• Journal of the Korean Wood Science and Technology
• /
• 제48권6호
• /
• pp.861-868
• /
• 2020

The use of polyphenol extracts from radiata pine (Pinus radiata) bark as raw materials for bio-foams was investigated along with the optimal NaOH extraction conditions. The targeted yield of alkaline extracts was 60%, and the targeted pH was 11 to 12. The radiata pine bark was composed of 70% of a 1% NaOH extract, which contained mainly polyphenols, such as proanthocyanidin (PA). As the particle size of the bark decreased, the yield of the 1% NaOH extracts increased from 57 to 87%. A range of NaOH concentrations, liquor ratios, and extraction times were explored to establish an economic polyphenol extraction method. More than 60% of the alkaline extract was extracted, and the pH of the extract was approximately 12 when the optimum extraction conditions were employed, i.e., a liquor to bark ratio of 5:1, a NaOH dosage of 17 to 18% based on the bark weight, and a 1 h extraction time. Following neutralization of the alkaline extract, structural analysis indicated severe structural changes in the PA during the alkaline extraction. Because the alkaline extract was barely soluble in the solvent used for the structural analyses, it is assumed that chemical modification is required to increase the solubility of the alkaline extract for the production of bio-foams.

• 한국강구조학회 논문집
• /
• 제12권6호
• /
• pp.629-638
• /
• 2000

알고리즘의 용이성과 전역적 최적해로의 수렴가능성 등의 이점을 가진 SA알고리즘은 구조최적화문제에 활발하게 적용되고 있으나 냉각스케줄의 설정, 모호한 종료기준, 과도한 반복해석 등의 문제점을 가지고 있다. 그러므로 본 논문에서는 기존 SA알고리즘의 단점을 보완한 MSA 알고리즘을 개발하고자한다. MSA 알고리즘은 수렴에 요구되는 반복수를 감소시키고 국부최소점이 많은 동적최적화문제의 초기설계 선택의 자율성을 확보하기 위하여 SQ 및 SA의 2단계로 구성하여 개발하였다. 또한 기존 연구에서 제안된 냉각 스케줄에 의한 수렴성 등을 비교분석하여 구조최적화에 적합한 냉각스케줄을 제안하여 그 성능을 평면가새골조 구조물의 최적내진설계에 적용하여 분석하였다.

• 한국자동차공학회논문집
• /
• 제5권3호
• /
• pp.182-190
• /
• 1997

Recently to develope an automobile with better properties, much researches and investments are executed in many countries. In this paper, it is intended to minimize the weight of an engine block without changing the dynamic characteristics. The weight minimization is executed by the sensitivity of the natural frequency of the engine block. To decrease the engine weight, much less thickness than the original thickness of the engine is selected to initial value and the structure modification is performed to recover the dynamic characteristics of the engine. Here, the original thickness of the engine is 8mm and the initial thickness is selected to 5mm, 6mm and the number if the natural frequencies fitted are 2, 6, 7, respectively. As the results, it is found that; (1) the weight of each case could be reduced without changing the objective natural frequencies. Specially, in the case of fitting 2 natural frequencies with 5mm initial thickness the weight could be reduced to 4.21kg(23.3% for engine weight). (2) according to the driving frequency range of engine, the weight minimization could be performed choicely, (3) improving a vibration characteristics of a orignal structure, the weight minimization could be performed.

• Structural Engineering and Mechanics
• /
• 제52권1호
• /
• pp.205-220
• /
• 2014

This paper is concerned with the modification of multidisciplinary feasible formulation for MDO problems using the integrated coupled approximate models. A drawback of conventional MDFs is the numerical difficulty in decomposing the design variables and deriving the coupled equations of state. To overcome such a drawback of conventional methods, the coupling in analysis and design is resolved by approximating the state variables in each discipline by the response surface method and by modifying the optimization formulation using the corresponding integrated coupled approximate models. The validity, reliability and effectiveness of the proposed method are illustrated and verified through two optimization problems, a mathematical MDF problem and the multidisciplinary optimum design of suspension unit of wheeled armored vehicle.

