• Title/Summary/Keyword: Optimum Temperature

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Strategy to Recover Rare Earth Elements from a Low Grade Resource via a Chemical Decomposition Method (화학적 분해법을 이용한 난용성 자원으로부터 희토류 회수 특성 연구)

  • Kim, Rina;Cho, Heechan;Jeong, Jinan;Kim, Jihye;Lee, Sugyeong
    • Resources Recycling
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    • v.29 no.1
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    • pp.17-24
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    • 2020
  • In this study, rare earth elements (REE) leaching from a refractory REE ore containing goethite as a major gangue mineral was conducted, introducing a two-stage method of chemical decomposition-acid leaching. At the chemical decomposition step, using one of alkaline agent, NaOH, the ore was decomposed, changing NaOH concentration from 20 to 50 wt% at 10% (w/w) of pulp density and the maximum temperature achieved without boiling at each NaOH concentration. With increasing NaOH concentration, light REE (Ce, La and Nd) and iron were concentrated in the solid phase which is the decomposed product, while aluminum (Al) and phosphorus (P) were removed to the liquid phase, and their concentrations in the solid phase were down to 0.96 and 0.17%, respectively. In addition, through XRD analysis, it was found that the crystallinity of goethite was considerably decreased. At the acid leaching step, the product decomposed by 50 wt% NaOH was leached at 3.0 M HCl and 80 ℃ for 3 hr, then the REE leaching efficiency was above 94% (Ce 80%), and the leaching efficiencies of Al and P were decreased to 12 and 0%, respectively. Therefore, in terms of both REE leaching efficiency and impurity removal, those decomposition and leaching conditions were chosen as optimum processing methods of the investigated material. In terms of REE leaching mechanism, because REE and iron leaching efficiencies showed the positive correlation each other, so it can be concluded that decreasing crystallinity of goethite affect the improvement of REE leaching.

Enzymatic Characteristics of Polyphenol Oxidase from Apple (Ralls Janet) (사과(Ralls Janet) Polyphenol Oxidase의 효소학적(酵素學的) 성질(性質))

  • Chung, Ki Taek;Seu, Seung Kyo;Song, Hyung Ik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.316-322
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    • 1983
  • In order to obtain elementary data of enzymatic browning of apples and apple products and to examine effectively inhibitory method of browning, we extracted polyphenol oxidase (EC 1.10.3.1) from apple (Ralls Janet) and investigated its general properties. The optimum conditions for the enzyme reaction were pH 6.0 and temperature of $30^{\circ}C$. The enzyme was very stable at pH 4.0, and at the range of pH 5.0-9.0 its activity was above 80% compared with pH 4.0. The enzyme was very stable by heating at $40^{\circ}C$ for 1 hour, and almost 50% of enzyme activity was lost by heating at $60^{\circ}C$ for 30 minutes. The polyphenol oxidase activity was enhanced by the addition of $Cu^{2+}$ and $Mn^{2+}$, respectively, meanwhile $Na^+$, $Hg^{2+}$ and $Co^{2+}$ inhibited the enzyme activity. The enzyme activity was greatly decreased in the presence of inhibitors such as cysteine, sodium metabisulfite and ascorbic acid. The polyphenol oxidase greatly catalyzed the oxidation of o-diphenols such as chlorogenic acid and catechol, which suggests that main substrate of polyphenol oxidase is o-diphenol compounds.

