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Seed longevity of glyphosate resistant transgenic creeping bentgrass (Agrostis stolonifera L.) lines

  • Hancock, Daniel;Park, Kee Woong;Mallory-Smith, Carol A.
    • Journal of Ecology and Environment
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    • v.38 no.4
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    • pp.437-442
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    • 2015
  • Studies to estimate seed longevity and dormancy of creeping bentgrass (Agrostis stolonifera L.) were conducted from 2000 to 2005 at Corvallis and Hermiston, Oregon. Seeds from three transgenic glyphosate resistant creeping bentgrass lines, 48-10, 48-13, and ASR368, and one non-transgenic glyphosate susceptible line, SR1020, were used. Creeping bentgrass seeds were buried at 3, 18 and 31 cm in 2000 and removed 6, 12, 18, 24, and 51 months later. Soil type and climatic conditions were different at the two locations. At Corvallis, the soil was a Malabon silty clay loam, and the winters wet and mild. The soil at Hermiston was an Adkins fine sandy loam, and winters drier and colder. Seeds of all creeping bentgrass lines deteriorated faster at Corvallis than at Hermiston. The estimated half-lives of creeping bentgrass lines buried at Corvallis were 8.4 to 20.2 months, while those buried at Hermiston were 8.4 to 37.7 months. At both sites, seeds of the glyphosate resistant lines, 48-10 and 48-13, deteriorated faster than the susceptible line, SR1020. However, seed deterioration in the resistant line, ASR368, was slower than all other creeping bentgrass lines. Based on the germination test, exhumed intact seeds at Corvallis were more dormant than those at Hermiston. If buried, it could be expected that viable creeping bentgrass seeds will persist more than 4 years after the seeds are introduced to a site, but environmental conditions can influence both seed longevity and dormancy.

Development and Evaluation of an Oral Controlled Release Delivery System for Melatonin

  • Lee, Beom-Jin;Parrott, Keith A.;Sack, Robert L.;Ayres, James W.
    • Journal of Pharmaceutical Investigation
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    • v.23 no.3
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    • pp.9-18
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    • 1993
  • Sugar spheres loaded with melatonin (MT) were coated with $Aquacoat^{\circledR}$ to control the release rate of MT over 8 hours. A zero-order release pattern over 8 hours was obtained with 20% coating on 8-10 mesh beads in USP basket dissolution studies. MT in 20% coated beads was quite stable at room temperature with less than 5% MT degraded during 6 months' storage. Dissolution profiles were also unchanged after 6 months. An oral preparation containing MT-loaded uncoated beads for immediate release and 20% coated beads with $Aquacoat^{\circledR}$ for controlled release over 8 hours was evaluated in six human subjects. When total 0.5 mg MT as low dose (immediate release portion of MT, 0.1 mg) was administered to four subjects, average peak plasma MT concentration was reached at about 600 pg/ml and maintained at about 10 pg/ml over 8 hours. Plasma MT concentration-time profiles were similar in shape to computer-simulated profiles. However, maximal plasma MT concentrations were three times greater compared to computer simulated curve. These results suggest that MT dose, ratio of immediate and controlled release MT, and pharmacokinetic parameters selected are adjusted to mimic endogenous MT concentration-time curve. In another study, 0.2 mg MT having 10% of immediate release portion and 80% controlled release portion produced plasma MT concentration-time curve which is more similar to endogenous profiles. A low bioavailability (<20%) may result from extensive first pass metabolism and remaining amounts of MT from controlled beads. A good correlation between plasma MT concentration and urinary excretion rate of 6-sulphatoxymelatonin (6-STMT), a major metabolite of MT was observed. As plasma MT concentration increased, urinary excretion rate of 6-STMT increased concomitantly. The linear relation between plasma MT and urinary excretion rate of 6-STMT was statistically significant. This result suggests that urinary 6-STMT may be used as an index of circadian rhythms of MT in humans.

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Wewakamide A and Guineamide G, Cyclic Depsipeptides from the Marine Cyanobacteria Lyngbya semiplena and Lyngbya majuscula

  • Han, Bingnan;Gross, Harald;Mcphail, Kerry L.;Goeger, Doug;Maier, Claudia S.;Gerwick, William H.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.930-936
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    • 2011
  • Two new cyclic depsipeptides wewakamide A (1) and guineamide G (2) have been isolated from the marine cyanobacterium Lyngbya semiplena and Lyngbya majuscula, respectively, collected from Papua New Guinea. The amino and hydroxy acid partial structures of wewakamide A and guineamide G were elucidated through extensive spectroscopic techniques, including HR-FABMS, 1D $^1H$ and $^{13}C$ NMR, as well as 2D COSY, HSQC, HSQC-TOCSY, and HMBC spectra. The sequence of the residues of wewakamide A was determined through a combination of ESI-MS/MS, HMBC, and ROESY. Wewakamide A possesses a ${\beta}$-amino acid, 3-amino-2-methylbutanoic acid (Maba) residue, which has only been previously identified in two natural products, guineamide B (3) and dolastatin D (4). Although both new compounds (1,2) showed potent brine shrimp toxicity, only guineamide G displayed significant cytotoxicity to a mouse neuroblastoma cell line with $LC_{50}$ values of 2.7 ${\mu}M$.