• 청정기술
• /
• 제25권1호
• /
• pp.33-39
• /
• 2019

활성탄(activated carbon, A.C)은 휘발성 유기화합물(volatile organic compounds, VOCs) 제거를 위해 가장 많이 사용되고 있지만 흡/탈착 시 열화현상으로 인한 화재위험성, 잦은 교체 주기로 인한 비용 부담, 수분에 의한 성능 저하 등의 문제점을 가지고 있다. 이러한 문제들을 해결하기 위하여 소수성 제올라이트 흡착제가 연구되고 있다. 본 연구에서는 소수성 개질법 중 하나인 수증기처리 및 산 처리를 통해 $NH_4{^+}Y$-zeolite를 소수성 Y-zeolite로 합성하여 높은 표면적, 열적 안정성과 습도저항성을 확보하고자 하였다. Y-zeolite와 개질된 Y-550-HN, Y-600-HN, Y-650-HN의 흡착성능은 23, 38, 77, $61mg\;g^{-1}$으로 나타났으며, 소수성 개질 정도를 확인할 수 있는 지표인 Si/Al ratio 변화를 XRF 분석으로 확인하였다. 그 결과, Y-zeolite를 개질하였을 때 흡착성능이 증진되었고, Si/Al 비는 Y, Y-550-HN, Y-600-HN, Y-650-HN 순으로 각각 3.1765, 6.6706, 7.3079, 7.4635 임을 확인하였다. 반면에 높은 열처리 온도에 의한 구조적 결정화가 성능 저하에 영향을 미칠 수 있음을 확인하였다. 반면에 Y-zeolite의 최적 열처리 온도가 존재하며, 이와 같은 최적 개질 조건 연구는 높은 내구성과 안정성을 갖는 흡착제를 제조할 수 있는 조건으로써 향후 활성탄을 대체할 수 있을 것으로 판단하였다.

• Earthquakes and Structures
• /
• 제19권3호
• /
• pp.197-214
• /
• 2020

Diagrid structures have been introduced as a fairly modern lateral load-resisting system in the design of high-rise buildings. In this paper, a novel diagrid system called tube-in-tube diagrid building is introduced and assessed through pushover and incremental dynamic analyses. The main objectives of this paper are to find the optimum angle of interior and exterior diagrid tube and evaluate the efficiency of diagrid core on the probability of collapse comparing to the conventional diagrid system. Finally, the seismic performance factors of the proposed system are validated according to the FEMA P695 methodology. To achieve these, 36-story diagrid buildings with various external and internal diagonal angles are designed and then 3-D nonlinear models of these structures developed in PERFORM-3D. The results show that weight of steel material highly depends on diagonal angle of exterior tube. Adding diagrid core generally increases the over-strength factor and collapse margin ratio of tall diagrid buildings confirming high seismic safety margin for tube-in-tube diagrid buildings under severe excitations. Collapse probabilities of both structural systems under MCE records are less than 10%. Finally, response modification factor of 3.0 and over-strength factor of 2.0 and 2.5 are proposed for design of typical diagrid and tube-in-tube diagrid buildings, respectively.

