• Molecules and Cells
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• v.19 no.3
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• pp.436-444
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• 2005

We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.

• Korean Security Journal
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• no.52
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• pp.43-70
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• 2017

Terrorist organizations that take advantage of the occasion of international events are changing from targeting specified people to attacking general public, raising the anxiety of the audience and participating countries. Therefore, the host country intends to improve its status as the host by ensuring safety and crisis management at any cost. As a country with a wealth of experience of hosting international events, Korea takes the burden of the security at Pyeongchang Winter Olympics. Consequently, to successfully host this winter Olympics, the organizing committee should bear in mind that Korea is no longer a safe country regarding terrorist attacks and take countermeasures against various crimes and terrorism that may arise during the Winter Olympic Games. This study overviews various measures as follows. First, the change of counter-terrorism strategy is necessary. Second, the safety entity and safety measures for Pyeongchang Olympic Games should be established. Third, private security companies should be actively utilized. Fourth, the safety management training for private security and safety personnel should be strengthened. Lastly, it is necessary to establish a rapid and organic preemptive safety management system to cope with safety and crisis effectively at Pyeongchang Winter Olympics and other international events that will be held in the future.

• The Journal of the Korea Contents Association
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• v.18 no.6
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• pp.63-72
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• 2018

This study was conducted to explore the impact of learning strategies on the level of participation in the online collaborative learning process. To do this, We studied 91 students who took professorship courses at A university in the Seoul metropolitan area. At the beginning of the semester, the learning strategies were measured through MSLQ, rehearsal, elaboration, organizing, critical thinking, metacognition, learning management, effort control, peer learning, and seeking help. Next, cooperative tasks were carried out to measure the interaction, and group composition consisted of 4-5 persons. The level of participation was measured by scores given to the messages created for interaction. The process of collaborative learning was divided into the steps of identifying learning goals, learning plans, performing individual learning, sharing learning results, and writing reports. The effects of learning strategies on participation level were analyzed through multiple regression analysis (stepwise selection method). As a result, the learning goal step influenced the highest level of metacognition, and the learning plan is the management of the learning time, the demonstration of the learning execution, the adjustment of the effort, the acquisition of help, the collegial learning, Writing was influenced by organization, elaboration, critical thinking, and critical thinking, metacognition, and elaboration.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.4
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• pp.76-91
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• 2017

The Area of a domestic railway station is designed based on the 4-step traffic demand forecasting model with the average daily passenger count as one of its parameter. However, nowadays, due to increasing rate of railway station's function, the non-passengers are increasing. In order to consider those non-passengers who aren't using trains, assumed volume are added to the average daily passenger count of station to estimate the area, but the criteria being applied has no concrete basis. Therefore, this study aimed to recalculate the increasing non-passenger rate based on actual survey data of station users in any type of railway station to obtain the optimum area. Subsequently, the the design area was performed through pedestrian simulation. According to the result of the simulation, it was found that the total space of the exciting railway stations can be reduced up to 45% and will still satisfy the level of service(LOS) requirement.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.1
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• pp.133-140
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• 2012

This paper suggests an algorithm that can shorten the complexity $O(n^2)$ of Dijkstra algorithm that is applied to the shortest path searching in real-time GPS Navigation System into an up-to-date O(n). Dijkstra algorithm manipulates the distance of the minimum length path by visiting all the nodes from the starting node. Hence, it has one disadvantage of not being able to provide the information on the shortest path every second, in a city that consists of sophisticated roads, since it has to execute number of node minus 1. The suggested algorithm, firstly, runs by means of organizing the set of out-neighbourhood nodes at each level of the tree, and root node for departure node. It also uses a method of manipulating the distance of the minimum path of all out-neighborhoods and interior of the out-neighborhoods. On applying the suggested algorithm to two sophisticated graphs consisted of bi-direction and uni-direction, we have succeeded to obtain the distance of the minimum length path, just as same as Dijkstra algorithm. In addition, it has an effect of shortening the time taken 4 times from number of node minus1 to number of level minus 1. The satisfaction of the drivers can be increased by providing the information on shortest path of detour, every second, when occurs any rush hour or any traffic congestion due to car accident, by applying this suggested algorithm to the real-time GPS system.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.4 s.310
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• pp.74-86
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• 2006

