• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1665-1672
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• 2012

An 80% ethanol extract of Hizikia fusiforme was obtained and followed by successive fractionation using the organic solvents n-hexane, ethyl acetate, and n-butanol to identify the antioxidative substance. The aqueous part of the nbutanol fractionation step, showing high antioxidative activity, was subjected to reverse-phase liquid chromatography. As a result, a substance purified from a BB-2 fraction showed high antioxidative activity. The m/z 419 [M+H] molecular ion peak in the fraction was observed by the analysis of the ESI-LC/MS spectrum. By the analysis of 1H NMR (500 MHz, DMSO-$d_6$) and $^{13}C$ NMR (125 MHz, DMSO-$d_6$) spectra, a unique compound of the fraction was biochemically identified as a 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5HHMF). We also investigated the effect of 5HHMF on human gastric AGS carcinoma cells. Western blot analysis suggested that the flavone substantially increased the levels of the death receptor-associated apoptosis mediators Fas, Fas L, FADD, TRADD, and DR4 in a concentration-dependent manner. The levels of Fas, Fas L, TRADD, and DR4 in the cells treated with 5HHMF ($5{\mu}g/ml$) were approximately 26.4-, 12.8-, 6.7-, and 9.8-times higher than those of non-treated cells, respectively. Of note, the level of FADD protein in the cells exposed to 5HHMF ($1{\mu}g/ml$) increased approximately 9.6-times. In addition, the cleavage of caspase-3, -8, and -9 in cultured AGS cells treated with 5HHMF was significantly confirmed. Therefore, our results suggest that 5HHMF from H. fusiforme is involved in the induction of death receptor-associated apoptosis mediators in human gastric AGS carcinoma cells.

• Journal of Wetlands Research
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• v.15 no.3
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• pp.387-396
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• 2013

Non-point pollution source is difficult to control due to uncertain outflow path and emission. So, There are many development and research to Best Management Practices(BMP) established to manage the Non-point pollution source. Besides, various methods of estimated efficiency to exact assessment of BMP is presented. In this study, the impact about length of Grassed Swale on reduction efficiency based on monitoring results of Grassed Swale by length is studied. By estimating Grassed Swale reduction efficiency in a variety of methods, the difference between the methods of estimated efficiency was compared with those that. Estimated efficiency method using ER, SOL, ROL, ROF, SOLF, and ROLF methods is analyzed. EMC analysis result is high inflow and outflow concentration distinction organic compound for nutritive salts The result of efficiency analysis along Grassed Swale length sharply increases in a Grassed Swale inlet. After this increase, the efficiency gradually decreases. This is expected that cistern installed in the end of the front. To obtain a stable reduction efficiency of Grassed Swale, minimum length 30m of Grassed Swale should be enough. Also, in order to efficiently and economically design Grassed Swale, the researches on length of Grassed Swale are needed rather than simple analysis of efficiency.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.1058-1065
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• 2017

Amino acid based surfactants as protein-surfactants is eco-friendly compound. So, amino acid based surfactants is expected as next generation surfactants. Amino acid based surfactants has high biodegradability, low toxicity and surface active properties. In this experiment, amino acid based surfactants, cocoyl glycine, was synthesized by glycine and triglyceride such as coconut oil and palm oil. And it was tested the surface tension, emulsifying properties, foam stability and HLB value. The synthesized surfactants was confirmed by FT-IR. Surface tension of surfactants synthesized by coconut oil on diluted aqueous solutions of surfactants was 31.2 dyne/cm at $1.0{\times}10^{-4}mol/L$. Surface tension of surfactants synthesized by palm oil on the diluted aqueous solutions of surfactants was 42.1 dyne/cm at $3.2{\times}10^{-5}mol/L$. Foam stability measured the foam height as time passed. Initial foam height of surfactants synthesized by coconut oil is 14.5 cm, and 10.7 cm after five minutes. Initial foam height of surfactants synthesized by palm oil is 3.0 cm, and 2.8 cm after five minutes. Foam height of surfactants synthesized by coconut oil was higher than surfactants synthesized by palm oil. But foam stability of surfactants synthesized by palm oil was better than surfactants synthesized by coconut oil. The emulsifying properties of synthesized surfactants are observed in benzene and soybean oil and emulsifying properties in organic solvent is better than in soybean oil.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.1
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• pp.71-77
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• 2010

