• Archives of Pharmacal Research
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• 제20권1호
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• pp.46-52
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• 1997

To obtain more sensitive immunoassay for methamphetamine (MA) determination, the optimum condition of enzyme-linked immunosorbent assay (ELISA) was investigated in regard to immunogens, antibody purification methods and coating tracers. Activated MA, N-(4-aminobutyl)methamphetamine (4-ABMA), was conjugated with bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH) and used as immunogen. The antibodies were purified by protein G chromatography or various immunoaffinity chromatography-linked MA-protein ligands, such as MA-BSA, MA-KLH or MA-ovalbumin (OVA). Each purified antibody was characterized by means of sensitivity and cross-reactivity using the three MA-protein coating tracers, MA-BSA, MA-KLH and MA-OVA. The best sensitivity of each antibody was acquired with the MA-OVA tracer although the tracer concentration and the antibody titer level at optimum condition were varied. The antibody with high titer level did not always yield good sensitivity. At optimum condition, immunoaffinity chromatography-purified antibodies were better for sensitivity and for specificity than protein G-purified antibodies. The cross-reactivity of the purified antibodies seemed to be affected by immunogen structure and showed somewhat different patterns according to the immunoaffinity ligand utilized. These data show that the antibody purification method as well as choice of coating tracer and immunogen is essential for the sensitivity and specificity of EIA; the optimum condition for assay should be discovered using various methods and combinations.

• Journal of Advanced Marine Engineering and Technology
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• 제26권1호
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• pp.48-58
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• 2002

There is a purpose of this study for the proposal of the optimum technique utilized for the vibration design initial step. The stiffened plate structure for the ship hull is made for analysis model. To begin with, dynamic characteristics of stiffened plate structure is analysed using FEM. Main vibrational mode of the structure is decided in the analytical result of FEM. The simplified equation on the natural frequency of the main vibrational mode is induced. Next, sensitivity analysis is carried out using the simplified equation, and rate of change of dynamic characteristics is calculated. Then, amount of design variable is calculated using this sensitivity value and optimum structural modification method. The change of natural frequency is made to be an objective function. Thickness of panel, cross section moment of stiffener and girder become a design variable. The validity of the optimization method using simplified equation is examined. It is shown that the result effective in the optimum modification for natural frequency of the stiffened plate structure.

• 한국항해학회지
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• 제25권1호
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• pp.45-52
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• 2001

본 논문은 선체구조에 많이 이용되고 있는 보강판 구조물의 동적 특성을 최적 변경하는데 그 목적이 있다. 유한요소법(FEM), 동적 감도해석법, 최적구조 변경법을 이용하여 보강판의 동적 특성을 최적화한다. 먼저, FEM을 이용하여 보강판 구조물의 동적 특성을 해석한다. 다음으로 설계변수의 변화에 따른 동적 특성의 변화율을 동적 감도해석법으로 해석한다. 감도해석법으로 구한 감도값과 최적구조 변경법을 이용하여 설계변수들의 변경 량을 계산한다. 보강판 구조물의 고유진동수의 변경을 목적함수로 하고, 보강판의 두께와 보강재의 단면2차 모우멘트를 설계 변수로 한다. 본 논문에서 이용한 최적구조 변경법이 보강판 구조물의 동특성을 최적화하는데 유용함을 보여준다.

• 한국생산제조학회지
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• 제18권4호
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• pp.355-361
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• 2009

In this paper, an analysis technique is presented for performing the effective design of bus structure. Sensitivity analysis is carried out for the natural frequency of component structures consisting of bus B.I.W. Local vibration modes of substructure, which large affect on the global vibration mode of the bus B.I.W., are obtained through the sensitivity analysis technique using the mathematical chain rule. And also the design variables, which are determined from the sensitivity analysis, are redesigned through optimum design process. The proposed analysis technique shows that the bus structure can be effectively designed considering the vibration characteristics.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.354-357
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• 2004

In this paper, a sensitivity analysis technique is presented for performing effective structural optimization of bus system. Design sensitivities are analyzed on natural frequency of bus substructures using super-element. Vibration modes of substructure, which large affect on the global vibration mode of bus B.I.W., are found through the sensitivity analysis using the chain rule. And design variables, which are determined from the sensitivity analysis, are changed through optimum design.