• 한국구조물진단유지관리공학회 논문집
• /
• 제20권1호
• /
• pp.95-103
• /
• 2016

대다수의 흙막이 공사 계측현장에서 적용하고 있는 절대치 관리방법은 관리기준치와 계측결과를 절대 비교함으로서 공사현장의 안전성을 평가하는 기법이다. 따라서, 안전성 평가의 판단기준이 되는 관리기준치는 평가결과에 직접적으로 영향을 미치게 된다. 즉 아무리 최적의 계측시스템이 적용된 현장이라고 하더라도 관리기준치가 타당하지 않으면, 공사현장의 안전성을 정확히 판단할 수 없으므로 관리기준치의 선정은 매우 중요하다. 그러나 실질적으로 현장 기술자 등은 기존 절대치관리기준치에 대한 신뢰성이 매우 낮으며 관리기준치의 수정보완의 필요성을 인식하고 있다. 이에 본 연구에서는 계측관리기준치의 수정보완 필요성에 대한 조사결과를 바탕으로 하여 흙막이 구조물의 거동 중 가장 기본이 되며 중요한 흙막이 벽체의 변위에 대한 계측결과를 토대로 확률론적 이론에 의해 관리기준치를 보다 적절하게 설정하여 적용하는 연구를 수행하였다. 본 연구에서는 계측관리기법 중 시공 전에 설정된 관리기준치와 시공 시 측정된 설측치를 비교 검토하여 그 시점에서 공사의 안전성을 확인하는 방법인 절대치관리방법을 적용하여 관리기준치를 수정하였다.

• Journal of Microbiology and Biotechnology
• /
• 제28권9호
• /
• pp.1482-1492
• /
• 2018

Fusarium oxysporum trypsin (FOT) is a fungal serine protease similar to mammal trypsin. The FOT could be successfully expressed in Pichiapastoris by engineering the natural propeptide APQEIPN. In this study, we constructed two recombinant enzymes with engineered amino acid sequences added to the N-terminus of FOT and expressed in P. pastoris. The N-terminal residues had various effects on the structural and functional properties of trypsin. The FOT, and the recombinants TE (with peptide YVEF) and TS (with peptide YV) displayed the same optimum temperature ($40^{\circ}C$) and pH (8.0). However, the combinants TE and TS showed significantly increased thermal stability at $40^{\circ}C$ and $50^{\circ}C$. Moreover, the combinants TE and TS also showed enhanced tolerance of alkaline pH conditions. Compared with those of wild-type FOT, the intramolecular hydrogen bonds and the cation ${\pi}$-interactions of the recombinants TE and TS were significantly increased. The recombinants TE and TS also had significantly increased catalytic efficiencies (referring to the specificity constant, $k_{cat}/K_m$), 1.75-fold and 1.23-fold than wild-type FOT. In silico modeling analysis uncovered that the introduction of the peptides YVEF and YV resulted in shorter distances between the substrate binding pocket (D174, G198, and G208) and catalytic triad (His42, Asp102, and Ser180), which would improve the electron transfer rate and catalytic efficiency. In addition, N-terminal residues modification described here may be a useful approach for improving the catalytic efficiencies and characteristics of other target enzymes.

• Journal of Microbiology and Biotechnology
• /
• 제26권7호
• /
• pp.1163-1172
• /
• 2016

The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T_m values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

• 한국강구조학회 논문집
• /
• 제10권1호통권34호
• /
• pp.125-135
• /
• 1998

본 연구에서는 내측지점의 상승 하강을 이용하여 부모멘트구간에 프리스트레스를 도입하는 3경간 연속 프리플렉스 합성형교의 설계방법을 제시하고 이를 전산 프로그램화하여 최적설계를 수행하였다. 최적설계를 위한 목적함수로는 3경간 연속 프리플렉스 합성형교의 재료비용으로 결정하고, 제약방정식은 설계과정 및 시방서에 명시된 최소 설계단면제원과 부재별 허용응력에 근거하여 구성하였다. 최적설계기법으로는 기존의 2경간 최적화 연구에서 제시된 수치적 최적화기법의 하나인 순차 비제약 최소화기법을 재검토 및 수정하여 설계를 수행하였다. 민감도분석 결과에 근거하여 지간길이에 따른 최적설계단면을 결정하고, 여기서 정해진 최종 설계단면을 단순보형 프리플렉스 합성형교의 설계단면과 비교하였다. 그리고 각 지간별 합성보의 형고를 변화시키면서 최적화를 수행하여 형고와 재료비용과의 관계도를 제시하고 최적형고를 결정하였다.