• Title/Summary/Keyword: Organ

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Organ Culture of Ovary Isolated from Juvenile Mice (약령 마우스에서 분리한 난소의 기관배양에 관한 연구)

  • 이현주;김지철;김기동;이상호;송해범
    • Journal of Embryo Transfer
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    • v.17 no.3
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    • pp.195-201
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    • 2002
  • This study was to assess the developmental capacity of oocytes matured in vitro after 20, 15, 10, 5 and 0 days of organ culture when ovaries were isolated from juvenile mice at 0-, 5-, 10-,15- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of ovaries and nucleus maturation of oocytes. The size of ovaries decreased 35.9%, 8.7%, 1.2% and 14.4% after 20, 15, 10, 5 days of organ culture when the ovaries were isolated from 0-, 5-, 10 and 15-day old mice, respectively. After organ culture, the recovery rates, diameters of oocytes and the number of oocytes progressed from GV to MII were increased as increasing age of mice.

Histochemical findings of the tribocytic organ and tegument of Fibricola seoulensis (Fibricolu seoulensis의 tribocytie organ 및 표피의 조직화학적 관찰 소견)

  • Sun Huh;Soon-Hyung Lee;Byong-Seol Seo
    • Parasites, Hosts and Diseases
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    • v.28 no.3
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    • pp.155-160
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    • 1990
  • The tribocytic organ and tegument of Fibricola seeulensis were examined histochemically for the detection of carbohydrates, mucosubstances, amyloid, collagen and alkaline phosphatase. The surface, secretes, gland cells of the tribocytic organ, and the tegument of the worms were positive to periodic acid Schig (PAS) and PAS with diastase stain but negative to other stains. It was inferred that the tribocytic organ and tegument of F. seoulensis comprise neutral mucopolysaccharides, which may take a protective role against host enzymes. The surface and secretes of the tribocytic organ, and the tegument of the worms were also Positive to double bridge PAP for alkaline phosphatase. This fact suggests that they may play a role as both self protective and host tissue Iytic functions.

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Brain death and organ transplantation (뇌사와 심폐사 그리고 장기이식)

  • Nam, Sang-Ook
    • Clinical and Experimental Pediatrics
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    • v.52 no.8
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    • pp.856-861
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    • 2009
  • Cardiopulmonary arrest has long been accepted as an unquestionable definition of death. An advent of cardiopulmonary resuscitation and artificial ventilation along with the development of organ transplantation has prompted the emergence of the concept of brain death. The criteria for brain death are based mainly on the clinical examination of coma, apnea and total loss of brain stem function. Although organ transplantation by donor brain death has increased in Korea over recent years, there is still a substantial shortage of donor organs compared to the demand. Improvement of government policies and changes of social culture for organ donation are needed for the activation of organ transplantation by donor brain death. Pediatricians have an important role for the search of potential donors in cases of brain death and optimal medical care for successful organ transplantation.

The Effect of Brain Death Organ Donation Education on Nurses' Knowledge and Attitude (뇌사 장기기증 교육이 간호사의 뇌사 장기기증에 대한 지식과 태도에 미치는 효과)

  • You, Hye Sook
    • Journal of Korean Clinical Nursing Research
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    • v.15 no.2
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    • pp.57-65
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    • 2009
  • Purpose: This study was conducted to examine the effects of Brain Death Organ Donation Education on nurses' knowledge and attitudes. Methods: It is a quasi-experimental study using the non-equivalent control group. A total of 100 nurses participated in the study; 50 in the experimental group, and the rest in control group. Collected data were analyzed using ${\chi}^2-test$, t-test and ANCOVA by SPSS 12.0 program Results: There was a statistically significant difference in knowledge (F=13.29, p<.001), and attitude (F=4.35, p=.040) after the experimental group received Brain Death Organ Donation Education. Conclusion: The Brain Death Organ Donation Education was revealed as an effective tool in improving nurses' knowledge and attitudes toward organ donation.

