• Kosin Medical Journal
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• v.33 no.2
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• pp.159-170
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• 2018

Objectives: The purpose of this study was to compare antibiotic resistance patterns between first urinary tract infection (UTI) and recurrent UTI groups and to obtain information regarding empirical antibiotic selection for treating recurrent UTI. Methods: We retrospectively reviewed 148 children treated for UTIs from January 2009 to June 2016. The patients were divided into two groups: first UTI (N = 148) and recurrent UTI (17 patients and 20 episodes). Results: In both groups, Escherichia coli was the most frequent causative organism, accounting for 89.9% and 75.0% in the first and recurrent UTI groups, respectively. When E. coli or Klebsiella pneumoniae was the causative organism, extended-spectrum ${\beta}-lactamase$ (ESBL)-producing organisms were more frequent in the recurrent UTI group (17.6%) than in the first UTI group (14.0%); however, this difference was not statistically significant (P = 0.684). Cefotaxime was the most frequently used first-line empirical antibiotic in both groups. In the first UTI and recurrent UTI groups, 7.4% and 15.0% of patients were treated with intravenous antibiotics as definitive therapy, respectively (P = 0.250). Fifteen out of 17 patients having a second UTI had different causative organisms or antibiotic susceptibility patterns compared to their previous episode. Conclusions: Escherichia coli was the most frequent causative organism in the recurrent UTI group. There were no differences in the proportion of ESBL-producing organisms between the first UTI and recurrent UTI groups. Therefore, when a UTI recurs in children, the antibiotics effective on the most common causative organism might be administered as empirical antibiotics.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.51-57
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• 1990

In order to know the prevalence of S. hyicus subsp hyicus in pigs, attempts were made to isolate the organism from 549 healthy adult pigs, 277 healthy suckling piglets and 17 piglets with exudative epidermitis from April 1988 to January 1989 in Chinju, Korea. Also determined was antibiotic susceptibility of the isolates. Isolation rates of S. hyicus subsp. hyicus from adult pigs and suekling piglets were 27.0% and 53.1%, respectively. The organism was isolated predominantly from abdomen (10.9%) of adult pigs and external ear (31.6%) of suckling piglets. Isolation rates of the organism from piglets of different age group were in order of prevalence of 1(82.1%), 3(74.0%), 2(54.7%), 4(52.9%), 5(15.2%) and 6(5.7%) post-natal weeks. All of 489 isolates were sensitive to gentamicin, kanamycin and cephalexin but 23.7~79.6% of the strains were resistant to erythromycin, penicillin G and tetracycline. The 49.2% of these resistant strains exhibited multiple drug resistance. The drug resistant patterns most frequently encountered were PG TC EM(10.7%) in triple pattern, PG TC(23.0%) in double pattern and TC(44.4%) in single pattern.

• Microbiology and Biotechnology Letters
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• v.19 no.1
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• pp.8-13
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• 1991

Bacteriocin-producing microorganisms were screened from raw milk and tested their antimicrobial activities against Lactobacillus plantarum ATCC 8014 as target organism, Antimicrobial substances isolated showed broad antimicrobial spectra against Gram positives and negatives. Strain 1112-1 was selected as a test organism due to its highest antimicrobial activity among the isolates. Antimicrobial substance produced by 1112-1 completely suppressed the growth of Lactobacillus plantarum at 230 IUIml and showed 11% growth inhibition of E. coli at 500 IUIrnl level. The antimicrobial substance was found to be proteinaceous material which was inactivated by carboxypeptidase, elastase, alpha amylase, amyloglucosidase, pronase, protease IV, alpha chymotrypsin, ficin, cellulase, phosphatase and lipase. The molecular weight was estimated by SDS-PAGE as 5,900. The isolate 1112-1 was identified as one of the related strains of Lactococcus sp. The strain was different from Lactococcus lactis in the following characteristics: late positive in maltose and sucrose fermentation; positive in mannitol and salicin fermentation; negative in lactose fermentation.

• Journal of the Korean Home Economics Association
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• v.45 no.7
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• pp.71-91
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• 2007

We investigated the ecological variables of adolescent sexual behavior. We grouped the behaviors into organism, microsystem, mesosystem, and exosystem. The variables belonging to the organism group were sexual attitude, sex, grade, sexual knowledge, sexual education, dating experience, problem behaviors, and self-control. The microsystem variables included the parent-adolescent communication about sexuality, parental monitoring, parent educational background, friend relationships, and school environment. Moreover, the mesosystem variables included family-peer and family-school relationships. The exosystem variables were comprised of neighborhood environment and pornography. The study group included 369 adolescents from the first and second grade of several high school in Daegu, Korea. We found that the factors affecting adolescent sexual behavior included grade, sex, dating experience, self-control, smoking, and pursuing sexual pleasure. As well, contact with deviant friends, father-adolescent communication about sexuality, school type, and attachment to teacher. In addition the family-peer relationship also affected adolescent's sexual behaviors as well as pornography and neighborhood environment. Lastly the variable belonging to the organism group was found to have the greatest effect on adolescent, sexual behavior compared to the other variables.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2006.06a
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• pp.149-150
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• 2006

Displayed result that handle particle contaminant and hydrospace organism included in number of ballast that is happened in ship using automatic back washing filter. Reason that remove first contaminant that is included in number of ballast is that heighten processing effect of after processing process. of the filter. Another advantage is to drop oft the solids with controlling revolution of drum screen in pretreatment filtration process. The fact that it is easy to attach and detach a several type of screen for getting the expected water quality is another advantage. Filter rotation speed at 1.0rpm is filter resistance 3.0bar and 3.0rpm is filter resistance 2.8bar. Filter out impurities from ballast water over 6.0rpm is filter resistance 2.6bar and 10rpm is filter resistance 2.5bar. Filtration system removal aquatic organism over $80{\mu}m$ in ballast water. This study shows that the filtration treatment system has a potential for the treatment of ballast water.