Short-term cyclic performance of metal-plate-connected wood truss joints

  • Gupta, Rakesh;Miller, Thomas H.;Freilinger, Shawn M. Wicks
    • Structural Engineering and Mechanics
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    • v.17 no.5
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    • pp.627-639
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    • 2004
  • The objective of this research was to evaluate the performance of metal-plate-connected truss joints subjected to cyclic loading conditions that simulated seismic events in the lives of the joints. We also investigated the duration of load factor for these joints. We tested tension splice joints and heel joints from a standard 9.2-m Fink truss constructed from $38-{\times}89-mm$ Douglas-fir lumber: 10 tension splice joints for static condition and for each of 6 cyclic loading conditions (70 joints total) and 10 heel joints for static condition and for each of 3 cyclic loading conditions (40 joints total). We evaluated results by comparing the strengths of the control group (static) with those of the cyclic loading groups. None of the cyclic loading conditions showed any strength degradation; however, there was significant stiffness degradation for both types of joint. The results of this research show that the current duration of load factor of 1.6 for earthquake loading is adequate for these joints.

Selection of Promising Crimson Clover Cultivar in Paddy Field (논에서 적응 우수 Crimson Clover 품종 선발)

  • Kim, Won-Ho;Seo, Sung;Lim, Young-Cheol;Choi, Ki-Jun;Kim, Ki-Yong;Lee, Jong-Kyoung;Yoon, Bong-Ki
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.3
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    • pp.151-154
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    • 2007
  • This experiment was conducted to compare the agronomic characteristics and productivity of introduced crimson clover cultivars at paddy field of Naju(Jeollanam-Do Agricultural Research and Extension) from 2004 to 2006. The crimson clover cultivars used in this study were 4 varieties('Oregon com.' 'Tibbee', 'Contea', 'Linkarus') The fastest flowering variety was chinese milk vetch, while 'Linkarus' was the latest one as of as 5th May. The average dry matter(DM) content was 24.3% and crude protein(CP) content was 14.9%. 'Oregon com.' showed the highest CP content of 18.3% among crimson clover and Chinese milk vetch showed the highest CP content by 19.3%. Average acid detergent fiber(ADF), neutral detergent fiber(NDF) and total digestible nutrient(TDN) content were 35.1%, 45.3% and 58.9%, respectively. With by the winter hardness the productivity of crimson clover cultivars were severely various. Linkarus showed the highest DM yield with 6,986kg/ha but the DM yield of 'Tibbee' and 'Oregon com.' was low by 3,639 kg/ha and 1,980kg/ha, respectively. The results of this experiments indicated that the productivity of crimson clover cultivar varied from winter hardness, therefore we will consider the winter hardness as major factor when introducing crimson clover cultivars into Korea. According to the results obtained from this study, it is suggested that 'Linkarus' would be recommendable for fall sown crimson clover for dry matter production.

Gramene database: A resource for comparative plant genomics, pathways and phylogenomics analyses

  • Tello-Ruiz, Marcela K.;Stein, Joshua;Wei, Sharon;Preece, Justin;Naithani, Sushma;Olson, Andrew;Jiao, Yinping;Gupta, Parul;Kumari, Sunita;Chougule, Kapeel;Elser, Justin;Wang, Bo;Thomason, James;Zhang, Lifang;D'Eustachio, Peter;Petryszak, Robert;Kersey, Paul;Lee, PanYoung Koung;Jaiswal, kaj;Ware, Doreen
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.135-135
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    • 2017
  • The Gramene database (http://www.gramene.org) is a powerful online resource for agricultural researchers, plant breeders and educators that provides easy access to reference data, visualizations and analytical tools for conducting cross-species comparisons. Learn the benefits of using Gramene to enrich your lectures, accelerate your research goals, and respond to your organismal community needs. Gramene's genomes portal hosts browsers for 44 complete reference genomes, including crops and model organisms, each displaying functional annotations, gene-trees with orthologous and paralogous gene classification, and whole-genome alignments. SNP and structural diversity data, available for 11 species, are displayed in the context of gene annotation, protein domains and functional consequences on transcript structure (e.g., missense variant). Browsers from multiple species can be viewed simultaneously with links to community-driven organismal databases. Thus, while hosting the underlying data for comparative studies, the portal also provides unified access to diverse plant community resources, and the ability for communities to upload and display private data sets in multiple standard formats. Our BioMart data mining interface enable complex queries and bulk download of sequence, annotation, homology and variation data. Gramene's pathway portal, the Plant Reactome, hosts over 240 pathways curated in rice and inferred in 66 additional plant species by orthology projection. Users may compare pathways across species, query and visualize curated expression data from EMBL-EBI's Expression Atlas in the context of pathways, analyze genome-scale expression data, and conduct pathway enrichment analysis. Our integrated search database and modern user interface leverage these diverse annotations to facilitate finding genes through selecting auto-suggested filters with interactive views of the results.

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