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Quality Characteristics of Low-Dose Electron Beam Irradiated-Imported Navel Orange during Storage at Low Temperature (3°C) (저선량 전자선 조사 수입 오렌지의 저온 저장 중 품질 특성)

  • Cho, Yun-Jeong;Kim, Kyung-Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.1
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    • pp.128-136
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    • 2015
  • This study investigated the effects of low-dose electron beam irradiation treatment on physicochemical and sensorial properties of imported navel oranges during storage at $3^{\circ}C$ for 45 days. The samples were irradiated at doses of 0.2, 0.4, 0.6, 0.8, and 1.0 kGy, and changes in their color values, hardness, Brix/acid ratio, total sugar contents, reducing sugar contents, vitamin C contents, and sensory evaluation were investigated. There were no significant differences between non-irradiated and irradiated samples in terms of color values, Brix/acid ratio, total sugar contents, total reducing sugar contents, and vitamin C contents. Hardness of irradiated sample at 1 kGy decreased significantly in the early storage period, but the difference between non-irradiated and irradiated samples decreased again at the end of storage. For the sensory evaluation, scores of color, sweetness, flavor, and overall acceptability decreased as irradiation dose and storage period increased. Samples irradiated at over 0.8 kGy showed low preference in all scores except color. These results suggest that electron beam irradiation below 0.6 kGy does not affect physicochemical and sensory properties; thus, electron beam irradiation up to 0.6 kGy in imported navel oranges is optimum for minimizing quality changes and disinfestation treatment simultaneously.

Optimal Culture Conditions and Food Waste Decomposition Effects of Mixed Strains Separated from Traditional Fermented Food and Soils (전통발효식품과 토양으로부터 분리된 혼합균주의 최적생육조건 및 음식물쓰레기 분해 효과)

  • Kim, Min-Sun;Kim, Hee-Jeong;Jung, Eun-Seon;Park, Ju-Yong;Chae, Jong-Chan;Hwang, Kwontack;Lee, Seung-Je
    • Journal of Chitin and Chitosan
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    • v.23 no.4
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    • pp.285-292
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    • 2018
  • In this study, for the purpose of decomposing food waste, the strain was screened from traditional fermented food and soils. The enzyme activity (protease, amylase, cellulase, lipase) experiment was carried out using the paper disc method in 212 strains isolated from 5% NaCl media. Among them, only the strains having enzyme activity of more than 2 (soil) or more than 4 (traditional fermented food) with the halozone of enzyme activity of 15 mm or more were selected first, and microorganism identification through 16S rRNA sequencing was performed. Finally, were identified such as Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus siamensis, Bacillus licheniformis, Bacillus aquimaris, Bacillus megaterium, Bacillus koreensis, Bacillus stratoshericus, Bacillus aryabhattai, Bacillus safensis, Marinobacter hydrocarbonoclasticus. 11 species of mixed strains were confirmed that the culture time was 24 hours, the incubation temperature was $30^{\circ}C$ and the optimum pH was 7.0. In order to confirm the degree of decomposition of standard food wastes (100 g) by treating 11 kinds of mixed strains (25%), solid content of more than $2000{\mu}m$ was determined to be 103 g for the sterilized water group and 18 g for the mixed strains group. And the rest was decomposed to a size of less than $2000{\mu}m$.

Culture Conditions of Garlic Resistant Lactic Acid Bacteria for Feed Additives (사료첨가용 생균제 개발을 위한 마늘 내성 유산균의 배양 조건)

  • Kim, Yu-Jin;Jang, Seo-Jung;Park, Jung-Min;Kim, Chang-Uk;Park, Young-Seo
    • Food Engineering Progress
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    • v.14 no.1
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    • pp.65-74
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    • 2010
  • Culture conditions of L. plantarum TJ-LP-002, the garlic resistant strain isolated from pakimchi (green onion kimchi), were investigated for the use of feed additives. Acetic acid, citric acid, lactic acid, and tartaric acid were detected in the culture supernatant, and especially the concentrations of lactic acid and acetic acid significantly increased during cultivation. The antimicrobial activity of L. plantarum TJ-LP-002 was not affected by proteases, calatase or cellulase, which showed that the antimicrobial activity might be due to the production of acids rather than proteinaceous antimicrobial substances. L. plantarum TJ-LP-002 was resistant to neomycin sulfate, spectinomycin dihydrochloride, and lincomycin hydrochloride, sensitive to streptomycin sulfate, and intermediate resistant to ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, and kanamycin sulfate. The optimum initial pH of medium, fermentation temperature and time for the cell growth and antibacterial activity were pH 7.0, 30${^{\circ}C}$ and 24hr, respectively. The optimal composition of culture medium for the cell growth and antimicrobial activity was 3%(w/v) glucose as a carbon source, 3%(w/v) yeast extract as a nitrogen source, and manganese sulfate and ammonium citrate as inorganic salts. The combinatorial supplementation of these inorganic salts, rather than sole addition as an inorganic salt, resulted in better antibacterial activity.

