• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.201-205
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• 2000

Eleven runoff $plots(3{\times}15\;m)$ were built on a sloping field of a high plateau in Kangwon Province, Korea. The plots were treated with different tillage, residue covers and fertilizers, corn and potato were cultivated, and sediment discharge was measured from the plots for 3 years. Agricultural management practices were monitored around the plots to develop adequate best management practices. The least sediment occurred from the plots with no-till and 100% residue cover $(corn,\;0.1{\sim}2.2\;t/ha/year)$ and with contour tillage and vinyl sheet cover plots $(potato,\;0.1{\sim}0.2\;t/ha/year)$. The largest sediment was measured from the plots with up-and-down till and no cover ($11{\sim}33$ and $16{\sim}31\;t/ha/year$ from corn and potato plots, respectively). The type of organic and commercial fertilizers seemed not to affect sediment discharge. Sediment discharge from contour plots were largely dependent on the collapse of ridges due to the flush of water stored in furrows. The sediment discharge from contour corn and potato plots with no residue cover was $10{\sim}27$ and $16{\sim}24\;t/ha/year$, respectively. No-till with residue cover, vinyl cover for tuber crops, construction of furrow dam, frost heave research, furrow and ridge tillage with furrow dam after tuber crop harvest, limited stalk harvest of row crops, use of winter cover crops, and other common best management practices were recommended to minimize the sediment discharge from a high plateau sloping land culture.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.3
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• pp.159-163
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• 1973

A by-product from glutamic acid fermentation prepared by treating the fermentation residue with sulfuric acid and ammonium hydroxide was studied in the light of its chemical properties by chemical analysis and X-ray diffraction technique. Its effectiveness as fertilizer was also investigated with corn as test plant. The results are summarized as following. 1. The chemical analyses and study with X-ray diffraction technique revealed that the by-product contains an appreciable quantity of water soluble ammonium as ammonium sulfate and ammonium chloride, water soluble amino acid and in-soluble organic carbon. Particularly, the X-diffraction pattern of the material indicated the presence of unidentified water soluble double salt of ammonium sulfate and ammonium chloride. 2. The water soluble ammonium in this material was found to be as effective as urea on the growth of corn plant. 3. The organic portion of the material was found to be equally effective on the growth of corn plant as the other sources of organic matter such as compost and rice bran. It was, however found that the organic matter in the fermentation residue is more persisting in the soil than the compost and rice bran. 4. It was noticed that the application of the fermentation by-product, lowers the soil pH significantly. Inspite of the pH lowering effect, the absorption of iron by corn plant was surpressed by the application of this material.

• Journal of Biosystems Engineering
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• v.30 no.6 s.113
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• pp.333-339
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• 2005

The pesticide is raising public interest in the world, because it causes damage to an environmental pollution and the human health remaining agricultural products and an ecosystem, in spite of the advantages. Particularly, each country restricts the residual pesticide and induces observance about the safety and usage standard so that they can control the amount of pesticide used and defend the safety of agricultural products. The habitual practice for the analysis of the residual pesticide depends on GC (gas chromatography), HPLC (high performance liquid chromatography) and GC/MS (gas chromatography/mass spectroscopy), which triturate the fixed quantity of samples, abstract and purify as a suitable organic solvent. These methods have the highly efficient in aspects of sensitivity and accuracy. On the other hand, they need the high cost, time consuming, much effort, expensive equipment and the skillful management. Carbofuran is highly toxic by inhalation and ingestion and moderately toxic by dermal absorption. As with other carbamate compounds, it is metabolized in the liver and eventually excreted in the urine. The half-life of carbofuran on crops is about 4 days when applied to roots, and longer than 4 days if applied to the leaves. This research was conducted to develop immunoassay for detecting carbofuran residue quickly on the basis of surface plasmon resonance and to evaluate the measurement sensitivity. Gold chip used was CM5 spreaded dextran on the surface. An applied antibody to Immunoassay was GST (glutathione-s-transferase). The association and the dissociation time were 176 second and 215 second between GST and carbofuran. The total analysis time using surface plasmon resonance was 13 minutes including regeneration time, on the other hand HPLC and GC/MS was 2 hours usually. The minimum detection limit of a permissible amount for carbofuran in the country is 0.1 ppm. The immunoassay method using surface plasmon resonance was 0.002 ppm.

