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Evaluation of ${\mu}$-Calpain Inhibitory Activity of Korean Indigenous Marine Organism Extracts  

Lee, Yoo-Jin (College of Pharmacy, Ewha Womans University)
Lee, Eun-Young (College of Pharmacy, Ewha Womans University)
Han, Ah-Reum (College of Pharmacy, Ewha Womans University)
Song, Jun-Im (Department of Biological Science, Ewha Womans University)
Kwon, Young-Joo (College of Pharmacy, Ewha Womans University)
Seo, Eun-Kyoung (College of Pharmacy, Ewha Womans University)
Publication Information
Natural Product Sciences / v.18, no.2, 2012 , pp. 102-105 More about this Journal
Abstract
Marine organism extracts were prepared from 26 species of Korean indigenous marine organisms, including 25 species belonging in class Anthozoa of phylum Cnidaria and a species belonging to subphylum Urochordata of phylum Chordata, and screened their inhibitory effects against ${\mu}$-calpain. As a result, the thirteen extracts were found to be active in the criteria of $IC_{50}$ < 100 ${\mu}g/ml$. Among them, the MeOH extracts of Plexauroides praelonga and Alveopora japonica showed remarkable ${\mu}$-calpain inhibitory activity with $IC_{50}$ values of $4.62{\pm}0.22$ and $4.82{\pm}0.07{\mu}g/ml$, respectively. In addition, chemical investigation of A. japonica led to the isolation of an active compound, hexadecyl tetradecanoate, as a selective cathepsin B inhibitor ($IC_{50}=9.05{\pm}2.45{\mu}M$). This compound was isolated as constituent of A. japonica for the first time in the present study.
Keywords
Korean indigenous marine organism; coral; Alveopora japonica; hexadecyl tetradecanoate; ${\mu}$-calpaincathepsin B;
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