• 한국동물위생학회지
• /
• 제44권4호
• /
• pp.217-225
• /
• 2021

We investigated the effect of temperature and stock density on the collection efficiency of oral fluid in the pig farm in Korea. Three pig farms with similar breeding environmental conditions were selected and four pens of each farm (total 12 pens) were tested for the collection efficiency of oral fluid from pigs. Collection rate was considered as significant when oral fluid was collected from 70% of pigs within a pen. In the case of growing pigs, when internal temperature of pig barn increased by one designated degree (5℃), the oral fluid collection rate significantly decreased by 24.7% (P<0.05). The collection rate of oral fluid also decreased by 7.1% (P<0.05) as the density rate increase by one designated degree (12.5%). It was estimated that the collection efficiency of oral fluid decreased when the internal temperature of pig barn was 30℃ or higher, or barn density is higher 25% or high. On the other hand, in the case of stall-housing sows, unlike growing pigs, there was no significant differences according to the temperature, so oral fluid collection was considered to be efficient even in hot season.

• 약학회지
• /
• 제55권2호
• /
• pp.145-153
• /
• 2011

A simultaneous detection and quantification method for determining the Phenylalkylamine derivatives, such as methamphetamine (MA), amphetamine (AM), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), ketamine (KT), norketamine (NKT), phentermine (PT), fenfluramine (FFA) and phenmetrazine (PM), in oral fluid was developed and validated according to international guidelines. The validated method was applied to actual oral fluid samples collected from drug abuse suspects. The recovery of phenylalkylamines from oral fluid collection devices was also assessed. Oral fluid specimens from 20 drug abuse suspects submitted by the police were collected using Salivette$^{TM}$, Quantisal$^{TM}$ or direct expectoration. The samples were screened using a biochip array analyzer. For confirmation, the samples were analyzed by GC-MS in selected-ion monitoring (SIM) mode after extraction using automated SPE with a mixed-mode cation exchange cartridge and derivatization with trifluoroacetic anhydride (TFAA). The results from the immunoassay were consistent with those from GC-MS. All the oral fluid samples gave positive results for MA, AM, PT and/or PM. The detection of phenylalkylamines in oral fluid can provide a better indication of recent use than urine or hair. Therefore, the oral fluid specimen was useful for demonstrating phenylalkylamines abuse in the driving under the influence of drug (DUID) as an alternative specimen for urine.

• 약학회지
• /
• 제54권4호
• /
• pp.226-231
• /
• 2010

Oral fluid has become increasingly popular as an alternative specimen in the field of driving under the influence of drugs (DUID) and work place drug testing. In this study, an analytical method for the detection and quantification of ${\Delta}^9$-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THC-COOH) in oral fluid by SPE and GC-MS was established and fully validated. The stability of THC and THC-COOH in oral fluid during storage was also determined by examining the THC and THC-COOH concentration changes depending on time and container materials. Oral fluid samples were kept over 21 days at room temperature, $-4^{\circ}C$ and $-20^{\circ}C$ in two different specimen collection tubes; glass and polypropylene tubes. Three replicates for each condition with different temperature and types of a container were analyzed at five different time points over 21 days. When oral fluid samples were stored in glass tubes, the loss of both THC and THC-COOH was less than 10% at all room temperature, $-4^{\circ}C$ and $-20^{\circ}C$. However, in polypropylene tubes, the loss of both THC and THC-COOH increased significantly over the study period. In particular, the concentration of THC decreased more rapidly than that of THC-COOH at room temperature and the maximal percentage of THC lost was 90.3% after 21 days. The result indicates that it would be necessary to collect oral fluid samples in glass containers and cool the samples until analysis in order to prevent the degradation of analytes.

• Journal of Oral Medicine and Pain
• /
• 제44권3호
• /
• pp.127-132
• /
• 2019

Septic arthritis of the temporomandibular joint (TMJ) is an uncommon disease caused by microbial pathogens through hematogenous infection, local spread, or iatrogenic infection. As the symptoms have an insidious onset, the early stage of septic arthritis is often confused with other diseases. A 49-year-old man was referred for increasing preauricular pain, swelling, and restricted mouth opening. He had been initially diagnosed as having a conventional temporomandibular joint disorder and trigeminal neuralgia and had been treated for the same. Imaging studies including panoramic view, lateral tomography, computed tomography with contrast enhancement, and magnetic resonance imaging were performed. Erosive bone change with displacement of the involved condyle, diffuse swelling of adjacent soft tissue, and fluid collection in the joint space were noted. Needle aspiration of the joint space and bacterial culture confirmed the diagnosis of septic arthritis of the TMJ and he was treated with antibiotic therapy and surgical drainage. Clinicians should always consider the diagnosis of septic arthritis of the TMJ in patients with preauricular pain or swelling.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제29권4호
• /
• pp.212-218
• /
• 2003

