• Title/Summary/Keyword: Organisms

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Knowledge and Compliance of the Multidrug-resistant Organisms of Nursing Students with Clinical Practice (임상실습을 경험한 간호대학생의 다제내성균에 대한 지식 정도와 감염관리 이행)

  • Sona, Lee;Hye-Seon, Choi
    • Journal of Industrial Convergence
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    • v.20 no.11
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    • pp.205-211
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    • 2022
  • This study is a descriptive research study to identify the knowledge and compliance of multidrug-resistant organisms of nursing students with clinical practice. Data were collected from 212 nursing students in two nursing schools in D city from 1 November 2020 to 10 March 2021. The collected data were statistically analyzed using the SPSS/WIN 25.0 program. Correlations were found between knowledge and compliance of multidrug-resistant organisms (r=.40, p<.001). Therefore, to enhance the compliance of multidrug-resistant organisms of nursing students, it is necessary to prepare detailed theoretical and practical guidelines for multidrug-resistant organisms in the curriculum and clinical practice and to increase knowledge through multidrug-resistant organisms infection control education.

Removal of Chromium (VI) by Escherichia coli Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study

  • Zhou, Xiaofeng;Li, Jianghui;Wang, Weilong;Yang, Fan;Fan, Bingqian;Zhang, Chenlu;Ren, Xiaojun;Liang, Feng;Cheng, Rong;Jiang, Fengying;Zhou, Huaibin;Yang, Juanjuan;Tan, Guoqiang;Lyu, Jianxin;Wang, Wu
    • Journal of Microbiology and Biotechnology
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    • v.30 no.7
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    • pp.996-1004
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    • 2020
  • Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorption-desorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

An Experimental Study on Water-Purification Properties in Cement Bricks Using Effective Micro-Organisms and Zeolite (유용 미생물과 제올라이트를 이용한 시멘트 벽돌의 수질 정화 특성에 관한 실험적 연구)

  • Kim, Wha-Jung;Choi, Kil-Jun;Park, Jun-Seok
    • Journal of the Korea Concrete Institute
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    • v.23 no.3
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    • pp.331-338
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    • 2011
  • The purpose of this study is to use organisms or micro-organism functions for eco-friendly water-purification of cement bricks, utilizing bioremediation. Many researches have been performed in the past to improve water quality by using effective micro-organisms in construction materials. In order to purify water using micro-organisms, this research used soybean paste bacteria, an effective micro-organism that was identified through 16S rDNA sequence analysis performed in Daegu S. Environment Protection Institute in addition to Natto bacteria that was studied in the previous research. With these effective micro-organisms with water-purification ability, this study examined their water-purification possibility on cement bricks. This study used Zeolite to immobilize micro-organisms to bricks, and confirmed that the micro-organisms were attached on Zeolite from SEM analysis. The experimental results showed that specific micro-organisms can be used to effectively remove contamination an used to develop eco-friendly construction materials. The study on micro-organisms for material purification shows great promises as a future research topic.

Symbiotic organisms search algorithm based solution to optimize both real power loss and voltage stability limit of an electrical energy system

  • Pagidi, Balachennaiah;Munagala, Suryakalavathi;Palukuru, Nagendra
    • Advances in Energy Research
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    • v.4 no.4
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    • pp.255-274
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    • 2016
  • This paper presents a novel symbiotic organisms search (SOS) algorithm to optimize both real power loss (RPL) and voltage stability limit (VSL) of a transmission network by controlling the variables such as unified power flow controller (UPFC) location, UPFC series injected voltage magnitude and phase angle and transformer taps simultaneously. Mathematically, this issue can be formulated as nonlinear equality and inequality constrained multi objective, multi variable optimization problem with a fitness function integrating both RPL and VSL. The symbiotic organisms search (SOS) algorithm is a nature inspired optimization method based on the biological interactions between the organisms in ecosystem. The advantage of SOS algorithm is that it requires a few control parameters compared to other meta-heuristic algorithms. The proposed SOS algorithm is applied for solving optimum control variables for both single objective and multi-objective optimization problems and tested on New England 39 bus test system. In the single objective optimization problem only RPL minimization is considered. The simulation results of the proposed algorithm have been compared with the results of the algorithms like interior point successive linear programming (IPSLP) and bacteria foraging algorithm (BFA) reported in the literature. The comparison results confirm the efficacy and superiority of the proposed method in optimizing both single and multi objective problems.

Perceptions and Image Analysis of Elementary Students on Scientists studying Small Organisms (작은 생물을 연구하는 과학자에 대한 초등학생들의 인식 및 이미지 분석)

  • Choi, Youngmi;Hong, Seung-Ho
    • Journal of Korean Elementary Science Education
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    • v.33 no.4
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    • pp.655-673
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    • 2014
  • We investigated perceptions and image analysis on scientists studying small organisms reflected in elementary student's drawing using a modified version of the Drawing-A-Scientist-Test. The participants were 530 of fifth and sixth graders consisted of 449 ordinary students and 81 science gifted students. The data were collected from associated words, images and explanatory notes depicted by students engaged in questionnaires. The results indicated that a larger number of students reminded small sized animals and/or plants as words associated with small organisms. In addition, some students depicted anthropomorphic or abstract microorganisms. In this study, more stereotypes of scientists' appearance were exhibited at sixth graders and city region group. Most of the students depicted indicators such as lab coat, glasses, scientific instruments for observing, indoor, male and young, whereas only a few students depicted collaborative work. There was statistically significant difference between girls and boys, because boys perceived male scientists only, while half of girls depicted female. More frequent research instruments and scientific captions were used when science gifted students depicted scientists studying small organisms. These results could be contributed to education on microorganisms in elementary science.

