• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.861-868
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• 2020

The use of polyphenol extracts from radiata pine (Pinus radiata) bark as raw materials for bio-foams was investigated along with the optimal NaOH extraction conditions. The targeted yield of alkaline extracts was 60%, and the targeted pH was 11 to 12. The radiata pine bark was composed of 70% of a 1% NaOH extract, which contained mainly polyphenols, such as proanthocyanidin (PA). As the particle size of the bark decreased, the yield of the 1% NaOH extracts increased from 57 to 87%. A range of NaOH concentrations, liquor ratios, and extraction times were explored to establish an economic polyphenol extraction method. More than 60% of the alkaline extract was extracted, and the pH of the extract was approximately 12 when the optimum extraction conditions were employed, i.e., a liquor to bark ratio of 5:1, a NaOH dosage of 17 to 18% based on the bark weight, and a 1 h extraction time. Following neutralization of the alkaline extract, structural analysis indicated severe structural changes in the PA during the alkaline extraction. Because the alkaline extract was barely soluble in the solvent used for the structural analyses, it is assumed that chemical modification is required to increase the solubility of the alkaline extract for the production of bio-foams.

• Journal of Korean Society of Steel Construction
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• v.12 no.6
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• pp.629-638
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• 2000

With the positive features of simulated annealing algorithms such as simplicity of the algorithm and the possibility of finding global optimum solution, SA algorithm has been widely applied to structural optimization problems. However, the algorithms are far from practical applications in structural design or optimization of building structures due to requirement of a large number of iterations and dependency on cooling schedule and stopping criteria. In this paper, with the modification of annealing process and stopping criteria, a MSA algorithm is presented in the form of two phase annealing process for optimal seismic design of braced structures. The performance of the proposed algorithm has been illustrated in detail.

• Transactions of the Korean Society of Automotive Engineers
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• v.5 no.3
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• pp.182-190
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• 1997

Recently to develope an automobile with better properties, much researches and investments are executed in many countries. In this paper, it is intended to minimize the weight of an engine block without changing the dynamic characteristics. The weight minimization is executed by the sensitivity of the natural frequency of the engine block. To decrease the engine weight, much less thickness than the original thickness of the engine is selected to initial value and the structure modification is performed to recover the dynamic characteristics of the engine. Here, the original thickness of the engine is 8mm and the initial thickness is selected to 5mm, 6mm and the number if the natural frequencies fitted are 2, 6, 7, respectively. As the results, it is found that; (1) the weight of each case could be reduced without changing the objective natural frequencies. Specially, in the case of fitting 2 natural frequencies with 5mm initial thickness the weight could be reduced to 4.21kg(23.3% for engine weight). (2) according to the driving frequency range of engine, the weight minimization could be performed choicely, (3) improving a vibration characteristics of a orignal structure, the weight minimization could be performed.

• Structural Engineering and Mechanics
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• v.52 no.1
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• pp.205-220
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• 2014

This paper is concerned with the modification of multidisciplinary feasible formulation for MDO problems using the integrated coupled approximate models. A drawback of conventional MDFs is the numerical difficulty in decomposing the design variables and deriving the coupled equations of state. To overcome such a drawback of conventional methods, the coupling in analysis and design is resolved by approximating the state variables in each discipline by the response surface method and by modifying the optimization formulation using the corresponding integrated coupled approximate models. The validity, reliability and effectiveness of the proposed method are illustrated and verified through two optimization problems, a mathematical MDF problem and the multidisciplinary optimum design of suspension unit of wheeled armored vehicle.

• Clean Technology
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• v.25 no.1
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• pp.33-39
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• 2019

A.C (activated carbon) is mainly used to remove VOCs (volatile organic compounds), however, it has many problems such as fire risk due to increasing of adsorbent surface temperature during VOCs ad/desorption, increased cost by frequent replacement cycles requirement and performance degradation when containing moisture. In order to solve these problems, many researches, hydrophobic zeolite adsorbents, have been reported. In this study, $NH_4{^+}Y$-zeolite was synthesized with Y-zeolite through steam treatment and acid treatment, which is one of the hydrophobic modification methods, to secure high surface area, thermal stability and humidity resistance. The Y, Y-550-HN, Y-600-HN and Y-650-HN had adsorption capacities of $23mg\;g^{-1}$, $38mg\;g^{-1}$, $77mg\;g^{-1}$, $61mg\;g^{-1}$. The change of Si/Al ratio, which is an index to confirm the degree of modification, was confirmed by XRF (X-ray fluorescence spectrometer) analysis. As a result, the adsorbtion performance was improved when Y-zeolite modified, and the Si/Al ratio of Y, Y-550-HN, Y-600-HN, Y-650-HN were increased to 3.1765, 6.6706, 7.3079, and 7.4635, respectively. Whereas it was confirmed that structural crystallization due to high heat treatment temperature affected performance degradation. Therefore, there is an optimal heat treatment temperature of Y-zeolite, optimum modification condition study could be a substitute for activated carbon as a condition for producing an adsorbent having high durability and stability.