• Journal of Environmental Impact Assessment
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• v.22 no.6
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• pp.547-556
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• 2013

Recently, golf courses have increased over the years because golf became popular leisure sport. Various environmental problems have been then issued by a golf course during constructing and running them. A problem of pesticide, which is serious among various environmental problems, from golf course has harmful effect on surrounding area and makes human suffer from acute and chronic diseases. Pesticides are used for the cost-effective managing of golf course and the amount of pesticides also increases as the number of golf course increase. Since the assessment of pesticides on near-by surrounding has been focused on water and soil media, studies related to atmospheric dispersion have been hardly attempted. The method to assess an impact of pesticide nearby agricultural production by the atmospheric dispersion using AGDISP(AGricultural DISPersal) model was developed and applied to the actual planned golf course located in Hongcheon, Gangwon. For implementing AGDISP, parameters were investigated from the golf course's land use planning map, pesticide spray device, Hong-Cheon weather station and etc. First of all, a kind of pesticide, a form of spraying pesticide, geographical features, weather data, and distance(golf course to plantation) were investigated to understand how to work these parameters in AGDISP. Restricted data(slope angle, droplet size distribution and solar insolation) sensitivity analysis of these parameters to estimate effect of pesticide nearby a plantation and a high relative contribution data of analyzed data was selected for input data. Ethoprophos was chosen as the pesticide used in the golf course and the amounts of pesticide deposition per annual agricultural productions were predicted. The results show that maximum amount of pesticide deposition through atmospheric dispersion was predicted $2.32{\mu}/m^2$ at 96 m where the nearest organic plantation exists. The residues of pesticide were also estimated based on the annul production of the organic and the deposition amount of the pesticide. Consequently, buckwheat, wheat and millet were likely to exceed maximum residue limits for pesticides in foods(MRL) and sorghum, corn and peanut were likely to exceed MRL by organic farming as well.

• Journal of Life Science
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• v.32 no.7
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• pp.501-509
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• 2022

Pollen corrected from Quercus species is abundant in Korea and has been used to treat anemia and inflammation of the prostate gland. It is also used for hemostasis in oriental medicine. In this study, the ethanol extract of the acorn pollen and its subsequent organic solvent fractions using hexane, ethylacetate, butanol, and water residue were prepared, and their antioxidant, anti-thrombosis, and hemolysis activities were evaluated. The ethylacetate fraction of acorn pollen (EF-AP) showed the highest polyphenol content (225.0 mg/g) and strong antioxidant activity among the solvent fractions. The RC₅₀ of EF-AP against DPPH, ABTS, and nitrite radicals was 72.2, 27.7, and 62.6 ㎍/ml, respectively. The blood coagulation activities of the all-solvent fractions determined by thrombin time, prothrombin time, and activated partial thromboplastin time were negligible up to 5 mg/ml. Platelet aggregation inhibitory activities were observed in the EF-AP, butanol fraction, and water residue, whereas hexane fraction induced strong platelet aggregation. The EF-AP has no hemolysis activity against human RBC up to 1 mg/ml. UPLC/MS/MS analysis of the EF-AP revealed that rutin, isoquercitrin, and astragalin are major compounds for antioxidant and anti-thrombosis activities. Our results suggest that EF-AP could be developed as a noble antioxidant and anti-thrombosis agent.

• Korean Journal of Environmental Agriculture
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• v.8 no.1
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• pp.7-16
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• 1989

Application of steam distillation on the analysis of pesticide residue in soil, water and crops was studied using the nitrogen distillation apparatus. Most pesticides which were extracted by organic solvents could be analyzed by the steam distillation method. For instance, distillations of PCNB, ${\gamma}-BHC$, ${\alpha}-or$ ${\beta}-endosulfan$, IBP, diazinon, fenthion, fenitrothion, alachlor, butachlor, pretilachlor, metolachlor, pendimethalin, benthiocarb and molinate were possible, but not simazine, atrazine and nitrofen. The optimum volume of distillate for a sufficient extraction of pesticide varied according to kind of pesticide. In general, the volume needed was little for carbamate, but much more for organochlorine. When the definite amount of distillate was obtained and then the condenser was washed by acetone, the optimum volume of distillate was less. Using the steam distillation method, the amount of organic solvent needed for one extraction of pesticide from soil, water and vegetable was less than the conventional solvent extraction method, and the analytical procedure became simpler. The process of concentration and clean up was mostly unnecessary, although a ghost peak was apparent in the gas chromatogram according to the kind of materials distilled.