Microorganism lipid droplets and biofuel development

  • Liu, Yingmei;Zhang, Congyan;Shen, Xipeng;Zhang, Xuelin;Cichello, Simon;Guan, Hongbin;Liu, Pingsheng
    • BMB Reports
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    • v.46 no.12
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    • pp.575-581
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    • 2013
  • Lipid droplet (LD) is a cellular organelle that stores neutral lipids as a source of energy and carbon. However, recent research has emerged that the organelle is involved in lipid synthesis, transportation, and metabolism, as well as mediating cellular protein storage and degradation. With the exception of multi-cellular organisms, some unicellular microorganisms have been observed to contain LDs. The organelle has been isolated and characterized from numerous organisms. Triacylglycerol (TAG) accumulation in LDs can be in excess of 50% of the dry weight in some microorganisms, and a maximum of 87% in some instances. These microorganisms include eukaryotes such as yeast and green algae as well as prokaryotes such as bacteria. Some organisms obtain carbon from $CO_2$ via photosynthesis, while the majority utilizes carbon from various types of biomass. Therefore, high TAG content generated by utilizing waste or cheap biomass, coupled with an efficient conversion rate, present these organisms as bio-tech 'factories' to produce biodiesel. This review summarizes LD research in these organisms and provides useful information for further LD biological research and microorganism biodiesel development.

NEW RECOGNITION AND IDENTIFICATION MERHOD FOR MICRO-ORGANISMS BY EXPERT SYSTEM DRIVEN IMAGE PROCESSING

  • Fukuda, Toshio;Hasegawa, Osamu
    • 제어로봇시스템학회:학술대회논문집
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    • 1989.10a
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    • pp.1005-1010
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    • 1989
  • A refined version of automatic micro-organism recognition and identification method, 'O.I.S.M.2' is proposed in this paper, using image processing based on an expert system. This proposed method is based on the segmentation of the organism image, characterizing segment features, which are independent of individual size and length. Complicated shapes of organisms are divided into basic shape segments defined in this paper such as lines, circles, ovals etc. Organisms can then be expressed simply in a set of segments, regardless their individual differences.

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Anti-inflammatory Compounds from New Zealand Marine Organisms

  • Webb, Victoria L.;Pearce, A. Norrie;Maas, Elizabeth W.
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.3
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    • pp.137-143
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    • 2006
  • The market for anti-inflammatory drugs is large and is expanding rapidly as populations age. Key to the development of new drugs are novel chemotypes. Marine organisms harbour a diverse range of unique compounds with applications in a multitude of disease indications. This review looks at anti-inflammatory compounds isolated from New Zealand marine organisms.

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Food Organisms of Fingerlings of Acentrogobius elongata Inhabited at Intertidal Zone of the Western Coast of Korea

  • Kim, Jong-Yeon;Jo, Soo-Gun;Yoon, Jong-Man
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.93-94
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    • 2003
  • To investigate the food organisms of the Acentrogobius elongata during the juveniles stage, the stomach contents of fish, captured in the intertidal zone of Chungchongnam-do Sochon-gun Su-myon Dodun-ri between on early in June from the end of May 2000, were observed. Total length of the fingerings of A. elongata was 1.0~3.0cm size, and the participation rate of feeding was 68.6%. Main food organisms were such as copepods, shrimp larvae, polychaete larvae, and these occupied more than dry weight 4%. Copepods among them dominated the most quantitys by average 64.6%, and food organismsms appeared by order of polychaete larvae and shrimp larvae etc.. Therefore, most important food organisms of fingerlings stage of A. elongata were copepods, polychaete larvae, shrimp larvae etc.

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Food Organisms of Juveniles of Tridentiger trigonocephalus Inhabited at Intertidal Zone of the Western Coast of Korea

  • Kim, Jong-Yeon;Jo, Soo-Gun;Yoon, Jong-Man
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2002.05a
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    • pp.359-360
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    • 2002
  • To investigate the food organisms of the Tridentiger trigonocephalus during the juveniles stage, the stomach contents of fish, captured in the intertidal zone of Chungchongnam-do Sochon-gun Su-myon Dodun-ri between on early June from end if may 1999, were observed. Total length of the juveniles of T. trigonocephalus was 5.5mm∼9.0mm size, and the participation rate of feeding was 68.5%. Main food organisms were such as copepods, amphipods, shrimp larvae, polychaete larvae, and these occupied more than dry weight 2%. Copepods among them dominated the most quantitys by average 67.5%, and next, food organismsms appeared much by order of polychaete larvae and shrimp larvae etc.. Therefore, most important food organisms of juvenile stage of T. trigonocephalus were copepods, polychaete larvae, shrimp larvae etc.

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