• Earthquakes and Structures
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• v.19 no.3
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• pp.197-214
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• 2020

Diagrid structures have been introduced as a fairly modern lateral load-resisting system in the design of high-rise buildings. In this paper, a novel diagrid system called tube-in-tube diagrid building is introduced and assessed through pushover and incremental dynamic analyses. The main objectives of this paper are to find the optimum angle of interior and exterior diagrid tube and evaluate the efficiency of diagrid core on the probability of collapse comparing to the conventional diagrid system. Finally, the seismic performance factors of the proposed system are validated according to the FEMA P695 methodology. To achieve these, 36-story diagrid buildings with various external and internal diagonal angles are designed and then 3-D nonlinear models of these structures developed in PERFORM-3D. The results show that weight of steel material highly depends on diagonal angle of exterior tube. Adding diagrid core generally increases the over-strength factor and collapse margin ratio of tall diagrid buildings confirming high seismic safety margin for tube-in-tube diagrid buildings under severe excitations. Collapse probabilities of both structural systems under MCE records are less than 10%. Finally, response modification factor of 3.0 and over-strength factor of 2.0 and 2.5 are proposed for design of typical diagrid and tube-in-tube diagrid buildings, respectively.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.1
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• pp.95-103
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• 2016

The absolute value management method is widely used in the most of the earth retaining construction, which evaluates the safety by comparing measurement result and management criteria. Therefore, the management criteria is the standard to evaluate the safety of the site, and in other words, the criteria is a direct factor of the evaluation. That means that the safety of the site can not be acquired if the management criteria is not proper, even though the measurement system is perfectly set. However, many of field technicians do not have rely on the current management criteria, and they even recognize the necessity of the revision. Therefore, in this study, the necessity of the revision was studied. Also, the optimum criteria selection and the application were performed based on the test results of earth retaining deflection and probabilistic theory. The absolute value management method was used for this study. The details are tabulated.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1482-1492
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• 2018

Fusarium oxysporum trypsin (FOT) is a fungal serine protease similar to mammal trypsin. The FOT could be successfully expressed in Pichiapastoris by engineering the natural propeptide APQEIPN. In this study, we constructed two recombinant enzymes with engineered amino acid sequences added to the N-terminus of FOT and expressed in P. pastoris. The N-terminal residues had various effects on the structural and functional properties of trypsin. The FOT, and the recombinants TE (with peptide YVEF) and TS (with peptide YV) displayed the same optimum temperature ($40^{\circ}C$) and pH (8.0). However, the combinants TE and TS showed significantly increased thermal stability at $40^{\circ}C$ and $50^{\circ}C$. Moreover, the combinants TE and TS also showed enhanced tolerance of alkaline pH conditions. Compared with those of wild-type FOT, the intramolecular hydrogen bonds and the cation ${\pi}$-interactions of the recombinants TE and TS were significantly increased. The recombinants TE and TS also had significantly increased catalytic efficiencies (referring to the specificity constant, $k_{cat}/K_m$), 1.75-fold and 1.23-fold than wild-type FOT. In silico modeling analysis uncovered that the introduction of the peptides YVEF and YV resulted in shorter distances between the substrate binding pocket (D174, G198, and G208) and catalytic triad (His42, Asp102, and Ser180), which would improve the electron transfer rate and catalytic efficiency. In addition, N-terminal residues modification described here may be a useful approach for improving the catalytic efficiencies and characteristics of other target enzymes.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1163-1172
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• 2016

The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T_m values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

• Journal of Korean Society of Steel Construction
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• v.10 no.1 s.34
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• pp.125-135
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• 1998

This study presents a design method for three-span continuous preflex composite girder bridges (3S-PCB) which imposes prestresses in the negative moment region by lifting or lowering interior supports and the design method is automated by a computer program which incorporates optimal design procedure. The objective function for the design of 3S-PCB minimizes the cost of construction materials and the constraint functions represent the limited dimensions of the design section and the allowable stress for each structural member as given in the specifications. Optimal design procedure used in this study is a modification of existing sequential unconstrained minimization technique (SUMT), a numerical analyses procedure for two-span continuous preflex composite bridges. The optimized design sections determined for each span length are compared with those of simple preflex composite beams (SPCB) and the optimal girder depth is determined by defining the relationship between girder depth and construction material costs.