Subjectivity on Organ Donation and Transplantation (장기공여와 이식에 대한 일반인의 주관적 특성)

  • 권영미;윤은자
    • Journal of Korean Academy of Nursing
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    • v.30 no.6
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    • pp.1437-1454
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    • 2000
  • This study was designed to identify the attitudes of the people on organ donation and transplantation. The purpose of this study was to provide data to help inspire organ donation, and promote registration yield so donor candidates will have more favorable recipients through Q-methodology. A Q-sample was developed through a review of the literature and interviews. Thirty-three statements made up the final Q-sample. The P-sample consisted of twenty-eight subjects, excluding chronic organic disorder. The Q-sorts by each subject were coded and analyzed with the QUNAL computer program. The results were as follows: This study discovered five different types of organ donation and transplantation of twenty- eight subjects. Type I is 'utilitarian.' The people of this type consider human life very valuable and they recognize that organ transplantation is an affirmative medicine that should be performed to extend human life. They believe that are saving others' lives by donating organs. Type II is 'sardonist.' The people of this type approve of organ transplantation usefulness, but they have no intention of participating in the program because of it may trample on human rights. Type III is 'individualist.' The people of this type consider it proper for the activation of organ transplantation by the legal system. They believe that organ donation a valuable too, but needs support through social benefits to donors. Yet, they have not intention of doing what they propose. Type IV is 'familist.' The people of this type have strong attachments to life but they think that organ donation and transplantation should be done between within a family. Type IV is disposition of family intensive consideration rather than altruistic and utilitarianism. Type V is 'deontologist.' The people of this type recognize the benefits of transplantation, but have a negative opinion of activation. They worry about ethical and social problems occurring in the development of modern medicine. They believe that death is the only natural end to life, so they have strong negative opinions of euthanasia and brain death compared to other types. They regard transplantation to be a non-human behavior, because it involves a removing organs and breaking the boundary of death. The findings of this study are only preliminary and serve as a baseline to understanding the subjectivity of individuals on organ donation and transplantation. Therefore, the subjectivity of the five types will be applied to formulate the educational programs and public relations strategies for organ donation because the public's awareness toward organ donation is closely related to their values, beliefs, and attitudes.

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Task Performance and Analysis of Organ Transplantation Coordinators in Korea (장기이식 코디네이터의 직무분석)

  • Kim, Hyung Sook;Yoo, Yang Sook;Cho, Ok Hee
    • Journal of Korean Clinical Nursing Research
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    • v.20 no.2
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    • pp.246-255
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    • 2014
  • Purpose: Along the process of organ transplantation, coordinators perform complex professional roles, playing as a key person among recipients, donors, family members and medical staffs. The purpose was to analyze the tasks of organ transplantation coordinators using DACUM method and to establish the basis for standardizing them in accordance with relevant laws and systems. Methods: Participants were consisted of 78 transplantation coordinators working at the medical centers in Korea. The questionnaire was administered to analyze the criticality, difficulty and frequency of task elements. Results: The job of organ transplantation was classified into five duties, 13 tasks, and 84 task elements. The five duties were recipient management, donor management, organ donation activation management, organ transplantation administration, and professional capability development. On the four-point scale: donor management was the duty with the highest criticality (3.68), organ donation activation management was the duty of highest difficulty (2.96), and recipient management was the duty of the highest frequency (3.32). Conclusion: This study will be useful for developing an educational program and as a reference of nurse practitioner qualifying examination. It is necessary to develop a comprehensive educational program for transplantation coordinators in order to support them to take their complex roles successfully.

Factors Influencing Will of Brain Death Organ Donation among University Students (대학생의 뇌사자 장기기증 의도에 영향을 미치는 요인+)

  • Son, Min-Seo;Kang, In-Soon;Jeon, Jung-Hee
    • The Korean Journal of Health Service Management
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    • v.12 no.4
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    • pp.191-202
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    • 2018
  • Objectives: The purpose of this study was to identify the factors influencing factors university students' will toward brain death organ donation. Methods: Data from 250 students were collected using a structured questionnaire. The data were analyzed using a chi-squared test, independent t-test, and binary logistic regression with the SPSS 23.0 program. Results: Factors influencing will of brain death organ donation were announcing to decision to engage in brain death donation(Confidence interval(CI)=3.02-32.14, p<.001), experience of having discussed brain death organ donation with others(CI=1.26-5.72, p=.011), intention to make advance directives(ADs)(CI=1.90-9.57., p<.001), and positive attitude toward ADs(CI=1.05-1.29, p=.004). Conclusions: The most important factors affecting the will of brain death organ donation were making an informed decision regarding organ donation, intending to make an AD, having engaged in organ donation conversation, and a positive attitude toward ADs.