• 대한조선학회논문집
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• 제51권1호
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• pp.78-87
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• 2014

Recently the offshore wind turbine development is requested to be installed off south-west coast and Jeju island in Korea. Reliable and robust support structures are required to meet the demand on the offshore wind turbine in harsh and rapidly varying environmental conditions. Monopile is the most preferred substructure in shallow water with long term experiences from the offshore gas and oil industries. This paper presents an optimum design of a monopile connection with grouted transition piece (TP) for the reliable and cost-effective design purposes. First, design loads are simulated for a 5 MW offshore wind turbine in site conditions off the southwest coast of Korea. Second, sensitivity analysis is performed to investigate the design sensitivity of geometry and material parameters of monopile connection based on the ultimate and fatigue capacities according to DNV standards. Next, optimization is conducted to minimize the total mass and resulted in 30% weight reduction and the optimum geometry and material properties of the monopile substructure of the fixed offshore wind turbine.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 1998년도 춘계학술발표회 논문집
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• pp.120-127
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• 1998

It is very difficult to execute the vibration analysis of a huge strucutre, which takes up much time and expense. In this paper we intend to make the equivalent system of a local vibration system of a huge structure with a view to improving the dynamic characteristics and reducing time and expense. First of all, upper deck structure model is maded. And we perform the vibration analysis by the Substructure Synthesis Method and execute the exciting test for the upper deck structure model, and observe the coincidences of two results to confirm the reliability of the analyzing tools used. To make the equivalent system, we give boundary condition to sub-structure that want to be modified and execute the Sensitivity Analysis Method and the Optimum Structural Modification Method. And we execute the structural modification of the equivalent system.. The following can be found from this study. 1. The analytical results are generally coincident with each other. 2. The equivalent system of the superstructure model can be easily obtained using the sensitivity analysis metho and the optimum structural modification method. 3. The structural modification using the equivalent system can be obtained good results above 90% of object value.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 학술대회 논문집 정보 및 제어부문
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• pp.167-169
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• 2005

This paper proposes an optimum tuning which improves the tuning effect of IMC-PID and guarantees the performance and robustness of controller system by considering gain margin, phase margin, sensitivity functions and integral square error(ISE) for IMC-PID controller.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2000년도 가을 학술발표회논문집(I)
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• pp.205-210
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• 2000

An optimum design algorithm of PSC box girder bridges using design sensitivity analysis is proposed in this paper. For the efficiency of the proposed algorithm, approximated reanalysis techniques using design sensitivity analysis are introduced. And also to save the numerical efforts, an efficient reanalysis technique through approximated structural responses is proposed. A design sensitivity analysis of structural response is executed by automatic differentiation(AD). The efficiency and robustness of the proposed algorithm, compared with conventional algorithm, is successfully demonstrated in the numerical example.

• 상하수도학회지
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• 제12권2호
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• pp.50-58
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• 1998

Genetic Algorithm (GA) consists of selection, reproduction, crossover and mutation processes and many parameters including population size, generation number, the probability of crossover (Pc) and the probability of mutation (Pm). Determining values of the parameters is found critical in the whole optimization process and a sensitivity analysis with them seems mandatory. This paper tries to demonstrate such importance of sensitivity analysis of GA using an example water supply tunnel network of the New York City. For optimization of the network with GA, Pc and Pm vary from 0.5 to 0.9 by an increment of 0.1 and from 0.01 to 0.05 by an increment of 0.01, respectively, while fixing both the population size and the generation number to 100. This sensitivity analysis results in an optimum design of 22.3879 million dollars at the values of 0.8 and 0.01 for Pc and Pm, respectively. In addition, the probability of recombination (Pr) is introduced to check its applicability in the GA optimization of water distribution network. When Pr is 0.05 with the same values of Pc and Pm as above, the optimum design costs 20.9077 million dollars. This is lower than the cost of 22.3879 million dollars for the case of not using Pr by 6.6%. These results indicate that conducting a sensitivity analysis with parameter values and using Pr are useful in the optimization of WDN.