The water fern Azolla belongs to the Azollaceae and forms a symbiotic association with a $N_2$-fixing cyanobacterium, referred to as Anabaena azollae, and this association has currently been demonstrated to have potential as a nitrogen source for rice production. Because of that, Azolla fern has been used not only as organic manure in southern China and northern Vietnam for a long time but also as food for animate creatures in the underwater and decontaminant in the water. However, the phenolic compounds and active materials of Azolla have not been examined in detail in the past studies. In the present study, anti-oxidant ability test and experiment to find a particular active material of Azolla imbricata and Azolla imbricata fraction (AIF) were performed. In anti-oxidant test such as DPPH test and lipoxygenase inhibition test, the value of test represented high activities compared with authentic sample - green tea and NDGA (nordihydroguaiaretic acid). In MMP-1 test, related to collagen protection and elasticity of skin, its inhibitory effect was measured over 75 %, and the phenolic compounds of AIF related with this activity were confirmed luteolin derivatives by using FT-IR spectroscopy, element analyzer (EA) and Liquid chromatography-MASS spectroscopy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.483-492
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• 2021

Agastachis Herba (AH) to treat anorexia and nausea and its antidiabetic efficacy was recently reported. This study examined the antioxidant activities and chemical constituents of AH and predicted the target proteins of each compound using in silico approaches. The results showed that EC50 values of AH methanol extract for DPPH and ABTS radical scavenging were 78.6 ㎍/mL and 31.0 ㎍/mL, respectively. Compared to the EC50 values of ascorbic acid (9.9 ㎍/mL, 5.2 ㎍/mL), the AH methanol extract possessed excellent antioxidant activities. Rosmarinic acid, tilianin, agastachoside, and acetin were confirmed as the major compounds of extracts by qualitative analysis performed with HPLC-PDA-MS/MS. The antidiabetic target proteins of these compounds were predicted by applying a structural similarity and inverse docking methodology using a DIA-DB server. The resulting target proteins were PPAR-γ, DPP IV, glucokinase, α-glucosidase, SGLT2, aldose reductase, and corticosteroid 11-beta-dehydrogenase, some of which have already been proven experimentally as target proteins. Therefore, the in silico methods can be considered valid. Finally, AH were extracted with various solvents to determine the optimal conditions for the extraction of active components. Methanol among organic solvents and 80% ethanol in ethanol-water mixtures were identified as the most effective solvent for the extraction.

• Clean Technology
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• v.24 no.4
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• pp.293-300
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• 2018

Sulfamethoxazole (SMX) is sulfaamide-based synthetic antibiotics, which are widely prescribed pharmaceutical compound to treat bacterial infections in both human and animals. Most of them are not completely decomposed as refractory substances. The environmental impact of pharmaceuticals as emerging contaminants has generated severe concerns. In this study, catalytic wet peroxide oxidation (CWPO) of SMX was carried out with $Cu/Al_2O_3$ catalyst and investigated the optimum reaction conditions of temperature, dosage of catalyst and concentration of $H_2O_2$ to completely decompose the SMX. It was observed that SMX was completely decomposed within 20 min using 0.79 mM $H_2O_2$ and 6 g $Cu/Al_2O_3$ catalyst at 1 atm and $40^{\circ}C$, but SMX was not fully mineralized and converted to intermediates as hydroylated-SMX, sulfanilic acid, 4-aminobenzenesulfinic acid and nitrobenzene. After that these are completely mineralized through organic acid. We proposed the decomposition reaction path ways of SMX by analyzing the behavior of these intermediates. To investigate the durability of heterogeneous catalyst, decomposition of SMX was observed by continuously recycling catalysts. When the heterogeneous catalyst of 10 wt% $Cu/Al_2O_3$ was continuously reused 5 times, decomposition of SMX was a little lowered, but the activity of catalyst was overall very stable.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.209-218
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• 2019