This paper proposes a robust and computationally efficient algorithm for automatic video object segmentation. For implementing the spatio-temporal segmentation, which aims for efficient combination of the motion segmentation and the color segmentation, an SOM-based hierarchical clustering method in which the segmentation process is regarded as clustering of feature vectors is employed. As results, problems of high computational complexity which required for obtaining exact segmentation results in conventional video object segmentation methods, and the performance degradation due to noise are significantly reduced. A measure of motion vector reliability which employs MRF-based MAP estimation scheme has been introduced to minimize the influence from the motion estimation error. In addition, a noise elimination scheme based on the motion reliability histogram and a clustering validity index for automatically identifying the number of objects in the scene have been applied. A cross projection method for effective object tracking and a dynamic memory to maintain temporal coherency have been introduced as well. A set of experiments has been conducted over several video sequences to evaluate the proposed algorithm, and the efficiency in terms of computational complexity, robustness from noise, and higher segmentation accuracy of the proposed algorithm have been proved.

• The Research Journal of the Costume Culture
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• v.14 no.1
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• pp.1-15
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• 2006

This study examines the social meaning of the CosPlay, the growth potential of CosPlay culture and its effect on the related industry through the perspectives and language of the youths who enjoy CosPlay, based on the ethnographic research. Also, this study presents a comparative description of Korea and Japan CosPlay culture by the ethnographic methodology whose purpose is to define relationship of cause and effect with phenomenon. For further step, this study plans to emphasize the need to link culture, clothes and related industry in order to create a cultural environment where diversity co-exists. CosPlay is the mania culture of Japan that emulated the Halloween party of the West and that developed the party into a unique form. In Korea, this practice was accepted for the first time among a handful of youths, starting from the 1990s, after which, it was introduced to the masses while holding CosPlay related events. While CosPlay is succeeded as an industry in Japan, CosPlay in Korea is considered childish play due to the Korean culture of considering cartoon as a childish and low class genre which is enjoyed by youths. CosPlay in Korea faces the following changes: aging of the members who comprised the CosPlay culture at the initial stage; population increase, centered on middle and high school students; interest of the government and the businesses that wish to produce economic wealth by organizing CosPlay events into events for youths; and changes in the environment that comprised the surrounding of the CosPlay culture. CosPlay is an honest play that demonstrates one's effort on the stage through performance. Moreover, most of the middle and high school students who comprise the CosPlay culture demonstrate similar characteristics as mania type of people when it came to the reason that they enjoy CosPlay. However, they did not consider CosPlay culture as an important aspect of their lives. Instead, most of them said that they participate to relieve stress. Thus, they have the potential to move onto another form of youth culture that may appear more attractive to them. To them, it is not the CosPlay culture that is important, but the fact that CosPlay provides a forum where they can freely engage in play.

• Journal of the Korean Chemical Society
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• v.53 no.2
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• pp.175-188
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• 2009

In this study, we developed an experimental method of the Charles' law applicable to school. Science textbooks and literatures on this principle were analyzed to extract factors utilized in organizing the experimental setup and method. A combined structure such as with a vial and a glass tube, the former of which is for deciding the total volume and the latter of which is for easy measurement of volume, was better in measurement of volume with temperature rather than a simple structure such as syringe. Use of graduated cylinder as a water bath to control the temperature showed advantage in cooling time than using other bath of larger volume such as a beaker. A liquid drop was used as a plug in the glass tube. This plug has little resistance with the glass wall when the gas volume changes. Water as a liquid drop in the glass tube had a significant effect in volume change of gas due to evaporation, especially in the beginning of the measurement. Glycerol showing negligible effect in volume change was used. This method took about one hour and produced a good linear relationship between the temperature and volume of gas with $R^2$ = 0.999 and absolute zero temperature = $-216.7\;{^{\circ}C}$. The Charles' law experiment developed in this study can be performed with appropriate adjustment of procedure considering the purpose of the curriculum of science and chemistry subject at each school level.