The Associated Factors of the Intention and Registration for Organ Donation among Some Medical/Nursing Students in ICT based Society (ICT 시대 의학/간호학 전공학생의 장기기증 의향과 장기기증 등록 관련 요인)

  • Chung, Yoon-Young;Jeon, Yong-Hyun;Ryu, So-Yeon
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.1
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    • pp.67-76
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    • 2014
  • The purpose of this study was to examine the rate of intention and registration of organ donation and to find their associated factors among some medical and nursing students. The study subjects were 629 students who majored in the medicine and nursing in medical school. The data were collected from self-administerd questionnaire composed of intention and registration of organ donation and scales of knowledge and attitude toward organ donation. As a result, the rates of intention and registration of organ donation were 72.1% and 12.5%, respectively. The associated factors with the intention of organ donation were sex, judging the brain death as a death, attitude for organ donation. The associated factors with the registration of organ donation were major, knowledge scores and attitude scores of organ donation.

Accumulation and Organ distribution of protein Bound Cadmium in Rats compared with CdCl2 (단백질에 결합된 카드뮴과 CdCl2를 섭취한 쥐에서 카드뮴의 체내축적 및 분포의 차이)

  • 이명희
    • Journal of Nutrition and Health
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    • v.27 no.8
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    • pp.828-836
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    • 1994
  • A low level exposure experiment was conducted on growing rats to investigate the accumulation and organ distribution of protein bound cadmium compared with cadmium chloride. Male Sprague-Dawley rats were fed for 21days with one of the semisynthetic diets, which contains cadmium as either bovine liver- or kidney meal bound cadmium, cadmium chloride with uncontaminated liver meal or cadmium chloride without organ meal, in the levels of ca. 0.5, 1 and 1.5mg/kg diet, respectively. After 21days of exposure cadmium was accumulated in liver, kidney and gastrointestinal tracts depending upon cadmium levels in diet. Inspite of very low cadmium accumulation in whole blood, it tends also to increase with dietary cadmium levels. The blood cadmium concentration of animals fed organ meal containing diets was about 4-7 fold higher than that without organ meal, regardless of cadmium was intrinsically bound to protein or not. However, significant effects of organ protein on cadmium accumulation in liver, kidney and digestive tracts were not detectable, when cadmium was supplemented as cadmium chloride. On the other hands, animals fed diet containing ca. 1.5mg Cd/kg as organ bound cadmium retained more cadmium in liver, kidney and digestive tracts compared to cadmium chloride with organ meal, whereby the increase of cadmium concentration in kidney was greater then in liver. However, when the concentration of protein bound cadmium was<1mg/kg diet, organ bound cadmium was not significantly different from cadmium chloride in bioavailability and organ distribution. From this result it is suggested that the intestinal absorption of protein bound cadmium is influenced of the amount of cadmium bound in protein. When cadmium concentration in protein is relatively low, protein bound cadmium seems to be absorbed in the same way as cadmium ions are absorbed. However, when the concentration is high, at least a small amount of intact protein bound cadmium could be absorbed and accumulated selectively in kidney.

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A Study of Organ Weights in Nanjing(難經) (『난경(難經)』에 나타난 오장(五臟)의 무게에 대한 소고(小考))

  • Kim, Sang-Un;Jung, Hyun-Jong
    • Journal of Korean Medical classics
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    • v.28 no.3
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    • pp.67-77
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    • 2015
  • Objectives : This study is to verify the organ weights in Nanjing based on the weights of five viscera (五臟) in autopsy studies of modern times. Methods : Contents on organ weights from many annotations and articles on Nanjing were collected. Organ weights in autopsy studies dealt in many countries including China, India, U.S. and Korea were collected. Among the data, the average weights of liver, heart, spleen, pancreas, lung and kidney of males in the age of 18 to 60 were calculated, and the ratio of each organ was examined. Based on those results, the organ weights of Nanjing were evaluated. Results & Conclusions : There is a close correspondence between the organ weight ratios of liver(肝), heart(心), lung(肺) and kidney(腎) in Nanjing and those in autopsy studies. It proves that the organ weights in Nanjing were recorded based on an actual dissection. As a result of the analysis on autopsy studies, the average organ weights and the ratio among the organs were: liver 1416g(43.0%), heart 296g(9.0%), lung 1047g(31.8%), kidney 273g(8.3%), spleen 264g(4.5%) and pancrease 113g(3.4%). The weight of liver in Nanjing shall be 4 jin and 4 liang(4斤4兩) instead of 2 jin and 4 liang(2斤4兩) to occupy proper proportion out of other organs. It is highly possible that the weight of spleen(脾) in Nanjing is including the weight of pancrease(散膏), and the weight shall be 1 jin and 1 liang(1斤1兩) or 1 jin and 2 liang(1斤2兩) instead of 2 jin and 3 liang(2斤3兩) to occupy proper proportion out of other organs.