This study was conducted to develop a technology for environmentally friendly control of sweet-potato whitefly, Bemisia tabaci, by controlling their behavior using a push-pull strategy in a tomato greenhouse. B. tabaci was attracted the most by yellow color, light source of 520 nm, whereas it avoided the complex light treatment of 450 + 660 nm. The two natural enemies of B. tabaci, Cyrtopeltis tenuis and Orius laevigatus, were attracted the most by 520 nm light source. B. tabaci was repelled by the volatile organic compounds ocimene and carvacrol and was the most attracted by methyl isonicotinate. When buckwheat was added into the tomato greenhouse, the density of C. tenuis was maintained at about 16 times higher than when untreated for 15 days. As a result of the combined treatment of push-pull strategy, the density per trap of B. tabaci was three times lower than when no treatment was applied, and the control of this pest increased with time and reached up to 68.7%.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.113-113
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• 2019

Diallyl trisulfide (DATS) is an organic polysulfide compound found in garlic. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In this study, we examined whether DATS exerts anti-inflammatory activity and investigated the possible mechanisms. Our results indicated that DATS significantly suppressed the lipopolysaccharide (LPS)-induced release of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional and post-transcriptional levels in RAW 264.7 macrophages. DATS also down-regulated Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression, and inhibited nuclear translocation of nuclear transcription factor-kappa B (NF-${\kappa}B$) in LPS-stimulated 264.7 macrophages. Furthermore, we found that these inhibitory effects of DATS were associated with the inhibition of chemokine receptor (CXCR4) and ligand (CXCL12) expression, and reactive oxygen species generation. Overall, the present data indicated that DATS had anti-inflammatory effects on LPS-activated macrophages, possibly via inhibiting the TLR4/NF-kB and/or chemokine signaling pathways, and DATS could be a potential drug therapy for inflammation and its associated diseases.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.126-133
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• 2019

This study intended to manufacture high quality cement, such as solving the quality problem of cement which has been emerging recently, along with improving grinding efficiency. To this end, the synthesis of melamine-functional pulverizing agents and the physical properties of cement applying them were reviewed and the reaction was carried out by dividing the melamine airborne compound into three stages of polymerization using methylation, sulfonation, and acid catalyst to improve the crushing efficiency of cement clinker and the physical properties of manufactured cement. The obtained melamine type copolymer was applied to the grinding process of cement clinker. And it's grinding efficiency and compressive strength were compared with DEG(diethylene glycol) and TIPA(triisopropanol amine). When it comes to the grinding efficiency, by lowering surface energy with stable adsorption from organic polymer to cement particles, the fineness showed 4-6% up. In the meantime, the compression strength hiked 30% from its initial strength compared to the conventional DEG. At the age of 28days, the strength showed approximately 13% improvement. Therefore, it is confirmed that the overall quality has been elevated in comparison with the conventional one.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.4
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• pp.357-365
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• 2018

This study was carried out to evaluate the weight loss rates, carbon and nitrogen dynamics of wood stakes following soil amendment treatments (CLB: compound fertilizer + lime + biochar; LB: lime + biochar) in a post-fire restoration area, Ulsan Metropolitan city, southern Korea. Soil amendments in the fire-disturbed area were applied to two-times (Mar. and Jun. 2015, 2016) during the study period. Wood stakes on Mar. 2015 were buried at a top 15cm of mineral soil in two soil amendment and control treatments of Liriodendron tulipifera, Prunus yedoensis, Quercus acutissima, Pinus thunbergii plantations and an unplanted area in the post-fire restoration area. Wood stakes were collected at Oct. 2015, Mar. 2016 and Oct. 2016 to measure weight loss rates, organic carbon and nitrogen concentrations. Weight loss rates of wood stakes were not significantly affected by soil amendment treatments. However, remaining carbon of wood stakes were lowest in the control treatment (43.7%), followed by the CLB (71.3%) and the LB (71.6%) treatments. Remaining nitrogen of wood stakes was less in the control treatment (29.7%) compared with the LB treatment (52.6%). The results indicate that carbon and nitrogen mineralization of wood stakes in post-fire restoration area were delayed by soil amendment treatments.