A Mid-Maturing Apple Cultivar "Hongso", High Density Cultivation Type having a Good Taste (밀식재배형 식미우수 중생종 사과 "홍소(紅笑)")

  • Kim, Mok Jong;Kwon, Soon Il;Paek, Pong Nyeol;Nam, Jong Chul;Kang, Sang Jo;Shin, Yong Uk;Hwang, Jung Hwan;Kang, In Kyu;Choi, Cheol
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.556-559
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    • 2009
  • "Hongso" was originated from a cross between "Yoko" and "Hongro" in NIHHS, RDA, in 1989. "Hongso" was preliminarily selected in 2002 for its high fruit quality. After regional adaptability test at five sites from 2003 to 2006 as "Wonkyo Ga-30" it was finally selected in 2006. Optimum harvest time is early September which is similar to that of "Hongro". Fruit shape is globose conical and skin color is light red. Mean fruit weight is 295g and soluble solids content is 14.1oBrix. Fruits acidity is 0.34%, which is higher than that of "Hongro"(0.23%). It has a good taste for harmony of sugar and acidity. Storability is 3 weeks in room temperature. It is apt to russet on fruit skin. It is susceptible to Bitter rot. "Hongso" has a good cross compatibility with major cultivars such as "Fuji", "Hongro" and "Tsugaru". Tree habit is semi-spreading and tree vigor is weak. "Hongso" is high-density cultivation type cultivar, because it has a precocity, spur-type.

Characteristics of Seed Germination and Seedling Growth of Native Hydrangea serrata for. acuminata (자생 산수국의 종자 발아와 유묘 생육 특성)

  • Lee, Seung Youn;Kim, Kwang Jin;Lee, Jeong Sik
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.134-142
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    • 2008
  • This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

Wheat-Rice Double Cropping System in Rice Fields of the Cheonan Area for the Production of Domestic Wheat (국산밀 생산을 위한 천안지역 논에서의 밀-벼 이모작 작부체계)

  • Kim, Young-Bok;Yang, jing;Yoon, Seong-Tak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.3
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    • pp.234-245
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    • 2019
  • In order to select the best varieties and cultivation methods for the production of domestic wheat for Hodugwaja(a walnut shaped confection), we carried out a 3-year experiment (2015~2017) to investigate the effect of different factors (crop variety, planting date, nitrogen fertilization) in a double cropping system (wheat then rice) on crop yields in the Cheonan area. Rice is the second crop in the system, and requires an accumulated temperature for 40 days of about $840{\sim}930^{\circ}C$ to ensure ripening. Transplanting dates for rice between June 29 and July 6 were suitable; transplanting on or after July 13 does not ensure ripening. The daily hours of sunshine ranged from 6.3 to 6.5 hours, which were slightly higher than the optimum of 6.0 to 6.1 hours. The higher the nitrogen fertilizer treatment, the higher culm length, and spike length of wheat. The yield of wheat per 10a tended to increase as the amount of nitrogen fertilizer increased. Wheat yields were highest for the Sooan variety, followed by Goso, followed by Keumgang. The number of days from transplanting to heading of rice were shortest for the Jopyeong variety followed by Unkwang, followed by Haedam. The yield of milled rice per 10a increased as the transplanting date was earlier and the transplanting date of June 9 showed the average yield of more than 500 kg in three varieties. From the results of the experiment, we recommend the Goso wheat variety and the Haedam rice variety for growing in a double cropping system under the climatic conditions of the Cheonan area.