• Journal of Technologic Dentistry
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• v.36 no.4
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• pp.277-296
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• 2014

Purpose: This study intends to set standard for organizing the curriculum of short-term dental technicians and the change of job environment, as well as the knowledge & training, by identifying the importance and actual job performance of dental technicians following job analysis in the university education of Dept. of Dental Laboratory Technology, and furthermore to foster junior executives to cope with modernization and globalization. Methods: The tools used in this study are based on the job analysis of research report suggested by National Health Personnel Licensing Examination Board(Lee Gyu-seon, 2011), and it consists of general characteristic 7 items. Every competence duty was measured through Likert 5 point gauging, and internal consistency through Cronbach's Alpha. The competence importance of entire questionnaire was .984 and its performance was .874, reflecting considerably high level, and in terms of each competence duty, high credibility was proved and high internal consistency was verified with competence importance ranging from the minimum .655 to the maximum .966 and its performance ranging from the minimum .677 to the maximum .993. Results: Competence importance and its performance in each duty on the job analysis of dental technicians were measured. In competence importance in each duty, the highest was "checking design order form"(average, 4.52), followed by "selling dental prosthesis"(average 4.49), "making removable orthodontic appliance"(average, 4.48), "open managing dental laboratory"(average, 4.46) in order. However, "making complete denture" was found the lowest(average, 4.23), with importance of all competence dutys was considerably high level. In the performance of its competence duty, "checking design order form"(average, 4.04) was found to be the most importance competence, followed by "making conservative restoration"(average, 4.00), "making porcelain"(average 3.98), "checking working cast"(average, 3.90) in order. However it was found out that "making fixed orthodontic appliance"(average, 3.12) was the lowest, and the importance of all competence dutys was quite high level. Conclusion: The duties of dental technicians consist of 13 kinds, in total, and it was found out that "checking design order form"(average, 4.52) was the most important in the competence importance and job performance. In particular, it was revealed that there was a great difference between the competence importance and its performance in the order of "selling dental prosthesis", "open managing dental laboratory", "making removable orthodontic appliance", "making CAD/CAM prosthesis", and then "making fixed orthodontic appliance".

• Journal of The Korean Association For Science Education
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• v.12 no.2
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• pp.109-128
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• 1992

Elementary science textbooks are the major instructional materials which contain sceientific objectives to be acquired by students. The purpose of this study is to analyze the development system, to evaluate the inquiry activity in current elementary science testbooks, and to make suggestions for the improvement of them. The questionnaires were administered to 116 subjects that participated in development of the 5th elementary science textbooks, and 86 of the subjects responded. Based on the data gathered and analyzed in this study, the major findings are as follows. First, regarding the analysis and evaluation of elementary science textbooks. 1. The instructional objectives in elementary science textbooks are analyzed. The proportions of comprehension objectives is 38%, scientific inquiry process objectives 21%, experimental skills objectives 7%, scientific attitudes and interests objectives 4%. 2. The science concepts in elementary science textbooks of the 3rd grade required the preconcrete operational level(82%) and of the 6rd grade required the postconcrete operational level (73%) by J. Piaget 3. The inquiry activities in elementary science textbooks are emphasizing gathering and organizing results and evaluation, and hypothesizing and designing an experiment Inquiry index of the elementary science textbooks is 76. This is significantly higher than 35${\sim}$50 of America and U.K.. 4. The number of questions per class hour is mostly three, and most of them require the students to explain phenomena in nature or in experiment Second, regarding the development system of elementary science textbooks. 1. The budget for and the period of the development of elementary science textbook should be expanded. 2. For the improvement of elementary science textbooks, more abundant resources and time should be used for the basic study and the field trial of textbooks. 3. The elementary science textbook must include the affective objectives as well as the scientific knowledges and scientific inquiry skills. 4. It is not desirable to assign equal number of pages to the content of physics, chemistry, biology, and earth science. 5. Closer cooperation system is needed among the ministry of education, development research center and the field trial schools.