• Journal of Pharmaceutical Investigation
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• 제37권3호
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• pp.159-166
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• 2007

In order to develop an optimum formulation for iontophoretic delivery of vitamine C-2-phosphate (VCP) from Carbopol hydrogel, we have investigated various factors which can affect the iontophoretic flux through skin. Such factors as drug concentration, current density, current profile, current duration, ionic strength, pH and percutaneous enhancers (ethanol, glycerine, propylene glycerol, sorbitol, urea) were studied. Compared to the flux by passive or anodal delivery, the flux by cathodal delivery increased markedly, and thus, only cathodal delivery was used in the rest of the flux experiments. Flux increased linearly as the drug concentration and current density increased. As the duration of current application increased from 30 min to 120 min, flux increased linearly, however the AUC was not directly proportional to the applied amount of current. Flux also increased as the pH increased, possibly due to the increase in ionization of phosphate group. As the ionic strength increased, flux decreased. No significant increase in flux was observed after enhancer application, indicating that the effect of current on flux is dominating over the effect of percutaneous enhancers (P>0.05). These results provide important informations that are needed for optimal formulation of iontophortic delivery for VCP.

• 한국축산식품학회지
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• 제37권3호
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• pp.449-455
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• 2017

Chocolate milk is one of the most commonly used non-fermentative dairy products, which, due to high level of sucrose, could lead to diabetes and tooth decay among children. Therefore, it is important to replace sucrose with other types of sweeteners, especially, natural ones. In this research, response surface methodology (RSM) was used to optimize the ingredients formulation of prebiotic chocolate milk, date syrup as sweetener (4-10%w/w), inulin as prebiotic texturizer (0-0.5%w/w) and carrageenan as thickening agent (0-0.04%w/w) in the formulation of chocolate milk. The fitted models to predict the variables of selected responses such as pH, viscosity, total solid, sedimentation and overall acceptability of chocolate milk showed a high coefficient of determination. The independent effect of carrageenan was the most effective parameter which led to pH and sedimentation decrease but increased viscosity. Moreover, in most treatments, date syrup and inulin variables had significant effects which had a mutual impact. Optimization of the variables, based on the responses surface 3D plots showed that the sample containing 0.48% (w/w) of inulin, 0.04% (w/w) of carrageenan, and 10% of date syrup was selected as the optimum condition.

• 한국자원식물학회지
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• 제30권6호
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• pp.686-692
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• 2017

The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water ($90^{\circ}C$) treatment as a control. In our findings, highest TP (118 mg/g), TF (38 mg/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

• Journal of Pharmaceutical Investigation
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• 제28권2호
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• pp.93-98
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• 1998

We have developed liposomes which can be easily prepared with inexpensive lipid, have enhanced stability, and can efficiently deliver DNA into the COS-l cells, Liposome formulations were prepared using cationic materials such as dimethyldioctadecyl ammonium bromide (DDAB), cetyltrimethyl ammonium bromide(CTAB), We investigated the effect of cationic liposome formulations on in vitro DNA transfection, DDAB-containing liposomes showed increased transfection efficiency which was 3.2-fold as much as that by $Lipofectin^{\circledR}$, but CTAB-containing liposomes were inactive in gene transfection. The effect of colipid of DDAB-containing liposome was also investegated. As a colipid, dioleylphosphatidylethanolamine(DOPE) and cholesterol did altered the transfection efficiency of DDAB-containing liposomes. And increased DDAB concentration lowered the transfection efficiency. The optimum amount of liposomal formulation was $10\;{\mu}M$ for $1\;{\mu}g$ of DNA. In the experiment of stability, DOPE-containing liposomes formulation showed a broad size distribution and separation of two major peaks on a 5th day of preparation, but liposomes containing cholesterol was stable for 10 days. DDAB-containing liposomal DNA delivery system was prepared easily and was stable.

• 한국자동차공학회논문집
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• 제18권4호
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• pp.1-7
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• 2010

Large quantity of bending deformation as well as irregular rotating torque fluctuation are the main struggles of the balance shaft module during a high speed rotation. Since two issues are much sensitive to the location of both supporting bearing and unbalance mass at a balance shaft, it is recommended to construct a design strategy on balance shaft at the early stage so as to save developing time and effort before approaches to the detailed design process. In this paper, an optimal design formulation is proposed to minimize the elastic strain energy due to bending as well as the kinematic energy of polar moment of inertia in rotation. Case studies of optimal design are conducted for different mass ratio as well as linear combination of objective function and its consequence reveals that global optimum of balance shaft model is existed over possible design conditions. Simulation shows that best locations of both supporting bearing and unbalance are globally 20% and 80%, respectively, over total length of a balance shaft.