• Proceedings of the Korea Contents Association Conference
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• 2005.11a
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• pp.257-263
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• 2005

If baby disease not treats at early, case that cause problem in health hereafter much exists. Specially, because baby expresses own inexpedience by crying, parents or practitioner's is not apt to grasp. Therefore, In this paper, I wish to develop system for analyze if disease is in some region to analyze face and baby cry that organism signal appears intensive for baby disease diagnosis. In this paper, I wish to propose methodology that analyze organism signal that appear in face of whole baby organism signal analysis system. For this, I necessary region binary input baby face picture and to draw necessary five sensory organ and Myungdang region in baby disease diagnosis. Finally, the usefulness of this paper is verified by several experiments.

• Natural Product Sciences
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• v.18 no.2
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• pp.102-105
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• 2012

Marine organism extracts were prepared from 26 species of Korean indigenous marine organisms, including 25 species belonging in class Anthozoa of phylum Cnidaria and a species belonging to subphylum Urochordata of phylum Chordata, and screened their inhibitory effects against ${\mu}$-calpain. As a result, the thirteen extracts were found to be active in the criteria of $IC_{50}$ < 100 ${\mu}g/ml$. Among them, the MeOH extracts of Plexauroides praelonga and Alveopora japonica showed remarkable ${\mu}$-calpain inhibitory activity with $IC_{50}$ values of $4.62{\pm}0.22$ and $4.82{\pm}0.07{\mu}g/ml$, respectively. In addition, chemical investigation of A. japonica led to the isolation of an active compound, hexadecyl tetradecanoate, as a selective cathepsin B inhibitor ($IC_{50}=9.05{\pm}2.45{\mu}M$). This compound was isolated as constituent of A. japonica for the first time in the present study.

• Korean Journal of Veterinary Service
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• v.24 no.1
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• pp.43-50
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• 2001

Swine proliferative enteritis(SPE) caused by inかsoma intracellularis is a common enteric disaese of grower and finisher pig. Swine affected with SPE show variable clinical signs including diarrhea, weight loss, aberrant growth and death. The characteristic lesion of ileitis at necropsy is marked thickening of the last section of the small intestine. The inner lining of the thickened intestine proliferates almost like a cancer and curved rod bacteria(L intracellularis) are always seen inside the intestinal wall. Infected swine shed the organism in the feces. Isolation and growth of pure L intracellularis in vitro requires a suitable cell culture. This procedure is difficult and not a practical means of diagnosis, thus the polymerase chain reaction(PCR) test of feces can be used to determine whether a pig is shedding the infective organism. A sensitive assay based on amplification of a 319bp DffA fragment of the L intracellularis of Swine proliferative enteritis was attempted for the detection of the organism in the 62 feces of swine. L intracellularis was identified on three herds and detected in 6 fecal samples, representing a infection rate of 9.7%. The PCR was very sensitive and specific on the individual level. The PCR technique could be very useful for the diagnosis of this disease.

• Korean Journal of Veterinary Service
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• v.30 no.2
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• pp.251-258
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• 2007

Bovine brucellosis has occurred for years in Gyeonggi province under the national test and slaughter scheme. The serum agglutination test (SAT) is a diagnostic tool to confirm the disease despite the argument on its specificity. We selected 8 farms where only one or two individuals were diagnosed as brucellosis through SAT at the primary regular herd check and isolated the causative organism and characterized the species by species-specific PCR. The pathogen isolation was successful in 6 farms out of 8 farms by microbiological culture, showing the successful rate of 75%. The isolation rate of the causative organism represents 70% from supra-mammary lymph node and 60% from uterine tissues. They were characterized as Brucella abortus biovar 1 after biotyping by PCR, showing the fragment of 498 bp. Five of 8 farms were diagnosed as brucellosis two to four times more over the intervals of two or three months. Here in this study we briefly showed the correlation of the sporadic outbreak of brucellosis tested by SAT and the isolation of the causative organism. Moreover one or two reactors against brucellosis among considerable size of herd may indicate that SAT failed to detect potentially infected individuals in the incubation stage or chronic phase of the disease.

• Journal of Microbiology
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• v.42 no.2
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• pp.69-73
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• 2004

Vibrio vulnificus, a Gram-negative bacterium found in estuarine waters, is responsible for over 95％ of all seafood-related deaths in the United States. As a result of a temperature downshift to 5$^{\circ}C$, this organism enters the viable but nonculturable (VBNC) state. Changes in the membrane fatty acid (FA) composition of V. vulnificus may be a contributing factor to the ability of this organism to enter into and survive in the VBNC state. This hypothesis was tested by incubating the organism at 5$^{\circ}C$ in arti-ficial sea water and analyzing the cells' FAs during the initial hours of temperature and nutrient down-shift. Prior to downshift, the predominant FAs were 16:0, 16:1 and 18:0. During the first four hours of downshift, statistically significant changes occurred in 15:0, 16:1, 16:0, 17:0, and 18:0. These results indicate that changes in FA composition occur prior to entry of V. vulnificus into the VBNC state, suggesting that the ability to maintain membrane fluidity may be a factor in this physiological response. Cells in which fatty acid synthesis was inhibited did not survive, indicating that active fatty acid metab-olism is essential for entry of cells into the VBNC state.