Identification and Chemical Control of Gray Snow Molds Caused by Typhula spp. on Golf Course in Korea (우리나라의 골프코스에서 Typhula spp.에 의해 발생하는 설부병의 동정 및 방제)

  • Kim, Jeong-Ho;Shim, Gyu-Yul;Lee, Hye-Min;Moon, Hyo-Sun;Kim, Young-Ho
    • Asian Journal of Turfgrass Science
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    • v.21 no.2
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    • pp.147-154
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    • 2007
  • In March of 2004, gray snow mold (Typhula blight) caused by Typhula spp. occurred on perennial ryegrass (Lolium perenne L.) and Kentucky bluegrass (Poo pratensis L.) at MuJu golf courses in Jeonbuk Province. Leaves in the affected areas were matted together and frequently covered with white to grayish mycelia. Sclerotia were formed on the leaf blade, leaf sheath, or crown regions. The fungus isolated from the diseased leaf formed whitish mycelium, clamp connections, and light pink to brown, irregular-shaped small sclerotia of less than 1.4 mm in diameter, which are characteristic to Typhula incarnata. Optimum temperature ranges for mycelial growth were $5^{\circ}C$ to $15^{\circ}C$. The causal organism was confirmed to be T. incarnata as the partial sequence of its ribosomal RNA ITS1 (internal transcribed spacer) region was 91% homologous to those of T. incarnata in GenBank database. Out of the 14 fungicides tested fur antifungal activity in vitro, 10 fungicides including iprodione, tebuconazole, polyoxin D, flutolanil, hexaconazole, tolclofos-methyl, fosetyl-Al, mepronil, pencycuron+tebuconazole, and fenarimol completely inhibited fungal growth at their recommended concentrations. In the field test, these fungicides and others such as thifluzamide and thiram effectively controlled the gray snow mold of turfgrass with some variable degrees of control efficacies.

Isolation and Cultural Characterization of Antibacterial Substance Producing Microbes (항균성 물질 생산 균주의 분리 및 배양학적 특성)

  • Park, Seok-Kyu;Cho, Young-Su;Shon, Mi-Yae;Gal, Sang-Wan;Lee, Sang-Won
    • Food Science and Preservation
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    • v.14 no.2
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    • pp.194-200
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    • 2007
  • In order to enhance the functionality and storage period of traditional fermented foods, the strain CH-14, which To enhance the quality of traditional fermented foods, and to lengthen acceptable storage periods, a bacterial strain, CH-14, showing potent enzyme activities and antibacterial capabilities, was isolated and characterize4 The bacterium wn Gram-positive, catalase-positive, oxidase-negative, formed endospores, expressed flagella, was rod-shaped, and had dimensions of 0.5 0.7m and 3.5 4.2m. The bacterium CH-14 was identified as Bacillus subtilis using Bergey's Manual of Systematic Bacteriology, Bergey's Manual of Determinative Bacteriology, and an API 50 CHL Carbohydrate Test Kit. An optimum growth medium contained 2% (w/v) cellobiose as a carbon source, a mixture of 0.5% (w/v) yeast extract and 0.5% (w/v) peptone as nitrogen sources, and 0.05% (w/v) $MgSO_4{\cdot}7H_2O$. The optimal culture temperature and the optimal initial pH were in the ranges of 30 $45^{\circ}C$ and 4.5 10.0, respectively. Maximum production of the antibacterial substance occurred after 24h of culture. The minimum inhibitory concentrations of the antibacterial substance were 5mg bacterial dry weight/mL against E. coli and P. mirabilis, and 10 mg/mL against S. aureus, S. enteritidis and V. parahaemolyticus.