• 한국식생활문화학회지
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• 제27권2호
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• pp.202-210
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• 2012

The purpose of this study was to bake sweet rice muffins with oak mushroom ($Lentinus$ $edodes$) powder. The process included substituting sweet rice flour for cake flour and adding oak mushroom powder. This study determined the optimal mixing conditions of oak mushroom muffins by adjusting the amounts of oak mushroom powder, whole eggs, and soybean oil. The mixing conditions for the oak mushroom muffins included 3 categories: oak mushroom powder (X1), whole eggs (X2), and soybean oil (X3) by Central Composite Design (CCD) which was optimized by Response Surface Methodology (RSM). Oak mushroom muffin formulation was optimized using rheology. Yellowness (p<0.001) and redness (p<0.05) displayed a linear model pattern, whereas lightness (p<0.05) was represented by a quadratic model. Among the sensory properties, variables that appeared to show significant values such as appearance (p<0.5), texture (p<0.5), and overall quality (p<0.5) were used to identify optimums. The result of mechanical properties showed significant values in gumminess (p<0.5) and chewiness (p<0.5). The numerical and graphical methods used in this study determined that the optimum formulation for oak mushroom powder sweet rice muffins was 8.75 g of oak mushroom powder, 235.95 g of whole eggs, and 19.93 g of soybean oil.

• Structural Engineering and Mechanics
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• 제36권5호
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• pp.561-573
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• 2010

The potential of the liquid column vibration absorber (LCVA) as a seismic vibration control device for structures has been explored in this paper. In this work, the structure has been modeled as a linear, viscously damped single-degree-of-freedom (SDOF) system. The governing differential equations of motion for the damper liquid and for the coupled structure-LCVA system have been derived from dynamic equilibrium. The nonlinear orifice damping in the LCVA has been linearized by a stochastic equivalent linearization technique. A transfer function formulation for the structure-LCVA system has been presented. The design parameters of the LCVA have been identified and by applying the transfer function formulation the optimum combination of these parameters has been determined to obtain the most efficient control performance of the LCVA in terms of the reduction in the root-mean-square (r.m.s.) displacement response of the structure. The study has been carried out for an example structure subjected to base input characterized by a white noise power spectral density function (PSDF). The sensitivity of the performance of the LCVA to the coefficient of head loss and to the tuning ratio have also been examined and compared with that of the liquid column damper (LCD). Finally, a simulation study has been carried out with a recorded accelerogram, to demonstrate the effectiveness of the LCVA.

• Archives of Pharmacal Research
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• 제24권4호
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• pp.355-359
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• 2001

A novel HPLC method with electrochemical detection has been developed for the determination of recombinant human epidermal growth factor (rhEGF) in pharmaceutical products. rhEGF was separated from other components in formulation on a reversed-phase C18 column with 24% acetonitrile in 0.1 M phosphate buffer (pH 4.75). The optimum electrochemical oxidation of EGF was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to electroactive tyrosine, tryptophan, methionine, and arginine residues. The quantitation range was from 1.0 to 200 ng of rhEGF with the linear correlation coefficient greater than 0.999. The method was successfully applied for the quantitation of rhEGF in a pharmaceutical preparation.

• 농약과학회지
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• 제10권1호
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• pp.28-35
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• 2006

Flucetosulfuron 수면부상성 입제의 제형요소 및 효능을 최적화하기위한 일련의 실험을 실시하였다. SPA와 xanthan gum을 혼합하여 입제로 제제한 경우 조립성과 부상성이 우수하여 점착제로 선발하였으며 증량제는 조립성, 부상성 및 붕괴성이 우수한 KCl을 선발하였다. 또한 습윤 및 분산제로는 분산성이 가장 우수한 Surfynol 440을 선발하였다. 입제의 입경은 부상성과 분산성을 고려 1.0 mm로 결정하였다. 본 연구로 선발된 부제를 이용해 조제한 flucetosulfuron 수면부상성 입제를 실내 간이 포장 및 야외 포장에서 제초활성을 평가한 결과 잡초를 처리지점에서 15 m에 다다르는 지점까지 우수하게 방제하였다. 따라서 본 수면부상성 입제 기술은 flucetosulfuron을 비롯한 노동력 절감형 제초제 개발에 사용될 수 있을 것으로 기대된다.

• 동아시아식생활학회지
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• 제12권5호
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• pp.364-369
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• 2002

본 연구는 천연 자원인 뽕나무에서 혈당강하 효능을 가진 뽕잎(상엽)의 열수 추출법을 이용하여 상엽 추출물의 공정화 확립과 Formulation 개발을 위해 상엽 추출물과 silk peptide 및 한약재를 첨가한 시제품을 동물실험을 통하여 Formulation을 최종 결정하여 제품화하여 기능성 음료를 개발하고자 하였다. 연구 결과, 상엽 추출의 최적 조건은 상엽 크기는 3mm 이하로 하고, 2/3 정도 packing된 면포자루로 포장된 상엽을 사용하며, 상엽 사용량은 50~100 g/L, 추출 온도는 9$0^{\circ}C$, 추출 시간은 2 hr으로 하였으며 이때 수율은 20~25%였다. 또한 시료 배합에 따른 혈당강하 실험을 통하여 상엽 추출물과 silk peptide 의 농도는 각각 1%, 0.1%일 때 혈당강하 작용이 가장 우수하였으며 부재료로 사용된 둥글레는 상엽추출물의 10% 첨가한 경우가 혈당강하작용이 가장 컸으며, polydextrose도 효과가 있으므로 첨가하는 것이 바람직하다고 사료된다.