• Korean Journal of Clinical Pharmacy
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• v.15 no.1
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• pp.46-49
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• 2005

A simple HPLC method was employed for the determination of pentoxifylline in human serum. After addition of internal standard (IS, 50 uL of 3 ug/mL chloramphenicol methanol solution) into the serum sample, the drug and IS were extracted by dichloromethane. Following a 1-min vortex-mixing and a 15-min centrifugation at 3500 게m, the organic phase was transferred and evaporated to dryness under a vacuum. The residue was reconstituted with 120 ${\mu}L$ of mobile phase and 50 ${\mu}L$ was injected into C18 column with a mobile phase composed of 0.034 M phosphoric acid adjusted to pH 4 with 10 M NaOH and acetonitrile (75:25, v/v). The samples were detected using an ultraviolet detector at 273 nm. The method was simple, specific and validated with a limit of 10 ng/mL. Intra- and inter-day precision and accuracy were acceptable for all quality control samples including the lower limit of quantification. The applicability of this method was evaluated by analysis of human serum after oral administration of a single 400 mg dose to 8 healthy subjects. The pharmacokinetic parameters for pentoxifylline in human subjects were calculated using WinNonlin program. As a result, $AUC_{t},\;C_{max},\;T_{max}$ and $t_{1/2}$ were $962.28{\pm}645.69\;ng{\cdot}/mL$, $132.82{\pm}42.05$ ng/mL, $2.06{\pm}2.68$ hr and $8.74{\pm}4.38$ hr, respectively. Based on the results, this validated method appears to be useful fur the pharmnacokinetic study of pentoxifylline in humans.

• Analytical Science and Technology
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• v.12 no.2
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• pp.151-158
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• 1999

This study was conducted to measure the residual PCBs in sediments of 4 great rivers in Korea by gas chromatography-mass spectrometry. From the results, PCBs were positively detected in all samples. The residue levels of total PCBs in sediments in near of SS and OS were $290.87{\mu}g/kg$ and $221.11{\mu}g/kg$, respectively. The PCBs contamination may be in association with total organic compound (TOC) in sediments. Congener 66, 74, 90, 101, 105, 110, 118, 138, 149, 153, 180, 187 as IUPAC No were predominant species found in sediments and the polyCBs detected most aboundantly in sediments were tetraCBs and pentaCBs. From the study, it appears that the PCBs contamination in sediments of 4 great rivers is not serious and the analysis from enough many sites is needed for the next survey.

• Bulletin of the Korean Chemical Society
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• v.30 no.11
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• pp.2535-2541
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• 2009

Prebiotic isomaltooligosaccharide preparations contain $\alpha$-D-glucooligosaccharides comprising isomaltooligosaccharides (IMOs) and non-prebiotic maltooligosaccharides (MOs). They are both glucose oligosaccharides characterized by their degree of polymerization (DP) value (from 2 to $\sim$10), linkages types and positions (IMOs: $\alpha$-(1$\rightarrow$2, 3, 6 and in a lower proportion internal 1$\rightarrow$4) linkages, MOs: α-(1$\rightarrow$4) linkages). Their structure is the key factor for their prebiotic potential. In order to determine and elucidate the exact structure of unknown IMOs and MOs, unambiguous assignments of $^{13}C$ and $^1H$ chemical shifts of commercial standards, representative of IMOs and MOs diversity, have been determined using optimized standard one and two-dimensional experiments such as $^1H$ NMR, $^{13}C$ NMR, APT and ${^1}H-{^1}H$ COSY, TOCSY, NOESY and <$^1H-{^{13}}C$ heteronuclear HSQC, HSQC-TOCSY, and HMBC. Here we point out the differential effect of substitution by a glucose residue at different positions on chemical shifts of anomeric as well as ring carbons together with the effect of the reducing end configuration for low DP oligosaccharides and diasteroisotopic effect for H-6 protons. From this study, structural $^{13}C$ specific spectral features can be identified as tools for structural analysis of isomaltooligosaccharides.

• Applied Biological Chemistry
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• v.17 no.3
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• pp.177-183
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• 1974

One hundred and twenty-five samples comprising thirteen vegetables harvested in 1971 were collected and subjected to GLC analysis, for ${\alpha}-and\;{\gamma}-BHC$ residues, using ECD. Heavy dependence on the technical BHC, instead of Lindane, was amply reflected on the BHC residues-residues of ${\alpha}-BHC$, depending on the vegetables, amounted to $2{\sim}7$ times that of ${\gamma}-BHC$. In spite of extensive contamination by the BHC isomers, residue levels of ${\gamma}-BHC$ were less than 0.015 ppm in all vegetables and were far less than the tolerence limit of $0{\sim}3.0ppm$ recommended jointly by the FAO and WHO.