Matrix metalloproteinases (MMPs) play an important role in the normal morphogenesis, maintenance, and repair of matrix and also have important functions in pathologic conditions characterized by excessive degradation of extracellular matrix, such as rheumatoid arthritis, osteoarthritis, periodontitis and in tumor invasion and metastasis. In this study, expression of MMP-1 and -2 mRNA in retrodiscal tissue of the temporomandibular joint (TMJ) was examined and compared with magnetic resonance imaging (MRI) and surgical findings. MMP mRNAs in the retrodiscal tissue samples were detected by reverse transcription - polymerase chain reaction. TMJ internal derangement (ID) was categorized as normal disc position, disc displacement with reduction, early stage of disc displacement without reduction (DDsR) and late stage of DDsR. TMJ osteoarthrosis (OA) was classified with normal, mild and advanced OA. The amount of synovial fluid collection was divided into not detected, small, large and extremely large amount on MR T2-weighted imaging. Perforation and adhesion were examined during open surgery of the TMJ. Six out of 37 samples were excluded because of little amount of extracted total mRNA. MMP-2 mRNA was detected whole joints, and so the MMP-2 mRNA seems to be expressed normally in retrodiscal tissue. However, MMP-1 mRNA was expressed in 8 of 31 joints. Frequencies of MMP-1 mRNA expression according to the TMJ IDs, amount of synovial fluid and surgical findings made no significant difference. MMP-1 mRNA was detected more frequently in OA groups (7/16 joints, 43.8%) than in normal bony structure group (1/15 joints, 6.7%). Expression of MMP-1 mRNA in retrodiscal tissue might be related with OA of the TMJ.

• Toxicological Research
• /
• 제26권4호
• /
• pp.275-283
• /
• 2010

Placenta transfer study in non-human primate (NHP) is one of the crucial components in the assessment of developmental toxicity because of the similarity between NHP and humans. To establish the method to determine placenta transfer in non-human primate, toxicokinetics of valproic acid (VPA), a drug used to treat epilepsy in pregnant women, were determined in pregnant cynomolgus monkeys. After mating, pregnancy-proven females were daily administered with VPA at dose levels of 0, 20, 60 and 180 mg/kg by oral route during the organogenesis period from gestation day (GD) 20 to 50. Concentrations of VPA and its metabolite, 4-ene-VPA, in maternal plasma on GDs 20 and 50, and concentrations of VPA and 4-ene-VPA in placenta, amniotic fluid and fetus on GD 50 were analyzed using LC/MS/MS. Following single oral administration of VPA to pregnant monkeys, concentrations of VPA and 4-ene-VPA were generally quantifiable in the plasma from all treatment groups up to 4-24 hours post-dose, demonstrating that VPA was absorbed and the monkeys were systemically exposed to VPA and 4-ene-VPA. After repeated administration of VPA to the monkeys, VPA was detected in amniotic fluid, placenta and fetus from all treatment groups, demonstrating that VPA was transferred via placenta and the fetus was exposed to VPA, and the exposures were increased with increasing dose. Concentrations of 4-ene-VPA in amniotic fluid and fetus were below the limit of quantification, but small amount of 4-ene-VPA was detected in placenta. In conclusion, pregnant monkeys were exposed to VPA and 4-ene-VPA after oral administration of VPA at dose levels of 20, 60 and 180 mg/kg during the organogenesis period. VPA was transferred via placenta and the fetus was exposed to VPA with dose-dependent exposure. The metabolite, 4-ene VPA, was not detected in both amniotic fluid and fetus, but small amount of 4-ene-VPA was detected in placenta. These results demonstrated that proper procedures to investigate placenta transfer in NHP, such as mating and diagnosis of pregnancy via examining gestational sac with ultrasonography, collection of amniotic fluid, placenta and fetus after Caesarean section followed by adequate bioanalysis and toxicokinetic analysis, were established in this study using cynomolugus monkeys.

• Journal of Periodontal and Implant Science
• /
• 제41권1호
• /
• pp.3-9
• /
• 2011

Purpose: Infection with human immunodeficiency virus (HIV) remains a major global threat, and although the prevalence is comparatively still very low, the number of HIV-positive Koreans is increasing. However, there are no official guidelines as to how to treat people living with HIV/acquired immunodeficiency syndrome (AIDS) (PLWHA) or how to screen for potentially infectious people. This study assessed the level of knowledge and attitudes of dentists in Korea toward PLWHA, and their attitudes to screening patients for HIV infections. Methods: A cross-sectional prospective survey targeting dentists working in Korea was conducted using a self-administered questionnaire. Results: A satisfactory level of knowledge about HIV/AIDS and a relatively positive attitude toward PLWHA was found. Most of the respondents preferred rapid HIV testing using oral fluid as a specimen. The general attitude of dentists toward HIV/AIDS is sufficiently positive to enable provision of the best treatment to the patients in need. Conclusions: Most of the dentists require HIV testing in dental clinics. In spite of their needs, there are several obstacles. It is hoped that financial considerations and official legal requirements related to HIV testing strategies will be considered.

