10, 15, 20, or $25^{\circ}C$, 'Jirisan' showed seed germination below 20% at all temperatures and 'Jejuyeonhwa' 80% at only $10^{\circ}C$. Seed germination of 'Jirisan' increased up to 44% at $10^{\circ}C$ by low temperature ($4^{\circ}C$) storage for 10 days, but decreased again at storage for more than 20 days. The seeds of 'Jejuyeonhwa' showed a large increase in seed germination by low temperature for 20-30 days, which was 95% at $10^{\circ}C$, but low temperature for more than 40 days significantly decreased seed germination. Dipping treatment in GA3 solution of $50-400mg{\cdot}L^{-1}$ for different periods (3, 6, 12, and 24 hrs) remarkably improved germination rate and speed in both species, 80-100% in 'Jirisan' and 90-100% in 'Jejuyeonhwa' at all concentrations and dipping times used in this study.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.597-603
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• 1997

Orostachys japonicus, Wasong as herb medicine, has been artificially cultivated as an anti-tumor medicinal. The experiment was carried out to examine the effect of natural daylength as control or night-break treatment (NB) imposed at June 20, July 18 or Aug. 15 on its growth, dry weights of leaf and bract, stem, floret and root, and morphological characters including bolting and floret flowering. After a plant was grown in a 15cm plastic pot containing a 2 : 1 soil : peat moss mixture on May 23, three treatments with above differing night-break had been imposed around midnight up to Nov. 7. The plants were sampled 3 times at the same day forced to night-break and then done 6 times by 2-week interval after the final NB. Plant height and inflorescence length of all the NB increased with delayed NB but declined in comparison with the natural daylength. No. of leaves including bracts showed similar response to plant height although NB given before July 18 showed less leaves and bracts. Stem diameters of NB were continuously increased to middle Sept. to middle Oct. while that of natural daylength decreased after middle Oct. Natural daylength or NB given on Aug. 15 had greater fraction, shoot and total dry weights resulting from increment of leaf and bract up to Aug. or of floret, stem and root after Sept. The earlier NB, the later formation of florets and the less number of flowering florets whereas in natural daylength florets on inflorescence begun to be formed from middle Sept. were sharply increased up to middle Oct. when all the plants were flowered. Bolting was not formed in the plant of the earliest NB of June 20, and thereby no anthesis of florets up to early Nov. It was concluded that year-round cultivation of Orostachys japonicus plants was possible through controlling the NB timing because its bolting and flowering of florets separately occurred.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.584-585
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• 2006

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.582-583
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• 2006

• Korean Journal of Pharmacognosy
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• v.40 no.1
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• pp.51-58
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• 2009

The anti-hyperlipidemic effects of an ethyl acetate fraction from Orostachy japonicus in a high lipid diet-induced hyperlipidemic rat model were assessed. The fraction, which contained kaempferol, astragalin, and isoquercitrin, was associated with significant weight loss and reduction of lipid contents in serum and liver tissues. The fraction, which contains mainly flavonoids, diminished the levels of malondialdehyde and hydroxyl radical, and increased the anti-oxidative enzyme activities of superoxide dismutase, catalase, and glutathione peroxidase. Reduced bleeding and plasma clotting times resulting from the administration of the ethyl acetate fraction may reduce cardiovascular disease associated with hyperlipidemia.

• Journal of Wellbeing Management and Applied Psychology
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• v.4 no.3
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• pp.39-44
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• 2021

Purpose: In this study, skin and lotion containing 10% FRO were prepared to compare the skin improvement effects. Research design, data and methodology: After using cosmetics containing FRO ingredients, analysis was performed for 6 weeks. Results: As a result of measuring the moisture and elasticity of the pores, significant results were obtained only in the pores when used for 2 weeks between the experimental group and the control group (p<.001). Also after 4 weeks of use, moisture (p<.05) showed significant results, and after 6 weeks of use, moisture (p<.01) and elasticity (p<.001) showed significant.