• 대한소아치과학회지
• /
• 제42권1호
• /
• pp.102-111
• /
• 2015

진단기법의 급속한 발전으로 인해 타액은 의학과 치의학 분야에서 가장 중요한 진단 샘플로서의 가능성이 있다. 타액은 혈장과 비교할 때 다음과 같은 장점을 갖는다. 단순하고 비 침습적이며 큰 집단의 검사 시 경제적으로 이용할 수 있다. 샘플 채취 시 감염의 위험이 극히 적고, 임상적으로 샘플 채취가 어려운 어린이나 장애인, 불안한 환자에서 유용하게 사용될 수 있다. 이 종설은 타액의 진단적 이용 가능성에 대하여 고찰하고 최근의 연구에 대한 요약을 제공하고자 한다.

• 치위생과학회지
• /
• 제14권4호
• /
• pp.525-536
• /
• 2014

2009년 9월부터 2010년 2월까지 대전 서구보건소, 서구 관저보건지소, 건양대학교 금연클리닉에 자발적으로 참여하고 연구에 동의한 금연대상자들을 기초시점, 2주차, 2개월, 4개월, 6개월, 12개월 시점에 추구조사하고, MMP-8, MMP-9, IL-$1{\beta}$의 농도를 ELISA법을 사용하여 측정하였다. 그 중 1년간 금연에 성공한 11명의 치은열구액내 변화사례를 관찰한 결과, 1. 각 대상자의 치은열구액내 MMP-8, MMP-9, IL-$1{\beta}$ 변화는 대상자에 따라 각기 다른 파동성을 보였다. 2. 금연기간 동안 치은열구액 내 MMP-8의 변화 경향은 5명의 대상자가 감소하였고 2명의 대상자가 증가하였으며, 4명의 대상자가 유지되었다. MMP-9의 변화 경향은 6명의 대상자가 감소, 2명의 대상자가 증가, 3명의 대상자가 유지되었다. IL-$1{\beta}$의 변화 경향은 5명의 대상자가 감소하였고 3명의 대상자가 증가하였고, 3명의 대상자가 유지되었다. MMP-8과 9이 증가되는 경향을 보이는 2명의 대상자는 다른 대상자에 비해 기초시점부터 각 바이오마커의 농도가 상당히 낮은 대상자였으며 증가폭은 다른 대상자에 비해 그 양이 적었다. 본 사례연구를 통해 금연은 치주에 관한 다른 처치 없이도 치은열구액 내 MMP-8, MMP-9, IL-$1{\beta}$ 농도를 감소시키거나 유지시킬 수 있는 가능성이 있다고 판단되었으나, 연구대상자수의 충분한 확보와 금연 성공률의 향상을 통해 통계분석이 가능한 추가적인 연구가 필요하다.

• Journal of Pharmaceutical Investigation
• /
• 제34권3호
• /
• pp.161-168
• /
• 2004

5-Fluorouracil (5-FU) is an antimetabolite anticancer agent active against many types of solid tumors. Tegafur (TF), a prodrug of 5-FU, is frequently used in combination with uracil as dihydropyrimidine dehydrogenase (DPD) inhibitory fluoropyrimidine. We studied the stability of 5-FU and TF in biological fluids of rats and determined their bioavailability (BA) and excretion into bile, and urine. The drug concentrations were analyzed by an HPLC method. At room temperature, there was a 14-30% decrease in the concentration of 5-FU and TF in bile, urine, and plasma specimen at 10 and $100\;{\mu}g/ml$ over 240 min. No significant difference was noted among the sample types or between two different concentrations of 10 and $100{\mu}g/ml$. The decrease in drug concentration was significantly less in samples kept on ice (6-12%) for both drugs. These data indicate that biological fluid samples containing 5-FU or TF in plasma, urine, or bile should be placed on ice during the sample collection. Following these storage guidelines, samples were collected after administration 50 mg/kg of each drug via i.v. or oral route. BA was 1.5 folds greater for TF (60%) than that of 5-FU (42%). Approximately 0.52 and 3.3% of the i.v. doses of 5-FU and TF was excreted into bile, respectively. Renal clearance of 5-FU was about 16% of its total body clearance. These results suggest that instability of 5-FU and TF in biological fluids should be considered in pharmacokinetic or pharmacogenomic studies.